PL403996A1 - Sposób jednoczesnej detekcji bakterii i grzybów w preparacie biologicznym metodą PCR, startery oraz zestaw do detekcji bakterii i grzybów - Google Patents

Sposób jednoczesnej detekcji bakterii i grzybów w preparacie biologicznym metodą PCR, startery oraz zestaw do detekcji bakterii i grzybów

Info

Publication number
PL403996A1
PL403996A1 PL403996A PL40399613A PL403996A1 PL 403996 A1 PL403996 A1 PL 403996A1 PL 403996 A PL403996 A PL 403996A PL 40399613 A PL40399613 A PL 40399613A PL 403996 A1 PL403996 A1 PL 403996A1
Authority
PL
Poland
Prior art keywords
fungi
bacteria
detection
simultaneous detection
pcr primers
Prior art date
Application number
PL403996A
Other languages
English (en)
Other versions
PL228161B1 (pl
Inventor
Tomasz Gosiewski
Monika Brzychczy-Włoch
Agata Pietrzyk
Małgorzata Bulanda
Original Assignee
Uniwersytet Jagielloński
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Uniwersytet Jagielloński filed Critical Uniwersytet Jagielloński
Priority to PL403996A priority Critical patent/PL228161B1/pl
Priority to PCT/PL2014/050029 priority patent/WO2014189398A1/en
Priority to PL14736045T priority patent/PL2999798T3/pl
Priority to US14/892,458 priority patent/US10415096B2/en
Priority to ES14736045.7T priority patent/ES2618834T3/es
Priority to EP14736045.7A priority patent/EP2999798B1/en
Publication of PL403996A1 publication Critical patent/PL403996A1/pl
Publication of PL228161B1 publication Critical patent/PL228161B1/pl

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Mycology (AREA)
  • Botany (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Przedmiotem zgłoszenia jest sposób równoczesnej detekcji bakterii i grzybów w próbce materiału biologicznego, w którym DNA zawarte w próbce materiału biologicznego poddaje się amplifikacji w reakcji PCR w czasie rzeczywistym w systemie multipleksowym, z zastosowaniem w pierwszym etapie starterów specyficznych dla bakterii i starterów specyficznych dla grzybów, a w drugim etapie powstały DNA jest amplifikowany z zastosowaniem starterów i sond różnicujących grzyby na grupę grzybów pleśniowych i drożdżopodobnych i bakterie na Gram dodatnie i Gram ujemne. Wynalazek obejmuje także nowe oligonukleotydowe startery do detekcji bakterii i grzybów metodą PCR oraz zestaw do równoczesnej detekcji bakterii i grzybów.
PL403996A 2013-05-21 2013-05-21 Sposób jednoczesnej detekcji bakterii i grzybów w preparacie biologicznym metoda PCR oraz zestaw do detekcji bakterii i grzybów w preparacie biologicznym metoda PCR PL228161B1 (pl)

Priority Applications (6)

Application Number Priority Date Filing Date Title
PL403996A PL228161B1 (pl) 2013-05-21 2013-05-21 Sposób jednoczesnej detekcji bakterii i grzybów w preparacie biologicznym metoda PCR oraz zestaw do detekcji bakterii i grzybów w preparacie biologicznym metoda PCR
PCT/PL2014/050029 WO2014189398A1 (en) 2013-05-21 2014-05-21 Method for simultaneous detection of bacteria and fungi in a biological preparation by pcr, primers as well as bacteria and fungi detection kit
PL14736045T PL2999798T3 (pl) 2013-05-21 2014-05-21 Sposób jednoczesnej detekcji bakterii i grzybów w preparacie biologicznym metodą PCR, startery oraz zestaw do detekcji bakterii i grzybów
US14/892,458 US10415096B2 (en) 2013-05-21 2014-05-21 Method for simultaneous detection of bacteria and fungi in a biological preparation by PCR, primers as well as bacteria and fungi detection kit
ES14736045.7T ES2618834T3 (es) 2013-05-21 2014-05-21 Método para la detección simultánea de bacterias y hongos en una preparación biológica mediante PCR, cebadores así como kit de detección de bacterias y hongos
EP14736045.7A EP2999798B1 (en) 2013-05-21 2014-05-21 Method for simultaneous detection of bacteria and fungi in a biological preparation by pcr, primers as well as bacteria and fungi detection kit

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL403996A PL228161B1 (pl) 2013-05-21 2013-05-21 Sposób jednoczesnej detekcji bakterii i grzybów w preparacie biologicznym metoda PCR oraz zestaw do detekcji bakterii i grzybów w preparacie biologicznym metoda PCR

Publications (2)

Publication Number Publication Date
PL403996A1 true PL403996A1 (pl) 2014-11-24
PL228161B1 PL228161B1 (pl) 2018-02-28

Family

ID=51134194

Family Applications (2)

Application Number Title Priority Date Filing Date
PL403996A PL228161B1 (pl) 2013-05-21 2013-05-21 Sposób jednoczesnej detekcji bakterii i grzybów w preparacie biologicznym metoda PCR oraz zestaw do detekcji bakterii i grzybów w preparacie biologicznym metoda PCR
PL14736045T PL2999798T3 (pl) 2013-05-21 2014-05-21 Sposób jednoczesnej detekcji bakterii i grzybów w preparacie biologicznym metodą PCR, startery oraz zestaw do detekcji bakterii i grzybów

Family Applications After (1)

Application Number Title Priority Date Filing Date
PL14736045T PL2999798T3 (pl) 2013-05-21 2014-05-21 Sposób jednoczesnej detekcji bakterii i grzybów w preparacie biologicznym metodą PCR, startery oraz zestaw do detekcji bakterii i grzybów

Country Status (5)

Country Link
US (1) US10415096B2 (pl)
EP (1) EP2999798B1 (pl)
ES (1) ES2618834T3 (pl)
PL (2) PL228161B1 (pl)
WO (1) WO2014189398A1 (pl)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017009693A1 (en) 2015-07-10 2017-01-19 Uniwersytet Jagielloński 16s ribosomal rna universal primers and use thereof in microbiological analysis and diagnostics

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES3030837T3 (en) * 2017-08-24 2025-07-02 Roche Diagnostics Gmbh Electrochemical detection of bacterial and/or fungal infections
US20190062809A1 (en) 2017-08-24 2019-02-28 Clinical Micro Sensors, Inc. (dba GenMark Diagnostics, Inc.) Electrochemical detection of bacterial and/or fungal infections
US20220195496A1 (en) * 2020-12-17 2022-06-23 Karius, Inc. Sequencing microbial cell-free dna from asymptomatic individuals

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US750000A (en) 1904-01-19 Eugene p
US7718354B2 (en) * 2001-03-02 2010-05-18 Ibis Biosciences, Inc. Methods for rapid identification of pathogens in humans and animals
GB0601302D0 (en) * 2006-01-23 2006-03-01 Semikhodskii Andrei Diagnostic methods and apparatus
GB0621864D0 (en) * 2006-11-02 2006-12-13 Univ Manchester Assay for fungal infection
FR2909099B1 (fr) 2006-11-24 2012-10-19 Univ Aix Marseille Ii Methode de diagnostic et de suivi d'une vaginose bacterienne par quantification moleculaire.
EP1978111B1 (en) 2007-04-02 2013-03-27 Gen-Probe Incorporated Compositions, kits and related methods for the detection and/or monitoring of Pseudomonas aeruginosa
CZ301112B6 (cs) 2007-04-23 2009-11-11 Výzkumný ústav veterinárního lékarství, v.v.i. Zpusob detekce a kvantifikace Mycobacterium avium subspecies paratuberculosis polymerázovou retezovou reakcí v reálném case
EP2388322B1 (en) * 2009-01-15 2017-10-25 Hokkaido Mitsui Chemicals, Inc. Enzymatic preparation containing thermostable dna polymerase, process for producing same, and method for detecting analyte organism
AU2011227110B2 (en) 2010-03-19 2016-01-28 The Translational Genomics Research Institute Methods, kits and compositions for detection of MRSA

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017009693A1 (en) 2015-07-10 2017-01-19 Uniwersytet Jagielloński 16s ribosomal rna universal primers and use thereof in microbiological analysis and diagnostics

Also Published As

Publication number Publication date
PL2999798T3 (pl) 2017-07-31
US20160177378A1 (en) 2016-06-23
EP2999798A1 (en) 2016-03-30
US10415096B2 (en) 2019-09-17
EP2999798B1 (en) 2016-12-28
WO2014189398A1 (en) 2014-11-27
PL228161B1 (pl) 2018-02-28
ES2618834T3 (es) 2017-06-22

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