PL3216867T3 - Sposób modyfikowania sekwencji genomu w celu wprowadzania swoistej mutacji do docelowej sekwencji DNA poprzez reakcję usuwania zasady i stosowany w nim kompleks cząsteczkowy - Google Patents

Sposób modyfikowania sekwencji genomu w celu wprowadzania swoistej mutacji do docelowej sekwencji DNA poprzez reakcję usuwania zasady i stosowany w nim kompleks cząsteczkowy

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Publication number
PL3216867T3
PL3216867T3 PL15857325T PL15857325T PL3216867T3 PL 3216867 T3 PL3216867 T3 PL 3216867T3 PL 15857325 T PL15857325 T PL 15857325T PL 15857325 T PL15857325 T PL 15857325T PL 3216867 T3 PL3216867 T3 PL 3216867T3
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Poland
Prior art keywords
base
molecular complex
specific mutation
complex used
removal reaction
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PL15857325T
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English (en)
Inventor
Keiji Nishida
Akihiko Kondo
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National University Corporation Kobe University
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Application filed by National University Corporation Kobe University filed Critical National University Corporation Kobe University
Publication of PL3216867T3 publication Critical patent/PL3216867T3/pl

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1024—In vivo mutagenesis using high mutation rate "mutator" host strains by inserting genetic material, e.g. encoding an error prone polymerase, disrupting a gene for mismatch repair
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
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    • C07—ORGANIC CHEMISTRY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C12N9/222—Clustered regularly interspaced short palindromic repeats [CRISPR]-associated [CAS] enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2497—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing N- glycosyl compounds (3.2.2)
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    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/02—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2) hydrolysing N-glycosyl compounds (3.2.2)
    • C12Y302/02027—Uracil-DNA glycosylase (3.2.2.27)
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    • C12N2310/00—Structure or type of the nucleic acid
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]

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  • Life Sciences & Earth Sciences (AREA)
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  • Engineering & Computer Science (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
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  • General Engineering & Computer Science (AREA)
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  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
PL15857325T 2014-11-04 2015-11-02 Sposób modyfikowania sekwencji genomu w celu wprowadzania swoistej mutacji do docelowej sekwencji DNA poprzez reakcję usuwania zasady i stosowany w nim kompleks cząsteczkowy PL3216867T3 (pl)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2014224745 2014-11-04
EP15857325.3A EP3216867B1 (en) 2014-11-04 2015-11-02 Method for modifying genome sequence to introduce specific mutation to targeted dna sequence by base-removal reaction, and molecular complex used therein
PCT/JP2015/080958 WO2016072399A1 (ja) 2014-11-04 2015-11-02 脱塩基反応により標的化したｄｎａ配列に特異的に変異を導入する、ゲノム配列の改変方法、並びにそれに用いる分子複合体

Publications (1)

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PL3216867T3 true PL3216867T3 (pl) 2020-11-02

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PL15857325T PL3216867T3 (pl) 2014-11-04 2015-11-02 Sposób modyfikowania sekwencji genomu w celu wprowadzania swoistej mutacji do docelowej sekwencji DNA poprzez reakcję usuwania zasady i stosowany w nim kompleks cząsteczkowy

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US (2) US10920215B2 (pl)
EP (2) EP3739047A1 (pl)
JP (1) JP6153180B2 (pl)
DK (1) DK3216867T3 (pl)
ES (1) ES2800168T3 (pl)
PL (1) PL3216867T3 (pl)
PT (1) PT3216867T (pl)
WO (1) WO2016072399A1 (pl)

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US20170321210A1 (en) 2017-11-09
DK3216867T3 (da) 2020-06-22
US10920215B2 (en) 2021-02-16
US20210171935A1 (en) 2021-06-10
ES2800168T3 (es) 2020-12-28
JPWO2016072399A1 (ja) 2017-04-27
PT3216867T (pt) 2020-07-16
EP3216867A1 (en) 2017-09-13
WO2016072399A1 (ja) 2016-05-12
JP6153180B2 (ja) 2017-06-28
EP3216867A4 (en) 2018-04-04
EP3216867B1 (en) 2020-04-15
EP3739047A1 (en) 2020-11-18

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