PL400501A1 - Sposób izolowania DNA drobnoustrojów z krwi - Google Patents

Sposób izolowania DNA drobnoustrojów z krwi

Info

Publication number
PL400501A1
PL400501A1 PL400501A PL40050112A PL400501A1 PL 400501 A1 PL400501 A1 PL 400501A1 PL 400501 A PL400501 A PL 400501A PL 40050112 A PL40050112 A PL 40050112A PL 400501 A1 PL400501 A1 PL 400501A1
Authority
PL
Poland
Prior art keywords
blood
microorganisms
isolating dna
lysis
dna
Prior art date
Application number
PL400501A
Other languages
English (en)
Other versions
PL219490B1 (pl
Inventor
Tomasz Gosiewski
Monika Brzychczy-Włoch
Original Assignee
Uniwersytet Jagiellonski
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Uniwersytet Jagiellonski filed Critical Uniwersytet Jagiellonski
Priority to PL400501A priority Critical patent/PL219490B1/pl
Priority to ES13774254.0T priority patent/ES2652267T3/es
Priority to EP13774254.0A priority patent/EP2888364B1/en
Priority to US14/422,860 priority patent/US9879311B2/en
Priority to PCT/PL2013/000109 priority patent/WO2014031018A1/en
Publication of PL400501A1 publication Critical patent/PL400501A1/pl
Publication of PL219490B1 publication Critical patent/PL219490B1/pl

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Sposób umożliwia jednoczesne izolowanie DNA bakteryjnego i grzybiczego z krwi. W sposobie wykorzystuje się lizę enzymatyczną, lizy mechaniczną oraz lizę termiczną.
PL400501A 2012-08-24 2012-08-24 Sposób izolowania DNA drobnoustrojów z krwi PL219490B1 (pl)

Priority Applications (5)

Application Number Priority Date Filing Date Title
PL400501A PL219490B1 (pl) 2012-08-24 2012-08-24 Sposób izolowania DNA drobnoustrojów z krwi
ES13774254.0T ES2652267T3 (es) 2012-08-24 2013-08-23 Método para el aislamiento eficiente del ADN microbiano de la sangre
EP13774254.0A EP2888364B1 (en) 2012-08-24 2013-08-23 Method for efficient isolation of microbial dna from blood
US14/422,860 US9879311B2 (en) 2012-08-24 2013-08-23 Method for efficient isolation of microbial DNA from blood
PCT/PL2013/000109 WO2014031018A1 (en) 2012-08-24 2013-08-23 Method for efficient isolation of microbial dna from blood

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL400501A PL219490B1 (pl) 2012-08-24 2012-08-24 Sposób izolowania DNA drobnoustrojów z krwi

Publications (2)

Publication Number Publication Date
PL400501A1 true PL400501A1 (pl) 2014-03-03
PL219490B1 PL219490B1 (pl) 2015-05-29

Family

ID=49322673

Family Applications (1)

Application Number Title Priority Date Filing Date
PL400501A PL219490B1 (pl) 2012-08-24 2012-08-24 Sposób izolowania DNA drobnoustrojów z krwi

Country Status (5)

Country Link
US (1) US9879311B2 (pl)
EP (1) EP2888364B1 (pl)
ES (1) ES2652267T3 (pl)
PL (1) PL219490B1 (pl)
WO (1) WO2014031018A1 (pl)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR3035409B1 (fr) * 2015-04-21 2020-01-24 Fondation Mediterranee Infection Methode d'extraction d'adn comprenant un traitement de degradation enzymatique d'un echantillon biologique
US11959125B2 (en) 2016-09-15 2024-04-16 Sun Genomics, Inc. Universal method for extracting nucleic acid molecules from a diverse population of one or more types of microbes in a sample
US10428370B2 (en) * 2016-09-15 2019-10-01 Sun Genomics, Inc. Universal method for extracting nucleic acid molecules from a diverse population of one or more types of microbes in a sample
IL272403B2 (en) * 2017-08-04 2025-08-01 Microbedx Inc Methods for lysis of cells within a sample
CN113637668A (zh) * 2021-10-15 2021-11-12 微岩医学科技(北京)有限公司 一种同时提取血浆和血细胞病原菌dna的试剂盒及应用
CN119410628B (zh) * 2024-12-09 2025-12-30 武汉大学中南医院 一种增强型红细胞裂解液、红细胞dna提取试剂盒及方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6605439B2 (en) 1995-08-17 2003-08-12 Eberhard-Karls Universität Tübingen Universitätsklinikum Extraction, amplification and sequential hybridization of fungal cell DNA and process for detection of fungal cells in clinical material
EP0894870B1 (de) * 1995-08-17 2012-10-10 Myconostica Limited Extraktion von Pilzzellen-DNA aus klinischem Material
DE69938774D1 (de) 1998-02-02 2008-07-03 Qiagen North American Holdings Verfahren zur isolierung, amplifizierung und charakterisierung von dns
US20020068280A1 (en) 2000-12-06 2002-06-06 Jeff Fairman Compositions and methods for DNA purification from whole blood
JP2010535016A (ja) 2007-08-02 2010-11-18 ユニヴェルシテ・ラヴァル 体液から微生物細胞および微生物核酸の濃縮および富化
WO2009042457A1 (en) 2007-09-21 2009-04-02 Streck, Inc. Nucleic acid isolation in preserved whole blood
WO2012050787A1 (en) 2010-09-29 2012-04-19 Ibis Biosciences, Inc. Fungal nucleic acid extraction

Also Published As

Publication number Publication date
ES2652267T3 (es) 2018-02-01
EP2888364A1 (en) 2015-07-01
US20150240290A1 (en) 2015-08-27
EP2888364B1 (en) 2017-11-15
PL219490B1 (pl) 2015-05-29
US9879311B2 (en) 2018-01-30
WO2014031018A1 (en) 2014-02-27

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