PL415883A1 - RNazy MiniIII, sposoby zmiany specyficzności cięcia sekwencji RNA przez RNazy MiniIII oraz ich zastosowania - Google Patents
RNazy MiniIII, sposoby zmiany specyficzności cięcia sekwencji RNA przez RNazy MiniIII oraz ich zastosowaniaInfo
- Publication number
- PL415883A1 PL415883A1 PL415883A PL41588316A PL415883A1 PL 415883 A1 PL415883 A1 PL 415883A1 PL 415883 A PL415883 A PL 415883A PL 41588316 A PL41588316 A PL 41588316A PL 415883 A1 PL415883 A1 PL 415883A1
- Authority
- PL
- Poland
- Prior art keywords
- miniiii
- rnase
- rnazy
- helix
- loop
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 3
- 108091028043 Nucleic acid sequence Proteins 0.000 title 1
- 102000006382 Ribonucleases Human genes 0.000 abstract 9
- 108010083644 Ribonucleases Proteins 0.000 abstract 9
- 108091032973 (ribonucleotides)n+m Proteins 0.000 abstract 3
- 102000040650 (ribonucleotides)n+m Human genes 0.000 abstract 3
- 108090000623 proteins and genes Proteins 0.000 abstract 3
- 244000063299 Bacillus subtilis Species 0.000 abstract 2
- 235000014469 Bacillus subtilis Nutrition 0.000 abstract 2
- 108091028664 Ribonucleotide Proteins 0.000 abstract 2
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 2
- 238000003776 cleavage reaction Methods 0.000 abstract 2
- 239000012634 fragment Substances 0.000 abstract 2
- 102000004169 proteins and genes Human genes 0.000 abstract 2
- 239000002336 ribonucleotide Substances 0.000 abstract 2
- 125000002652 ribonucleotide group Chemical group 0.000 abstract 2
- 230000007017 scission Effects 0.000 abstract 2
- 239000000758 substrate Substances 0.000 abstract 2
- 230000035772 mutation Effects 0.000 abstract 1
- 230000003319 supportive effect Effects 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/26—Endoribonucleases producing 5'-phosphomonoesters (3.1.26)
- C12Y301/26003—Ribonuclease III (3.1.26.3)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Przedmiotem zgłoszenia jest RNaza MiniIII, której sekwencja aminokwasowa obejmuje część akceptorową i przeszczepialną helisę ?4 oraz przeszczepialną pętlę ?5b - ?6, tworzące struktury odpowiednio helisy ?4 i pętli ?5b - ?6 w strukturze RNazy MiniIII, przy czym fragmenty tworzące struktury odpowiednio helisy ?4 i pętli ?5b - ?6 odpowiadają strukturalnie odpowiednim strukturom helisy ?4 i pętli ?5b- ?6, tworzonym przez fragmenty odpowiednio 46 - 52 i 85 - 98 sekwencji aminokwasowej RNazy MiniIII z Bacillus subtilis, przy czym wymieniona RNaza MiniIII wykazuje w cięciu dsRNA specyficzność sekwencyjną, zależną jedynie od sekwencji rybonukleotydowej substratu, a niezależną od występowania struktur drugorzędowych w strukturze substratu i niezależną od obecności innych białek wspomagających, i przy czym RNazą MiniIII nie jest białko MiniIII z Bacillus subtilis o SEQ ID NO: 1 ani o SEQ ID NO: 1 z mutacją D94R. Zgłoszenie dotyczy również sposobu otrzymywania chimerycznej RNazy MiniIII, konstruktu kodującego RNazę MiniIII, komórki obejmującej gen kodujący RNazę MiniIII, zastosowania RNazy MiniIII do cięcia dsRNA oraz sposobu cięcia dsRNA w sposób zależny jedynie od sekwencji rybonukleotydowej.
Priority Applications (9)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PL415883A PL415883A1 (pl) | 2016-01-22 | 2016-01-22 | RNazy MiniIII, sposoby zmiany specyficzności cięcia sekwencji RNA przez RNazy MiniIII oraz ich zastosowania |
| PCT/IB2017/050296 WO2017125880A1 (en) | 2016-01-22 | 2017-01-20 | Mini-ill rnases, methods for changing specificity of rna sequence cleavage by mini-ill rnases, and uses thereof |
| JP2018537846A JP2019502394A (ja) | 2016-01-22 | 2017-01-20 | Ｍｉｎｉ−ＩＩＩ ＲＮａｓｅ、Ｍｉｎｉ−ＩＩＩ ＲＮａｓｅによるＲＮＡ配列切断の特異性を変更する方法、およびそれらの使用 |
| CA3012221A CA3012221A1 (en) | 2016-01-22 | 2017-01-20 | Mini-iii rnases, methods for changing specificity of rna sequence cleavage by mini-iii rnases, and uses thereof |
| EP17719316.6A EP3405571A1 (en) | 2016-01-22 | 2017-01-20 | Mini-ill rnases, methods for changing specificity of rna sequence cleavage by mini-ill rnases, and uses thereof |
| KR1020187020889A KR20180100139A (ko) | 2016-01-22 | 2017-01-20 | 미니-ILL RNase, 미니-ILL RNase에 의한 RNA 서열 절단의 특이성을 변화시키는 방법, 및 이의 용도 |
| CN201780019412.1A CN108884450A (zh) | 2016-01-22 | 2017-01-20 | Mini-III RNA酶、改变Mini-III RNA酶切割RNA序列的特异性的方法及其用途 |
| AU2017208930A AU2017208930A1 (en) | 2016-01-22 | 2017-01-20 | Mini-ILL RNases, methods for changing specificity of RNA sequence cleavage by mini-ILL RNases, and uses thereof |
| US16/071,867 US20190032035A1 (en) | 2016-01-22 | 2017-07-20 | Mini-III RNases, Methods for Changing the Specificity of RNA Sequence Cleavage by Mini-III RNases, and Uses Thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PL415883A PL415883A1 (pl) | 2016-01-22 | 2016-01-22 | RNazy MiniIII, sposoby zmiany specyficzności cięcia sekwencji RNA przez RNazy MiniIII oraz ich zastosowania |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| PL415883A1 true PL415883A1 (pl) | 2017-07-31 |
Family
ID=58632539
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PL415883A PL415883A1 (pl) | 2016-01-22 | 2016-01-22 | RNazy MiniIII, sposoby zmiany specyficzności cięcia sekwencji RNA przez RNazy MiniIII oraz ich zastosowania |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US20190032035A1 (pl) |
| EP (1) | EP3405571A1 (pl) |
| JP (1) | JP2019502394A (pl) |
| KR (1) | KR20180100139A (pl) |
| CN (1) | CN108884450A (pl) |
| AU (1) | AU2017208930A1 (pl) |
| CA (1) | CA3012221A1 (pl) |
| PL (1) | PL415883A1 (pl) |
| WO (1) | WO2017125880A1 (pl) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111363056B (zh) | 2020-03-17 | 2021-07-09 | 湖南省植物保护研究所 | 沼泽红假单胞菌胞外多糖及其制备方法和应用 |
| CN114438054B (zh) | 2022-01-28 | 2023-03-28 | 广州吉赛生物科技股份有限公司 | 一种突变型RNase R及其制备方法和应用 |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| PL222511B1 (pl) * | 2011-06-08 | 2016-08-31 | Międzynarodowy Inst Biologii Molekularnej I Komórkowej W Warszawie | Endorybonukleazy dsRNA |
-
2016
- 2016-01-22 PL PL415883A patent/PL415883A1/pl unknown
-
2017
- 2017-01-20 KR KR1020187020889A patent/KR20180100139A/ko not_active Withdrawn
- 2017-01-20 CA CA3012221A patent/CA3012221A1/en not_active Abandoned
- 2017-01-20 AU AU2017208930A patent/AU2017208930A1/en not_active Abandoned
- 2017-01-20 JP JP2018537846A patent/JP2019502394A/ja active Pending
- 2017-01-20 WO PCT/IB2017/050296 patent/WO2017125880A1/en not_active Ceased
- 2017-01-20 EP EP17719316.6A patent/EP3405571A1/en not_active Withdrawn
- 2017-01-20 CN CN201780019412.1A patent/CN108884450A/zh active Pending
- 2017-07-20 US US16/071,867 patent/US20190032035A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| CA3012221A1 (en) | 2017-07-27 |
| JP2019502394A (ja) | 2019-01-31 |
| EP3405571A1 (en) | 2018-11-28 |
| CN108884450A (zh) | 2018-11-23 |
| AU2017208930A1 (en) | 2018-08-02 |
| WO2017125880A1 (en) | 2017-07-27 |
| KR20180100139A (ko) | 2018-09-07 |
| US20190032035A1 (en) | 2019-01-31 |
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