PL439284A1 - Syntetyczny regulator ekspresji genów oraz sposób wytwarzania mieszańców roślin uprawnych z jego wykorzystaniem - Google Patents

Syntetyczny regulator ekspresji genów oraz sposób wytwarzania mieszańców roślin uprawnych z jego wykorzystaniem

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Publication number
PL439284A1
PL439284A1 PL439284A PL43928421A PL439284A1 PL 439284 A1 PL439284 A1 PL 439284A1 PL 439284 A PL439284 A PL 439284A PL 43928421 A PL43928421 A PL 43928421A PL 439284 A1 PL439284 A1 PL 439284A1
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PL
Poland
Prior art keywords
expression
protein
gene expression
synthetic gene
cultivated plants
Prior art date
Application number
PL439284A
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English (en)
Other versions
PL249598B1 (pl
Inventor
Leszek Aleksander ŁYŻNIK
Monika RAKOCZY-TROJANOWSKA
Magdalena ŚWIĘCICKA
Bartosz SZABAŁA
Original Assignee
Szkoła Główna Gospodarstwa Wiejskiego w Warszawie
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Application filed by Szkoła Główna Gospodarstwa Wiejskiego w Warszawie filed Critical Szkoła Główna Gospodarstwa Wiejskiego w Warszawie
Priority to PL439284A priority Critical patent/PL249598B1/pl
Priority to PCT/PL2022/050069 priority patent/WO2023068950A1/en
Priority to EP22829622.4A priority patent/EP4419662A1/en
Priority to US18/701,455 priority patent/US20250051791A1/en
Publication of PL439284A1 publication Critical patent/PL439284A1/pl
Publication of PL249598B1 publication Critical patent/PL249598B1/pl

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C12N15/8207—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
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    • C12N15/8231—Male-specific, e.g. anther, tapetum, pollen
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    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • A01H1/022—Genic fertility modification, e.g. apomixis
    • A01H1/023—Male sterility
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/12—Processes for modifying agronomic input traits, e.g. crop yield
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits

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  • Developmental Biology & Embryology (AREA)
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  • Pregnancy & Childbirth (AREA)
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  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
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Abstract

Przedmiotem zgłoszenia jest syntetyczny regulator ekspresji genu roślinnego związanego z fenotypową cechą męskiej sterylności zawierający białko posiadające białkową domenę wiążącą się z DNA genu roślinnego związanego z fenotypową cechą męskiej sterylności oraz połączoną z nią białkową domenę aktywatora lub represora procesu transkrypcji tego genu, przy czym białkowa domena wiążącą się z DNA tworzy kompleks z cząsteczką RNA posiadającą sekwencję komplementarną do elementu kontrolującego ekspresję genu sterylności. Ponadto, przedmiotem zgłoszenia jest także kaseta ekspresyjna kodująca regulator ekspresji, wektor ekspresyjny zawierający kasetę oraz sposób otrzymywania męsko-sterylnych roślin.
PL439284A 2021-10-22 2021-10-22 Syntetyczny regulator ekspresji genów, kodująca go kaseta ekspresyjna i wektor ekspresyjny ją zawierający oraz sposób otrzymywania męsko-sterylnych roślin z ich wykorzystaniem PL249598B1 (pl)

Priority Applications (4)

Application Number Priority Date Filing Date Title
PL439284A PL249598B1 (pl) 2021-10-22 2021-10-22 Syntetyczny regulator ekspresji genów, kodująca go kaseta ekspresyjna i wektor ekspresyjny ją zawierający oraz sposób otrzymywania męsko-sterylnych roślin z ich wykorzystaniem
PCT/PL2022/050069 WO2023068950A1 (en) 2021-10-22 2022-10-24 A synthetic gene expression regulator and a process for producing hybrid crops using thereof
EP22829622.4A EP4419662A1 (en) 2021-10-22 2022-10-24 A synthetic gene expression regulator and a process for producing hybrid crops using thereof
US18/701,455 US20250051791A1 (en) 2021-10-22 2022-10-24 A synthetic gene expression regulator and a process for producing hybrid crops using thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL439284A PL249598B1 (pl) 2021-10-22 2021-10-22 Syntetyczny regulator ekspresji genów, kodująca go kaseta ekspresyjna i wektor ekspresyjny ją zawierający oraz sposób otrzymywania męsko-sterylnych roślin z ich wykorzystaniem

Publications (2)

Publication Number Publication Date
PL439284A1 true PL439284A1 (pl) 2023-04-24
PL249598B1 PL249598B1 (pl) 2026-05-18

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PL439284A PL249598B1 (pl) 2021-10-22 2021-10-22 Syntetyczny regulator ekspresji genów, kodująca go kaseta ekspresyjna i wektor ekspresyjny ją zawierający oraz sposób otrzymywania męsko-sterylnych roślin z ich wykorzystaniem

Country Status (4)

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US (1) US20250051791A1 (pl)
EP (1) EP4419662A1 (pl)
PL (1) PL249598B1 (pl)
WO (1) WO2023068950A1 (pl)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN120272515A (zh) * 2025-04-03 2025-07-08 中国科学院植物研究所 一种莴苣LsNP1基因及其在制备雄性不育植物中的应用

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109402141B (zh) * 2015-06-04 2021-10-01 山东农业大学 小麦雄性不育基因wms及其花药特异性启动子的应用
WO2019118342A1 (en) * 2017-12-11 2019-06-20 Pioneer Hi-Bred International, Inc. Compositions and methods of modifying a plant genome to produce a ms1 or ms5 male-sterile plant

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Publication number Publication date
US20250051791A1 (en) 2025-02-13
EP4419662A1 (en) 2024-08-28
PL249598B1 (pl) 2026-05-18
WO2023068950A1 (en) 2023-04-27

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