PT101209A - Isolated cDNA sequences corresp. to self-incompatability alleles in Brassica campestris and B napus ssp. rapifera - are useful for transferring self-incompatibility phenotype to plant cells and protoplasts - Google Patents
Isolated cDNA sequences corresp. to self-incompatability alleles in Brassica campestris and B napus ssp. rapifera - are useful for transferring self-incompatibility phenotype to plant cells and protoplastsInfo
- Publication number
- PT101209A PT101209A PT101209A PT10120993A PT101209A PT 101209 A PT101209 A PT 101209A PT 101209 A PT101209 A PT 101209A PT 10120993 A PT10120993 A PT 10120993A PT 101209 A PT101209 A PT 101209A
- Authority
- PT
- Portugal
- Prior art keywords
- self
- rapifera
- brassica
- plant cells
- protoplasts
- Prior art date
Links
- 108700028369 Alleles Proteins 0.000 title abstract 4
- 241000196324 Embryophyta Species 0.000 title abstract 4
- 235000005637 Brassica campestris Nutrition 0.000 title abstract 2
- 241001301148 Brassica rapa subsp. oleifera Species 0.000 title abstract 2
- 210000004027 cell Anatomy 0.000 title abstract 2
- 239000002299 complementary DNA Substances 0.000 title abstract 2
- 210000001938 protoplast Anatomy 0.000 title abstract 2
- 230000005849 recognition of pollen Effects 0.000 title abstract 2
- 241000219198 Brassica Species 0.000 abstract 3
- 235000011331 Brassica Nutrition 0.000 abstract 3
- 108090000623 proteins and genes Proteins 0.000 abstract 3
- 108091034117 Oligonucleotide Proteins 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 241000589158 Agrobacterium Species 0.000 abstract 1
- 240000002791 Brassica napus Species 0.000 abstract 1
- 235000011293 Brassica napus Nutrition 0.000 abstract 1
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 238000001514 detection method Methods 0.000 abstract 1
- 239000013598 vector Substances 0.000 abstract 1
Landscapes
- Motor Or Generator Current Collectors (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
Isolated cDNA's of the self-incompatibility (S-) loci of (1) Brassica napus ssp. rapifera and (2) Brassica campestris are claimed comprising sequences as defined in the specification. Also claimed are: (i) an oligonucleotide with homology to s-locus genes of Brassica consisting of sequence CTTGTGGCAAAGTTTCGATT (SEQ. ID. No.3); (ii) an oligonucleotide with homology to S-locus genes of Brassica consisting of sequence CTGACATAAAGATCTTG (SEQ. ID. No.4); (iii) a method of identifying and amplifying DNA sequences with homology to S-locus genes; and (iv) methods for detection of the S-locus allele, the 910 allele and the A14 allele in a Brassica seedling. Pref. the bacterium Agrobacterium tumifaciens is pref. used to introduce the vectors into SC plants, plant cells and plant protoplasts.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US84756492A | 1992-03-03 | 1992-03-03 | |
| PCT/US1992/004530 WO1993018149A1 (en) | 1992-03-03 | 1992-06-29 | Self-incompatibility alleles of brassica |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| PT101209A true PT101209A (en) | 1994-03-31 |
Family
ID=26784807
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PT101209A PT101209A (en) | 1992-03-03 | 1993-03-03 | Isolated cDNA sequences corresp. to self-incompatability alleles in Brassica campestris and B napus ssp. rapifera - are useful for transferring self-incompatibility phenotype to plant cells and protoplasts |
Country Status (2)
| Country | Link |
|---|---|
| AU (1) | AU2336392A (en) |
| PT (1) | PT101209A (en) |
-
1992
- 1992-06-29 AU AU23363/92A patent/AU2336392A/en not_active Abandoned
-
1993
- 1993-03-03 PT PT101209A patent/PT101209A/en not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| AU2336392A (en) | 1993-10-05 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| BB1A | Laying open of patent application |
Effective date: 19930820 |
|
| FC3A | Refusal |
Effective date: 19990702 |