PL441645A1 - Method of differentiating benign and malignant thyroid nodule - Google Patents

Method of differentiating benign and malignant thyroid nodule

Info

Publication number
PL441645A1
PL441645A1 PL441645A PL44164522A PL441645A1 PL 441645 A1 PL441645 A1 PL 441645A1 PL 441645 A PL441645 A PL 441645A PL 44164522 A PL44164522 A PL 44164522A PL 441645 A1 PL441645 A1 PL 441645A1
Authority
PL
Poland
Prior art keywords
thyroid nodule
malignant thyroid
differentiating benign
genes
thyroid
Prior art date
Application number
PL441645A
Other languages
Polish (pl)
Inventor
Jolanta Krajewska
Alicja Płuciennik
Małgorzata OCZKO-WOJCIECHOWSKA
Aleksander Płaczek
Michał JARZĄB
Sebastian Student
Agata Wilk
Agnieszka Czarniecka
Aleksandra Pfeifer
Agnieszka Kotecka-Blicharz
Krzysztof Łakomiec
Ewa Chmielik
Dorota Słowińska-Klencka
Marek Dedejcus
Barbara JARZĄB
Krzysztof Fujarewicz
Original Assignee
Narodowy Instytut Onkologii Im. Marii Skłodowskiej-Curie-Państwowy Instytut Badawczy Oddział W Gliwicach
Politechnika Śląska
Polskie Towarzystwo Endokrynologiczne
Uniwersytet Medyczny w Łodzi
Wasko Spółka Akcyjna
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Narodowy Instytut Onkologii Im. Marii Skłodowskiej-Curie-Państwowy Instytut Badawczy Oddział W Gliwicach, Politechnika Śląska, Polskie Towarzystwo Endokrynologiczne, Uniwersytet Medyczny w Łodzi, Wasko Spółka Akcyjna filed Critical Narodowy Instytut Onkologii Im. Marii Skłodowskiej-Curie-Państwowy Instytut Badawczy Oddział W Gliwicach
Priority to PL441645A priority Critical patent/PL441645A1/en
Publication of PL441645A1 publication Critical patent/PL441645A1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/575—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/57557—Immunoassay; Biospecific binding assay; Materials therefor for cancer of other specific parts of the body, e.g. brain
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/112—Disease subtyping, staging or classification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/166—Oligonucleotides used as internal standards, controls or normalisation probes

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Urology & Nephrology (AREA)
  • Microbiology (AREA)
  • Hematology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Oncology (AREA)
  • Hospice & Palliative Care (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Przedmiotem zgłoszenia jest sposób różnicowania łagodnych i złośliwych guzków tarczycy w próbce z biopsji, sklasyfikowanej zgodnie z systemem Bethesda, który obejmuje etapy izolacji RNA oraz określenie poziomu ekspresji znormalizowanego zestawu genów, zawierającego geny takie jak AMOT, DCSTAMP, NOD1, SLC26A7, EMP2, EGR1, MPZL2, ITGA2, MET, SLPI, TPO. Do różnicowania guzków tarczycy wykorzystuje się algorytm klasyfikatora, który nauczony jest na danych ekspresji genów skorelowanych z charakterem guzka tarczycy. Ponadto ustala się prawdopodobieństwo złośliwości badanej próbki.The subject of the application is a method of differentiating benign and malignant thyroid nodules in a biopsy sample, classified according to the Bethesda system, which includes steps of RNA isolation and determination of the expression level of a standardized set of genes, including genes such as AMOT, DCSTAMP, NOD1, SLC26A7, EMP2, EGR1, MPZL2, ITGA2, MET, SLPI, TPO. To differentiate thyroid nodules, a classifier algorithm is used, which is trained on gene expression data correlated with the nature of the thyroid nodule. In addition, the probability of malignancy of the tested sample is determined.

PL441645A 2022-07-05 2022-07-05 Method of differentiating benign and malignant thyroid nodule PL441645A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PL441645A PL441645A1 (en) 2022-07-05 2022-07-05 Method of differentiating benign and malignant thyroid nodule

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL441645A PL441645A1 (en) 2022-07-05 2022-07-05 Method of differentiating benign and malignant thyroid nodule

Publications (1)

Publication Number Publication Date
PL441645A1 true PL441645A1 (en) 2024-01-08

Family

ID=89473649

Family Applications (1)

Application Number Title Priority Date Filing Date
PL441645A PL441645A1 (en) 2022-07-05 2022-07-05 Method of differentiating benign and malignant thyroid nodule

Country Status (1)

Country Link
PL (1) PL441645A1 (en)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2569626A2 (en) * 2010-05-11 2013-03-20 Veracyte, Inc. Methods and compositions for diagnosing conditions
WO2013138726A1 (en) * 2012-03-15 2013-09-19 Sabiosciences Corp. Thyroid cancer biomarker
US20170329894A1 (en) * 2008-11-17 2017-11-16 Veracyte, Inc. Algorithms for disease diagnostics
EP3467123A2 (en) * 2008-11-17 2019-04-10 Veracyte, Inc. Methods and compositions of molecular profiling for disease diagnostics

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20170329894A1 (en) * 2008-11-17 2017-11-16 Veracyte, Inc. Algorithms for disease diagnostics
EP3467123A2 (en) * 2008-11-17 2019-04-10 Veracyte, Inc. Methods and compositions of molecular profiling for disease diagnostics
EP2569626A2 (en) * 2010-05-11 2013-03-20 Veracyte, Inc. Methods and compositions for diagnosing conditions
WO2013138726A1 (en) * 2012-03-15 2013-09-19 Sabiosciences Corp. Thyroid cancer biomarker

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