PL353634A1 - Methods and compositions for directed cloning and subcloning using homologous recombination - Google Patents

Methods and compositions for directed cloning and subcloning using homologous recombination

Info

Publication number
PL353634A1
PL353634A1 PL00353634A PL35363400A PL353634A1 PL 353634 A1 PL353634 A1 PL 353634A1 PL 00353634 A PL00353634 A PL 00353634A PL 35363400 A PL35363400 A PL 35363400A PL 353634 A1 PL353634 A1 PL 353634A1
Authority
PL
Poland
Prior art keywords
compositions
methods
homologous recombination
subcloning
cloning
Prior art date
Application number
PL00353634A
Other languages
English (en)
Polish (pl)
Inventor
A.FrancisStewert A.Francis Stewert
YoumingZhang Youming Zhang
Joep Pieter PaulMuyrers Joep Pieter Paul Muyrers
Original Assignee
The European Molecular Biology Laboratorythe European Molecular Biology Laboratory
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The European Molecular Biology Laboratorythe European Molecular Biology Laboratory filed Critical The European Molecular Biology Laboratorythe European Molecular Biology Laboratory
Publication of PL353634A1 publication Critical patent/PL353634A1/xx

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Production Of Liquid Hydrocarbon Mixture For Refining Petroleum (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Solid Fuels And Fuel-Associated Substances (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Detergent Compositions (AREA)
  • Medicinal Preparation (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Nitrogen Condensed Heterocyclic Rings (AREA)
PL00353634A 1999-07-09 2000-07-10 Methods and compositions for directed cloning and subcloning using homologous recombination PL353634A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/350,830 US6355412B1 (en) 1999-07-09 1999-07-09 Methods and compositions for directed cloning and subcloning using homologous recombination
PCT/EP2000/006533 WO2001004288A1 (en) 1999-07-09 2000-07-10 Methods and compositions for directed cloning and subcloning using homologous recombination

Publications (1)

Publication Number Publication Date
PL353634A1 true PL353634A1 (en) 2003-12-01

Family

ID=23378374

Family Applications (1)

Application Number Title Priority Date Filing Date
PL00353634A PL353634A1 (en) 1999-07-09 2000-07-10 Methods and compositions for directed cloning and subcloning using homologous recombination

Country Status (20)

Country Link
US (1) US6355412B1 (ja)
EP (1) EP1204740B1 (ja)
JP (2) JP4312981B2 (ja)
KR (1) KR100751587B1 (ja)
CN (2) CN100469877C (ja)
AT (1) ATE305035T1 (ja)
AU (1) AU781379B2 (ja)
BR (1) BR0012283A (ja)
CA (1) CA2377938C (ja)
DE (1) DE60022766T2 (ja)
DK (1) DK1204740T3 (ja)
ES (1) ES2249291T3 (ja)
HU (1) HU228977B1 (ja)
IL (1) IL147385A0 (ja)
MX (1) MX252928B (ja)
NZ (1) NZ516722A (ja)
PL (1) PL353634A1 (ja)
WO (1) WO2001004288A1 (ja)
YU (1) YU1502A (ja)
ZA (1) ZA200200152B (ja)

Families Citing this family (87)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1025217B1 (en) * 1997-10-24 2006-10-04 Invitrogen Corporation Recombinational cloning using nucleic acids having recombination sites
US6355412B1 (en) * 1999-07-09 2002-03-12 The European Molecular Biology Laboratory Methods and compositions for directed cloning and subcloning using homologous recombination
DK1311661T3 (da) * 2000-08-14 2012-11-26 Us Gov Health & Human Serv Forøget homolog rekombination medieret ved lambda-rekombinationsproteiner
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US6596541B2 (en) 2000-10-31 2003-07-22 Regeneron Pharmaceuticals, Inc. Methods of modifying eukaryotic cells
US20050144655A1 (en) 2000-10-31 2005-06-30 Economides Aris N. Methods of modifying eukaryotic cells
US7083976B2 (en) * 2001-06-30 2006-08-01 The European Molecular Biology Laboratory Tyrosine recombinase for genetic engineering
EP1409702A1 (en) * 2001-07-20 2004-04-21 Youming Zhang Improved rect or recet cloning and subcloning method
US7338800B2 (en) * 2002-01-16 2008-03-04 Baylor College Of Medicine In vivo gene transfer
CA2473741C (en) * 2002-01-18 2015-12-22 Morphotek, Inc. A method for generating engineered cells for locus specific gene regulation and analysis
WO2003089587A2 (en) * 2002-04-16 2003-10-30 Promega Corporation Method to enhance homologous recombination
US20040106566A1 (en) * 2002-05-17 2004-06-03 Shi-Lung Lin RNA-splicing and processing-directed gene silencing and the relative applications thereof
AU2003257155A1 (en) * 2002-08-02 2004-02-23 The General Hospital Corporation Methods for the production of cells and mammals with desired genetic modifications
US7135324B2 (en) * 2002-09-04 2006-11-14 The University Of Connecticut Viral recombinases, related articles, and methods of use thereof
US7563600B2 (en) * 2002-09-12 2009-07-21 Combimatrix Corporation Microarray synthesis and assembly of gene-length polynucleotides
AU2003289195A1 (en) * 2002-12-10 2004-06-30 Kazusa Dna Research Institute Foundation METHOD OF EFFICIENTLY RECOMBINING ORF ENCODED BY cDNA AND TEMPLATE VECTOR, TRAP VECTOR AND PRIMER TO BE USED THEREIN
US20040209370A1 (en) * 2002-12-19 2004-10-21 Wonchul Suh Method for chromosomal engineering
WO2005017128A2 (en) * 2003-02-26 2005-02-24 University Of Massachusetts Improved reagents for recombinogenic engineering and uses thereof
US7521242B2 (en) * 2003-05-09 2009-04-21 The United States Of America As Represented By The Department Of Health And Human Services Host cells deficient for mismatch repair and their use in methods for inducing homologous recombination using single-stranded nucleic acids
US20100069614A1 (en) 2008-06-27 2010-03-18 Merus B.V. Antibody producing non-human mammals
WO2004106375A1 (en) 2003-05-30 2004-12-09 Merus Biopharmaceuticals B.V. I.O. Fab library for the preparation of anti vegf and anti rabies virus fabs
US7781190B2 (en) * 2003-07-24 2010-08-24 The University Of Hong Kong Method for constructing and modifying large DNA molecules
WO2005014796A2 (en) 2003-08-08 2005-02-17 Invitrogen Corporation Methods and compositions for seamless cloning of nucleic acid molecules
WO2005054438A2 (en) * 2003-12-01 2005-06-16 Invitrogen Corporation Nucleic acid molecules containing recombination sites and methods of using the same
AU2005222788A1 (en) * 2004-02-27 2005-09-29 President And Fellows Of Harvard College Polynucleotide synthesis
US7674621B2 (en) 2004-05-21 2010-03-09 The United States Of America As Represented By The Department Of Health And Human Services Plasmids and phages for homologous recombination and methods of use
GB0423755D0 (en) * 2004-10-26 2004-11-24 Gene Bridges Gmbh Methods for heterologus expression of secondary metabolites
US20080051326A1 (en) * 2004-11-12 2008-02-28 Alexander Dylan C Antiinfective Lipopeptides
WO2006127423A2 (en) * 2005-05-18 2006-11-30 Codon Devices, Inc. Methods of producing polynucleotide libraries using scarless ligation
WO2007005053A1 (en) * 2005-06-30 2007-01-11 Codon Devices, Inc. Hierarchical assembly methods for genome engineering
WO2007136834A2 (en) * 2006-05-19 2007-11-29 Codon Devices, Inc. Combined extension and ligation for nucleic acid assembly
US8053191B2 (en) 2006-08-31 2011-11-08 Westend Asset Clearinghouse Company, Llc Iterative nucleic acid assembly using activation of vector-encoded traits
EP2086180B1 (en) * 2006-12-07 2014-05-28 Huawei Technologies Co., Ltd. Method and filtration server for filtering communication content of roaming user
CN101016551B (zh) * 2007-02-01 2010-05-19 南京师范大学 一种在dna载体中同时引入多个dna片段的方法
WO2008142028A1 (en) 2007-05-17 2008-11-27 Boehringer Ingelheim Rcv Gmbh & Co Kg Method for producing a recombinant protein on a manufacturing scale
GB0803109D0 (en) * 2008-02-20 2008-03-26 Gene Bridges Gmbh Method of nucleic acid recombination
EP2251423B1 (en) * 2008-03-07 2016-01-13 National University Corporation University Of Toyama Homologous recombination method, cloning method, and kit
DK3622815T3 (da) 2009-07-08 2023-06-26 Kymab Ltd Gnavermodeller og terapeutiske molekyler
WO2011053957A2 (en) 2009-11-02 2011-05-05 Gen9, Inc. Compositions and methods for the regulation of multiple genes of interest in a cell
US20110125118A1 (en) * 2009-11-20 2011-05-26 Opx Biotechnologies, Inc. Production of an Organic Acid and/or Related Chemicals
GB0922108D0 (en) 2009-12-17 2010-02-03 Gene Bridges Gmbh Heterologous hosts
WO2011080505A2 (en) 2009-12-28 2011-07-07 Bigdna Ltd Method of growing phage for commercial use
US9096825B2 (en) 2010-05-27 2015-08-04 Pharmazell Gmbh 7 α-hydroxysteroid dehydrogenase knockout mutants and use therefor
GB201009732D0 (en) 2010-06-10 2010-07-21 Gene Bridges Gmbh Direct cloning
CN103228130B (zh) 2010-06-17 2016-03-16 科马布有限公司 动物模型及治疗分子
CN103502448B (zh) 2010-11-12 2017-03-29 Gen9股份有限公司 核酸合成的方法和设备
WO2012064975A1 (en) 2010-11-12 2012-05-18 Gen9, Inc. Protein arrays and methods of using and making the same
CA2868113A1 (en) 2011-03-22 2012-09-27 Opx Biotechnologies, Inc. Microbial production of chemical products and related compositions, methods and systems
US9920323B2 (en) 2011-07-07 2018-03-20 Delphi Genetics Genetically modified phage and use thereof
EP2543720A1 (en) 2011-07-07 2013-01-09 Delphi Genetics Genetically modified phage and use thereof
EP2729563B1 (en) 2011-07-07 2018-09-12 Delphi Genetics Genetically modified phage and use thereof
LT2944693T (lt) 2011-08-26 2019-08-26 Gen9, Inc. Kompozicijos ir būdai, skirti nukleorūgščių didelio tikslumo sąrankai
BR112014006394A2 (pt) 2011-09-19 2017-03-28 Kymab Ltd manipulação de diversidade genética de imunoglobulina e terapêuticos multi-anticorpos
WO2014153188A2 (en) 2013-03-14 2014-09-25 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
CN107058059B (zh) 2011-09-26 2020-08-07 基因技术股份公司 高效的小体积核酸合成
US12595500B2 (en) 2011-09-26 2026-04-07 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
GB2496375A (en) 2011-10-28 2013-05-15 Kymab Ltd A non-human assay vertebrate comprising human antibody loci and human epitope knock-in, and uses thereof
US9253965B2 (en) 2012-03-28 2016-02-09 Kymab Limited Animal models and therapeutic molecules
GB201122047D0 (en) 2011-12-21 2012-02-01 Kymab Ltd Transgenic animals
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
CN102634534A (zh) * 2012-03-30 2012-08-15 深圳市中联生物科技开发有限公司 基于同源重组的核酸分子克隆方法及相关试剂盒
CA2871505C (en) 2012-04-24 2021-10-12 Gen9, Inc. Methods for sorting nucleic acids and multiplexed preparative in vitro cloning
US20130288320A1 (en) 2012-04-27 2013-10-31 Bioamber Inc. Methods and microorganisms for increasing the biological synthesis of difunctional alkanes
CN102676470B (zh) * 2012-05-29 2014-04-16 吴江近岸蛋白质科技有限公司 一种用于快速基因克隆的酶混合物及制备方法、包含该酶混合物的试剂盒
WO2013191769A1 (en) * 2012-06-22 2013-12-27 Mayo Foundation For Medical Education And Research Genome editing
IL236303B (en) 2012-06-25 2022-07-01 Gen9 Inc Methods for high-throughput nucleic acid assembly and sequencing
MX2015001780A (es) 2012-08-10 2015-09-28 Opx Biotechnologies Inc Microorganismos y métodos para la producción de ácidos grasos y productos derivados de ácidos grasos.
SI2959006T1 (sl) 2013-02-22 2018-10-30 F. Hoffmann-La Roche Ag Uporaba prokariontskega seva, ozdravljenega aminokislinske avksotrofije, za proizvodnjo rekombinantnega polipeptida
US20150119601A1 (en) 2013-03-15 2015-04-30 Opx Biotechnologies, Inc. Monofunctional mcr + 3-hp dehydrogenase
EP3022310B1 (en) 2013-07-19 2019-10-16 Cargill, Incorporated Microorganisms and methods for the production of fatty acids and fatty acid derived products
US11408013B2 (en) 2013-07-19 2022-08-09 Cargill, Incorporated Microorganisms and methods for the production of fatty acids and fatty acid derived products
EP3988649B1 (en) * 2013-09-18 2024-11-27 Kymab Limited Methods, cells and organisms
AU2015324564B2 (en) 2014-06-11 2021-07-01 Duke University Compositions and methods for rapid and dynamic flux control using synthetic metabolic valves
AU2015293825B2 (en) 2014-07-25 2019-06-13 R.P. Scherer Technologies, Llc Improved host cell for producing proteins
EP2993228B1 (en) 2014-09-02 2019-10-09 Cargill, Incorporated Production of fatty acid esters
US10407676B2 (en) 2014-12-09 2019-09-10 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
BR112017021667A2 (pt) 2015-04-09 2018-07-24 Genomatica Inc microorganismos modificados & métodos para produção aperfeiçoada de álcool de crotila
EP3440213A1 (en) * 2016-04-06 2019-02-13 Gene Bridges GmbH Adenoviral vectors
CN106834335B (zh) * 2017-01-11 2020-05-08 浙江科技学院 在酵母菌中实现高准确率基因同源克隆的方法
US11345938B2 (en) 2017-02-02 2022-05-31 Cargill, Incorporated Genetically modified cells that produce C6-C10 fatty acid derivatives
WO2019046350A1 (en) * 2017-08-30 2019-03-07 President And Fellows Of Harvard College ITERATIVE GENOMIC ASSEMBLY
EP3862430A4 (en) * 2018-10-04 2022-06-15 Kaneka Corporation DNA CONSTRUCT FOR USE IN PLANT GENO EDITING
KR102129584B1 (ko) 2018-11-26 2020-07-02 충북대학교 산학협력단 목적 유전자를 선형 벡터에 삽입하는 방법
SG11202111865UA (en) 2019-05-01 2021-11-29 Pact Pharma Inc Compositions and methods for the treatment of cancer using a tet2 engineered t cell therapy
CN110184287B (zh) * 2019-05-24 2024-01-30 华南农业大学 一种制备重组病毒的方法及其应用
CN110218724A (zh) * 2019-06-13 2019-09-10 山西农业大学 猪nr1h3基因过表达载体质粒的构建方法及其表达水平的检测
WO2025199499A1 (en) * 2024-03-22 2025-09-25 Orbit Genomics, Inc. Methods and systems for sample classification

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU4386393A (en) * 1992-05-21 1993-12-13 Genebiomed, Inc. Process for gene targeting and genome manipulations
EP0998574A1 (en) 1997-06-23 2000-05-10 The Rockefeller University Methods of performing homologous recombination based modification of nucleic acids in recombination deficient cells and use of the modified nucleic acids
US6509156B1 (en) 1997-12-05 2003-01-21 Europaisches Laboratorium Fur Molekularoiologie (Embl) DNA cloning method relying on the E. coli recE/recT recombination system
US6355412B1 (en) * 1999-07-09 2002-03-12 The European Molecular Biology Laboratory Methods and compositions for directed cloning and subcloning using homologous recombination

Also Published As

Publication number Publication date
JP2009106295A (ja) 2009-05-21
KR100751587B1 (ko) 2007-08-28
JP4312981B2 (ja) 2009-08-12
CN100469877C (zh) 2009-03-18
ATE305035T1 (de) 2005-10-15
NZ516722A (ja) 2003-12-19
KR20020033726A (ko) 2002-05-07
DK1204740T3 (da) 2006-02-20
ZA200200152B (en) 2003-01-08
WO2001004288A1 (en) 2001-01-18
JP2003504053A (ja) 2003-02-04
DE60022766D1 (de) 2006-02-02
BR0012283A (pt) 2002-06-11
EP1204740A1 (en) 2002-05-15
AU781379B2 (en) 2005-05-19
CN101492694A (zh) 2009-07-29
IL147385A0 (en) 2002-08-14
US6355412B1 (en) 2002-03-12
CA2377938A1 (en) 2001-01-18
HK1046926A1 (en) 2003-01-30
JP5067970B2 (ja) 2012-11-07
HUP0201963A2 (en) 2002-09-28
DE60022766T2 (de) 2006-07-06
YU1502A (sh) 2005-11-28
HU228977B1 (hu) 2013-07-29
MX252928B (es) 2008-01-04
ES2249291T3 (es) 2006-04-01
CN1373803A (zh) 2002-10-09
MXPA02000233A (es) 2003-08-20
HUP0201963A3 (en) 2004-01-28
CA2377938C (en) 2018-01-09
EP1204740B1 (en) 2005-09-21
AU6691100A (en) 2001-01-30
CN101492694B (zh) 2011-08-31

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