PL317761A1 - In yeast exprimed proteins elongated at the end n - Google Patents

In yeast exprimed proteins elongated at the end n

Info

Publication number
PL317761A1
PL317761A1 PL95317761A PL31776195A PL317761A1 PL 317761 A1 PL317761 A1 PL 317761A1 PL 95317761 A PL95317761 A PL 95317761A PL 31776195 A PL31776195 A PL 31776195A PL 317761 A1 PL317761 A1 PL 317761A1
Authority
PL
Poland
Prior art keywords
exprimed
yeast
proteins
elongated
yeast exprimed
Prior art date
Application number
PL95317761A
Inventor
Knud Vad
Jakob Brandt
Thomas Borglum Kjeldsen
Original Assignee
Novo Nordisk As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk As filed Critical Novo Nordisk As
Publication of PL317761A1 publication Critical patent/PL317761A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Diabetes (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
PL95317761A 1994-06-17 1995-06-16 In yeast exprimed proteins elongated at the end n PL317761A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK71294 1994-06-17
PCT/DK1995/000250 WO1995035384A1 (en) 1994-06-17 1995-06-16 N-terminally extended proteins expressed in yeast

Publications (1)

Publication Number Publication Date
PL317761A1 true PL317761A1 (en) 1997-04-28

Family

ID=8096676

Family Applications (1)

Application Number Title Priority Date Filing Date
PL95317761A PL317761A1 (en) 1994-06-17 1995-06-16 In yeast exprimed proteins elongated at the end n

Country Status (16)

Country Link
EP (1) EP0765395A1 (en)
JP (1) JP3730255B2 (en)
CN (1) CN1131312C (en)
AU (1) AU2733595A (en)
BR (1) BR9508053A (en)
CA (1) CA2192943A1 (en)
CZ (1) CZ363796A3 (en)
FI (1) FI965030A7 (en)
HU (1) HUT75840A (en)
IL (1) IL114160A (en)
MX (1) MX9606536A (en)
NO (1) NO965411L (en)
PL (1) PL317761A1 (en)
SG (1) SG47883A1 (en)
WO (1) WO1995035384A1 (en)
ZA (1) ZA954983B (en)

Families Citing this family (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6500645B1 (en) 1994-06-17 2002-12-31 Novo Nordisk A/S N-terminally extended proteins expressed in yeast
ZA9610456B (en) * 1995-12-20 1997-06-20 Novo Nordisk As N-terminally extended proteins expressed in yeast
DE69725881T2 (en) * 1996-12-13 2004-09-09 Chiron Corp. (N.D.Ges.D. Staates Delaware), Emeryville METHOD FOR EXPRESSING HETEROLOGICAL PROTEINS IN YEAST
EP0946735B1 (en) * 1996-12-20 2006-02-08 Novo Nordisk A/S N-terminally extended proteins expressed in yeast
JP2003525570A (en) * 1998-01-23 2003-09-02 ノボ ノルディスク アクティーゼルスカブ Method for producing desired polypeptide in yeast
EP1246933B1 (en) 1999-12-29 2010-07-21 Novo Nordisk A/S Method for making insulin precursors and insulin precursor analogues having improved fermentation yield in yeast
AU2002355160A1 (en) * 2001-07-24 2003-02-17 Novo Nordisk A/S Method for making acylated polypeptides
US7595172B2 (en) 2001-07-24 2009-09-29 Novo Nordisk A/S Method for making acylated polypeptides
US20030082671A1 (en) 2001-07-24 2003-05-01 Thomas Hoeg-Jensen Method for making acylated polypeptides
WO2004085472A1 (en) * 2003-03-27 2004-10-07 Novo Nordisk A/S Method for making human insulin precursors and human insulin
CN1902226A (en) 2003-12-03 2007-01-24 诺和诺德公司 Single-chain insulin
CN102660614A (en) 2005-08-16 2012-09-12 诺沃-诺迪斯克有限公司 Method for making mature insulin polypeptides
ES2542146T3 (en) * 2006-07-31 2015-07-31 Novo Nordisk A/S PEGylated extended insulin.
DK2074141T3 (en) 2006-09-22 2016-11-28 Novo Nordisk As The protease resistant insulin analogues.
EP2069502B1 (en) 2006-09-27 2014-02-26 Novo Nordisk A/S Method for making maturated insulin polypeptides
EP2084179B1 (en) 2006-11-22 2013-05-22 Novo Nordisk A/S Method for making activated carboxypeptidases
WO2009010428A1 (en) 2007-07-16 2009-01-22 Novo Nordisk A/S Protease stabilized, pegylated insulin analogues
JP5710253B2 (en) 2007-08-13 2015-04-30 ノボ・ノルデイスク・エー／エス Fast-acting insulin analogue
EP2178910B1 (en) 2007-08-15 2014-10-08 Novo Nordisk A/S Insulins with an acyl moiety comprising repeating units of alkylene glycol containing amino acids
KR20100053561A (en) 2007-08-15 2010-05-20 노보 노르디스크 에이/에스 Insulin analogues with an acyl and alkylene glycol moiety
CN102037008B (en) 2008-03-18 2016-08-31 诺沃-诺迪斯克有限公司 Protease-stabilized, acylated insulin analogs
ES2563553T3 (en) 2008-04-01 2016-03-15 Novo Nordisk A/S Insulin-albumin conjugates
AU2010264636B2 (en) 2009-06-26 2013-06-27 Novo Nordisk A/S Preparation comprising insulin, nicotinamide and an amino acid
US8815541B2 (en) 2009-11-25 2014-08-26 Novo Nordisk A/S Method for making polypeptides
CN102933599A (en) 2010-06-23 2013-02-13 诺沃—诺迪斯克有限公司 Human insulin containing additional disulfide bonds
RU2598273C2 (en) 2010-06-23 2016-09-20 Ново Нордиск А/С Insulin derivatives containing additional disulfide bonds
EP2585484A1 (en) 2010-06-23 2013-05-01 Novo Nordisk A/S Insulin analogues containing additional disulfide bonds
JP2013545782A (en) 2010-12-14 2013-12-26 ノヴォ ノルディスク アー／エス Fast-acting insulin combined with long-acting insulin
MX2013006174A (en) 2010-12-14 2013-07-15 Novo Nordisk As Preparation comprising insulin, nicotinamide and an amino acid.
CN103596584B (en) 2011-06-15 2016-12-14 诺沃—诺迪斯克有限公司 Polysubstituted insulin
WO2013186138A1 (en) 2012-06-14 2013-12-19 Novo Nordisk A/S Preparation comprising insulin, nicotinamide and arginine
US9707276B2 (en) 2012-12-03 2017-07-18 Merck Sharp & Dohme Corp. O-glycosylated carboxy terminal portion (CTP) peptide-based insulin and insulin analogues
JP2016521701A (en) 2013-06-07 2016-07-25 ノヴォ ノルディスク アー／エス Methods for making mature insulin polypeptides
US20160376330A1 (en) 2014-02-28 2016-12-29 Novo Nordisk A/S Mating factor alpha pro-peptide variants
JP6983075B2 (en) 2015-06-02 2021-12-17 ノヴォ ノルディスク アー／エス Insulin with polar recombination extension
JP2018531899A (en) 2015-08-25 2018-11-01 ノヴォ ノルディスク アー／エス Novel insulin derivatives and their medical use
EP3341401A1 (en) 2015-08-25 2018-07-04 Novo Nordisk A/S Novel insulin derivatives and the medical uses hereof
MX2018002223A (en) 2015-08-25 2018-03-23 Novo Nordisk As Novel insulin derivatives and the medical uses hereof.
CN109414471A (en) * 2016-05-24 2019-03-01 诺和诺德股份有限公司 MIC-1 compound and application thereof
CN111032685A (en) 2017-08-17 2020-04-17 诺沃挪第克公司 Novel acylated insulin analogues and uses thereof
CN112105635A (en) * 2018-06-18 2020-12-18 联合化学实验室有限公司 Leader sequences for higher expression of recombinant proteins
CN110305223B (en) * 2019-06-26 2022-05-13 重庆派金生物科技有限公司 Method for preparing target polypeptide by recombinant tandem fusion protein
EP4480492A3 (en) 2019-12-11 2025-03-19 Novo Nordisk A/S Novel insulin analogues and uses thereof
WO2023144240A1 (en) 2022-01-26 2023-08-03 Novo Nordisk Research Centre Oxford Limited Glucose sensitive insulin derivatives and uses thereof

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2593518B1 (en) * 1985-05-02 1989-09-08 Transgene Sa VECTORS FOR THE EXPRESSION AND SECRETION OF HIRUDIN BY TRANSFORMED YEASTS
US5010182A (en) * 1987-07-28 1991-04-23 Chiron Corporation DNA constructs containing a Kluyveromyces alpha factor leader sequence for directing secretion of heterologous polypeptides
CA1340772C (en) * 1987-12-30 1999-09-28 Patricia Tekamp-Olson Expression and secretion of heterologous protiens in yeast employing truncated alpha-factor leader sequences
DK105489D0 (en) * 1989-03-03 1989-03-03 Novo Nordisk As POLYPEPTIDE
DK300090D0 (en) * 1990-12-19 1990-12-19 Novo Nordisk As PROCEDURE FOR PREPARING LEADER SEQUENCES
WO1992013951A1 (en) * 1991-02-04 1992-08-20 The Salk Institute Biotechnology/Industrial Associates, Inc. Production of human serum albumin in methylotrophic yeast cells

Also Published As

Publication number Publication date
HUT75840A (en) 1997-05-28
MX9606536A (en) 1997-03-29
NO965411D0 (en) 1996-12-16
CN1131312C (en) 2003-12-17
JP3730255B2 (en) 2005-12-21
CN1152942A (en) 1997-06-25
FI965030L (en) 1997-02-14
CZ363796A3 (en) 1997-05-14
NO965411L (en) 1997-02-14
EP0765395A1 (en) 1997-04-02
CA2192943A1 (en) 1995-12-28
FI965030A0 (en) 1996-12-16
IL114160A0 (en) 1995-10-31
FI965030A7 (en) 1997-02-14
HU9603476D0 (en) 1997-02-28
IL114160A (en) 2006-12-31
AU2733595A (en) 1996-01-15
BR9508053A (en) 1997-08-12
SG47883A1 (en) 1998-04-17
JPH10501695A (en) 1998-02-17
WO1995035384A1 (en) 1995-12-28
ZA954983B (en) 1996-02-14

Similar Documents

Publication Publication Date Title
PL317761A1 (en) In yeast exprimed proteins elongated at the end n
TW282480B (en)
BR9508234A (en)
GB2286821B (en) Improvements in counterbalancing on collapsible flatracks
GB9405629D0 (en) Recombinant DNA for use in yeast
IL115317A0 (en) Proteins
GB9423372D0 (en) Proteins and their uses
DE69519669D1 (en) Cyclodepsipeptide
GB9413316D0 (en) Novel proteins
EP0729975A4 (en) Ecdn protein and dna coding for the same
GB9425624D0 (en) Improvements in two stroke engines
GB9402906D0 (en) Improvements in swatches
EP0762914A4 (en) Improvements in racquets
GB9403188D0 (en) Improvements in canopies
AR255595A1 (en)
GB9411089D0 (en) Proteins
GB9424025D0 (en) Proteins
ZA957852B (en) Proteins
HU9400235V0 (en) Drink-paccked in boxes-pouring
GB9405657D0 (en) Improvements in screwdrivers
GB9411007D0 (en) Improvements in flylines
GB9420989D0 (en) Protein
AUPM730694A0 (en) Improvements in engines
AUPM967394A0 (en) Improvements in engines
GB9608728D0 (en) The all in one