PL413910A1 - Insert and method for separation of fluids, using the density gradient - Google Patents

Insert and method for separation of fluids, using the density gradient

Info

Publication number
PL413910A1
PL413910A1 PL413910A PL41391015A PL413910A1 PL 413910 A1 PL413910 A1 PL 413910A1 PL 413910 A PL413910 A PL 413910A PL 41391015 A PL41391015 A PL 41391015A PL 413910 A1 PL413910 A1 PL 413910A1
Authority
PL
Poland
Prior art keywords
insert
container
fluids
separation
density gradient
Prior art date
Application number
PL413910A
Other languages
Polish (pl)
Other versions
PL237582B1 (en
Inventor
Mateusz Grzegorz Adamski
Patryk Gumann
Original Assignee
Spark-Tech Spółka Z Ograniczoną Odpowiedzialnością
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Spark-Tech Spółka Z Ograniczoną Odpowiedzialnością filed Critical Spark-Tech Spółka Z Ograniczoną Odpowiedzialnością
Priority to PL413910A priority Critical patent/PL237582B1/en
Priority to CN201680065865.3A priority patent/CN108367288A/en
Priority to PCT/IB2016/055503 priority patent/WO2017046736A1/en
Priority to EP16791676.6A priority patent/EP3349897B8/en
Priority to US15/759,191 priority patent/US20180250669A1/en
Priority to ES16791676T priority patent/ES2910923T3/en
Priority to DK16791676.6T priority patent/DK3349897T3/en
Publication of PL413910A1 publication Critical patent/PL413910A1/en
Publication of PL237582B1 publication Critical patent/PL237582B1/en
Priority to US17/354,735 priority patent/US20210316299A1/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5021—Test tubes specially adapted for centrifugation purposes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/16—Reagents, handling or storing thereof
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0609—Holders integrated in container to position an object
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/087—Multiple sequential chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0633—Valves, specific forms thereof with moving parts
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0633—Valves, specific forms thereof with moving parts
    • B01L2400/0644—Valves, specific forms thereof with moving parts rotary valves

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Centrifugal Separators (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • External Artificial Organs (AREA)

Abstract

Wynalazek dotyczy insertu (6) do pojemnika (1) do wirowania, zwłaszcza do probówki, do wydzielenia frakcji o pożądanym zakresie gęstości metodą wirowania na gradiencie gęstości charakteryzującego się tym, że zawiera przegrodę (7), zasadniczo w kształcie spłaszczonego krążka, rozdzielającą wnętrze pojemnika (1) na dwie komory - komorę dolną (3) oraz komorę górną (2), przy czym przegroda (7) zawiera przynajmniej jedno wycięcie (4) usytuowane w pobliżu wewnętrznej ściany pojemnika (1) o takiej wielkości, które umożliwia przepływ płynów z komory górnej (2) do komory dolnej (3) po wewnętrznej ściance pojemnika, przy czym wycięcie (4) posiada zamknięcie. Zgłoszenie również dotyczy sposobu wydzielenia frakcji o pożądanym zakresie gęstości z próbki zawierającej frakcje o różnej gęstości, zwłaszcza z próbki biologicznej przy użyciu insertu (6). Zgłoszenie obejmuje także pojemnik do wirowania oraz zestaw zawierający insert (6).The invention relates to the insert (6) into the container (1) for centrifugation, in particular into the tube, for the separation of the fraction with the desired density range by density gradient centrifugation characterized in that it comprises a partition (7), essentially in the shape of a flattened disc, separating the interior of the container (1) for two chambers - the lower chamber (3) and the upper chamber (2), wherein the partition (7) contains at least one cutout (4) located near the inner wall of the container (1) of such a size that allows the flow of fluids from upper chamber (2) to the lower chamber (3) on the inner wall of the container, the notch (4) having a closure. The application also relates to a method of separating a fraction with a desired density range from a sample containing fractions of different density, especially from a biological sample using the insert (6). The notification also includes a spin container and a kit containing the insert (6).

PL413910A 2015-09-15 2015-09-15 Insert and method for separation of fluids, using the density gradient PL237582B1 (en)

Priority Applications (8)

Application Number Priority Date Filing Date Title
PL413910A PL237582B1 (en) 2015-09-15 2015-09-15 Insert and method for separation of fluids, using the density gradient
CN201680065865.3A CN108367288A (en) 2015-09-15 2016-09-15 The device and method for carrying out fluid separation by density gradient
PCT/IB2016/055503 WO2017046736A1 (en) 2015-09-15 2016-09-15 Device and method for fluids separation by density gradient
EP16791676.6A EP3349897B8 (en) 2015-09-15 2016-09-15 Device and method for fluid separation by density gradient centrifugation
US15/759,191 US20180250669A1 (en) 2015-09-15 2016-09-15 Device and method for fluids separation by density gradient
ES16791676T ES2910923T3 (en) 2015-09-15 2016-09-15 Device and method for fluid separation by density gradient centrifugation
DK16791676.6T DK3349897T3 (en) 2015-09-15 2016-09-15 DEVICE AND METHOD FOR LIQUID SEPARATION BY DENSITY GRADIENT CENTRIFUGATION
US17/354,735 US20210316299A1 (en) 2015-09-15 2021-06-22 Device and method for fluids separation by density gradient

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL413910A PL237582B1 (en) 2015-09-15 2015-09-15 Insert and method for separation of fluids, using the density gradient

Publications (2)

Publication Number Publication Date
PL413910A1 true PL413910A1 (en) 2017-03-27
PL237582B1 PL237582B1 (en) 2021-05-04

Family

ID=58360192

Family Applications (1)

Application Number Title Priority Date Filing Date
PL413910A PL237582B1 (en) 2015-09-15 2015-09-15 Insert and method for separation of fluids, using the density gradient

Country Status (5)

Country Link
EP (1) EP3349897B8 (en)
CN (1) CN108367288A (en)
DK (1) DK3349897T3 (en)
ES (1) ES2910923T3 (en)
PL (1) PL237582B1 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020117610A1 (en) * 2018-12-08 2020-06-11 Min Wei Apparatus for manufacturing cell therapy product
TWI683702B (en) * 2019-01-31 2020-02-01 瑩芳有限公司 Specimen container
SE545603C2 (en) * 2019-08-22 2023-11-07 Grimaldi Dev Ab Separating particles through centrifugal sedimentation
PL434517A1 (en) * 2020-06-30 2022-01-03 Spark-Tech Spółka Z Ograniczoną Odpowiedzialnością Device for the controlled layering of liquid pouring
CN113358451A (en) * 2021-03-30 2021-09-07 刘开 Novel centrifugal chromatography extractor
CN114053986B (en) * 2021-12-09 2024-12-10 杭州职业技术学院 Pre-packaged reagent containers for centrifugally controlled sequential mixing reactions

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5132232A (en) * 1985-07-30 1992-07-21 V-Tech, Inc. Method and apparatus for preparation of liquids for examination
IL100828A (en) * 1992-01-31 2002-05-23 Novamed Ltd Method and means for density gradient centrifugation
US5648223A (en) * 1994-08-31 1997-07-15 Activated Cell Therapy, Inc. Methods for enriching breast tumor cells
US6516953B1 (en) * 1998-12-05 2003-02-11 Becton, Dickinson And Company Device for separating components of a fluid sample
US7074577B2 (en) * 2002-10-03 2006-07-11 Battelle Memorial Institute Buffy coat tube and float system and method
JP4853295B2 (en) * 2007-01-11 2012-01-11 株式会社島津製作所 Centrifuge tube
BR112013028522B8 (en) * 2011-05-05 2021-03-30 Stemcell Tech Inc insert for a centrifuge tube, centrifuge tube comprising the same, method for separating a target population of cells from a sample and kit

Also Published As

Publication number Publication date
EP3349897B8 (en) 2022-02-16
EP3349897B1 (en) 2022-01-26
DK3349897T3 (en) 2022-04-04
CN108367288A (en) 2018-08-03
EP3349897A1 (en) 2018-07-25
PL237582B1 (en) 2021-05-04
ES2910923T3 (en) 2022-05-17

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