PL444576A1 - Soil preparation (biofertilizer) containing live microorganisms capable of binding atmospheric nitrogen, method of manufacturing a soil preparation and microorganisms capable of binding atmospheric nitrogen - Google Patents

Soil preparation (biofertilizer) containing live microorganisms capable of binding atmospheric nitrogen, method of manufacturing a soil preparation and microorganisms capable of binding atmospheric nitrogen

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Publication number
PL444576A1
PL444576A1 PL444576A PL44457623A PL444576A1 PL 444576 A1 PL444576 A1 PL 444576A1 PL 444576 A PL444576 A PL 444576A PL 44457623 A PL44457623 A PL 44457623A PL 444576 A1 PL444576 A1 PL 444576A1
Authority
PL
Poland
Prior art keywords
soil preparation
microorganisms capable
biomass
binding
biofertilizer
Prior art date
Application number
PL444576A
Other languages
Polish (pl)
Inventor
Roman Marecik
Katarzyna CZACZYK
Wojciech Białas
Anna Sip
Agnieszka Wita
Michał Słodziński
Anna Dobrowolska
Katarzyna Zarobkiewicz
Original Assignee
Uniwersytet Przyrodniczy W Poznaniu
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Uniwersytet Przyrodniczy W Poznaniu filed Critical Uniwersytet Przyrodniczy W Poznaniu
Priority to PL444576A priority Critical patent/PL444576A1/en
Publication of PL444576A1 publication Critical patent/PL444576A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00—Other organic fertilisers
    • C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N2300/00—Combinations or mixtures of active ingredients covered by classes A01N27/00 - A01N65/48 with other active or formulation relevant ingredients, e.g. specific carrier materials or surfactants, covered by classes A01N25/00 - A01N65/48
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/065—Azotobacter

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Virology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Pest Control & Pesticides (AREA)
  • Environmental Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Dentistry (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biomedical Technology (AREA)
  • Agronomy & Crop Science (AREA)
  • General Engineering & Computer Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Soil Conditioners And Soil-Stabilizing Materials (AREA)

Abstract

Przedmiotem zgłoszenia jest preparat doglebowy (bionawóz) zawierający żywe mikroorganizmy zdolne do wiązania atmosferycznego jaki ma postać liofilizatu nowego szczepu Azotobacter chroococcum KBIMZ6. Zgłoszenie zawiera także sposób wytwarzania preparatu doglebowego (bionawozu), zawierającego żywe mikroorganizmy zdolne do wiązania atmosferycznego, w którym, w kolejnych krokach: 1. Z podłoża z uprawy soi izoluje się bakterie z rodzaju Azotobacter, prowadzi się hodowlę wgłębną, korzystnie kontroluje się zdolność uzyskanych mikroorganizmów do wiązania azotu atmosferycznego i pozyskuje biomasę komórkową; 2. Z pozyskanej biomasy izoluje się DNA, identyfikuje się poprzez analizę porównawczą sekwencji genów 16S rRNA izolat KBIMZ 6; 3. Z uzyskanego izolatu KBIMZ 6 hoduje się biomasę w hodowlach okresowych; 4. Równolegle z uzyskanego izolatu KBIMZ 6 hoduje się biomasę w hodowlach okresowych z zasilaniem; 5. Liofilizuje się biomasę z płynu pohodowlanego uzyskanego w hodowlach okresowych lub hodowlach okresowych z zasilaniem.The subject of the application is a soil preparation (biofertilizer) containing living microorganisms capable of atmospheric binding in the form of a lyophilisate of a new strain of Azotobacter chroococcum KBIMZ6. The application also includes a method for producing a soil preparation (biofertilizer) containing living microorganisms capable of atmospheric binding, in which, in the following steps: 1. Bacteria of the genus Azotobacter are isolated from a soybean cultivation substrate, a submerged culture is carried out, the ability of the obtained microorganisms to bind atmospheric nitrogen is preferably controlled, and the cellular biomass is obtained; 2. DNA is isolated from the obtained biomass, the KBIMZ 6 isolate is identified by comparative analysis of the 16S rRNA gene sequences; 3. The biomass is grown from the obtained KBIMZ 6 isolate in batch cultures; 4. In parallel, biomass is cultivated from the obtained KBIMZ 6 isolate in fed-batch cultures; 5. Biomass is freeze-dried from the post-culture fluid obtained in batch cultures or fed-batch cultures.

PL444576A 2023-04-25 2023-04-25 Soil preparation (biofertilizer) containing live microorganisms capable of binding atmospheric nitrogen, method of manufacturing a soil preparation and microorganisms capable of binding atmospheric nitrogen PL444576A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PL444576A PL444576A1 (en) 2023-04-25 2023-04-25 Soil preparation (biofertilizer) containing live microorganisms capable of binding atmospheric nitrogen, method of manufacturing a soil preparation and microorganisms capable of binding atmospheric nitrogen

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL444576A PL444576A1 (en) 2023-04-25 2023-04-25 Soil preparation (biofertilizer) containing live microorganisms capable of binding atmospheric nitrogen, method of manufacturing a soil preparation and microorganisms capable of binding atmospheric nitrogen

Publications (1)

Publication Number Publication Date
PL444576A1 true PL444576A1 (en) 2024-10-28

Family

ID=93289640

Family Applications (1)

Application Number Title Priority Date Filing Date
PL444576A PL444576A1 (en) 2023-04-25 2023-04-25 Soil preparation (biofertilizer) containing live microorganisms capable of binding atmospheric nitrogen, method of manufacturing a soil preparation and microorganisms capable of binding atmospheric nitrogen

Country Status (1)

Country Link
PL (1) PL444576A1 (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2193085A1 (en) * 1972-07-19 1974-02-15 Sera Soil improving agent - contg particular strains of soil bacteria
CN104774772A (en) * 2015-04-16 2015-07-15 乐山师范学院 Straw decomposing fungicide with fertility increasing effect and application of fungicide

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2193085A1 (en) * 1972-07-19 1974-02-15 Sera Soil improving agent - contg particular strains of soil bacteria
CN104774772A (en) * 2015-04-16 2015-07-15 乐山师范学院 Straw decomposing fungicide with fertility increasing effect and application of fungicide

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
MICHAEL JAMES VAN OOSTEN I IN.,: "BMC Plant Biol 18, 205 (2018).", ROOT INOCULATION WITH AZOTOBACTER CHROOCOCCUM 76A ENHANCES TOMATO PLANTS ADAPTATION TO SALT STRESS UNDER LOW N CONDITIONS, DOI: https://doi.org/10.1186/s12870-018-1411-5 *
MONIKA KOZIEŁ, ANNA GAŁĄZKA, STEFAN MARTYNIUK: "Studia i Raporty IUNG-PIB, Zeszyt 56(10): 57-70, 2018,", WOLNOŻYJĄCE BAKTERIE WIĄŻĄCE AZOT ATMOSFERYCZNY Z RODZAJU AZOTOBACTER – WYSTĘPOWANIE, LICZEBNOŚĆ I ZNACZENIE, DOI: 10.26114/sir.iung.2018.56.05 *
SATISH KUMAR, DIKSHA, SATYAVIR S. SINDHU, RAKESH KUMAR: "Current Research in Microbial Sciences, Volume 3, 2022, 100094", BIOFERTILIZERS: AN ECOFRIENDLY TECHNOLOGY FOR NUTRIENT RECYCLING AND ENVIRONMENTAL SUSTAINABILITY, DOI: doi.org/10.1016/j.crmicr.2021.100094 *

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