PL400598A1 - Sposób identyfikacji bakterii Gram-ujemnych z zastosowaniem metody MALDI-TOF MS - Google Patents

Sposób identyfikacji bakterii Gram-ujemnych z zastosowaniem metody MALDI-TOF MS

Info

Publication number
PL400598A1
PL400598A1 PL400598A PL40059812A PL400598A1 PL 400598 A1 PL400598 A1 PL 400598A1 PL 400598 A PL400598 A PL 400598A PL 40059812 A PL40059812 A PL 40059812A PL 400598 A1 PL400598 A1 PL 400598A1
Authority
PL
Poland
Prior art keywords
maldi
gram
tof
negative bacteria
identification
Prior art date
Application number
PL400598A
Other languages
English (en)
Other versions
PL223203B1 (pl
Inventor
Sabina Koj
Tomasz Niedziela
Czesław Ługowski
Original Assignee
Wroclawskie Centrum Badan Eit + Spólka Z Ograniczona Odpowiedzialnoscia
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wroclawskie Centrum Badan Eit + Spólka Z Ograniczona Odpowiedzialnoscia filed Critical Wroclawskie Centrum Badan Eit + Spólka Z Ograniczona Odpowiedzialnoscia
Priority to PL400598A priority Critical patent/PL223203B1/pl
Priority to PCT/PL2013/000112 priority patent/WO2014035270A1/en
Publication of PL400598A1 publication Critical patent/PL400598A1/pl
Publication of PL223203B1 publication Critical patent/PL223203B1/pl

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Toxicology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Przedmiotem wynalazku jest sposób identyfikacji chemotypów lipopolisacharydowych bakterii Gram-ujemnych w oparciu o profil endotoksynowy pelnych komórek bakteryjnych uzyskany z zastosowaniem metody MALDI-TOF MS. Profil pochodzacy od lipopolisacharydów (LPS) dla preparatów mas bakteryjnych uzyskuje sie w trybie jonizacji ujemnej spektrometru MALDI-TOF z zastosowaniem DHB jako matrycy. Przedmiotowe rozwiazanie moze sluzyc do analizy próbek klinicznych, diagnozy infekcji powodowanych przez bakterie Gram-ujemne oraz miec zastosowanie jako narzedzie w terapii nakierowanej na antygen O z pomoca swoistych przeciwcial.
PL400598A 2012-08-31 2012-08-31 Sposób identyfikacji chemotypów lipopolisacharydowych bakterii Gram-ujemnych z zastosowaniem metody MALDI-TOF MS PL223203B1 (pl)

Priority Applications (2)

Application Number Priority Date Filing Date Title
PL400598A PL223203B1 (pl) 2012-08-31 2012-08-31 Sposób identyfikacji chemotypów lipopolisacharydowych bakterii Gram-ujemnych z zastosowaniem metody MALDI-TOF MS
PCT/PL2013/000112 WO2014035270A1 (en) 2012-08-31 2013-08-30 A method of identifying gram-negative bacteria using the maldi-tof ms method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL400598A PL223203B1 (pl) 2012-08-31 2012-08-31 Sposób identyfikacji chemotypów lipopolisacharydowych bakterii Gram-ujemnych z zastosowaniem metody MALDI-TOF MS

Publications (2)

Publication Number Publication Date
PL400598A1 true PL400598A1 (pl) 2014-03-03
PL223203B1 PL223203B1 (pl) 2016-10-31

Family

ID=49510477

Family Applications (1)

Application Number Title Priority Date Filing Date
PL400598A PL223203B1 (pl) 2012-08-31 2012-08-31 Sposób identyfikacji chemotypów lipopolisacharydowych bakterii Gram-ujemnych z zastosowaniem metody MALDI-TOF MS

Country Status (2)

Country Link
PL (1) PL223203B1 (pl)
WO (1) WO2014035270A1 (pl)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9273339B2 (en) 2011-01-03 2016-03-01 University Of Maryland, Baltimore Methods for identifying bacteria
PL404247A1 (pl) 2013-06-07 2014-12-08 Wrocławskie Centrum Badań Eit + Spółka Z Ograniczoną Odpowiedzialnością Koniugat oligocukru LOS Bordetella pertussis i toksyny krztuśca i jego zastosowanie w profilaktyce i leczeniu zakażeń wywoływanych przez Bordetella pertussis
GB2549819B (en) * 2014-05-30 2019-04-17 Csir Method and device for detection of E. coli
GB201703261D0 (en) 2017-02-28 2017-04-12 Imp Innovations Ltd Method of detection

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5059527A (en) * 1989-12-08 1991-10-22 White David C Detection of endotoxins of gram negative bacteria
US6177266B1 (en) 1996-04-29 2001-01-23 The United States Of America As Represented By The Secretary Of The Army Rapid identification of bacteria by mass spectrometry
US7684934B2 (en) 2003-06-06 2010-03-23 The United States Of America As Represented By The Department Of Health And Human Services Pattern recognition of whole cell mass spectra
EP1942194A1 (en) 2007-01-08 2008-07-09 Université René Descartes Method for identifying a germ isolated from a clinical sample
US9273339B2 (en) * 2011-01-03 2016-03-01 University Of Maryland, Baltimore Methods for identifying bacteria

Also Published As

Publication number Publication date
WO2014035270A1 (en) 2014-03-06
WO2014035270A8 (en) 2015-02-12
PL223203B1 (pl) 2016-10-31

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