PL401707A1 - Metoda identyfikacji wirusów RNA oraz jej zastosowanie - Google Patents

Metoda identyfikacji wirusów RNA oraz jej zastosowanie

Info

Publication number
PL401707A1
PL401707A1 PL401707A PL40170712A PL401707A1 PL 401707 A1 PL401707 A1 PL 401707A1 PL 401707 A PL401707 A PL 401707A PL 40170712 A PL40170712 A PL 40170712A PL 401707 A1 PL401707 A1 PL 401707A1
Authority
PL
Poland
Prior art keywords
rna viruses
viruses
identification
relates
application
Prior art date
Application number
PL401707A
Other languages
English (en)
Other versions
PL225633B1 (pl
Inventor
Krzysztof Pyrć
Karol Stożek
Jan Potempa
Original Assignee
Uniwersytet Jagielloński
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Uniwersytet Jagielloński filed Critical Uniwersytet Jagielloński
Priority to PL401707A priority Critical patent/PL225633B1/pl
Priority to PCT/PL2013/050032 priority patent/WO2014081323A2/en
Publication of PL401707A1 publication Critical patent/PL401707A1/pl
Publication of PL225633B1 publication Critical patent/PL225633B1/pl

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Wynalazek dotyczy metody identyfikacji wirusów RNA polegającej na wykorzystaniu krótkich 4-10 nukleotydowych elementów DNA, jako miejsc przyłączenia specyficznych starterów w reakcji PCR w reakcji odwrotnej transkrypcji oraz syntezy drugiej nici, co umożliwia dodanie do fragmentu kwasu nukleinowego syntetycznych adapterów pozwalających na późniejszą amplifikację materiału genetycznego wirusów RNA. Wynalazek także dotyczy zastosowania metody identyfikacji wirusów RNA, w tym znanych patogenów i nowych patogenów, które w związku z naturalną zmiennością nie mogą zostać wykryte metodami standardowymi.
PL401707A 2012-11-22 2012-11-22 Metoda identyfikacji wirusów RNA PL225633B1 (pl)

Priority Applications (2)

Application Number Priority Date Filing Date Title
PL401707A PL225633B1 (pl) 2012-11-22 2012-11-22 Metoda identyfikacji wirusów RNA
PCT/PL2013/050032 WO2014081323A2 (en) 2012-11-22 2013-11-22 Method of rna viruses identification and its application

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL401707A PL225633B1 (pl) 2012-11-22 2012-11-22 Metoda identyfikacji wirusów RNA

Publications (2)

Publication Number Publication Date
PL401707A1 true PL401707A1 (pl) 2014-05-26
PL225633B1 PL225633B1 (pl) 2017-05-31

Family

ID=50001231

Family Applications (1)

Application Number Title Priority Date Filing Date
PL401707A PL225633B1 (pl) 2012-11-22 2012-11-22 Metoda identyfikacji wirusów RNA

Country Status (2)

Country Link
PL (1) PL225633B1 (pl)
WO (1) WO2014081323A2 (pl)

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US5104792A (en) * 1989-12-21 1992-04-14 The United States Of America As Represented By The Department Of Health And Human Services Method for amplifying unknown nucleic acid sequences
EP1007730A4 (en) 1996-08-30 2004-04-28 Invitrogen Corp METHODS OF IDENTIFYING AND ISOLATING SPECIFIC NUCLEOTIDE SEQUENCES IN cDNA AND GENOMIC DNA
GB2319082A (en) 1996-09-27 1998-05-13 Consiglio Nazionale Ricerche Differential screening of gene expression by random primed reverse transcription - polymerase chain reaction (RP RT-PCR)
US6706476B1 (en) * 2000-08-22 2004-03-16 Azign Bioscience A/S Process for amplifying and labeling single stranded cDNA by 5′ ligated adaptor mediated amplification
WO2002103007A1 (en) 2001-06-19 2002-12-27 Eisai C0. Ltd. Method of uniformizing dna fragment contents and subtraction method
WO2003040412A1 (en) 2001-11-05 2003-05-15 California Institute Of Technology A non-metric tool for predicting gene relationships from expression data
JP2005515792A (ja) 2002-01-29 2005-06-02 グローバル ジェノミクス アクティエボラーグ 核酸を操作するための方法および手段
JP4311003B2 (ja) 2002-12-02 2009-08-12 アイシン精機株式会社 原核生物の遺伝子発現解析方法
EP1618214A4 (en) * 2003-04-25 2008-02-13 Becton Dickinson Co DETECTION OF TYPES 1 AND 2 OF HERPES SIMPLEX VIRUS BY AMPLIFICATION OF NUCLEIC ACID

Also Published As

Publication number Publication date
WO2014081323A2 (en) 2014-05-30
PL225633B1 (pl) 2017-05-31
WO2014081323A3 (en) 2014-08-07

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