PL400501A1 - Sposób izolowania DNA drobnoustrojów z krwi - Google Patents

Sposób izolowania DNA drobnoustrojów z krwi

Info

Publication number
PL400501A1
PL400501A1 PL400501A PL40050112A PL400501A1 PL 400501 A1 PL400501 A1 PL 400501A1 PL 400501 A PL400501 A PL 400501A PL 40050112 A PL40050112 A PL 40050112A PL 400501 A1 PL400501 A1 PL 400501A1
Authority
PL
Poland
Prior art keywords
blood
microorganisms
isolating dna
lysis
dna
Prior art date
Application number
PL400501A
Other languages
English (en)
Other versions
PL219490B1 (pl
Inventor
Tomasz Gosiewski
Monika Brzychczy-Włoch
Original Assignee
Uniwersytet Jagiellonski
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Uniwersytet Jagiellonski filed Critical Uniwersytet Jagiellonski
Priority to PL400501A priority Critical patent/PL219490B1/pl
Priority to US14/422,860 priority patent/US9879311B2/en
Priority to PCT/PL2013/000109 priority patent/WO2014031018A1/en
Priority to EP13774254.0A priority patent/EP2888364B1/en
Priority to ES13774254.0T priority patent/ES2652267T3/es
Publication of PL400501A1 publication Critical patent/PL400501A1/pl
Publication of PL219490B1 publication Critical patent/PL219490B1/pl

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Sposób umożliwia jednoczesne izolowanie DNA bakteryjnego i grzybiczego z krwi. W sposobie wykorzystuje się lizę enzymatyczną, lizy mechaniczną oraz lizę termiczną.
PL400501A 2012-08-24 2012-08-24 Sposób izolowania DNA drobnoustrojów z krwi PL219490B1 (pl)

Priority Applications (5)

Application Number Priority Date Filing Date Title
PL400501A PL219490B1 (pl) 2012-08-24 2012-08-24 Sposób izolowania DNA drobnoustrojów z krwi
US14/422,860 US9879311B2 (en) 2012-08-24 2013-08-23 Method for efficient isolation of microbial DNA from blood
PCT/PL2013/000109 WO2014031018A1 (en) 2012-08-24 2013-08-23 Method for efficient isolation of microbial dna from blood
EP13774254.0A EP2888364B1 (en) 2012-08-24 2013-08-23 Method for efficient isolation of microbial dna from blood
ES13774254.0T ES2652267T3 (es) 2012-08-24 2013-08-23 Método para el aislamiento eficiente del ADN microbiano de la sangre

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL400501A PL219490B1 (pl) 2012-08-24 2012-08-24 Sposób izolowania DNA drobnoustrojów z krwi

Publications (2)

Publication Number Publication Date
PL400501A1 true PL400501A1 (pl) 2014-03-03
PL219490B1 PL219490B1 (pl) 2015-05-29

Family

ID=49322673

Family Applications (1)

Application Number Title Priority Date Filing Date
PL400501A PL219490B1 (pl) 2012-08-24 2012-08-24 Sposób izolowania DNA drobnoustrojów z krwi

Country Status (5)

Country Link
US (1) US9879311B2 (pl)
EP (1) EP2888364B1 (pl)
ES (1) ES2652267T3 (pl)
PL (1) PL219490B1 (pl)
WO (1) WO2014031018A1 (pl)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR3035409B1 (fr) * 2015-04-21 2020-01-24 Fondation Mediterranee Infection Methode d'extraction d'adn comprenant un traitement de degradation enzymatique d'un echantillon biologique
US11959125B2 (en) 2016-09-15 2024-04-16 Sun Genomics, Inc. Universal method for extracting nucleic acid molecules from a diverse population of one or more types of microbes in a sample
WO2018053308A1 (en) * 2016-09-15 2018-03-22 Sun Genomics Llc Universal method for extracting nucleic acid molecules from a diverse population of one or more types of microbes in a sample
US20200370036A1 (en) * 2017-08-04 2020-11-26 Microbedx, Inc. Methods for Lysis of Cells Within a Sample
CN113637668A (zh) * 2021-10-15 2021-11-12 微岩医学科技(北京)有限公司 一种同时提取血浆和血细胞病原菌dna的试剂盒及应用
CN119410628B (zh) * 2024-12-09 2025-12-30 武汉大学中南医院 一种增强型红细胞裂解液、红细胞dna提取试剂盒及方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6605439B2 (en) 1995-08-17 2003-08-12 Eberhard-Karls Universität Tübingen Universitätsklinikum Extraction, amplification and sequential hybridization of fungal cell DNA and process for detection of fungal cells in clinical material
WO1997007238A2 (de) * 1995-08-17 1997-02-27 Eberhard-Karls-Universität Tübingen Extraktion, amplifikation und sequentielle hybridisierung von pilzzellen-dna sowie verfahren zum nachweisen von pilzzellen in klinischem material
EP2290099B1 (en) 1998-02-02 2012-10-10 QIAGEN North American Holdings, Inc. Processes for isolating, amplifying and characterizing DNA
US20020068280A1 (en) 2000-12-06 2002-06-06 Jeff Fairman Compositions and methods for DNA purification from whole blood
US8481265B2 (en) 2007-08-02 2013-07-09 Universite Laval Concentration and enrichment of microbial cells and microbial nucleic acids from bodily fluids
WO2009042457A1 (en) 2007-09-21 2009-04-02 Streck, Inc. Nucleic acid isolation in preserved whole blood
WO2012050787A1 (en) 2010-09-29 2012-04-19 Ibis Biosciences, Inc. Fungal nucleic acid extraction

Also Published As

Publication number Publication date
EP2888364B1 (en) 2017-11-15
US20150240290A1 (en) 2015-08-27
ES2652267T3 (es) 2018-02-01
PL219490B1 (pl) 2015-05-29
US9879311B2 (en) 2018-01-30
EP2888364A1 (en) 2015-07-01
WO2014031018A1 (en) 2014-02-27

Similar Documents

Publication Publication Date Title
AU2011372711B2 (en) Method for processing mesenchymal stem cells and the use thereof in the treatment of diseases associated with oxidative stress
AU2019232913A1 (en) Methods and processes for non-invasive assessment of genetic variations
MX375786B (es) Biosintesis microbiana de ergotioneina.
MX2014003979A (es) Nucleosidos, nucleotidos, y acidos nucleicos modificados, y usos de los mismos.
CA2877331C (en) Methods and processes for non-invasive assessment of genetic variations
MX2016000997A (es) Metodos y composiciones para detectar contaminacion bacteriana.
BR112016016916A2 (pt) método de tratamento de angiodema hereditário (hae)
UY34342A (es) ?derivados de pirrolopirimidina y purina?.
WO2015071474A3 (en) Crispr-cas system materials and methods
BRPI1011934A8 (pt) Composição
WO2013052913A3 (en) Methods and processes for non-invasive assessment of genetic variations
UY34227A (es) Acido nucleico recombinante que codifica el gen de la toxina axmi270, vectores, células, plantas y sus métodos de empleo
CL2015003479A1 (es) Métodos y composiciones de fermentación bacterial.
EP3263697A4 (en) Culture medium for culturing mesenchymal stem cell, method for culturing mesenchymal stem cell, and mesenchymal stem cell
TW201612308A (en) Collector architecture layout design
EP2880050A4 (en) NOVEL ENZYMES, ENZYME COMPONENTS AND USES THEREOF
AU2013225816A8 (en) Compositions and methods for treating Type III Gaucher Disease
PH12020550654A1 (en) Asymmetric pcr methods
EP3071705A4 (en) Detection, isolation and identification of microorganisms
MX349801B (es) Sustratos de enzimas de ácido nucleico.
WO2012024304A3 (en) Bioreactor system
WO2013163297A8 (en) Modified glycoproteins
PL2971017T3 (pl) Sposób traktowania mikroorganizmów podczas propagacji, kondycjonowania i fermentacji z użyciem ekstraktów kwasu chmielowego i kwasu organicznego
EP3272853A4 (en) Culturing method for microorganism, and culture device
EP3149187A4 (en) Modified microorganisms comprising an optimized system for oligosaccharide utilization and methods of using same