PL408917A1 - Method for producing the mixture of 2-methylbutyl estes of higher fatty acids - Google Patents
Method for producing the mixture of 2-methylbutyl estes of higher fatty acidsInfo
- Publication number
- PL408917A1 PL408917A1 PL408917A PL40891714A PL408917A1 PL 408917 A1 PL408917 A1 PL 408917A1 PL 408917 A PL408917 A PL 408917A PL 40891714 A PL40891714 A PL 40891714A PL 408917 A1 PL408917 A1 PL 408917A1
- Authority
- PL
- Poland
- Prior art keywords
- mycelium
- petroleum ether
- mold
- tza45
- circinelloides
- Prior art date
Links
- 125000004493 2-methylbut-1-yl group Chemical group CC(C*)CC 0.000 title abstract 2
- 235000014113 dietary fatty acids Nutrition 0.000 title abstract 2
- 239000000194 fatty acid Substances 0.000 title abstract 2
- 229930195729 fatty acid Natural products 0.000 title abstract 2
- 150000004665 fatty acids Chemical class 0.000 title abstract 2
- 239000000203 mixture Substances 0.000 title abstract 2
- 238000004519 manufacturing process Methods 0.000 title 1
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 abstract 8
- 241000306281 Mucor ambiguus Species 0.000 abstract 4
- 239000003208 petroleum Substances 0.000 abstract 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 4
- QPRQEDXDYOZYLA-UHFFFAOYSA-N 2-methylbutan-1-ol Chemical compound CCC(C)CO QPRQEDXDYOZYLA-UHFFFAOYSA-N 0.000 abstract 3
- 229920005830 Polyurethane Foam Polymers 0.000 abstract 3
- 238000000034 method Methods 0.000 abstract 3
- 239000011496 polyurethane foam Substances 0.000 abstract 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 abstract 2
- 102000004190 Enzymes Human genes 0.000 abstract 2
- 108090000790 Enzymes Proteins 0.000 abstract 2
- 238000006136 alcoholysis reaction Methods 0.000 abstract 2
- 239000011942 biocatalyst Substances 0.000 abstract 2
- 239000012153 distilled water Substances 0.000 abstract 2
- 239000006260 foam Substances 0.000 abstract 2
- 239000002609 medium Substances 0.000 abstract 2
- 239000013587 production medium Substances 0.000 abstract 2
- 239000007787 solid Substances 0.000 abstract 2
- 229920001817 Agar Polymers 0.000 abstract 1
- 235000019737 Animal fat Nutrition 0.000 abstract 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 abstract 1
- 241000235395 Mucor Species 0.000 abstract 1
- 235000019484 Rapeseed oil Nutrition 0.000 abstract 1
- 235000019486 Sunflower oil Nutrition 0.000 abstract 1
- 240000008042 Zea mays Species 0.000 abstract 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 abstract 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 abstract 1
- 230000004913 activation Effects 0.000 abstract 1
- 239000008272 agar Substances 0.000 abstract 1
- 235000010419 agar Nutrition 0.000 abstract 1
- 238000006243 chemical reaction Methods 0.000 abstract 1
- 235000005822 corn Nutrition 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 239000007788 liquid Substances 0.000 abstract 1
- 239000003921 oil Substances 0.000 abstract 1
- 235000019198 oils Nutrition 0.000 abstract 1
- 238000002360 preparation method Methods 0.000 abstract 1
- 239000012429 reaction media Substances 0.000 abstract 1
- 239000011541 reaction mixture Substances 0.000 abstract 1
- 238000000926 separation method Methods 0.000 abstract 1
- 239000002600 sunflower oil Substances 0.000 abstract 1
- 239000008399 tap water Substances 0.000 abstract 1
- 235000020679 tap water Nutrition 0.000 abstract 1
- 235000019871 vegetable fat Nutrition 0.000 abstract 1
- 238000005406 washing Methods 0.000 abstract 1
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Sposób wytwarzania mieszaniny 2-metylobutylowych estrów wyższych kwasów tłuszczowych, na drodze alkoholizy oleju słonecznikowego 2-metylobutan-1-olem, ewentualnie w środowisku eteru naftowego w obecności biokatalizatora w postaci odwodnionej i odlipidowanej grzybni selektanta pleśni Mucor circinelloides, którą otrzymuje się ze szczepu pleśni Mucor circinelloides wyodrębnionego ze środowiska naturalnego, w drodze poddania go aktywacji na wysterylizowanym i wychłodzonym podłożu stałym zawierającym tłuszcz prosty roślinny lub zwierzęcy, agar i brzeczkę piwowarską, następnie zmycia zarodników z tego podłoża i zaszczepienia tymi zarodnikami wysterylizowanego podłoża produkcyjnego, prowadzenia hodowli wstrząsanej, ewentualnie w obecności pianki poliuretanowej o całkowicie otwartej strukturze komórkowej, oddzielenia otrzymanego preparatu grzybni lub pianki z zaadsorbowaną grzybnią od cieczy pohodowlanej, z których piankę z zaadsorbowaną grzybnią przemywa się wodą destylowaną i suszy, zaś grzybnię wolną, przemytą wodą destylowaną, kilkakrotnie przemywa się acetonem i suszy, charakteryzuje się tym, że jako biokatalizator stosuje się grzybnię selektanta pleśni Mucor circinelloides TZA45, którą otrzymuje się w drodze hodowli na podłożu produkcyjnym zawierającym olej rzepakowy, namok kukurydziany, wodę wodociągową oraz ewentualnie pianki poliuretanowe. Proces alkoholizy prowadzi się w reaktorze periodycznym w obecności wolnej grzybni selektanta pleśni Mucor circinelloides TZA45, ewentualnie w środowisku eteru naftowego użytego w nadmiarze, korzystnie w obecności wody i po zakończeniu reakcji z mieszaniny reakcyjnej oddziela się części stałe grzybni, lub proces alkoholizy prowadzi się w reaktorze kolumnowym wypełnionym grzybnią selektanta pleśni Mucor circinelloides TZA45 immobilizowaną w piance poliuretanowej, przez którą najpierw przepuszcza się eter naftowy, a następnie, z recyrkulacją, medium reakcyjne zawierające olej, alkohol, eter naftowy i korzystnie wodę.A method of producing a mixture of 2-methylbutyl esters of higher fatty acids by alcoholysing sunflower oil with 2-methylbutan-1-ol, optionally in a petroleum ether environment in the presence of a biocatalyst in the form of dehydrated and delipidated mycelium of the Mucor circinelloides mold selector, which is obtained from the Mucor mold strain circinelloides extracted from the natural environment by activation on a sterilized and cooled solid medium containing simple vegetable or animal fat, agar and brewing wort, then washing the spores from this medium and inoculating the sterilized production medium with these spores, conducting a shake culture, possibly in the presence of polyurethane foam with a completely open cell structure, separation of the obtained mycelium preparation or foam with adsorbed mycelium from the post-culture liquid, from which the foam with adsorbed mycelium is washed with distilled water and dried, and The slow tank, washed with distilled water, washed several times with acetone and dried, is characterized in that the biocatalyst is the mycelium of the mold selector Mucor circinelloides TZA45, which is obtained by culturing on a production medium containing rapeseed oil, corn liquor, tap water and possibly polyurethane foams. The alcoholysis process is carried out in a batch reactor in the presence of free mycelium of the mold selector Mucor circinelloides TZA45, optionally in the environment of petroleum ether used in excess, preferably in the presence of water, and after the reaction is completed, solid parts of the mycelium are separated from the reaction mixture, or the alcoholysis process is carried out in a reactor column filled with mycelia of the mold selector Mucor circinelloides TZA45 immobilized in a polyurethane foam through which petroleum ether is first passed and then, with recirculation, a reaction medium containing oil, alcohol, petroleum ether and preferably water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PL408917A PL228104B1 (en) | 2014-07-18 | 2014-07-18 | Method for producing the mixture of 2-methylbutyl estes of higher fatty acids |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PL408917A PL228104B1 (en) | 2014-07-18 | 2014-07-18 | Method for producing the mixture of 2-methylbutyl estes of higher fatty acids |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| PL408917A1 true PL408917A1 (en) | 2016-02-01 |
| PL228104B1 PL228104B1 (en) | 2018-02-28 |
Family
ID=55178378
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PL408917A PL228104B1 (en) | 2014-07-18 | 2014-07-18 | Method for producing the mixture of 2-methylbutyl estes of higher fatty acids |
Country Status (1)
| Country | Link |
|---|---|
| PL (1) | PL228104B1 (en) |
-
2014
- 2014-07-18 PL PL408917A patent/PL228104B1/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| PL228104B1 (en) | 2018-02-28 |
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