PL410286A1 - Nowa metoda oczyszczania chitynazy A przy użyciu chromatografii powinowactwa - Google Patents

Nowa metoda oczyszczania chitynazy A przy użyciu chromatografii powinowactwa

Info

Publication number
PL410286A1
PL410286A1 PL410286A PL41028614A PL410286A1 PL 410286 A1 PL410286 A1 PL 410286A1 PL 410286 A PL410286 A PL 410286A PL 41028614 A PL41028614 A PL 41028614A PL 410286 A1 PL410286 A1 PL 410286A1
Authority
PL
Poland
Prior art keywords
chitinase
purification
protein
affinity chromatography
new method
Prior art date
Application number
PL410286A
Other languages
English (en)
Other versions
PL235436B1 (pl
Inventor
Joanna Bereta
Jarosław Jucha
Original Assignee
Uniwersytet Jagielloński
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Uniwersytet Jagielloński filed Critical Uniwersytet Jagielloński
Priority to PL410286A priority Critical patent/PL235436B1/pl
Priority to PCT/IB2015/056710 priority patent/WO2016083911A1/en
Publication of PL410286A1 publication Critical patent/PL410286A1/pl
Publication of PL235436B1 publication Critical patent/PL235436B1/pl

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2442—Chitinase (3.2.1.14)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/315—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01014—Chitinase (3.2.1.14)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Ujawniono kompleks białkowy zawierający białko G i związaną z nim niekowalencyjnie chitynazę A lub białko zawierające fragment chitynazy A mający powinowactwo do białka G oraz sposób wyodrębniania z roztworu wodnego chitynazy A lub białka zawierającego jej fragment mający powinowactwo do białka G, a także zastosowanie sposobu.
PL410286A 2014-11-28 2014-11-28 Kompleks białkowy zawierający białko G i chitynazę A, sposób wyodrębniania z roztworu wodnego chitynazy A oraz jego zastosowania PL235436B1 (pl)

Priority Applications (2)

Application Number Priority Date Filing Date Title
PL410286A PL235436B1 (pl) 2014-11-28 2014-11-28 Kompleks białkowy zawierający białko G i chitynazę A, sposób wyodrębniania z roztworu wodnego chitynazy A oraz jego zastosowania
PCT/IB2015/056710 WO2016083911A1 (en) 2014-11-28 2015-09-03 A novel method of chitinase a purification by affinity chromatography

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL410286A PL235436B1 (pl) 2014-11-28 2014-11-28 Kompleks białkowy zawierający białko G i chitynazę A, sposób wyodrębniania z roztworu wodnego chitynazy A oraz jego zastosowania

Publications (2)

Publication Number Publication Date
PL410286A1 true PL410286A1 (pl) 2016-06-06
PL235436B1 PL235436B1 (pl) 2020-08-10

Family

ID=54325568

Family Applications (1)

Application Number Title Priority Date Filing Date
PL410286A PL235436B1 (pl) 2014-11-28 2014-11-28 Kompleks białkowy zawierający białko G i chitynazę A, sposób wyodrębniania z roztworu wodnego chitynazy A oraz jego zastosowania

Country Status (2)

Country Link
PL (1) PL235436B1 (pl)
WO (1) WO2016083911A1 (pl)

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3862007A (en) * 1973-03-15 1975-01-21 Canadian Patents Dev Extraction of chitinase
US20030017570A1 (en) 1996-06-14 2003-01-23 Patrick W. Gray Chitinase materials and methods
JP3118573B1 (ja) 1999-10-27 2000-12-18 工業技術院長 キチナーゼ及びその製造法

Also Published As

Publication number Publication date
WO2016083911A1 (en) 2016-06-02
PL235436B1 (pl) 2020-08-10

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