PL441574A1 - Zastosowanie 37-merowej sondy oligonukleotydowej, jako indykatora do monitorowania zmian w metabolizmie komórek nowotworowych - Google Patents

Zastosowanie 37-merowej sondy oligonukleotydowej, jako indykatora do monitorowania zmian w metabolizmie komórek nowotworowych

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Publication number
PL441574A1
PL441574A1 PL441574A PL44157422A PL441574A1 PL 441574 A1 PL441574 A1 PL 441574A1 PL 441574 A PL441574 A PL 441574A PL 44157422 A PL44157422 A PL 44157422A PL 441574 A1 PL441574 A1 PL 441574A1
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PL
Poland
Prior art keywords
metabolism
cancer cells
oligonucleotide probe
indicator
mer oligonucleotide
Prior art date
Application number
PL441574A
Other languages
English (en)
Inventor
Anna Renata Dembska
Natalia Lisiak
Agnieszka Fedoruk-Wyszomirska
Błażej Rubiś
Original Assignee
Uniwersytet Im. Adama Mickiewicza W Poznaniu
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Uniwersytet Im. Adama Mickiewicza W Poznaniu filed Critical Uniwersytet Im. Adama Mickiewicza W Poznaniu
Priority to PL441574A priority Critical patent/PL441574A1/pl
Publication of PL441574A1 publication Critical patent/PL441574A1/pl

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30—Oligonucleotides characterised by their secondary structure
    • C12Q2525/301—Hairpin oligonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/119—Reactions demanding special reaction conditions pH
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2543/00—Reactions characterised by the reaction site, e.g. cell or chromosome
    • C12Q2543/10—Reactions characterised by the reaction site, e.g. cell or chromosome the purpose being "in situ" analysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/10—Detection mode being characterised by the assay principle
    • C12Q2565/101—Interaction between at least two labels
    • C12Q2565/1015—Interaction between at least two labels labels being on the same oligonucleotide

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

Przedmiotem zgłoszenia jest 37-merowa sonda oligonukleotydowa, reagująca na zmiany pH otoczenia, o budowie latarni molekularnej z i-motywem w pętli opartej o sekwencję 5'-GTGATCTAACCC CGC CCC GCC CCG CCC CTACGATCAC-3', znakowanej na końcu 5' donorem i na końcu 3' akceptorem energii do zastosowania jako indykatora o nazwie 488-37RET-590 do monitorowania zmian w metabolizmie komórek nowotworowych, tworzących guzy lite, wywołanych terapeutykami typu antymetabolity i/lub antyhormony.
PL441574A 2022-06-29 2022-06-29 Zastosowanie 37-merowej sondy oligonukleotydowej, jako indykatora do monitorowania zmian w metabolizmie komórek nowotworowych PL441574A1 (pl)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PL441574A PL441574A1 (pl) 2022-06-29 2022-06-29 Zastosowanie 37-merowej sondy oligonukleotydowej, jako indykatora do monitorowania zmian w metabolizmie komórek nowotworowych

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL441574A PL441574A1 (pl) 2022-06-29 2022-06-29 Zastosowanie 37-merowej sondy oligonukleotydowej, jako indykatora do monitorowania zmian w metabolizmie komórek nowotworowych

Publications (1)

Publication Number Publication Date
PL441574A1 true PL441574A1 (pl) 2024-01-03

Family

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Family Applications (1)

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PL441574A PL441574A1 (pl) 2022-06-29 2022-06-29 Zastosowanie 37-merowej sondy oligonukleotydowej, jako indykatora do monitorowania zmian w metabolizmie komórek nowotworowych

Country Status (1)

Country Link
PL (1) PL441574A1 (pl)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100304370A1 (en) * 2009-05-29 2010-12-02 Yamuna Krishnan Intracellular ph sensor using nucleic acid assemblies
US20160002713A1 (en) * 2013-02-26 2016-01-07 National Centre For Biological Sciences (Ncbs-Tifr) Methods of multiplexing dna sensors and localizing dna sensor
CN110331188A (zh) * 2019-07-16 2019-10-15 上海纳米技术及应用国家工程研究中心有限公司 一种细胞内pH的检测方法

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100304370A1 (en) * 2009-05-29 2010-12-02 Yamuna Krishnan Intracellular ph sensor using nucleic acid assemblies
US20160002713A1 (en) * 2013-02-26 2016-01-07 National Centre For Biological Sciences (Ncbs-Tifr) Methods of multiplexing dna sensors and localizing dna sensor
CN110331188A (zh) * 2019-07-16 2019-10-15 上海纳米技术及应用国家工程研究中心有限公司 一种细胞内pH的检测方法

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
A. DEMBSKA ET AL.: "Analytica Chimica Acta, 2017, 990, 157-167", "STUDYING THE INFLUENCE OF STEM COMPOSITION IN PH-SENSITIVE MOLECULAR BEACONS ONTO THEIR SENSING PROPERTIES" *
A. DEMBSKA ET AL.: "Scientific Reports, 2020, 10, 11006", "DEVELOPMENT OF FLUORESCENCE OLIGONUCLEOTIDE PROBES BASED ON CYTOSINE- AND GUANINE-RICH SEQUENCES *

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