PL3930899T3 - Sposób i układ mikroprzepływowy do izolacji cząstek - Google Patents

Sposób i układ mikroprzepływowy do izolacji cząstek

Info

Publication number
PL3930899T3
PL3930899T3 PL20713962.7T PL20713962T PL3930899T3 PL 3930899 T3 PL3930899 T3 PL 3930899T3 PL 20713962 T PL20713962 T PL 20713962T PL 3930899 T3 PL3930899 T3 PL 3930899T3
Authority
PL
Poland
Prior art keywords
particle isolation
microflow system
microflow
isolation
particle
Prior art date
Application number
PL20713962.7T
Other languages
English (en)
Inventor
Gianni Medoro
Original Assignee
Menarini Silicon Biosystems S.P.A.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Menarini Silicon Biosystems S.P.A. filed Critical Menarini Silicon Biosystems S.P.A.
Publication of PL3930899T3 publication Critical patent/PL3930899T3/pl

Links

Classifications

    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40Concentrating samples
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429Signal processing
    • G01N15/1433Signal processing using image recognition
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N15/1468Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle
    • G01N15/147Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle the analysis being performed on a sample stream
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N15/149Optical investigation techniques, e.g. flow cytometry specially adapted for sorting particles, e.g. by their size or optical properties
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02Adapting objects or devices to another
    • B01L2200/026Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B01L2200/027Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06Fluid handling related problems
    • B01L2200/0647Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0652Sorting or classification of particles or molecules
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/06Auxiliary integrated devices, integrated components
    • B01L2300/0627Sensor or part of a sensor is integrated
    • B01L2300/0663Whole sensors
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/08Geometry, shape and general structure
    • B01L2300/0809Geometry, shape and general structure rectangular shaped
    • B01L2300/0816Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/08Geometry, shape and general structure
    • B01L2300/0861Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/08Geometry, shape and general structure
    • B01L2300/0861Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/087Multiple sequential chambers
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/18Means for temperature control
    • B01L2300/1894Cooling means; Cryo cooling
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00Moving or stopping fluids
    • B01L2400/04Moving fluids with specific forces or mechanical means
    • B01L2400/0403Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0424Dielectrophoretic forces
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00Moving or stopping fluids
    • B01L2400/04Moving fluids with specific forces or mechanical means
    • B01L2400/0403Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/043Moving fluids with specific forces or mechanical means specific forces magnetic forces
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00Moving or stopping fluids
    • B01L2400/04Moving fluids with specific forces or mechanical means
    • B01L2400/0403Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0433Moving fluids with specific forces or mechanical means specific forces vibrational forces
    • B01L2400/0436Moving fluids with specific forces or mechanical means specific forces vibrational forces acoustic forces, e.g. surface acoustic waves [SAW]
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00Moving or stopping fluids
    • B01L2400/08Regulating or influencing the flow resistance
    • B01L2400/084Passive control of flow resistance
    • B01L2400/086Passive control of flow resistance using baffles or other fixed flow obstructions
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N2015/1006Investigating individual particles for cytology
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N15/1404Handling flow, e.g. hydrodynamic focusing
    • G01N2015/1415Control of particle position

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Clinical Laboratory Science (AREA)
  • Hematology (AREA)
  • Pathology (AREA)
  • Immunology (AREA)
  • General Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Fluid Mechanics (AREA)
  • Engineering & Computer Science (AREA)
  • Signal Processing (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Electrostatic Separation (AREA)
  • Separation Of Solids By Using Liquids Or Pneumatic Power (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Computer Vision & Pattern Recognition (AREA)
PL20713962.7T 2019-02-26 2020-02-26 Sposób i układ mikroprzepływowy do izolacji cząstek PL3930899T3 (pl)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IT102019000002777A IT201900002777A1 (it) 2019-02-26 2019-02-26 Metodo e sistema microfluidico per l'isolamento di particelle
PCT/IB2020/051647 WO2020174422A1 (en) 2019-02-26 2020-02-26 Microfluidic method and system for the isolation of particles

Publications (1)

Publication Number Publication Date
PL3930899T3 true PL3930899T3 (pl) 2024-06-24

Family

ID=66690772

Family Applications (1)

Application Number Title Priority Date Filing Date
PL20713962.7T PL3930899T3 (pl) 2019-02-26 2020-02-26 Sposób i układ mikroprzepływowy do izolacji cząstek

Country Status (14)

Country Link
US (1) US20220143615A1 (pl)
EP (1) EP3930899B1 (pl)
JP (1) JP7551637B2 (pl)
KR (1) KR102901877B1 (pl)
CN (1) CN113490549B (pl)
AU (1) AU2020229471B2 (pl)
CA (1) CA3130041A1 (pl)
ES (1) ES2984922T3 (pl)
IL (1) IL285834B2 (pl)
IT (1) IT201900002777A1 (pl)
PL (1) PL3930899T3 (pl)
SA (1) SA521430149B1 (pl)
SG (1) SG11202108774XA (pl)
WO (1) WO2020174422A1 (pl)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023283408A1 (en) * 2021-07-09 2023-01-12 Fluent Biosciences Inc. Devices for generating pre-templated instant partitions
CN113996357B (zh) * 2021-10-29 2023-05-23 北京理工大学 微流控芯片管道内部加热条件控制液体定向流动方法
KR102785966B1 (ko) * 2022-03-18 2025-03-26 주식회사 뉴로메카 딥 러닝 알고리즘을 이용한 이미지 기반의 미세유체 세포 분류기 및 미세유체 세포 분류 방법

Family Cites Families (23)

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IT1309430B1 (it) 1999-05-18 2002-01-23 Guerrieri Roberto Metodo ed apparato per la manipolazione di particelle per mezzo delladielettroforesi
ITBO20050481A1 (it) 2005-07-19 2007-01-20 Silicon Biosystems S R L Metodo ed apparato per la manipolazione e/o l'individuazione di particelle
ITBO20050643A1 (it) 2005-10-24 2007-04-25 Si Bio S R L Metodo ed apparato per la manipolazione di particelle in soluzioni conduttive
WO2007102839A2 (en) * 2005-10-27 2007-09-13 Applera Corporation Optoelectronic separation of biomolecules
US20080302732A1 (en) * 2007-05-24 2008-12-11 Hyongsok Soh Integrated fluidics devices with magnetic sorting
JP5834001B2 (ja) 2009-03-17 2015-12-16 シリコン・バイオシステムズ・ソシエタ・ペル・アチオニ 細胞を分離するためのマイクロ流体デバイス
US20110293558A1 (en) * 2010-03-22 2011-12-01 Massachusetts Institute Of Technology Material properties of t cells and related methods and compositions
CN103261436B (zh) * 2010-09-14 2015-03-25 加利福尼亚大学董事会 利用微流体截留涡流从异质溶液中分离细胞的方法
IT1403518B1 (it) 2010-12-22 2013-10-31 Silicon Biosystems Spa Dispositivo microfluidico per la manipolazione di particelle
US9846150B2 (en) * 2011-06-02 2017-12-19 Industry-Academic Cooperation Foundation Yonsei University High efficiency particle separating apparatus and method
US10240186B2 (en) * 2011-07-14 2019-03-26 Fluxion Biosciences, Inc. Devices, systems, and methods for magnetic separation
CN103959037A (zh) * 2011-10-25 2014-07-30 皇家飞利浦有限公司 从血液或其他介质中过滤颗粒
EP2964360B1 (en) * 2013-03-08 2019-09-11 Duke University Devices, systems, and methods for acoustically -enhanced magnetophoresis
US9029109B2 (en) * 2013-08-07 2015-05-12 President And Fellows Of Harvard College Microfluidic vortex-assisted electroporation system and method
EP3058316B1 (en) * 2013-10-18 2019-03-13 The General Hospital Corporation Microfluidic sorting using high gradient magnetic fields
EP3760703A1 (en) * 2013-10-22 2021-01-06 Berkeley Lights, Inc. Microfluidic devices having isolation pens and methods of testing biological micro-objects with same
CN105849561B (zh) * 2013-10-22 2018-09-14 伯克利之光生命科技公司 用于测定生物活性的微流体装置
EP2881739B1 (en) * 2013-12-04 2017-05-17 Menarini Silicon Biosystems S.p.A. Method and kit for determining the genome integrity and/or the quality of a library of dna sequences obtained by deterministic restriction site whole genome amplification
WO2015188171A1 (en) * 2014-06-06 2015-12-10 Berkeley Lights, Inc. Isolating microfluidic structures and trapping bubbles
US10048190B2 (en) * 2015-01-21 2018-08-14 Sbt Instruments Aps Microfluidic particle analysis device
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CN108495712A (zh) * 2015-11-23 2018-09-04 伯克利之光生命科技公司 原位生成的微流体隔离结构、试剂盒及其使用方法
US10717086B2 (en) * 2016-08-29 2020-07-21 The Regents Of The University Of California Integrated system for isolation and emulsification of particles and cells

Also Published As

Publication number Publication date
IL285834B1 (en) 2025-04-01
SA521430149B1 (ar) 2024-08-04
EP3930899A1 (en) 2022-01-05
AU2020229471B2 (en) 2025-03-13
US20220143615A1 (en) 2022-05-12
ES2984922T3 (es) 2024-10-31
IT201900002777A1 (it) 2020-08-26
SG11202108774XA (en) 2021-09-29
WO2020174422A1 (en) 2020-09-03
KR20210145140A (ko) 2021-12-01
JP7551637B2 (ja) 2024-09-17
IL285834B2 (en) 2025-08-01
JP2022521940A (ja) 2022-04-13
CA3130041A1 (en) 2020-09-03
IL285834A (en) 2021-10-31
AU2020229471A1 (en) 2021-09-16
KR102901877B1 (ko) 2025-12-18
CN113490549B (zh) 2023-08-01
CN113490549A (zh) 2021-10-08
EP3930899B1 (en) 2024-02-21

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