PL2591102T3 - Procedura specyficznej izolacji całkowitej zawartości dna drobnoustrojów bakteryjnych i zestaw do tego celu - Google Patents

Procedura specyficznej izolacji całkowitej zawartości dna drobnoustrojów bakteryjnych i zestaw do tego celu

Info

Publication number
PL2591102T3
PL2591102T3 PL10735085T PL10735085T PL2591102T3 PL 2591102 T3 PL2591102 T3 PL 2591102T3 PL 10735085 T PL10735085 T PL 10735085T PL 10735085 T PL10735085 T PL 10735085T PL 2591102 T3 PL2591102 T3 PL 2591102T3
Authority
PL
Poland
Prior art keywords
kit
procedure
total dna
dna content
specific isolation
Prior art date
Application number
PL10735085T
Other languages
English (en)
Inventor
Gábor Kiss
János Kiss
Katalin Sztancsik
Georgina Bernáth
Original Assignee
Diagon Kft
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Diagon Kft filed Critical Diagon Kft
Publication of PL2591102T3 publication Critical patent/PL2591102T3/pl

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
PL10735085T 2010-07-07 2010-07-07 Procedura specyficznej izolacji całkowitej zawartości dna drobnoustrojów bakteryjnych i zestaw do tego celu PL2591102T3 (pl)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
PCT/HU2010/000077 WO2012004619A1 (en) 2010-07-07 2010-07-07 Procedure for the specific isolation of total dna content of bacterial germs and a kit for this purpose
EP10735085.2A EP2591102B1 (en) 2010-07-07 2010-07-07 Procedure for the specific isolation of total dna content of bacterial germs and a kit for this purpose

Publications (1)

Publication Number Publication Date
PL2591102T3 true PL2591102T3 (pl) 2015-11-30

Family

ID=42562687

Family Applications (1)

Application Number Title Priority Date Filing Date
PL10735085T PL2591102T3 (pl) 2010-07-07 2010-07-07 Procedura specyficznej izolacji całkowitej zawartości dna drobnoustrojów bakteryjnych i zestaw do tego celu

Country Status (8)

Country Link
US (2) US9024008B2 (pl)
EP (1) EP2591102B1 (pl)
CN (1) CN102906262A (pl)
BR (1) BR112012031267A2 (pl)
ES (1) ES2543367T3 (pl)
PL (1) PL2591102T3 (pl)
RU (1) RU2567809C2 (pl)
WO (1) WO2012004619A1 (pl)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9230185B1 (en) * 2012-03-30 2016-01-05 Pierce Biotechnology, Inc. Analysis of electrophoretic bands in a substrate
EP3235171B1 (en) * 2014-12-17 2020-10-14 Hewlett-Packard Development Company, L.P. Host a conference call
CN104673914A (zh) * 2015-02-13 2015-06-03 重庆京因生物科技有限责任公司 用于基因快速检测的细胞裂解液
CN105543399B (zh) * 2016-02-26 2019-07-16 中国科学院水生生物研究所 一种利用荧光检测微囊藻细胞dna损伤的方法
US11959125B2 (en) 2016-09-15 2024-04-16 Sun Genomics, Inc. Universal method for extracting nucleic acid molecules from a diverse population of one or more types of microbes in a sample
US10428370B2 (en) * 2016-09-15 2019-10-01 Sun Genomics, Inc. Universal method for extracting nucleic acid molecules from a diverse population of one or more types of microbes in a sample
US12072333B2 (en) 2016-11-02 2024-08-27 University Of Iowa Research Foundation Method and apparatus for selective removal of cells from a cell suspension by mechanical lysis
WO2019067388A1 (en) * 2017-09-30 2019-04-04 The Regents Of The University Of California EXTRACTION OF SHORT NUCLEIC ACIDS BY NANOFIBERS
CN109306370A (zh) * 2018-09-26 2019-02-05 浙江海洋大学 纳米材料胁迫下桡足类dna损伤的检测方法
CN109306350A (zh) * 2018-10-31 2019-02-05 宁波奇天基因科技有限公司 一种核酸释放剂以及核酸现场释放方法
CN113088454A (zh) * 2021-04-08 2021-07-09 武汉轻工大学 一种从被感染细胞中分离完整细菌的方法

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4900677A (en) 1986-09-26 1990-02-13 E. I. Du Pont De Nemours And Company Process for rapid isolation of high molecular weight DNA
US5234809A (en) 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
DE3935098C2 (de) 1989-10-21 1995-05-24 Macherey Nagel & Co Chem Chromatographisches Trägermaterial sowie seine Verwendung in einem Verfahren zur chromatographischen Trennung von Nukleinsäuren
IL110463A0 (en) 1993-08-13 1994-10-21 Du Pont In situ extraction of microbial DNA
JP3761573B2 (ja) 1994-06-14 2006-03-29 インヴィテーク ゲーエムベーハー 極度に少量でかつ非常に強く汚染された非常に種々の出発物質から核酸を単離および精製する一般的方法
DE4422044A1 (de) 1994-06-14 1995-12-21 Invitek Gmbh Verfahren zur Isolierung, Reinigung und ggf. Lagerung von Nukleinsäuren
US5576196A (en) * 1995-01-13 1996-11-19 Vical Incorporated Process for reducing RNA concentration in a mixture of biological material using diatomaceous earth
US5945515A (en) 1995-07-31 1999-08-31 Chomczynski; Piotr Product and process for isolating DNA, RNA and proteins
WO1998023630A1 (en) 1996-11-27 1998-06-04 Edge Biosystems, Inc. Matrix for rapid purification of nucleic acids
US7078224B1 (en) 1999-05-14 2006-07-18 Promega Corporation Cell concentration and lysate clearance using paramagnetic particles
DE19859703B4 (de) 1998-12-23 2009-10-29 Macherey, Nagel Gmbh & Co. Handelsgesellschaft Verfahren zur Aufreinigung von Nukleinsäuren sowie Anionenaustauscher zur Durchführung dieses Verfahrens
GB0015777D0 (en) 2000-06-28 2000-08-16 Tayside University Hospitals N DNA Extraction
WO2003046146A2 (en) 2001-11-28 2003-06-05 Applera Corporation Compositions and methods of selective nucleic acid isolation
EP1581646A4 (en) 2002-10-07 2006-07-05 Marligen Biosciences Inc EXTRACTION OF DNA FROM BIOLOGICAL SAMPLES
AU2002952858A0 (en) 2002-11-20 2002-12-05 Bio-Molecular Holdings Pty Limited Dna isolation material and method
DE602004030785D1 (de) * 2003-05-19 2011-02-10 Univ Brandeis Verfahren, kits und vorrichtungen zur prozessierung von nukleinsäuren
CA2629589C (en) 2007-04-20 2016-03-29 F.Hoffmann-La Roche Ag Isolation and purification of nucleic acid molecules with a solid phase

Also Published As

Publication number Publication date
ES2543367T3 (es) 2015-08-18
WO2012004619A1 (en) 2012-01-12
US20150376601A1 (en) 2015-12-31
EP2591102B1 (en) 2015-05-13
RU2013104648A (ru) 2014-08-20
US20130109027A1 (en) 2013-05-02
EP2591102A1 (en) 2013-05-15
US9024008B2 (en) 2015-05-05
US9605256B2 (en) 2017-03-28
RU2567809C2 (ru) 2015-11-10
CN102906262A (zh) 2013-01-30
BR112012031267A2 (pt) 2015-10-13

Similar Documents

Publication Publication Date Title
PL2591102T3 (pl) Procedura specyficznej izolacji całkowitej zawartości dna drobnoustrojów bakteryjnych i zestaw do tego celu
SG10201503501TA (en) Microorganisms and methods for the biosynthesis of butadiene
ZA201300612B (en) Novel bacteria and methods of use thereof
ZA201303499B (en) Specific diarylhydantoin and diarylthiohydantion compounds
GB0910144D0 (en) Pipeline isolation
ZA201105741B (en) Methods for isolating bacteria
HU0900819D0 (en) Lung tissue culture
GB2495679B (en) Pipeline isolation
IL214979A0 (en) Compositions and methods for elimination of gram-negative bacteria
GB201101671D0 (en) Virtual concentric manual torque wrench with off-axis compensation
GB0922097D0 (en) Microbial assay
GB2477597B (en) Disinfectant materials and methods
PL2276498T3 (pl) Zastosowanie bakterii probiotycznych do leczenia hiperhomocysteinemii
GB201019086D0 (en) Bacterial methods
EP2472534A4 (en) Transformer
GB0902173D0 (en) Compounds and their use
ZA201108490B (en) Fermentation
GB0817240D0 (en) Modified bacterial cell
GB201007379D0 (en) Bacterial secretion
ZA201001225B (en) Methods of culturing bacterial species of the anaplasmataceae family
AU2009900064A0 (en) Experimental equipment for living organisms
HU0900278D0 (en) Lung tissue culture
HK1184329A (en) Oligo-benzamide compounds and their use
HK1172319A (en) Therapeutic aryl-am i do-aryl compounds and their use
PL387418A1 (pl) Preparat mineralno-bakteryjny i sposób jego otrzymywania