PL445985A1 - Method for obtaining extracts of algal origin with a high concentration of antioxidants and use of extracts from green algae Klebsormidium dissectum in food, pharmaceutical and cosmetic products - Google Patents

Method for obtaining extracts of algal origin with a high concentration of antioxidants and use of extracts from green algae Klebsormidium dissectum in food, pharmaceutical and cosmetic products

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Publication number
PL445985A1
PL445985A1 PL445985A PL44598523A PL445985A1 PL 445985 A1 PL445985 A1 PL 445985A1 PL 445985 A PL445985 A PL 445985A PL 44598523 A PL44598523 A PL 44598523A PL 445985 A1 PL445985 A1 PL 445985A1
Authority
PL
Poland
Prior art keywords
extracts
green algae
klebsormidium
dissectum
antioxidants
Prior art date
Application number
PL445985A
Other languages
Polish (pl)
Other versions
PL249509B1 (en
Inventor
Michał Adamski
Original Assignee
Instytut Botaniki Im. Władysława Szafera Polskiej Akademii Nauk
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Instytut Botaniki Im. Władysława Szafera Polskiej Akademii Nauk filed Critical Instytut Botaniki Im. Władysława Szafera Polskiej Akademii Nauk
Priority to PL445985A priority Critical patent/PL249509B1/en
Publication of PL445985A1 publication Critical patent/PL445985A1/en
Publication of PL249509B1 publication Critical patent/PL249509B1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C12P1/04—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using bacteria
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/06—Fungi, e.g. yeasts
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9706—Algae
    • A61K8/9722—Chlorophycota or Chlorophyta [green algae], e.g. Chlorella
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D11/00—Solvent extraction
    • B01D11/02—Solvent extraction of solids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D11/00—Solvent extraction
    • B01D11/02—Solvent extraction of solids
    • B01D11/0261—Solvent extraction of solids comprising vibrating mechanisms, e.g. mechanical, acoustical
    • B01D11/0265—Applying ultrasound

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Botany (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Animal Behavior & Ethology (AREA)
  • Zoology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Epidemiology (AREA)
  • Acoustics & Sound (AREA)
  • Mechanical Engineering (AREA)
  • Birds (AREA)
  • Medicinal Chemistry (AREA)
  • Medical Informatics (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Nutrition Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Cosmetics (AREA)
  • Anti-Oxidant Or Stabilizer Compositions (AREA)

Abstract

Przedmiotem zgłoszenia jest sposób uzyskania ekstraktów pochodzenia glonowego o wysokim stężeniu antyoksydantów charakteryzujący się tym, że kultywowane w pożywce płynnej komórki glonów Klebsormidium dissectum (zielenicy) oddziela się od pożywki poprzez filtrację próżniową, a następnie filtry umieszcza się w zamrażarce i przetrzymuje w temperaturze -20°C w czasie nie krótszym niż 1 h, po czym wyjmuje z zamrażarki i poddaje działaniu temperatury z zakresu od 19°C do 22°C w czasie nie krótszym niż 1 h i proces ten powtarza jeszcze dwukrotnie do uzyskania zniszczenia błon komórkowych i naruszenia ścian komórkowych glonów, a następnie uzyskany materiał liofilizuje się, po czym łączy z rozpuszczalnikiem w proporcji 1:3 w/w, sonifikuje ultradźwiękami i wytrząsa do całkowitego odbarwienia pozostałości filtrów; uzyskane roztwory wiruje się różnicowo 10 000 x g do oddzielenia frakcji supernatantu od frakcji pelletu, a otrzymany supernatant filtruje się i do momentu wykorzystania przetrzymuje w zamrażarce w temperaturze -20°C. Przedmiotem zgłoszenia jest także zastosowanie ekstraktów z zielenicy w produktach żywnościowych, farmaceutycznych i kosmetycznych.The subject of the application is a method for obtaining extracts of algal origin with a high concentration of antioxidants, characterized by the fact that Klebsormidium dissectum (green algae) algae cells cultivated in a liquid medium are separated from the medium by vacuum filtration, and then the filters are placed in a freezer and kept at a temperature of -20° C for no less than 1 hour, then removed from the freezer and exposed to temperatures ranging from 19°C to 22°C for no less than 1 hour, and repeated this process twice more until the cell membranes were destroyed and the algae cell walls were damaged. , and then the obtained material is freeze-dried, then combined with the solvent in a 1:3 w/w ratio, sonicated with ultrasound and shaken until the filter residues are completely discolored; the obtained solutions are differentially centrifuged 10,000 x g to separate the supernatant fraction from the pellet fraction, and the obtained supernatant is filtered and kept in a freezer at -20°C until use. The subject of the application is also the use of green algae extracts in food, pharmaceutical and cosmetic products.

PL445985A 2023-09-01 2023-09-01 Method for obtaining extracts of algal origin with a high concentration of antioxidants and use of extracts from green algae Klebsormidium dissectum in food, pharmaceutical and cosmetic products PL249509B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PL445985A PL249509B1 (en) 2023-09-01 2023-09-01 Method for obtaining extracts of algal origin with a high concentration of antioxidants and use of extracts from green algae Klebsormidium dissectum in food, pharmaceutical and cosmetic products

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL445985A PL249509B1 (en) 2023-09-01 2023-09-01 Method for obtaining extracts of algal origin with a high concentration of antioxidants and use of extracts from green algae Klebsormidium dissectum in food, pharmaceutical and cosmetic products

Publications (2)

Publication Number Publication Date
PL445985A1 true PL445985A1 (en) 2024-02-12
PL249509B1 PL249509B1 (en) 2026-05-04

Family

ID=89855700

Family Applications (1)

Application Number Title Priority Date Filing Date
PL445985A PL249509B1 (en) 2023-09-01 2023-09-01 Method for obtaining extracts of algal origin with a high concentration of antioxidants and use of extracts from green algae Klebsormidium dissectum in food, pharmaceutical and cosmetic products

Country Status (1)

Country Link
PL (1) PL249509B1 (en)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006028412A (en) * 2004-07-20 2006-02-02 Kansai Electric Power Co Inc:The Antioxidant derived from green algae
EP1806411A1 (en) * 2006-01-05 2007-07-11 Cognis IP Management GmbH Process for obtaining zeaxanthin from algae
EP2026759A2 (en) * 2006-05-05 2009-02-25 Blaa Lonid Hf. Pharmaceutical and cosmetic use of extracts from algae obtainable from saline hot water sources
US20200232003A1 (en) * 2019-01-16 2020-07-23 Kuehnle Agrosystems, Inc. Subterranean microalgae for production of microbial biomass, substances, and compositions

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006028412A (en) * 2004-07-20 2006-02-02 Kansai Electric Power Co Inc:The Antioxidant derived from green algae
EP1806411A1 (en) * 2006-01-05 2007-07-11 Cognis IP Management GmbH Process for obtaining zeaxanthin from algae
EP2026759A2 (en) * 2006-05-05 2009-02-25 Blaa Lonid Hf. Pharmaceutical and cosmetic use of extracts from algae obtainable from saline hot water sources
US20200232003A1 (en) * 2019-01-16 2020-07-23 Kuehnle Agrosystems, Inc. Subterranean microalgae for production of microbial biomass, substances, and compositions

Also Published As

Publication number Publication date
PL249509B1 (en) 2026-05-04

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