PL421579A1 - Method for obtaining adventitious shoots of Astrophytum myriostigma 'Fukuryu Hakuyo' cactus, originated from the in-vitro cultures - Google Patents
Method for obtaining adventitious shoots of Astrophytum myriostigma 'Fukuryu Hakuyo' cactus, originated from the in-vitro culturesInfo
- Publication number
- PL421579A1 PL421579A1 PL421579A PL42157917A PL421579A1 PL 421579 A1 PL421579 A1 PL 421579A1 PL 421579 A PL421579 A PL 421579A PL 42157917 A PL42157917 A PL 42157917A PL 421579 A1 PL421579 A1 PL 421579A1
- Authority
- PL
- Poland
- Prior art keywords
- shoots
- vitro cultures
- minutes
- myriostigma
- fukuryu
- Prior art date
Links
- 238000000338 in vitro Methods 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 3
- 241000883965 Astrophytum myriostigma Species 0.000 title abstract 2
- 241000219357 Cactaceae Species 0.000 title 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 abstract 2
- 239000012153 distilled water Substances 0.000 abstract 2
- 229920001817 Agar Polymers 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 abstract 1
- 239000005708 Sodium hypochlorite Substances 0.000 abstract 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 abstract 1
- 229930006000 Sucrose Natural products 0.000 abstract 1
- 239000008272 agar Substances 0.000 abstract 1
- 239000003599 detergent Substances 0.000 abstract 1
- 239000000417 fungicide Substances 0.000 abstract 1
- 238000003898 horticulture Methods 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 239000006870 ms-medium Substances 0.000 abstract 1
- 238000002791 soaking Methods 0.000 abstract 1
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 abstract 1
- 239000005720 sucrose Substances 0.000 abstract 1
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
Przedmiotem zgłoszenia jest sposób otrzymywania pędów przybyszowych kaktusa Astrophytum myriostigma 'Fukuryu Hakuyo' pochodzących z kultur in vitro z przeznaczeniem do dalszej uprawy szklarniowej, upraw ogrodniczych, produkcji ogrodniczej lub upraw hobbystycznych. Sposób charakteryzuje się tym, że pędy rośliny matecznej sterylizuje poprzez moczenie 5 - 10 minut w wodzie destylowanej z dodatkiem 10 - 100 µl detergentu, kolejno płucze się pod bieżącą wodą, oczyszcza z włosków, moczy w środkach grzybobójczych przez 17 - 18 godzin, a następnie płucze w 60 - 80% etanolu oraz moczy w podchlorynie sodu o stężeniu 1 - 2% przez 20 - 40 minut, następnie płucze się 3 krotnie po 5 - 10 minut w sterylnej wodzie destylowanej i osusza na sterylnej bibule w komorze laminarnej, kolejno pędy tnie się skalpelem na plastry o grubości 1 - 10 mm i umieszcza w sposób polarny na pożywce MS z dodatkiem agaru w ilości 6 - 12 g • dm-3 oraz sacharozy w ilości 20 - 50 g • dm-3 o pH 5,2 - 6,0, następnie kultury in vitro ustawia się w pokoju wzrostowym w temperaturze 24°C ± 2°C i wilgotności względnej powietrza 60 - 80%, naświetlając lampami jarzeniowymi o świetle dziennym i natężeniu napromienienia kwantowego 20 - 60 µM • m-2 • s-1, przez 16 godzin dziennie.The subject of the notification is the method of obtaining adventitious shoots of the cactus Astrophytum myriostigma 'Fukuryu Hakuyo' derived from in vitro cultures for further greenhouse cultivation, horticulture, horticultural production or hobby cultivation. The method is characterized in that the shoots of the mother plant are sterilized by soaking 5 - 10 minutes in distilled water with the addition of 10 - 100 µl of detergent, then rinsed under running water, cleaned of hair, soaked in fungicides for 17 - 18 hours, and then washed in 60 - 80% ethanol and soaked in 1 - 2% sodium hypochlorite for 20 - 40 minutes, then washed 3 times for 5 - 10 minutes in sterile distilled water and dried on sterile paper in a laminar chamber, successively cutting shoots using a scalpel for slices 1 - 10 mm thick and placed polarly on MS medium with the addition of agar in the amount of 6 - 12 g • dm-3 and sucrose in the amount of 20 - 50 g • dm-3 at pH 5.2 - 6 , 0, then in vitro cultures are set up in a growth room at 24 ° C ± 2 ° C and 60 - 80% relative humidity, illuminated with daylight fluorescent lamps and 20 - 60 µM quantum irradiance • m-2 • s-1, for 16 hours a day.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PL421579A PL233253B1 (en) | 2017-05-12 | 2017-05-12 | Method for obtaining adventitious shoots of Astrophytum myriostigma 'Fukuryu Hakuyo' cactus, originated from the in-vitro cultures |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PL421579A PL233253B1 (en) | 2017-05-12 | 2017-05-12 | Method for obtaining adventitious shoots of Astrophytum myriostigma 'Fukuryu Hakuyo' cactus, originated from the in-vitro cultures |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| PL421579A1 true PL421579A1 (en) | 2018-11-19 |
| PL233253B1 PL233253B1 (en) | 2019-09-30 |
Family
ID=64213738
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PL421579A PL233253B1 (en) | 2017-05-12 | 2017-05-12 | Method for obtaining adventitious shoots of Astrophytum myriostigma 'Fukuryu Hakuyo' cactus, originated from the in-vitro cultures |
Country Status (1)
| Country | Link |
|---|---|
| PL (1) | PL233253B1 (en) |
-
2017
- 2017-05-12 PL PL421579A patent/PL233253B1/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| PL233253B1 (en) | 2019-09-30 |
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