PL429362A1 - Nowa, niespecyficzna termolabilna nukleaza aktywna w niskich temperaturach i wysokich stężeniach soli - Google Patents

Nowa, niespecyficzna termolabilna nukleaza aktywna w niskich temperaturach i wysokich stężeniach soli

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Publication number
PL429362A1
PL429362A1 PL429362A PL42936219A PL429362A1 PL 429362 A1 PL429362 A1 PL 429362A1 PL 429362 A PL429362 A PL 429362A PL 42936219 A PL42936219 A PL 42936219A PL 429362 A1 PL429362 A1 PL 429362A1
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PL
Poland
Prior art keywords
mvi
nuclease
thermolabile
active fragment
enzymatically active
Prior art date
Application number
PL429362A
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English (en)
Inventor
Marcin Olszewski
Rafał Walkusz
Wioleta Lademan
Jan Balsewicz
Arkadiusz Popinigis
Dominik Ziętkowski
Krzysztof Kur
Robert Brodzik
Original Assignee
Blirt Spółka Akcyjna
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Blirt Spółka Akcyjna filed Critical Blirt Spółka Akcyjna
Priority to PL429362A priority Critical patent/PL429362A1/pl
Publication of PL429362A1 publication Critical patent/PL429362A1/pl

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/21—Endodeoxyribonucleases producing 5'-phosphomonoesters (3.1.21)
    • C12Y301/21001—Deoxyribonuclease I (3.1.21.1)

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

Przedmiotem wynalazku jest nowa termolabilna nukleaza Mvi lub jej enzymatycznie aktywny fragment, gdzie nukleaza ma sekwencję SEQ.Nr 2 lub sekwencję, która jest co najmniej w 40% z nią identyczna. Przedmiotem wynalazku jest gen kodujący nukleazę Mvi lub jej enzymatycznie aktywny fragment posiadający; cząsteczka kwasu nukleinowego kodująca nukleazę Mvi lub jej enzymatycznie aktywny fragment; plazmid ekspresyjny zawierający sekwencję genu kodującego Mvi; Rekombinantowy szczep Escherichia coli BL21 (DE3)-RlL pD454-Mvi; sposób wytwarzania białka nukleazy Mvi; zastosowanie nukleazy Mvi w procesach oczyszczania białek rekombinantowych o znacząco obniżonej zawartości DNA oraz do dekontaminacji reakcji PCR i RT PCR dla uzyskana zwiększonej czułości i specyficzności odpowiednich analiz genetycznych; Zastosowanie nukleazy Mvi w procesach oczyszczania białek rekombinantowych, w szczególności enzymów, przeciwciał, antygenów szczepionkowych, przygotowania preparatów używanych w terapiach komórkowych, i innych białkach terapeutycznych.
PL429362A 2019-03-22 2019-03-22 Nowa, niespecyficzna termolabilna nukleaza aktywna w niskich temperaturach i wysokich stężeniach soli PL429362A1 (pl)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PL429362A PL429362A1 (pl) 2019-03-22 2019-03-22 Nowa, niespecyficzna termolabilna nukleaza aktywna w niskich temperaturach i wysokich stężeniach soli

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL429362A PL429362A1 (pl) 2019-03-22 2019-03-22 Nowa, niespecyficzna termolabilna nukleaza aktywna w niskich temperaturach i wysokich stężeniach soli

Publications (1)

Publication Number Publication Date
PL429362A1 true PL429362A1 (pl) 2020-10-05

Family

ID=72669246

Family Applications (1)

Application Number Title Priority Date Filing Date
PL429362A PL429362A1 (pl) 2019-03-22 2019-03-22 Nowa, niespecyficzna termolabilna nukleaza aktywna w niskich temperaturach i wysokich stężeniach soli

Country Status (1)

Country Link
PL (1) PL429362A1 (pl)

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