PL438431A1 - Peptidoglycan hydrolase, compositions comprising it, uses thereof and method of hydrolysis employing the same - Google Patents

Peptidoglycan hydrolase, compositions comprising it, uses thereof and method of hydrolysis employing the same

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Publication number
PL438431A1
PL438431A1 PL438431A PL43843121A PL438431A1 PL 438431 A1 PL438431 A1 PL 438431A1 PL 438431 A PL438431 A PL 438431A PL 43843121 A PL43843121 A PL 43843121A PL 438431 A1 PL438431 A1 PL 438431A1
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PL
Poland
Prior art keywords
peptidoglycan
glycine
compositions
same
cross
Prior art date
Application number
PL438431A
Other languages
Polish (pl)
Other versions
PL243304B1 (en
Inventor
Izabela SABAŁA
Piotr Henryk MAŁECKI
Karolina TROCHIMIAK
Elżbieta JAGIELSKA
Weronika AUGUSTYNIAK
Original Assignee
Międzynarodowy Instytut Biologii Molekularnej i Komórkowej w Warszawie
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Międzynarodowy Instytut Biologii Molekularnej i Komórkowej w Warszawie filed Critical Międzynarodowy Instytut Biologii Molekularnej i Komórkowej w Warszawie
Priority to PL438431A priority Critical patent/PL243304B1/en
Priority to PCT/PL2022/050043 priority patent/WO2023282776A1/en
Priority to EP22838102.6A priority patent/EP4367230A4/en
Publication of PL438431A1 publication Critical patent/PL438431A1/en
Publication of PL243304B1 publication Critical patent/PL243304B1/en

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    • C—CHEMISTRY; METALLURGY
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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/50—Isolated enzymes; Isolated proteins
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P1/00—Disinfectants; Antimicrobial compounds or mixtures thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/46—Hydrolases (3)
    • A61K38/47—Hydrolases (3) acting on glycosyl compounds (3.2), e.g. cellulases, lactases
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
    • A61K8/66—Enzymes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2462—Lysozyme (3.2.1.17)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6402—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from non-mammals
    • C12N9/6405—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from non-mammals not being snakes
    • C12N9/6416—Metalloendopeptidases (3.4.24)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
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    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01017—Lysozyme (3.2.1.17)
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    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/24—Metalloendopeptidases (3.4.24)
    • C—CHEMISTRY; METALLURGY
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    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/24—Metalloendopeptidases (3.4.24)
    • C12Y304/24075—Lysostaphin (3.4.24.75)
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    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/01—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in linear amides (3.5.1)
    • C12Y305/01028—N-Acetylmuramoyl-L-alanine amidase (3.5.1.28)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/10—General cosmetic use
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
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  • Public Health (AREA)
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  • Pharmacology & Pharmacy (AREA)
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  • Birds (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
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  • Communicable Diseases (AREA)
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  • Physics & Mathematics (AREA)
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Abstract

Przedmiotem wynalazku jest aktywna forma AurR, będąca - hydrolazą peptydoglikanową - zdolną do trawienia ścian bakterii Gram(+) posiadających mostki sieciujące w peptydoglikanie zbudowane z glicyny i/lub glicyny i seryny, kompozycje ją obejmujące, ich zastosowania oraz sposób hydrolizy peptydoglikanów. Aktywna forma białka AurR, przełamuje oporność bakterii Gram(+) wobec znanych enzymów zdolnych jedynie do hydrolizy ścian komórkowych z glicynowymi mostkami sieciującymi w peptydoglikanie i trawi ściany komórkowe z mostkami sieciującymi glicynowo-serynowymi.The subject of the invention is the active form of AurR, which is a peptidoglycan hydrolase capable of digesting the walls of Gram(+) bacteria having glycine and/or glycine and serine cross-linking bridges in the peptidoglycan, compositions comprising it, their applications and a method of peptidoglycan hydrolysis. The active form of the AurR protein breaks the resistance of Gram(+) bacteria to known enzymes capable only of hydrolyzing cell walls with glycine cross-linking bridges in peptidoglycan and digests cell walls with glycine-serine cross-linking bridges.

PL438431A 2021-07-09 2021-07-09 Peptidoglycan hydrolase, compositions comprising it, uses thereof and a method of hydrolysis employing the same PL243304B1 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
PL438431A PL243304B1 (en) 2021-07-09 2021-07-09 Peptidoglycan hydrolase, compositions comprising it, uses thereof and a method of hydrolysis employing the same
PCT/PL2022/050043 WO2023282776A1 (en) 2021-07-09 2022-07-06 Peptidoglycan hydrolase, compositions comprising it, uses thereof, and a method of hydrolysis utilizing it
EP22838102.6A EP4367230A4 (en) 2021-07-09 2022-07-06 Peptidoglycan hydrolase, compositions comprising it, uses thereof, and a method of hydrolysis utilizing it

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL438431A PL243304B1 (en) 2021-07-09 2021-07-09 Peptidoglycan hydrolase, compositions comprising it, uses thereof and a method of hydrolysis employing the same

Publications (2)

Publication Number Publication Date
PL438431A1 true PL438431A1 (en) 2023-01-16
PL243304B1 PL243304B1 (en) 2023-07-31

Family

ID=84801892

Family Applications (1)

Application Number Title Priority Date Filing Date
PL438431A PL243304B1 (en) 2021-07-09 2021-07-09 Peptidoglycan hydrolase, compositions comprising it, uses thereof and a method of hydrolysis employing the same

Country Status (3)

Country Link
EP (1) EP4367230A4 (en)
PL (1) PL243304B1 (en)
WO (1) WO2023282776A1 (en)

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL394619A1 (en) 2011-04-19 2012-10-22 Miedzynarodowy Instytut Biologii Molekularnej I Komórkowej Method of proteolysis, peptidase, a composition for use as a bactericide or bacteriostatic agent, a kit and the use of an active form of S. aureus LytM or a derivative thereof
ES2665821T3 (en) * 2015-09-15 2018-04-27 Micreos Human Health B.V. New Endolysin Polypeptide
PL243303B1 (en) 2019-10-13 2023-07-31 Miedzynarodowy Inst Biologii Molekularnej I Komorkowej A recombinant polypeptide for use in therapy, compositions comprising it, and uses thereof

Also Published As

Publication number Publication date
WO2023282776A1 (en) 2023-01-12
PL243304B1 (en) 2023-07-31
EP4367230A1 (en) 2024-05-15
EP4367230A4 (en) 2025-05-14

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