PL404497A1 - The method of determining the relationship of Staphylococcus strains - Google Patents

The method of determining the relationship of Staphylococcus strains

Info

Publication number
PL404497A1
PL404497A1 PL404497A PL40449713A PL404497A1 PL 404497 A1 PL404497 A1 PL 404497A1 PL 404497 A PL404497 A PL 404497A PL 40449713 A PL40449713 A PL 40449713A PL 404497 A1 PL404497 A1 PL 404497A1
Authority
PL
Poland
Prior art keywords
staphylococcus
determining
degree
hyptf
gene
Prior art date
Application number
PL404497A
Other languages
Polish (pl)
Other versions
PL226609B1 (en
Inventor
Michał Bukowski
Klaudia Polakowska
Weronika Helbin
Agnieszka Sitarska
Kinga Nytko
Benedykt Władyka
Original Assignee
Uniwersytet Jagielloński
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Uniwersytet Jagielloński filed Critical Uniwersytet Jagielloński
Priority to PL404497A priority Critical patent/PL226609B1/en
Priority to PCT/PL2014/050038 priority patent/WO2014209145A1/en
Publication of PL404497A1 publication Critical patent/PL404497A1/en
Publication of PL226609B1 publication Critical patent/PL226609B1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Sposób określania stopnia pokrewieństwa szczepów z rodzaju Staphylococcus, charakteryzuje się tym, że izoluje się sekwencję genu hyptf lub jego fragmentu z genomu typowanego szczepu bakteryjnego, porównuje się wyizolowan sekwencję z odpowiadającą jej sekwencją należącą do genu hyptf ze znanego szczepu bakterii rodzaju Staphylococcus i określa stopień podobieństwa tych sekwencji, przy czym ustalony stopień podobieństwa sekwencji genu hyptf jest miarą pokrewieństwa pomiędzy typowanym szczepem bakteryjnym ze znanym szczepem bakterii z rodzaju Staphylococcus. Przedmiotem zgłoszenia jest też zastosowanie polinukleotydy zawierającego gen hyptf lub jego fragment do określenia stopnia szczepów z rodzaju Staphylococcus oraz zestaw starterów do określania stopnia pokrewieństwa szczepów z rodzaju Staphylococcus techniką RFLP.The method of determining the degree of kinship of the Staphylococcus strain is characterized by the isolation of the hyptf gene sequence or a fragment thereof from the genome of the typed bacterial strain, comparing the isolated sequence with the corresponding sequence belonging to the hyptf gene from the known Staphylococcus strain and determining the degree of similarity of these sequences, wherein the determined degree of sequence similarity of the hyptf gene is a measure of the relationship between the typed bacterial strain and a known bacterial strain of the genus Staphylococcus. The subject of the application is also the use of a polynucleotide containing the hyptf gene or a fragment thereof to determine the degree of strains of the Staphylococcus genus and a set of primers for determining the degree of relatedness of the Staphylococcus genus by RFLP.

PL404497A 2013-06-28 2013-06-28 The use of polynucleotide, a set of primers and the method of determining the degree of relationship of Staphylococcus strains PL226609B1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
PL404497A PL226609B1 (en) 2013-06-28 2013-06-28 The use of polynucleotide, a set of primers and the method of determining the degree of relationship of Staphylococcus strains
PCT/PL2014/050038 WO2014209145A1 (en) 2013-06-28 2014-06-25 The method for determination of phylogenetic relations among staphylococcus species strains

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL404497A PL226609B1 (en) 2013-06-28 2013-06-28 The use of polynucleotide, a set of primers and the method of determining the degree of relationship of Staphylococcus strains

Publications (2)

Publication Number Publication Date
PL404497A1 true PL404497A1 (en) 2015-01-05
PL226609B1 PL226609B1 (en) 2017-08-31

Family

ID=51293119

Family Applications (1)

Application Number Title Priority Date Filing Date
PL404497A PL226609B1 (en) 2013-06-28 2013-06-28 The use of polynucleotide, a set of primers and the method of determining the degree of relationship of Staphylococcus strains

Country Status (2)

Country Link
PL (1) PL226609B1 (en)
WO (1) WO2014209145A1 (en)

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AT503862B1 (en) * 2006-07-05 2010-11-15 Arc Austrian Res Centers Gmbh PATHOGENIC IDENTIFICATION DUE TO A 16S OR 18S RRNA MICROARRAY

Also Published As

Publication number Publication date
WO2014209145A1 (en) 2014-12-31
PL226609B1 (en) 2017-08-31

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