PL321499A1 - Production of anzymatically active recombinative carboxypeptidase b - Google Patents

Production of anzymatically active recombinative carboxypeptidase b

Info

Publication number
PL321499A1
PL321499A1 PL96321499A PL32149996A PL321499A1 PL 321499 A1 PL321499 A1 PL 321499A1 PL 96321499 A PL96321499 A PL 96321499A PL 32149996 A PL32149996 A PL 32149996A PL 321499 A1 PL321499 A1 PL 321499A1
Authority
PL
Poland
Prior art keywords
procpb
enzymatically active
anzymatically
recombinative
carboxypeptidase
Prior art date
Application number
PL96321499A
Other versions
PL183228B1 (en
Inventor
Jacob Hartman
Netta Fulga
Simona Mendelovitch
Marian Gorecki
Original Assignee
Bio Technology General Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bio Technology General Corp filed Critical Bio Technology General Corp
Publication of PL321499A1 publication Critical patent/PL321499A1/en
Publication of PL183228B1 publication Critical patent/PL183228B1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Toxicology (AREA)
  • Diabetes (AREA)
  • Endocrinology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

The subject invention provides a method of producing enzymatically active CPB which comprises treating a recombinant cell containing DNA encoding ProCPB, so that the DNA directs expression of the ProCPB, recovering from the cell the ProCPB so expressed, treating the recovered ProCPB under conditions permitting folding of the ProCPB, subjecting the folded ProCPB to enzymatic cleavage to produce enzymatically active CPB and purifying the enzymatically active CPB.
PL96321499A 1995-01-25 1996-01-25 Production of anzymatically active recombinative carboxypeptidase b PL183228B1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US37823395A 1995-01-25 1995-01-25
PCT/US1996/000995 WO1996023064A1 (en) 1995-01-25 1996-01-25 Production of enzymatically active recombinant carboxypeptidase b

Publications (2)

Publication Number Publication Date
PL321499A1 true PL321499A1 (en) 1997-12-08
PL183228B1 PL183228B1 (en) 2002-06-28

Family

ID=23492292

Family Applications (1)

Application Number Title Priority Date Filing Date
PL96321499A PL183228B1 (en) 1995-01-25 1996-01-25 Production of anzymatically active recombinative carboxypeptidase b

Country Status (21)

Country Link
US (1) US5948668A (en)
EP (1) EP0871718B1 (en)
JP (1) JP4250716B2 (en)
KR (1) KR100566136B1 (en)
CN (1) CN1144874C (en)
AT (1) ATE358717T1 (en)
AU (1) AU698889B2 (en)
BR (1) BR9606795A (en)
CA (1) CA2210242C (en)
CZ (1) CZ292541B6 (en)
DE (1) DE69637011T2 (en)
DK (1) DK0871718T3 (en)
ES (1) ES2284167T3 (en)
HU (1) HU225673B1 (en)
IL (1) IL116696A (en)
MX (1) MX9705279A (en)
NZ (1) NZ302874A (en)
PL (1) PL183228B1 (en)
PT (1) PT871718E (en)
WO (1) WO1996023064A1 (en)
ZA (1) ZA96515B (en)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1193278A (en) 1995-08-16 1998-09-16 曾尼卡有限公司 Chemical compounds
ES2245114T3 (en) 1998-08-06 2005-12-16 Mountain View Pharmaceuticals, Inc. CONJUGATES OF PEG-OXIDASA DE URATO AND ITS USE.
US20040117863A1 (en) * 1998-09-18 2004-06-17 Edge Michael D. Transgenically produced fusion proteins
DE19915938A1 (en) * 1999-04-09 2000-10-19 Aventis Pharma Gmbh Production of pancreatic procarboxypeptidase B, isoforms and muteins thereof and their use
EP1220864A4 (en) * 1999-09-17 2003-05-21 Genzyme Transgenics Corp TRANSGENIC PRODUCED FUSION PROTEINS
EP1632575B1 (en) * 1999-09-29 2009-01-28 Lexicon Pharmaceuticals, Inc. Human carboxypeptidases and polynucleotides encoding the same
EP1220932B1 (en) 1999-09-29 2005-11-09 Lexicon Genetics Incorporated Human carboxypeptidases and polynucleotides encoding the same
WO2001051624A2 (en) * 2000-01-12 2001-07-19 Eli Lilly And Company Carboxypeptidase b free of animal products and contaminating enyzme activity
EP1538203B1 (en) * 2003-12-05 2010-01-20 Roche Diagnostics GmbH Recombinantly expressed carboxypeptidase B and purification thereof
ATE455849T1 (en) * 2003-12-05 2010-02-15 Hoffmann La Roche RECOMBINANT CARBOXYPEPTIDASE B AND ITS PURIFICATION
ES2369781T3 (en) * 2004-09-27 2011-12-05 Sanofi-Aventis Deutschland Gmbh RECOMBINATING CARBOXIPEPTIDASE B.
US20080159976A1 (en) * 2005-04-11 2008-07-03 Jacob Hartman Methods for lowering elevated uric acid levels using intravenous injections of PEG-uricase
US8148123B2 (en) 2005-04-11 2012-04-03 Savient Pharmaceuticals, Inc. Methods for lowering elevated uric acid levels using intravenous injections of PEG-uricase
WO2006110761A2 (en) 2005-04-11 2006-10-19 Savient Pharmaceuticals, Inc. A variant form of urate oxidase and use thereof
HUE052976T2 (en) 2005-04-11 2021-06-28 Horizon Pharma Rheumatology Llc Variant forms of urate oxidase and use thereof
WO2006125452A1 (en) * 2005-05-23 2006-11-30 Universite De Geneve Injectable superparamagnetic nanoparticles for treatment by hyperthermia and use for forming an hyperthermic implant
CN101622270B (en) 2006-04-12 2014-01-01 萨文特医药公司 Method for Purifying Proteins Using Cationic Surfactants
CN101058805B (en) * 2007-03-21 2011-09-07 北京贯虹科技有限公司 Method of producing mutation procarboxypeptidase B and mutation carboxypeptidase B
US8993714B2 (en) * 2007-10-26 2015-03-31 Imiplex Llc Streptavidin macromolecular adaptor and complexes thereof
US9102526B2 (en) 2008-08-12 2015-08-11 Imiplex Llc Node polypeptides for nanostructure assembly
WO2010132363A1 (en) 2009-05-11 2010-11-18 Imiplex Llc Method of protein nanostructure fabrication
HUP1200205A3 (en) 2009-06-25 2012-09-28 Savient Pharmaceuticals Method and kits for perdicting infusion reaction risk and antibody-mediated low of response by monitoring serum uric acid during pegylated uricare therapy
CN101967467B (en) * 2009-07-28 2012-11-14 上海雅心生物技术有限公司 Production and application of high-stability recombination carboxypeptidase B
CN110234340A (en) 2016-11-11 2019-09-13 好利恩风湿病制药有限责任公司 Combination therapy of prednisone and uricase molecules and uses thereof
US20200237881A1 (en) 2019-01-30 2020-07-30 Horizon Pharma Rheumatology Llc Reducing immunogenicity to pegloticase
US12121566B2 (en) 2019-01-30 2024-10-22 Horizon Therapeutics Usa, Inc. Methods for treating gout
US11918624B2 (en) * 2020-06-10 2024-03-05 Kelsius Laboratories LLC Therapeutic composition for use in the treatment of COVID-19 and other cytokine storm associated disorders
US12269875B2 (en) 2023-08-03 2025-04-08 Jeff R. Peterson Gout flare prevention methods using IL-1BETA blockers
CN121022890A (en) * 2025-08-01 2025-11-28 合肥美诺生物科技有限公司 Carboxypeptidase B precursor gene sequence, recombinant carboxypeptidase B precursor, and preparation method of carboxypeptidase B.

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK124483B (en) * 1967-12-04 1972-10-23 Bayer Ag Method for purifying carboxypeptidase B.
US4511503A (en) * 1982-12-22 1985-04-16 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
HU190129B (en) * 1983-07-11 1986-08-28 Reanal Finomvegyszergyar,Hu Process for the isolation of carboxypeptidase b enzyme from mammal pancreas
US5206161A (en) * 1991-02-01 1993-04-27 Genentech, Inc. Human plasma carboxypeptidase B
FR2692907B1 (en) * 1992-06-25 1995-06-30 Rhone Poulenc Rorer Sa MODIFIED KLUYVEROMYCES YEASTS, PREPARATION AND USE.
JP3472587B2 (en) * 1992-08-28 2003-12-02 アベンティス ファーマ株式会社 Bone-related carboxypeptidase-like protein and method for producing the same
BR9408061A (en) * 1993-11-16 1996-12-24 Lilly Co Eli DNA sequence encoding swine pancreatic carboxypeptidase b

Also Published As

Publication number Publication date
EP0871718A4 (en) 2000-06-07
WO1996023064A1 (en) 1996-08-01
IL116696A0 (en) 1996-05-14
BR9606795A (en) 1997-12-30
JP4250716B2 (en) 2009-04-08
EP0871718B1 (en) 2007-04-04
CN1177377A (en) 1998-03-25
DE69637011T2 (en) 2007-12-13
CN1144874C (en) 2004-04-07
EP0871718A1 (en) 1998-10-21
ATE358717T1 (en) 2007-04-15
DE69637011D1 (en) 2007-05-16
PL183228B1 (en) 2002-06-28
CA2210242C (en) 2010-04-27
CZ292541B6 (en) 2003-10-15
HUP9800091A2 (en) 1998-05-28
PT871718E (en) 2007-06-26
DK0871718T3 (en) 2007-07-02
KR19980701652A (en) 1998-06-25
ES2284167T3 (en) 2007-11-01
CA2210242A1 (en) 1996-08-01
HUP9800091A3 (en) 2004-03-29
AU4903496A (en) 1996-08-14
IL116696A (en) 1999-08-17
ZA96515B (en) 1996-08-15
US5948668A (en) 1999-09-07
CZ225897A3 (en) 1998-10-14
KR100566136B1 (en) 2006-11-10
NZ302874A (en) 1998-11-25
HK1014986A1 (en) 1999-10-08
MX9705279A (en) 1998-06-30
HU225673B1 (en) 2007-06-28
AU698889B2 (en) 1998-11-12
JPH11503002A (en) 1999-03-23

Similar Documents

Publication Publication Date Title
IL116696A0 (en) Production of enzymatically active recombinant carboxypeptidase b
ZA975573B (en) Method for producing xylitol.
FR11C0022I1 (en)
DE60027765D1 (en) METHOD FOR PRODUCING MECHANICAL POWDER
AU1155495A (en) Process for the preparation of non-cariogenic sugars
EP0869174A3 (en) Method for cloning and producing the SpeI restriction endonuclease
MX9605272A (en) Process to prepare nicotinamide.
GB9424567D0 (en) Process for the production of xylitol
MY119738A (en) Process for the purification of an alkanolamine
AU5681398A (en) Novel process for decomposing siloxane bond-containing compound
WO1999058691A3 (en) Recombinant (alpha)-l-iduronidase, methods for producing and purifying the same and methods for treating diseases caused by deficiencies thereof
AU1941597A (en) Protein having cellulase activities and process for producing the same
SE9502077L (en) Methods of producing chlorine dioxide
WO1993018166A3 (en) PRODUCTION OF $i(ASPERGILLUS NIGER CATALASE-R)
EP0733711A3 (en) Method for cloning and producing the SacI restriction endonuclease
HUT47976A (en) Process for producing recombinant expression dna vectors encoding isopenicillin-n-synthetase enzyme of aspergillus nidulans, as well as dna molecules
ZA961424B (en) Method for production of carbon electrodes.
TW375603B (en) Separation of 2-aminomethyl cyclopentylamine from a mixture comprising hexamethylenediamine and 2-aminomethyl cyclopentylamine
EP0959131A3 (en) Method for cloning and producing the AgeI restriction endonuclease in E. coli
EP0324248A3 (en) Method for producing the fnudi restriction endonuclease and methylase
EP0332406A3 (en) Method for producing the ase i restriction endonuclease and methylase
EP0355981A3 (en) Method for producing the n1aiv restriction endonuclease and methylase
EP0780398A3 (en) Process for purifying tammhorsfall glycoprotein or uromodulin, their purified products and method for making the discrimination between both of them
MX9603672A (en) Process for producing a cyclosporine.
WO2001019989A3 (en) Method of producing and purifying endostatintm protein