PL419488A1 - Oligonucleotide primers for detecting wheat fungal pathogen Blumeria graminis and method for detecting it - Google Patents
Oligonucleotide primers for detecting wheat fungal pathogen Blumeria graminis and method for detecting itInfo
- Publication number
- PL419488A1 PL419488A1 PL419488A PL41948816A PL419488A1 PL 419488 A1 PL419488 A1 PL 419488A1 PL 419488 A PL419488 A PL 419488A PL 41948816 A PL41948816 A PL 41948816A PL 419488 A1 PL419488 A1 PL 419488A1
- Authority
- PL
- Poland
- Prior art keywords
- detecting
- oligonucleotide primers
- fungal pathogen
- blumeria graminis
- application
- Prior art date
Links
- 241001480061 Blumeria graminis Species 0.000 title abstract 3
- 239000003155 DNA primer Substances 0.000 title abstract 3
- 241000209140 Triticum Species 0.000 title abstract 2
- 235000021307 Triticum Nutrition 0.000 title abstract 2
- 238000000034 method Methods 0.000 title abstract 2
- 238000012986 modification Methods 0.000 abstract 2
- 230000004048 modification Effects 0.000 abstract 2
- 238000003752 polymerase chain reaction Methods 0.000 abstract 2
- 241000894007 species Species 0.000 abstract 2
- 108091034117 Oligonucleotide Proteins 0.000 abstract 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 abstract 1
- 239000012620 biological material Substances 0.000 abstract 1
- 230000000295 complement effect Effects 0.000 abstract 1
- 239000012634 fragment Substances 0.000 abstract 1
- 244000053095 fungal pathogen Species 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Zgłoszenie dotyczy oligonukleotydów, umożliwiających stwierdzanie obecności materiału genetycznego patogenu grzybowego Blumeria graminis w badanym materiale biologicznym z wykorzystaniem łańcuchowej reakcji polimerazy lub jej modyfikacji. Przedmiot zgłoszenia stanowi para specyficznych gatunkowo starterów oligonukleotydowych do zastosowania w reakcji łańcuchowej polimerazy lub jej modyfikacji, o sekwencjach: LidBg21: 5'-AATTCGGCTTTAGCATTGCGTT-3' LidBg22: 5'-TTCGTGTTCCCCAGAATATATCA-3' komplementarnych do specyficznych gatunkowo fragmentów sekwencji genomu Blumeria graminis. Zgłoszenie obejmuje też sposób wykrywania patogenu grzybowego pszenicy Blumeria graminis, w którym dochodzi do amplifikacji określonego fragmentu DNA o długości 220 - 260 par zasad w reakcji PCR z zastosowaniem pary starterów oligonukleotydowych.The application relates to oligonucleotides enabling the presence of the genetic material of the fungal pathogen Blumeria graminis to be determined in the biological material tested using the polymerase chain reaction or its modification. The subject of the application is a pair of species-specific oligonucleotide primers for use in the polymerase chain reaction or its modification, with the sequences: LidBg21: 5'-AATTCGGCTTTAGCATTGCGTT-3 'LidBg22: 5'-TTCGTGTTCCCCAGAATATATCA-3' of species-specific complementary genomes. The application also includes a method of detecting the fungal pathogen of wheat Blumeria graminis, in which a specific fragment of DNA with a length of 220 - 260 base pairs is amplified in a PCR reaction using a pair of oligonucleotide primers.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PL419488A PL230742B1 (en) | 2016-11-17 | 2016-11-17 | Oligonucleotide primers for detecting wheat fungal pathogen Blumeria graminis and method for detecting it |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PL419488A PL230742B1 (en) | 2016-11-17 | 2016-11-17 | Oligonucleotide primers for detecting wheat fungal pathogen Blumeria graminis and method for detecting it |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| PL419488A1 true PL419488A1 (en) | 2018-05-21 |
| PL230742B1 PL230742B1 (en) | 2018-12-31 |
Family
ID=62142466
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PL419488A PL230742B1 (en) | 2016-11-17 | 2016-11-17 | Oligonucleotide primers for detecting wheat fungal pathogen Blumeria graminis and method for detecting it |
Country Status (1)
| Country | Link |
|---|---|
| PL (1) | PL230742B1 (en) |
-
2016
- 2016-11-17 PL PL419488A patent/PL230742B1/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| PL230742B1 (en) | 2018-12-31 |
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