PL3523420T3 - Kompozycje, urządzenia i sposoby testowania in vitro mikrośrodowiska chemicznego w hodowlach komórkowych - Google Patents
Kompozycje, urządzenia i sposoby testowania in vitro mikrośrodowiska chemicznego w hodowlach komórkowychInfo
- Publication number
- PL3523420T3 PL3523420T3 PL17791165.8T PL17791165T PL3523420T3 PL 3523420 T3 PL3523420 T3 PL 3523420T3 PL 17791165 T PL17791165 T PL 17791165T PL 3523420 T3 PL3523420 T3 PL 3523420T3
- Authority
- PL
- Poland
- Prior art keywords
- compositions
- methods
- devices
- cell cultures
- vitro testing
- Prior art date
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
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- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/04—Enzymes or microbial cells immobilised on or in an organic carrier entrapped within the carrier, e.g. gel or hollow fibres
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- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/06—Enzymes or microbial cells immobilised on or in an organic carrier attached to the carrier via a bridging agent
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- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
- C12N11/082—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
- C12N11/087—Acrylic polymers
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- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
- C12N11/089—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
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- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
- C12N11/12—Cellulose or derivatives thereof
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0068—General culture methods using substrates
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0693—Tumour cells; Cancer cells
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
- C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0053—Oxidoreductases (1.) acting on a heme group of donors (1.9)
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0055—Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10)
- C12N9/0057—Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10) with oxygen as acceptor (1.10.3)
- C12N9/0061—Laccase (1.10.3.2)
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0065—Oxidoreductases (1.) acting on hydrogen peroxide as acceptor (1.11)
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0093—Oxidoreductases (1.) acting on CH or CH2 groups (1.17)
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
- C12Y101/03—Oxidoreductases acting on the CH-OH group of donors (1.1) with a oxygen as acceptor (1.1.3)
- C12Y101/03004—Glucose oxidase (1.1.3.4)
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- C12Y—ENZYMES
- C12Y106/00—Oxidoreductases acting on NADH or NADPH (1.6)
- C12Y106/03—Oxidoreductases acting on NADH or NADPH (1.6) with oxygen as acceptor (1.6.3)
- C12Y106/03001—NAD(P)H oxidase (1.6.3.1), i.e. NOX1
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- C12Y109/00—Oxidoreductases acting on a heme group of donors (1.9)
- C12Y109/03—Oxidoreductases acting on a heme group of donors (1.9) with oxygen as acceptor (1.9.3)
- C12Y109/03001—Cytochrome-c oxidase (1.9.3.1)
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- C12Y110/00—Oxidoreductases acting on diphenols and related substances as donors (1.10)
- C12Y110/03—Oxidoreductases acting on diphenols and related substances as donors (1.10) with an oxygen as acceptor (1.10.3)
- C12Y110/03002—Laccase (1.10.3.2)
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- C12Y—ENZYMES
- C12Y111/00—Oxidoreductases acting on a peroxide as acceptor (1.11)
- C12Y111/01—Peroxidases (1.11.1)
- C12Y111/01006—Catalase (1.11.1.6)
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- C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
- C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
- C12Y113/12004—Lactate 2-monooxygenase (1.13.12.4)
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- C12Y117/00—Oxidoreductases acting on CH or CH2 groups (1.17)
- C12Y117/03—Oxidoreductases acting on CH or CH2 groups (1.17) with oxygen as acceptor (1.17.3)
- C12Y117/03002—Xanthine oxidase (1.17.3.2)
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
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- C12N2500/00—Specific components of cell culture medium
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/70—Enzymes
- C12N2501/71—Oxidoreductases (EC 1.)
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Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IT102016000099380A IT201600099380A1 (it) | 2016-10-04 | 2016-10-04 | Composizioni, dispositivi e metodi per il controllo in vitro del microambiente chimico in colture cellulari |
| PCT/IB2017/056081 WO2018065887A1 (en) | 2016-10-04 | 2017-10-03 | Compositions, devices and methods for the control in vitro of chemical microambient in cell cultures |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| PL3523420T3 true PL3523420T3 (pl) | 2026-04-13 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PL17791165.8T PL3523420T3 (pl) | 2016-10-04 | 2017-10-03 | Kompozycje, urządzenia i sposoby testowania in vitro mikrośrodowiska chemicznego w hodowlach komórkowych |
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| Country | Link |
|---|---|
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| IT (1) | IT201600099380A1 (pl) |
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| IT201600099380A1 (it) | 2016-10-04 | 2018-04-04 | Univ Bologna Alma Mater Studiorum | Composizioni, dispositivi e metodi per il controllo in vitro del microambiente chimico in colture cellulari |
| CN108753909B (zh) * | 2018-06-07 | 2022-04-05 | 苏州良辰生物医药科技有限公司 | 一种抑制葡萄糖氧化酶对细胞毒性的方法 |
| CN110478525B (zh) * | 2019-07-26 | 2021-12-07 | 广东省医疗器械研究所 | 一种糖与酶协同作用的自交联水凝胶及其制备方法 |
| CN112410281A (zh) * | 2020-11-18 | 2021-02-26 | 北京大学 | 一种柔性基底的细胞细菌互作模型的制备方法及应用 |
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| JP2012239444A (ja) * | 2011-05-23 | 2012-12-10 | Asahi Glass Co Ltd | 細胞培養担体、細胞培養担体の製造方法、及び細胞培養方法 |
| WO2013134401A2 (en) * | 2012-03-06 | 2013-09-12 | Northeastern University | Enzymatic nanosensor compositions and methods |
| SG11201500188YA (en) * | 2012-08-08 | 2015-02-27 | Univ Nanyang Tech | Methods of manufacturing hydrogel microparticles having living cells, and compositions for manufacturing a scaffold for tissue engineering |
| WO2014065863A1 (en) * | 2012-10-22 | 2014-05-01 | The Regents Of The University Of California | Compositions and methods for promoting collagen-crosslinking |
| WO2014089472A1 (en) * | 2012-12-07 | 2014-06-12 | Kansas State University Research Foundation | Peptide-albumin hydrogel properties and its applications |
| EP2796873A1 (en) * | 2013-04-25 | 2014-10-29 | QGel SA | Method for a cell-based drug screening assay and the use thereof |
| EP2801613A1 (en) * | 2013-05-08 | 2014-11-12 | Ecole Polytechnique Fédérale de Lausanne (EPFL) | Arrays of discrete cell culture microenvironments, methods of making such arrays and uses thereof |
| US20150196584A1 (en) * | 2013-11-08 | 2015-07-16 | The Johns Hopkins University | Oxygen-controllable and hypoxia-inducible hydrogels |
| GB201421092D0 (en) | 2014-11-27 | 2015-01-14 | Koninklijke Nederlandse Akademie Van Wetenschappen | Culture medium |
| IT201600099380A1 (it) | 2016-10-04 | 2018-04-04 | Univ Bologna Alma Mater Studiorum | Composizioni, dispositivi e metodi per il controllo in vitro del microambiente chimico in colture cellulari |
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2016
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| US20240254436A1 (en) | 2024-08-01 |
| CA3039282A1 (en) | 2018-04-12 |
| EP3523420A1 (en) | 2019-08-14 |
| JP2019528795A (ja) | 2019-10-17 |
| JP7553930B2 (ja) | 2024-09-19 |
| WO2018065887A1 (en) | 2018-04-12 |
| EP3523420B1 (en) | 2025-11-26 |
| IT201600099380A1 (it) | 2018-04-04 |
| US20190300846A1 (en) | 2019-10-03 |
| EP3523420C0 (en) | 2025-11-26 |
| US11920153B2 (en) | 2024-03-05 |
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