PL450069A1 - Pair of oligonucleotide primers for detection and method for detecting the recessive allele of the crown rust resistance gene Pc101 derived from Avena sterilis PI 334961 in the genome of common oat (Avena sativa L.) - Google Patents

Pair of oligonucleotide primers for detection and method for detecting the recessive allele of the crown rust resistance gene Pc101 derived from Avena sterilis PI 334961 in the genome of common oat (Avena sativa L.)

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Publication number
PL450069A1
PL450069A1 PL450069A PL45006924A PL450069A1 PL 450069 A1 PL450069 A1 PL 450069A1 PL 450069 A PL450069 A PL 450069A PL 45006924 A PL45006924 A PL 45006924A PL 450069 A1 PL450069 A1 PL 450069A1
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Poland
Prior art keywords
pair
avena
resistance gene
rust resistance
recessive allele
Prior art date
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PL450069A
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Polish (pl)
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PL248753B1 (en
Inventor
Edyta Paczos-Grzęda
Joanna Lech
Sylwia Sowa
Aneta Koroluk
Krzysztof Kowalczyk
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Uniwersytet Przyrodniczy W Lublinie
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Application filed by Uniwersytet Przyrodniczy W Lublinie filed Critical Uniwersytet Przyrodniczy W Lublinie
Priority to PL450069A priority Critical patent/PL248753B1/en
Publication of PL450069A1 publication Critical patent/PL450069A1/en
Publication of PL248753B1 publication Critical patent/PL248753B1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Botany (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Przedmiot zgłoszenia stanowi para oligonukleotydowych starterów do wykrywania allelu recesywnego genu odporności na rdzę koronową Pc101 z Avena sterilis PI 334961 w roślinach owsa o sekwencjach nr 1 i 2 przedstawionych na liście sekwencji. Przedmiotem zgłoszenia jest ponadto sposób identyfikacji allelu recesywnego genu odporności na rdzę koronowa Pc101 z Avena sterilis PI 334961 w roślinach owsa, w którym polimorficzny fragment DNA sprzężony z badanym genem amplifikowany jest w reakcji PCR z zastosowaniem pary starterów, po czym dokonuje się detekcji produktu amplifikacji, a parę starterów stanowi para oligonukleotydów o sekwencjach nr 1 i 2 przedstawionych na liście sekwencji, przy czym stosuje się marker 293_T. W wyniku PCR amplifikowany jest fragment DNA o długości 139 par zasad o sekwencji nr 3 przedstawionej na liście sekwencji.The subject of the application is a pair of oligonucleotide primers for detecting the recessive allele of the crown rust resistance gene Pc101 from Avena sterilis PI 334961 in oat plants with sequences no. 1 and 2 presented in the sequence listing. The subject of the application is also a method for identifying the recessive allele of the crown rust resistance gene Pc101 from Avena sterilis PI 334961 in oat plants, wherein a polymorphic DNA fragment coupled to the gene being tested is amplified in a PCR reaction using a pair of primers, followed by detection of the amplification product, and the pair of primers is a pair of oligonucleotides with sequences no. 1 and 2 presented in the sequence listing, wherein the 293_T marker is used. As a result of the PCR, a 139-base-pair DNA fragment is amplified with sequence no. 3 presented in the sequence listing.

PL450069A 2024-10-17 2024-10-17 A pair of oligonucleotide primers for detection and a method for detecting the recessive allele of the crown rust resistance gene Pc101 derived from Avena sterilis PI 334961 in the genome of common oat (Avena sativa L.) PL248753B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PL450069A PL248753B1 (en) 2024-10-17 2024-10-17 A pair of oligonucleotide primers for detection and a method for detecting the recessive allele of the crown rust resistance gene Pc101 derived from Avena sterilis PI 334961 in the genome of common oat (Avena sativa L.)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL450069A PL248753B1 (en) 2024-10-17 2024-10-17 A pair of oligonucleotide primers for detection and a method for detecting the recessive allele of the crown rust resistance gene Pc101 derived from Avena sterilis PI 334961 in the genome of common oat (Avena sativa L.)

Publications (2)

Publication Number Publication Date
PL450069A1 true PL450069A1 (en) 2025-08-04
PL248753B1 PL248753B1 (en) 2026-01-26

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Application Number Title Priority Date Filing Date
PL450069A PL248753B1 (en) 2024-10-17 2024-10-17 A pair of oligonucleotide primers for detection and a method for detecting the recessive allele of the crown rust resistance gene Pc101 derived from Avena sterilis PI 334961 in the genome of common oat (Avena sativa L.)

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PL (1) PL248753B1 (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL422536A1 (en) * 2017-08-11 2019-02-25 Uniwersytet Przyrodniczy W Lublinie A pair of starter oligonucleotides for detecting, and the method for detecting of the recessive allele of immunity gene Pc39 to crown rust in the plants of common oat (Avena sativa L.)
PL438756A1 (en) * 2021-08-16 2023-02-20 Uniwersytet Przyrodniczy W Lublinie Pair of oligonucleotide primers for detection and method for detecting the allele of the recessive crown rust resistance gene from the subline of the hybrid form Avena sativa 'Pendek' x Avena sterilis CW-486 in oat plants (Avena sativa L.)
PL440934A1 (en) * 2022-04-13 2023-10-16 Uniwersytet Przyrodniczy W Lublinie Pair of oligonucleotide primers for the detection and method of detecting the allele of the dominant crown rust resistance gene from the subline of the hybrid form A. sterilis PI 296244 x A. sativa TAM-O-312 in oat plants (Avena sativa L.)

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL422536A1 (en) * 2017-08-11 2019-02-25 Uniwersytet Przyrodniczy W Lublinie A pair of starter oligonucleotides for detecting, and the method for detecting of the recessive allele of immunity gene Pc39 to crown rust in the plants of common oat (Avena sativa L.)
PL438756A1 (en) * 2021-08-16 2023-02-20 Uniwersytet Przyrodniczy W Lublinie Pair of oligonucleotide primers for detection and method for detecting the allele of the recessive crown rust resistance gene from the subline of the hybrid form Avena sativa 'Pendek' x Avena sterilis CW-486 in oat plants (Avena sativa L.)
PL440934A1 (en) * 2022-04-13 2023-10-16 Uniwersytet Przyrodniczy W Lublinie Pair of oligonucleotide primers for the detection and method of detecting the allele of the dominant crown rust resistance gene from the subline of the hybrid form A. sterilis PI 296244 x A. sativa TAM-O-312 in oat plants (Avena sativa L.)

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
PARK RF AT AL.: "Theor Appl Genet. 2022 Nov;135(11):3709-3734. doi: 10.1007/s00122-022-04121-z. Epub 2022 Jun 4", BREEDING OAT FOR RESISTANCE TO THE CROWN RUST PATHOGEN PUCCINIA CORONATA F. SP. AVENAE: ACHIEVEMENTS AND PROSPECTS *

Also Published As

Publication number Publication date
PL248753B1 (en) 2026-01-26

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