PL323475A1 - Large-scale paragene purification process - Google Patents

Large-scale paragene purification process

Info

Publication number
PL323475A1
PL323475A1 PL96323475A PL32347596A PL323475A1 PL 323475 A1 PL323475 A1 PL 323475A1 PL 96323475 A PL96323475 A PL 96323475A PL 32347596 A PL32347596 A PL 32347596A PL 323475 A1 PL323475 A1 PL 323475A1
Authority
PL
Poland
Prior art keywords
paragene
scale
purification process
purification
scale paragene
Prior art date
Application number
PL96323475A
Inventor
Ann L Lee
Sangeetha Sagar
Original Assignee
Merck & Co Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck & Co Inc filed Critical Merck & Co Inc
Publication of PL323475A1 publication Critical patent/PL323475A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
PL96323475A 1995-05-19 1996-05-15 Large-scale paragene purification process PL323475A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US44611895A 1995-05-19 1995-05-19
PCT/US1996/007083 WO1996036706A1 (en) 1995-05-19 1996-05-15 A method for large scale plasmid purification

Publications (1)

Publication Number Publication Date
PL323475A1 true PL323475A1 (en) 1998-03-30

Family

ID=23771380

Family Applications (1)

Application Number Title Priority Date Filing Date
PL96323475A PL323475A1 (en) 1995-05-19 1996-05-15 Large-scale paragene purification process

Country Status (18)

Country Link
EP (1) EP0827536A1 (en)
JP (1) JPH11505707A (en)
KR (1) KR19990014924A (en)
CN (1) CN1190435A (en)
AR (1) AR003122A1 (en)
AU (1) AU709003B2 (en)
CA (1) CA2220867A1 (en)
CZ (1) CZ366197A3 (en)
EA (1) EA000785B1 (en)
HR (1) HRP960222A2 (en)
HU (1) HUP9802557A3 (en)
MX (1) MX9708967A (en)
NO (1) NO975280L (en)
NZ (1) NZ309231A (en)
PL (1) PL323475A1 (en)
SK (1) SK155797A3 (en)
WO (1) WO1996036706A1 (en)
YU (1) YU29796A (en)

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6197553B1 (en) 1994-07-15 2001-03-06 Merck & Co., Inc. Method for large scale plasmid purification
AU708798B2 (en) * 1994-07-15 1999-08-12 Merck & Co., Inc. A method for large scale plasmid purification
GB9425138D0 (en) 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
US5981735A (en) * 1996-02-12 1999-11-09 Cobra Therapeutics Limited Method of plasmid DNA production and purification
FR2753204B1 (en) * 1996-09-11 1998-12-04 Transgene Sa PROCESS FOR THE PREPARATION OF PLASMID DNA
SE9703532D0 (en) * 1997-09-30 1997-09-30 Pharmacia Biotech Ab A process for the purification of plasmid DNA
US6914137B2 (en) 1997-12-06 2005-07-05 Dna Research Innovations Limited Isolation of nucleic acids
WO1999063076A1 (en) * 1998-06-01 1999-12-09 The Immune Response Corporation Novel method of large scale plasmid purification
US6310199B1 (en) 1999-05-14 2001-10-30 Promega Corporation pH dependent ion exchange matrix and method of use in the isolation of nucleic acids
US6270970B1 (en) * 1999-05-14 2001-08-07 Promega Corporation Mixed-bed solid phase and its use in the isolation of nucleic acids
US20020012990A1 (en) 1999-12-22 2002-01-31 Lander Russel Jackson Process for the scaleable purification of plasmid DNA
ES2251441T3 (en) * 2001-01-19 2006-05-01 Drm, Dr. Muller Ag INTEGRATED PREPARATION OF BIOMASS FROM A CELL CULTURE PROCESS FOR THE MANUFACTURE OF CLEAR CELL LISATE, CONTAINING PLASMID DNA.
GB0107634D0 (en) 2001-03-27 2001-05-16 Fermentas Ab Nucleic acid purification
US6406892B1 (en) 2001-05-23 2002-06-18 Bio-Rad Laboratories, Inc. Acetate-free purification of plasmid DNA on hydroxyapatite
SE0200543D0 (en) * 2002-02-21 2002-02-21 Amersham Biosciences Ab Method of separation using aromatic thioether ligands
US8501402B2 (en) 2003-03-24 2013-08-06 Boehringer Ingelheim Rcv Gmbh & Co Kg Methods and devices for producing biomolecules
JP4971997B2 (en) 2005-01-31 2012-07-11 メルク・シャープ・エンド・ドーム・コーポレイション Purification method of plasmid DNA
JP2006238822A (en) * 2005-03-04 2006-09-14 Fuji Photo Film Co Ltd Method for separating and purifying nucleic acid
DE102005059315A1 (en) * 2005-12-09 2007-06-14 Qiagen Gmbh Process for the enrichment of short-chain nucleic acids
CN100439497C (en) * 2006-05-26 2008-12-03 吉林大学 A purification method suitable for large-scale production of plasmid DNA
WO2008045505A2 (en) 2006-10-10 2008-04-17 Xenomics, Inc. Compositions, methods and kits for isolating nucleic acids from body fluids using anion exchange media
US20090004716A1 (en) * 2007-05-23 2009-01-01 Vgx Pharmaceuticlas, Inc. Compositions comprising high concentration of biologically active molecules and processes for preparing the same
CN101070543B (en) * 2007-06-01 2010-12-29 广东药学院 Supercoiled plasmid isolation and purification process
GB0913160D0 (en) * 2009-07-29 2009-09-02 Avecia Biolog Ltd Extraction process
JP2013528786A (en) 2010-04-08 2013-07-11 キアゲン ゲーエムベーハー Chromatographic device and method for isolating and purifying nucleic acids
WO2011124705A1 (en) * 2010-04-08 2011-10-13 Qiagen Gmbh Method for isolating and purifying nucleic acids
JP5624487B2 (en) 2011-01-31 2014-11-12 横河電機株式会社 Nucleic acid extraction method
JP6147730B2 (en) 2011-04-27 2017-06-14 メルク パテント ゲゼルシャフト ミット ベシュレンクテル ハフツングＭｅｒｃｋ Ｐａｔｅｎｔ Ｇｅｓｅｌｌｓｃｈａｆｔ ｍｉｔ ｂｅｓｃｈｒａｅｎｋｔｅｒ Ｈａｆｔｕｎｇ How to lyse cells
CN104099321A (en) * 2014-07-10 2014-10-15 广州市军科泰特医药科技有限公司 Preparation method of lung cancer resistant plasmid T-VISA (VP16-Gal4-WPRE integrated systemic amplifier)-Bik (bcl-2interacting killer) DD
CN106884011B (en) * 2015-12-16 2020-11-17 固安鼎泰海规生物科技有限公司 Combined liquid chromatography separation method for large-scale plasmid purification
CN111721871A (en) * 2020-06-24 2020-09-29 南京济群生物科技有限公司 High-resolution detection method for plasmid supercoiled DNA content
CN112798697B (en) * 2020-11-16 2022-11-22 武汉波睿达生物科技有限公司 Detection method for supercoiled structure purity of lentivirus packaging system helper plasmid
CN114456940B (en) * 2022-01-23 2024-05-03 和元生物技术（上海）股份有限公司 Method for improving plasmid stability in escherichia coli cracking process
CN120882735A (en) 2023-01-25 2025-10-31 诺韦尔生物技术美国公司 Expression system for product manufacturing

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0452457B1 (en) * 1989-11-03 1997-08-20 Vanderbilt University Method of in vivo delivery of functioning foreign genes
WO1993024640A2 (en) * 1992-06-04 1993-12-09 The Regents Of The University Of California Methods and compositions for in vivo gene therapy
US5834441A (en) * 1993-09-13 1998-11-10 Rhone-Poulenc Rorer Pharmaceuticals Inc. Adeno-associated viral (AAV) liposomes and methods related thereto
AU708798B2 (en) * 1994-07-15 1999-08-12 Merck & Co., Inc. A method for large scale plasmid purification

Also Published As

Publication number Publication date
WO1996036706A1 (en) 1996-11-21
JPH11505707A (en) 1999-05-25
AU709003B2 (en) 1999-08-19
CZ366197A3 (en) 1998-04-15
HRP960222A2 (en) 1997-12-31
AR003122A1 (en) 1998-07-08
EA000785B1 (en) 2000-04-24
NO975280D0 (en) 1997-11-18
NO975280L (en) 1998-01-16
EA199700399A1 (en) 1998-06-25
AU5921996A (en) 1996-11-29
KR19990014924A (en) 1999-02-25
HUP9802557A2 (en) 1999-02-01
YU29796A (en) 1999-06-15
SK155797A3 (en) 1998-07-08
MX9708967A (en) 1998-03-31
HUP9802557A3 (en) 2000-04-28
CN1190435A (en) 1998-08-12
NZ309231A (en) 1999-10-28
EP0827536A1 (en) 1998-03-11
CA2220867A1 (en) 1996-11-21

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