PL431160A1 - Oligonucleotide primers hybridizing within the SdhB gene for detecting the wheat fungal pathogen Zymoseptoria tritici causing septoria leaf blotch, and method of its detection - Google Patents
Oligonucleotide primers hybridizing within the SdhB gene for detecting the wheat fungal pathogen Zymoseptoria tritici causing septoria leaf blotch, and method of its detectionInfo
- Publication number
- PL431160A1 PL431160A1 PL431160A PL43116019A PL431160A1 PL 431160 A1 PL431160 A1 PL 431160A1 PL 431160 A PL431160 A PL 431160A PL 43116019 A PL43116019 A PL 43116019A PL 431160 A1 PL431160 A1 PL 431160A1
- Authority
- PL
- Poland
- Prior art keywords
- detection
- oligonucleotide primers
- detecting
- fungal pathogen
- causing
- Prior art date
Links
- 239000003155 DNA primer Substances 0.000 title abstract 3
- 241000209140 Triticum Species 0.000 title abstract 3
- 235000021307 Triticum Nutrition 0.000 title abstract 3
- 241001360088 Zymoseptoria tritici Species 0.000 title abstract 3
- 238000001514 detection method Methods 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 2
- 101150108347 sdhB gene Proteins 0.000 title abstract 2
- 241001533598 Septoria Species 0.000 title 1
- 239000013615 primer Substances 0.000 abstract 2
- 239000012634 fragment Substances 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Immunology (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Botany (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Przedmiotem zgłoszenia są startery oligonukleotydowe hybrydyzujące w obrębie genu SdhB do wykrywania patogenu grzybowego pszenicy Zymoseptoria tritici o sekwencjach: LidSt15 5'-TGCACCATCCTTCACCTTCC-3' LidSt16 5'-CGAGTCGTTGATCCATCGGT-3'. Zgłoszenie obejmuje też sposób wykrywania patogenu grzybowego pszenicy Zymoseptoria tritici, w którym w reakcji PCR z zastosowaniem pary starterów dochodzi do amplifikacji określonego fragmentu DNA, po czym dokonuje się detekcji powstałego produktu, charakteryzujący się tym, że parę starterów stanowią startery oligonukleotydowe.The subject of the application are oligonucleotide primers hybridizing within the SdhB gene for the detection of the wheat fungal pathogen Zymoseptoria tritici with the sequences: LidSt15 5'-TGCACCATCCTTCACCTTCC-3 'LidSt16 5'-CGAGTCGTTGATCCATCGGT-3'. The application also covers a method for detecting the wheat fungal pathogen Zymoseptoria tritici, in which a specific DNA fragment is amplified by PCR using a pair of primers, followed by detection of the resulting product, characterized in that the primer pair is oligonucleotide primers.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PL431160A PL238697B1 (en) | 2019-09-16 | 2019-09-16 | Oligonucleotide primers hybridizing within the SdhB gene for the detection of the wheat fungal pathogen Zymoseptoria tritici causing leaf striped septoriosis and its detection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PL431160A PL238697B1 (en) | 2019-09-16 | 2019-09-16 | Oligonucleotide primers hybridizing within the SdhB gene for the detection of the wheat fungal pathogen Zymoseptoria tritici causing leaf striped septoriosis and its detection |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| PL431160A1 true PL431160A1 (en) | 2021-03-22 |
| PL238697B1 PL238697B1 (en) | 2021-09-27 |
Family
ID=75107933
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PL431160A PL238697B1 (en) | 2019-09-16 | 2019-09-16 | Oligonucleotide primers hybridizing within the SdhB gene for the detection of the wheat fungal pathogen Zymoseptoria tritici causing leaf striped septoriosis and its detection |
Country Status (1)
| Country | Link |
|---|---|
| PL (1) | PL238697B1 (en) |
-
2019
- 2019-09-16 PL PL431160A patent/PL238697B1/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| PL238697B1 (en) | 2021-09-27 |
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