PL3218514T3 - Przewidywanie genetycznie stabilnej produkcji rekombinowanego białka we wczesnym rozwoju linii komórkowej - Google Patents

Przewidywanie genetycznie stabilnej produkcji rekombinowanego białka we wczesnym rozwoju linii komórkowej

Info

Publication number
PL3218514T3
PL3218514T3 PL15797902T PL15797902T PL3218514T3 PL 3218514 T3 PL3218514 T3 PL 3218514T3 PL 15797902 T PL15797902 T PL 15797902T PL 15797902 T PL15797902 T PL 15797902T PL 3218514 T3 PL3218514 T3 PL 3218514T3
Authority
PL
Poland
Prior art keywords
predicting
cell line
recombinant protein
protein production
genetically stable
Prior art date
Application number
PL15797902T
Other languages
English (en)
Inventor
Uros JAMNIKAR
Kristina Gruden
Original Assignee
Lek Pharmaceuticals D.D.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lek Pharmaceuticals D.D. filed Critical Lek Pharmaceuticals D.D.
Publication of PL3218514T3 publication Critical patent/PL3218514T3/pl

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0603Embryonic cells ; Embryoid bodies
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5023Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on expression patterns
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00Genetically modified cells
    • C12N2510/02Cells for production
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/158Expression markers
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/71Assays involving receptors, cell surface antigens or cell surface determinants for growth factors; for growth regulators
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90Enzymes; Proenzymes
    • G01N2333/902Oxidoreductases (1.)
    • G01N2333/906Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.7)
    • G01N2333/9065Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.7) acting on CH-NH groups of donors (1.5)
    • G01N2333/90655Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.7) acting on CH-NH groups of donors (1.5) with NAD or NADP as acceptor (1.5.1) in general
    • G01N2333/90661Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.7) acting on CH-NH groups of donors (1.5) with NAD or NADP as acceptor (1.5.1) in general with a definite EC number (1.5.1.-)
    • G01N2333/90666Dihydrofolate reductase [DHFR] (1.5.1.3)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biophysics (AREA)
  • Reproductive Health (AREA)
  • Gynecology & Obstetrics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Toxicology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
PL15797902T 2014-11-12 2015-11-12 Przewidywanie genetycznie stabilnej produkcji rekombinowanego białka we wczesnym rozwoju linii komórkowej PL3218514T3 (pl)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP14192809 2014-11-12
PCT/EP2015/076390 WO2016075216A1 (en) 2014-11-12 2015-11-12 Predicting genetically stable recombinant protein production in early cell line development
EP15797902.2A EP3218514B1 (en) 2014-11-12 2015-11-12 Predicting genetically stable recombinant protein production in early cell line development

Publications (1)

Publication Number Publication Date
PL3218514T3 true PL3218514T3 (pl) 2020-01-31

Family

ID=51947148

Family Applications (1)

Application Number Title Priority Date Filing Date
PL15797902T PL3218514T3 (pl) 2014-11-12 2015-11-12 Przewidywanie genetycznie stabilnej produkcji rekombinowanego białka we wczesnym rozwoju linii komórkowej

Country Status (6)

Country Link
US (1) US10913984B2 (pl)
EP (1) EP3218514B1 (pl)
DK (1) DK3218514T3 (pl)
PL (1) PL3218514T3 (pl)
SI (1) SI3218514T1 (pl)
WO (1) WO2016075216A1 (pl)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4582536A4 (en) * 2022-08-31 2025-12-03 Fujifilm Corp METHOD FOR PREDICTING THE PRODUCTION STABILITY OF A CLONE THAT PRODUCES A USEFUL SUBSTANCE, INFORMATION PROCESSING DEVICE, PROGRAM AND METHOD FOR GENERATION OF A PREDICTION MODEL

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6136536A (en) 1997-10-29 2000-10-24 Genetics Institute, Inc. Rapid generation of stable mammalian cell lines producing high levels of recombinant proteins
EP2412816B1 (en) * 2004-07-26 2014-12-03 Pfenex Inc. Process for improved protein expression by strain engineering
EP2423684A3 (en) 2006-04-21 2012-06-27 Wyeth LLC Differential expression profiling analysis of cell culture phenotypes and the uses thereof
WO2008024459A2 (en) 2006-08-24 2008-02-28 Govt. Of The Usa , As Represented By The Sec., Dept. Of Health And Human Services Compositions and methods for modifying cellular properties
WO2008157299A2 (en) 2007-06-15 2008-12-24 Wyeth Differential expression profiling analysis of cell culture phenotypes and uses thereof
GB0813253D0 (en) 2008-07-18 2008-08-27 Univ Aston Protein expression system
CN102414320A (zh) 2009-05-15 2012-04-11 贝林格尔.英格海姆国际有限公司 具有降低的nocr表达的改良细胞系及其用途
EP2453007A1 (en) * 2010-11-10 2012-05-16 LEK Pharmaceuticals d.d. Nuclear DNA content of CHO cell lines as a selection criterion in screening of the best recombinant protein producers
WO2012102793A2 (en) * 2010-12-10 2012-08-02 Zirus, Inc. Mammalian genes involved in toxicity and infection

Also Published As

Publication number Publication date
SI3218514T1 (sl) 2019-11-29
EP3218514B1 (en) 2019-07-24
US10913984B2 (en) 2021-02-09
EP3218514A1 (en) 2017-09-20
US20170314082A1 (en) 2017-11-02
WO2016075216A1 (en) 2016-05-19
DK3218514T3 (da) 2019-10-21

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