PT1931998E - Método de quantificação de alergénios - Google Patents

Método de quantificação de alergénios Download PDF

Info

Publication number: PT1931998E
Authority: PT; Portugal
Prior art keywords: allergen; der; peptides; allergens; sample
Prior art date: 2005-09-15

Application number

PT06775965T

Other languages

English (en)

Portuguese (pt)

Inventor

Ulla Seppaelae

Original Assignee

Alk Abello As

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2005-09-15

Filing date

2006-09-01

Publication date

2010-09-13

2006-09-01 Application filed by Alk Abello As filed Critical Alk Abello As

2010-09-13 Publication of PT1931998E publication Critical patent/PT1931998E/pt

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230000000155 isotopic effect Effects 0.000 description 1
238000001948 isotopic labelling Methods 0.000 description 1
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125000003588 lysine group Chemical group [H]N([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])(N([H])[H])C(*)=O 0.000 description 1
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238000002703 mutagenesis Methods 0.000 description 1
231100000350 mutagenesis Toxicity 0.000 description 1
239000012038 nucleophile Substances 0.000 description 1
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125000002347 octyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
150000002894 organic compounds Chemical class 0.000 description 1
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125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 description 1
238000011197 physicochemical method Methods 0.000 description 1
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230000004481 post-translational protein modification Effects 0.000 description 1
239000000843 powder Substances 0.000 description 1
239000002244 precipitate Substances 0.000 description 1
238000001556 precipitation Methods 0.000 description 1
150000003141 primary amines Chemical class 0.000 description 1
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230000013777 protein digestion Effects 0.000 description 1
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230000002797 proteolythic effect Effects 0.000 description 1
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210000001519 tissue Anatomy 0.000 description 1
QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
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Classifications

- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6818—Sequencing of polypeptides
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2458/00—Labels used in chemical analysis of biological material
- G01N2458/15—Non-radioactive isotope labels, e.g. for detection by mass spectrometry

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Life Sciences & Earth Sciences (AREA)
Health & Medical Sciences (AREA)
Engineering & Computer Science (AREA)
Molecular Biology (AREA)
Hematology (AREA)
Chemical & Material Sciences (AREA)
Urology & Nephrology (AREA)
Physics & Mathematics (AREA)
Immunology (AREA)
Biomedical Technology (AREA)
Biophysics (AREA)
Food Science & Technology (AREA)
Biotechnology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Microbiology (AREA)
Bioinformatics & Computational Biology (AREA)
Bioinformatics & Cheminformatics (AREA)
Cell Biology (AREA)
Medicinal Chemistry (AREA)
Analytical Chemistry (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
General Physics & Mathematics (AREA)
Pathology (AREA)
Peptides Or Proteins (AREA)
Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

PT06775965T 2005-09-15 2006-09-01 Método de quantificação de alergénios PT1931998E (pt)

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DK (2)	DK1931998T3 (da)
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PT (1)	PT1931998E (da)

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RU2013119982A (ru) *	2010-10-01	2014-11-20	ДАУ АГРОСАЙЕНСИЗ ЭлЭлСи	Количественное определение и характеризация аллергенов
US8952324B2 (en) *	2011-01-07	2015-02-10	Hitachi High-Technologies Corporation	Mass analyzing apparatus, analyzing method and calibration sample
CN106918709B (zh) *	2011-09-02	2020-03-10	Dh科技发展私人贸易有限公司	检测过敏原的系统和方法
CA2958074A1 (en) *	2014-08-11	2016-02-18	Dow Agrosciences Llc	Systems and methods for selective quantitation and detection of allergens
BR112017002622A2 (pt) *	2014-08-11	2018-02-20	Dow Agrosciences Llc	métodos e sistemas para quantificação seletiva e detecção de alergênicos
CN106033087B (zh) *	2015-03-18	2018-05-18	王峥	内置性标准曲线检测物质分子数之方法系统
CN107525934A (zh) *	2016-06-21	2017-12-29	张效龙	一种测定血液中抗体氨基酸序列的方法
CN107064338B (zh) *	2017-03-01	2019-12-27	国家烟草质量监督检验中心	一种基于iTRAQ标记测定烟碱诱导细胞差异表达蛋白质的方法
CN114354798B (zh) *	2021-12-30	2024-12-24	广电计量检测(无锡)有限公司	一种基于二维色谱分离测定肽类产品中游离氨基酸的方法及其应用
CN117783539A (zh) *	2023-12-26	2024-03-29	中国医学科学院北京协和医院	一种花粉变应原制剂中致敏蛋白的定量检测方法

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- 2006-09-01 ES ES06775965T patent/ES2347277T3/es active Active
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ES2347277T3 (es)	2010-10-27
CN101287991B (zh)	2013-05-08
DK2228656T3 (da)	2014-02-03
DK1931998T3 (da)	2010-10-11
CN101287991A (zh)	2008-10-15

Publication	Publication Date	Title
US10023900B2 (en)	2018-07-17	Method for quantification of allergens
Kirsch et al.	2009	Quantitative methods for food allergens: a review
CN101600959B (zh)	2013-10-30	肽和蛋白的定量方法
US7445907B2 (en)	2008-11-04	Methods for mass spectrometry detection and quantification of specific target proteins in complex biological samples
JP2010509567A (ja)	2010-03-25	質量分光測定法の分析において多重化することを可能にするためのポリペプチドの二重のラベリングのための化合物及び方法
PT1931998E (pt)	2010-09-13	Método de quantificação de alergénios
US20180011107A1 (en)	2018-01-11	Identification of the presence of specific polypeptides by liquid chromatography and mass spectrometry
US6969757B2 (en)	2005-11-29	Differential labeling for quantitative analysis of complex protein mixtures
EP1704507B1 (en)	2009-09-16	Methods and system for the identification and charcterization of peptides and their functional relationships by use of measures of correlation
JP6130448B2 (ja)	2017-05-17	草種の同定のための方法
Bayard et al.	2001	Bioanalytical characterization of proteins
HK1120303B (en)	2010-11-19	A method for quantification of allergens
HK1148583B (en)	2014-04-25	Quantification of allergens
TWI351518B (da)	2011-11-01
Angel	2007	Quantitative proteomics: methodologies and comparative analysis of differentiating murine embryonic stem cells
Cavatorta	2008	Peptides and proteins as markers of quality and safety in food: an approach by Mass Spectrometry
Darville et al.	2013	Mass spectrometry-based proteomics for structure and function determination of alligator leukocyte proteins