PL443827A1 - Sposób otrzymywania pęcherzyków zewnątrzkomórkowych (EVs) pochodzących z indukowanych pluripotencjalnych komórek macierzystych (iPSC) oraz ich zastosowanie - Google Patents

Sposób otrzymywania pęcherzyków zewnątrzkomórkowych (EVs) pochodzących z indukowanych pluripotencjalnych komórek macierzystych (iPSC) oraz ich zastosowanie

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Publication number
PL443827A1
PL443827A1 PL443827A PL44382723A PL443827A1 PL 443827 A1 PL443827 A1 PL 443827A1 PL 443827 A PL443827 A PL 443827A PL 44382723 A PL44382723 A PL 44382723A PL 443827 A1 PL443827 A1 PL 443827A1
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Poland
Prior art keywords
stem cells
pluripotent stem
induced pluripotent
extracellular vesicles
derived
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Application number
PL443827A
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English (en)
Inventor
Sylwia BOBIS-WOZOWICZ
Milena PAW
Paweł FERDEK
Marcin PIEJKO
Dawid Wnuk
Agnieszka KUSIAK
Jacek LITEWKA
Kinga STOPA
Original Assignee
Uniwersytet Jagielloński
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Publication date
Application filed by Uniwersytet Jagielloński filed Critical Uniwersytet Jagielloński
Priority to PL443827A priority Critical patent/PL443827A1/pl
Priority to PCT/PL2024/050013 priority patent/WO2024172676A1/en
Priority to EP24718302.3A priority patent/EP4665840A1/en
Publication of PL443827A1 publication Critical patent/PL443827A1/pl

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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
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    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
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    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/28—Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/48—Reproductive organs
    • A61K35/54—Ovaries; Ova; Ovules; Embryos; Foetal cells; Germ cells
    • A61K35/545—Embryonic stem cells; Pluripotent stem cells; Induced pluripotent stem cells; Uncharacterised stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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    • C12N5/005—Protein-free medium
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0607—Non-embryonic pluripotent stem cells, e.g. MASC
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2121/00—Preparations for use in therapy
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    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/45—Artificially induced pluripotent stem cells
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    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins

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  • Engineering & Computer Science (AREA)
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  • Zoology (AREA)
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  • Developmental Biology & Embryology (AREA)
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  • Animal Behavior & Ethology (AREA)
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  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
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  • Reproductive Health (AREA)
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  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Gynecology & Obstetrics (AREA)
  • Transplantation (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

W zgłoszeniu ujawniono lek do leczenia zwłóknień narządowych, który opracowany został na bazie pęcherzyków zewnątrzkomórkowych, pochodzących z indukowanych pluripotencjalnych komórek macierzystych hodowanych w warunkach fizjologicznej hipoksji.
PL443827A 2023-02-18 2023-02-18 Sposób otrzymywania pęcherzyków zewnątrzkomórkowych (EVs) pochodzących z indukowanych pluripotencjalnych komórek macierzystych (iPSC) oraz ich zastosowanie PL443827A1 (pl)

Priority Applications (3)

Application Number Priority Date Filing Date Title
PL443827A PL443827A1 (pl) 2023-02-18 2023-02-18 Sposób otrzymywania pęcherzyków zewnątrzkomórkowych (EVs) pochodzących z indukowanych pluripotencjalnych komórek macierzystych (iPSC) oraz ich zastosowanie
PCT/PL2024/050013 WO2024172676A1 (en) 2023-02-18 2024-02-19 Method for obtaining extracellular vesicles (evs) derived from induced pluripotent stem cells (ipscs) and their use
EP24718302.3A EP4665840A1 (en) 2023-02-18 2024-02-19 Method for obtaining extracellular vesicles (evs) derived from induced pluripotent stem cells (ipscs) and their use

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL443827A PL443827A1 (pl) 2023-02-18 2023-02-18 Sposób otrzymywania pęcherzyków zewnątrzkomórkowych (EVs) pochodzących z indukowanych pluripotencjalnych komórek macierzystych (iPSC) oraz ich zastosowanie

Publications (1)

Publication Number Publication Date
PL443827A1 true PL443827A1 (pl) 2024-08-19

Family

ID=90720481

Family Applications (1)

Application Number Title Priority Date Filing Date
PL443827A PL443827A1 (pl) 2023-02-18 2023-02-18 Sposób otrzymywania pęcherzyków zewnątrzkomórkowych (EVs) pochodzących z indukowanych pluripotencjalnych komórek macierzystych (iPSC) oraz ich zastosowanie

Country Status (3)

Country Link
EP (1) EP4665840A1 (pl)
PL (1) PL443827A1 (pl)
WO (1) WO2024172676A1 (pl)

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20180273906A1 (en) * 2014-04-17 2018-09-27 Muhammad Ashraf Microvesicle and stem cell compositions for therapeutic applications

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
ANDRADE, A.C.; WOLF, M.; BINDER, H.-M.; GOMES, F.G.; MANSTEIN, F.; EBNER-PEKING, P.; POUPARDIN, R.; ZWEIGERDT, R.; SCHALLMOSER, K.: "Int. J. Mol. Sci. 2021, 22, 3890", HYPOXIC CONDITIONS PROMOTE THE ANGIOGENIC POTENTIAL OF HUMAN INDUCED PLURIPOTENT STEM CELL-DERIVED EXTRACELLULAR VESICLES, DOI: https://doi.org/10.3390/ijms22083890 *
BOBIS-WOZOWICZ S, MARBÁN E: "Front. Cell Dev. Biol., 2022, 10:919426", EDITORIAL: EXTRACELLULAR VESICLES AS NEXT GENERATION THERAPEUTICS.,, DOI: 10.3389/fcell.2022.919426 *
GUOKAI CHEN, DANIEL R. GULBRANSON, ZHONGGANG HOU, JENNIFER M. BOLIN, VICTOR RUOTTI, MITCHELL D. PROBASCO, KIMBERLY SMUGA-OTTO, SAR: "Nat Methods.; 2011, 8(5): 424–429", CHEMICALLY DEFINED CONDITIONS FOR HUMAN IPS CELL DERIVATION AND CULTURE, DOI: 10.1038/nmeth.1593 *
LOZANO, J.; RAI, A.; LEES, J.G.; FANG, H.; CLARIDGE, B.; LIM, S.Y.; GREENING, D.W.: "Int. J. Mol. Sci. 2022, 23, 14334", SCALABLE GENERATION OF NANOVESICLES FROM HUMAN-INDUCED PLURIPOTENT STEM CELLS FOR CARDIAC REPAIR, DOI: https://doi.org/10.3390/ijms232214334 *
MARTA ADAMIAK I IN.,: "Circ Res.; 2018, 122(2): 296–309", INDUCED PLURIPOTENT STEM CELL (IPSC)-DERIVED EXTRACELLULAR VESICLES ARE SAFER AND MORE EFFECTIVE FOR CARDIAC REPAIR THAN IPSCS, DOI: 10.1161/CIRCRESAHA.117.311769 *
NEA BISTER, CRISTIANA PISTONO, BENJAMIN HUREMAGIC, JUKKA JOLKKONEN, ROSALBA GIUGNO, TARJA MALM: "J Extracell Vesicles, 2020, 10:e12002", HYPOXIA AND EXTRACELLULAR VESICLES: A REVIEW ON METHODS, VESICULAR CARGO AND FUNCTIONS, DOI: https://doi.org/10.1002/jev2.12002 *

Also Published As

Publication number Publication date
EP4665840A1 (en) 2025-12-24
WO2024172676A1 (en) 2024-08-22

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