PL441114A1 - Method for obtaining Saccharomyces cerevisiae yeast biomass with increased resistance to osmotic stress and use of biomass - Google Patents

Method for obtaining Saccharomyces cerevisiae yeast biomass with increased resistance to osmotic stress and use of biomass

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Publication number
PL441114A1
PL441114A1 PL441114A PL44111422A PL441114A1 PL 441114 A1 PL441114 A1 PL 441114A1 PL 441114 A PL441114 A PL 441114A PL 44111422 A PL44111422 A PL 44111422A PL 441114 A1 PL441114 A1 PL 441114A1
Authority
PL
Poland
Prior art keywords
sup
amount
biomass
saccharomyces cerevisiae
cerevisiae yeast
Prior art date
Application number
PL441114A
Other languages
Polish (pl)
Other versions
PL243313B1 (en
Inventor
Jarosław Kliks
Mateusz Ciepliński
Justyna Korycka-Korwek
Mariusz Kasprzak
Maria Mrówczyńska
Dominika Lachnicka
Original Assignee
Uniwersytet Zielonogórski
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Uniwersytet Zielonogórski filed Critical Uniwersytet Zielonogórski
Priority to PL441114A priority Critical patent/PL243313B1/en
Publication of PL441114A1 publication Critical patent/PL441114A1/en
Priority to EP22216642.3A priority patent/EP4273221A1/en
Publication of PL243313B1 publication Critical patent/PL243313B1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C12N1/185—Saccharomyces isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/14—Yeasts or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
    • C12N2500/14—Calcium; Ca chelators; Calcitonin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
    • C12N2500/16—Magnesium; Mg chelators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/22—Zinc; Zn chelators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/34—Sugars
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/36—Lipids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/85—Saccharomyces
    • C12R2001/865—Saccharomyces cerevisiae

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Mycology (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Botany (AREA)
  • Nutrition Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Przedmiotem wynalazku jest sposób otrzymywania biomasy drożdży Saccharomyces cerevisiae o zwiększonej odporności na stres osmotyczny, w którym do suchej masy drożdży w ilości 0,98% - 2,65% ogólnej masy przygotowywanej biomasy wlewa się wodę demineralizowaną w ilości 88,21 - 97,69% ogólnej masy przygotowywanej biomasy, zawierającą rozpuszczone jony wapnia w ilości 144 – 180 mg/dm<sup>3</sup>, potasu w ilości 286 – 716 mg/dm<sup>3</sup>, magnezu w ilości 100 - 160 mg/dm<sup>3</sup>, azotu amonowego w ilości 106 – 360 mg/dm<sup>3</sup>, cynku w ilości 45 – 68 mg/dm<sup>3</sup> oraz glukozę w ilości 9,80 — 35,30 g/dm<sup>3</sup>, miesza się przez 5 godzin z prędkością 130 obr./min w temperaturze 20°C, a następnie po 5 godzinach dodaje się do mieszaniny estry etylowe kwasów tłuszczowych w ilości od 0,1% do 5% na 1 dm<sup>3</sup> wody, a następnie kondycjonuje się biomasę mieszając składniki z prędkością 130 obr./min w ciśnieniu atmosferycznym od 300 do 800 mbar z udziałem tlenu. Przedmiotem wynalazku jest także zastosowanie biomasy drożdży Saccharomyces cerevisiae jako dodatek spożywczy, suplement diety oraz jako prekursor do otrzymywania biomasy fermentacyjnej o zwiększonych parametrach fermentacyjnych.The subject of the invention is a method of obtaining Saccharomyces cerevisiae yeast biomass with increased resistance to osmotic stress, in which demineralized water in the amount of 88.21 - 97.69 is poured into the yeast dry matter in the amount of 0.98% - 2.65% of the total mass of the prepared biomass. % of the total weight of the prepared biomass, containing dissolved calcium ions in the amount of 144 - 180 mg/dm<sup>3</sup>, potassium in the amount of 286 - 716 mg/dm<sup>3</sup>, magnesium in the amount of 100 - 160 mg/dm<sup>3</sup>, ammonium nitrogen in the amount of 106 – 360 mg/dm<sup>3</sup>, zinc in the amount of 45 – 68 mg/dm<sup>3</sup> and glucose in the amount of 9.80 - 35.30 g/dm<sup>3</sup>, is stirred for 5 hours at a speed of 130 rpm at 20°C, and then after 5 hours esters are added to the mixture of fatty acids in the amount of 0.1% to 5% per 1 dm<sup>3</sup> of water, and then the biomass is conditioned by mixing the ingredients at a speed of 130 rpm at atmospheric pressure of 300 to 800 mbar with oxygen. The subject of the invention is also the use of Saccharomyces cerevisiae yeast biomass as a food additive, dietary supplement and as a precursor for obtaining fermentation biomass with increased fermentation parameters.

PL441114A 2022-05-06 2022-05-06 Method for obtaining Saccharomyces cerevisiae yeast biomass with increased resistance to osmotic stress and use of biomass PL243313B1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
PL441114A PL243313B1 (en) 2022-05-06 2022-05-06 Method for obtaining Saccharomyces cerevisiae yeast biomass with increased resistance to osmotic stress and use of biomass
EP22216642.3A EP4273221A1 (en) 2022-05-06 2022-12-23 Method of obtaining saccharomyces cerevisiae yeast biomass with increased resistance to osmotic stress and application of the biomass

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL441114A PL243313B1 (en) 2022-05-06 2022-05-06 Method for obtaining Saccharomyces cerevisiae yeast biomass with increased resistance to osmotic stress and use of biomass

Publications (2)

Publication Number Publication Date
PL441114A1 true PL441114A1 (en) 2022-11-21
PL243313B1 PL243313B1 (en) 2023-07-31

Family

ID=84191852

Family Applications (1)

Application Number Title Priority Date Filing Date
PL441114A PL243313B1 (en) 2022-05-06 2022-05-06 Method for obtaining Saccharomyces cerevisiae yeast biomass with increased resistance to osmotic stress and use of biomass

Country Status (2)

Country Link
EP (1) EP4273221A1 (en)
PL (1) PL243313B1 (en)

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2825715B1 (en) * 2001-06-08 2004-07-16 Lallemand Sa METHOD OF STIMULATION OF DRIED YEASTS ACTIVE FOR ALCOHOLIC FERMENTATION, FERMENTATION METHOD USING THE SAME, AND FERMENTED BEVERAGES OBTAINED
CN1944657A (en) 2006-10-26 2007-04-11 天津科技大学 Method for improving alcohol tolerance of saccharomyces cerevisiae
CN112941119B (en) 2021-01-22 2022-08-30 江南大学 A kind of method for improving the yield of fatty acid ethyl ester of Saccharomyces cerevisiae engineering bacteria

Also Published As

Publication number Publication date
EP4273221A1 (en) 2023-11-08
PL243313B1 (en) 2023-07-31

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