PL399993A1 - Preparation of the immunosensor and its use to detect influenza virus - Google Patents
Preparation of the immunosensor and its use to detect influenza virusInfo
- Publication number
- PL399993A1 PL399993A1 PL399993A PL39999312A PL399993A1 PL 399993 A1 PL399993 A1 PL 399993A1 PL 399993 A PL399993 A PL 399993A PL 39999312 A PL39999312 A PL 39999312A PL 399993 A1 PL399993 A1 PL 399993A1
- Authority
- PL
- Poland
- Prior art keywords
- solution
- rinsed
- electrodes
- immunosensor
- influenza virus
- Prior art date
Links
- 241000712461 unidentified influenza virus Species 0.000 title abstract 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 abstract 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 abstract 2
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 abstract 2
- SRZXCOWFGPICGA-UHFFFAOYSA-N 1,6-Hexanedithiol Chemical compound SCCCCCCS SRZXCOWFGPICGA-UHFFFAOYSA-N 0.000 abstract 1
- 101710085938 Matrix protein Proteins 0.000 abstract 1
- 101710127721 Membrane protein Proteins 0.000 abstract 1
- 239000008367 deionised water Substances 0.000 abstract 1
- 229910021641 deionized water Inorganic materials 0.000 abstract 1
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 abstract 1
- 229910000397 disodium phosphate Inorganic materials 0.000 abstract 1
- 235000019800 disodium phosphate Nutrition 0.000 abstract 1
- 239000010931 gold Substances 0.000 abstract 1
- 229910052737 gold Inorganic materials 0.000 abstract 1
- 235000019799 monosodium phosphate Nutrition 0.000 abstract 1
- 239000011780 sodium chloride Substances 0.000 abstract 1
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 abstract 1
- 229910000162 sodium phosphate Inorganic materials 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56983—Viruses
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
- G01N2333/08—RNA viruses
- G01N2333/11—Orthomyxoviridae, e.g. influenza virus
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Peptides Or Proteins (AREA)
Abstract
Przedmiotem wynalazku jest sposób przygotowania immunoczujnika do wykrywania wirusa grypy, gdzie czyste złote elektrody płucze się etanolem i umieszcza się w 10 mM etanolowym roztworze 1,6-heksanoditiolu (HDT) na 20 h w temperaturze pokojowej. Następnie elektrody płucze się kolejno etanolem i wodą dejonizowaną oraz odwraca się dołem do góry i nakrapla 10 µl roztworu złota koloidalnego (GCP) na 18 h w +4°C, w kolejnym etapie elektrody płucze się 0.1 M buforem PBS (145 mM NaCl, 2.7 mM KCl, 8.4 mM NaH2PO4, 1.6 mM Na2HPO4) i nakrapla się 10 µl roztworu przeciwciał anty-M o stężeniu 28 µg/ml w 0.1 M buforze PBS na 20 h w +4°C. W ostatnim etapie na powierzchnie elektrod nakrapla się 10 µl roztworu 0.5% (w/v) BSA w 0.1 M PBS na 2 h w +4°C, tak przygotowane elektrody płucze się buforem PBS i przechowuje w temperaturze +4°C do momentu użycia (nie dłużej niż jeden dzień). Ponadto ujawniono zastosowanie immunoczujnika do wykrywania w roztworze białka M oraz wirusa grypy.The subject of the invention is a method for preparing an immunosensor for detecting influenza virus, where clean gold electrodes are rinsed with ethanol and placed in a 10 mM ethanol solution of 1,6-hexanedithiol (HDT) for 20 h at room temperature. Then, the electrodes are rinsed successively with ethanol and deionized water, turned upside down and 10 µl of colloidal gold solution (GCP) is dropped for 18 h at +4°C. In the next step, the electrodes are rinsed with 0.1 M PBS buffer (145 mM NaCl, 2.7 mM KCl, 8.4 mM NaH2PO4, 1.6 mM Na2HPO4) and 10 µl of anti-M antibody solution with a concentration of 28 µg/ml in 0.1 M PBS buffer is added dropwise for 20 h at +4°C. In the last stage, 10 µl of a solution of 0.5% (w/v) BSA in 0.1 M PBS is dropped onto the electrode surfaces for 2 h at +4°C, the electrodes prepared in this way are rinsed with PBS buffer and stored at +4°C until use ( no longer than one day). Furthermore, the use of an immunosensor for detecting M protein and influenza virus in a solution is disclosed.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PL399993A PL223208B1 (en) | 2012-07-16 | 2012-07-16 | Method for preparing immuno-sensor and its use for the detection of influenza virus |
| PCT/PL2013/000092 WO2014014371A1 (en) | 2012-07-16 | 2013-07-15 | A method of fabrication of an immunosensor and its use for detection of influenza virus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PL399993A PL223208B1 (en) | 2012-07-16 | 2012-07-16 | Method for preparing immuno-sensor and its use for the detection of influenza virus |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| PL399993A1 true PL399993A1 (en) | 2014-01-20 |
| PL223208B1 PL223208B1 (en) | 2016-10-31 |
Family
ID=49034154
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PL399993A PL223208B1 (en) | 2012-07-16 | 2012-07-16 | Method for preparing immuno-sensor and its use for the detection of influenza virus |
Country Status (2)
| Country | Link |
|---|---|
| PL (1) | PL223208B1 (en) |
| WO (1) | WO2014014371A1 (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3356823B1 (en) | 2015-09-28 | 2020-04-22 | Sensdx Sp. z o.o. | A modified bdd substrate and its use as an impedance sensor for detection of biological molecules and a system and method for monitoring pathogens |
| CN106093139A (en) * | 2016-06-29 | 2016-11-09 | 中国科学院长春应用化学研究所 | A kind of detection method of concentration of methanol solution |
| CN108663472B (en) * | 2018-04-20 | 2020-06-19 | 国药集团德众（佛山）药业有限公司 | Identification method of rhododendron anthopogonoides essential oil |
| PL431069A1 (en) * | 2019-09-09 | 2021-03-22 | Sensdx Spółka Akcyjna | Influenza virus detection kit containing a buffer, a linker and an amino acid sequence and detection method |
| CN114935648A (en) * | 2022-04-19 | 2022-08-23 | 谱瑞前海(深圳)智能科技有限公司 | Method for generating electric signal from epidemic virus immunodetection result |
-
2012
- 2012-07-16 PL PL399993A patent/PL223208B1/en unknown
-
2013
- 2013-07-15 WO PCT/PL2013/000092 patent/WO2014014371A1/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| WO2014014371A1 (en) | 2014-01-23 |
| PL223208B1 (en) | 2016-10-31 |
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