PT88975A - Processo para o isolamento e purificacao de hirudina - Google Patents

Processo para o isolamento e purificacao de hirudina

Info

Publication number: PT88975A
Authority: PT; Portugal
Prior art keywords: hydrudine; purification; insulation; hirudin; purified
Prior art date: 1987-11-13

Application number

PT88975A

Other languages

English (en)

Other versions

PT88975B (pt

Inventor

Paul Habermann

Dominique Tripier

Peter Crausse

Werner Badziong

Original Assignee

Hoechst Ag

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1987-11-13

Filing date

1988-11-10

Publication date

1989-11-30

1988-11-10 Application filed by Hoechst Ag filed Critical Hoechst Ag

1989-11-30 Publication of PT88975A publication Critical patent/PT88975A/pt

1994-11-30 Publication of PT88975B publication Critical patent/PT88975B/pt

Links

238000009413 insulation Methods 0.000 title 1
238000000034 method Methods 0.000 title 1
238000000746 purification Methods 0.000 title 1
230000005526 G1 to G0 transition Effects 0.000 abstract 1
102000007625 Hirudins Human genes 0.000 abstract 1
108010007267 Hirudins Proteins 0.000 abstract 1
238000004587 chromatography analysis Methods 0.000 abstract 1
WQPDUTSPKFMPDP-OUMQNGNKSA-N hirudin Chemical compound C([C@@H](C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC=1C=CC(OS(O)(=O)=O)=CC=1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CCCCN)NC(=O)[C@H]1N(CCC1)C(=O)[C@@H](NC(=O)CNC(=O)[C@H](CCC(O)=O)NC(=O)CNC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@H]1NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCC(O)=O)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CO)NC(=O)CNC(=O)[C@H](CC(C)C)NC(=O)[C@H]([C@@H](C)CC)NC(=O)[C@@H]2CSSC[C@@H](C(=O)N[C@@H](CCC(O)=O)C(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@H](C(=O)N[C@H](C(NCC(=O)N[C@@H](CCC(N)=O)C(=O)NCC(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCCCN)C(=O)N2)=O)CSSC1)C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H]1NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)CNC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=2C=CC(O)=CC=2)NC(=O)[C@@H](NC(=O)[C@@H](N)C(C)C)C(C)C)[C@@H](C)O)CSSC1)C(C)C)[C@@H](C)O)[C@@H](C)O)C1=CC=CC=C1 WQPDUTSPKFMPDP-OUMQNGNKSA-N 0.000 abstract 1
229940006607 hirudin Drugs 0.000 abstract 1
230000002209 hydrophobic effect Effects 0.000 abstract 1
239000003960 organic solvent Substances 0.000 abstract 1
229920005989 resin Polymers 0.000 abstract 1
239000011347 resin Substances 0.000 abstract 1
150000003839 salts Chemical class 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
- C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin

Landscapes

Chemical & Material Sciences (AREA)
Health & Medical Sciences (AREA)
Organic Chemistry (AREA)
Biophysics (AREA)
Life Sciences & Earth Sciences (AREA)
Biochemistry (AREA)
Gastroenterology & Hepatology (AREA)
General Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Medicinal Chemistry (AREA)
Molecular Biology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Tropical Medicine & Parasitology (AREA)
Peptides Or Proteins (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Treatment Of Liquids With Adsorbents In General (AREA)
Extrusion Moulding Of Plastics Or The Like (AREA)
Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
Magnetic Heads (AREA)
Professional, Industrial, Or Sporting Protective Garments (AREA)
Insulated Conductors (AREA)
Orthopedics, Nursing, And Contraception (AREA)
Cable Accessories (AREA)
Graft Or Block Polymers (AREA)
Solid-Sorbent Or Filter-Aiding Compositions (AREA)
Seal Device For Vehicle (AREA)
Communication Cables (AREA)
Two-Way Televisions, Distribution Of Moving Picture Or The Like (AREA)

PT88975A 1987-11-13 1988-11-10 Processo para o isolamento e purificacao de hirudina PT88975B (pt)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
DE19873738541 DE3738541A1 (de)	1987-11-13	1987-11-13	Verfahren zur isolierung und reinigung von hirudin

Publications (2)

Publication Number	Publication Date
PT88975A true PT88975A (pt)	1989-11-30
PT88975B PT88975B (pt)	1994-11-30

Family

ID=6340409

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
PT88975A PT88975B (pt)	1987-11-13	1988-11-10	Processo para o isolamento e purificacao de hirudina

Country Status (9)

Country	Link
US (1)	US5095092A (pt)
EP (1)	EP0316650B1 (pt)
JP (1)	JP2756279B2 (pt)
AT (1)	ATE100109T1 (pt)
DE (2)	DE3738541A1 (pt)
DK (1)	DK173366B1 (pt)
ES (1)	ES2049238T3 (pt)
IE (1)	IE62538B1 (pt)
PT (1)	PT88975B (pt)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5268296A (en) *	1988-06-11	1993-12-07	Ciba-Geigy Corporation	DNA vector and recombinant host cell for production of hirullin P6 and P18
DE3835815A1 (de) *	1988-10-21	1990-04-26	Hoechst Ag	Neue isohirudine
DE4140381A1 (de) *	1991-12-07	1993-06-09	Hoechst Ag, 6230 Frankfurt, De	Neue synthetische isohirudine mit verbesserter stabilitaet
DE4404168A1 (de) *	1994-02-10	1995-08-17	Hoechst Ag	Hirudinderivate und Verfahren zu deren Herstellung
KR0141651B1 (ko) *	1994-09-07	1998-06-15	강재헌	금속이온 친화성 크로마토그라피를 이용한 히루딘의 정제방법
DE10108211A1 (de) *	2001-02-20	2002-08-22	Aventis Pharma Gmbh	Verwendung von Fusionsproteinen, deren N-terminaler Anteil aus einem Hirudinderivat besteht, zur Herstellung rekombinanter Proteine über Sekretion durch Hefen
US7638618B2 (en)	2001-02-20	2009-12-29	Sanofi-Aventis Deutschland Gmbh	Nucleic acids encoding a hirudin and pro-insulin as superscretable peptides and for parallel improvement of the exported forms of one or more polypeptides of interest
ES2322945T5 (es) *	2001-12-21	2012-10-17	Immunex Corporation	Métodos para purficación de proteína
US7795205B2 (en)	2004-04-12	2010-09-14	Canyon Pharmaceuticals, Inc.	Methods for effecting regression of tumor mass and size in a metastasized pancreatic tumor
RU2411959C2 (ru) *	2004-06-29	2011-02-20	Релайанс Лайф Сайенсиз Пвт Лтд	Способ получения безопасных в вирусном отношении биологических текучих сред
JP6845139B2 (ja) *	2014-12-15	2021-03-17	メルクパテントゲゼルシャフトミットベシュレンクテルハフツングＭｅｒｃｋＰａｔｅｎｔＧｅｓｅｌｌｓｃｈａｆｔｍｉｔｂｅｓｃｈｒａｅｎｋｔｅｒＨａｆｔｕｎｇ	粗製溶液からの標的分子捕捉
CN107353338B (zh) *	2017-07-27	2020-11-17	宁波博睿修存生物科技有限公司	一种分离水蛭素发酵液中色素分子的方法
CN113984936A (zh) *	2021-12-09	2022-01-28	车延柠	一种水蛭酶解多肽的lc-ms特征图谱、检测方法及其应用

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
ES8207217A1 (es) *	1980-10-09	1982-09-01	Hoechst Ag	Procedimiento para la preparacion de un inactivador de alfa-amilasa
DE3342139A1 (de) *	1983-11-22	1985-05-30	Ciba-Geigy Ag, Basel	Desulfatohirudine, verfahren zu ihrer herstellung und pharmazeutische mittel
DE3438296A1 (de) *	1984-04-18	1985-11-07	Hoechst Ag, 6230 Frankfurt	Neue polypeptide mit blutgerinnungshemmender wirkung, verfahren zu deren herstellung bzw. gewinnung, deren verwendung und diese enthaltende mittel
DE3429430A1 (de) *	1984-08-10	1986-02-20	Hoechst Ag, 6230 Frankfurt	Gentechnologisches verfahren zur herstellung von hirudin und mittel zur durchfuehrung dieses verfahrens
US4616078A (en) *	1985-04-08	1986-10-07	Eli Lilly And Company	Process for purifying proinsulin-like materials
FR2593518B1 (fr) *	1985-05-02	1989-09-08	Transgene Sa	Vecteurs d'expression et de secretion de l'hirudine par les levures transformees
US4617376A (en) *	1985-07-01	1986-10-14	Eli Lilly And Company	Process for recovering glucagon from pancreas glands
DE3689525D1 (de) *	1985-07-17	1994-02-24	Hoechst Ag	Neue Polypeptide mit blutgerinnungshemmender Wirkung, Verfahren zu deren Herstellung bzw. Gewinnung, deren Verwendung und diese enthaltende Mittel.
DE3526995A1 (de) *	1985-07-27	1987-02-05	Hoechst Ag	Fusionsproteine, verfahren zu ihrer herstellung und ihre verwendung

1987
- 1987-11-13 DE DE19873738541 patent/DE3738541A1/de not_active Withdrawn
1988
- 1988-11-02 AT AT88118182T patent/ATE100109T1/de not_active IP Right Cessation
- 1988-11-02 ES ES88118182T patent/ES2049238T3/es not_active Expired - Lifetime
- 1988-11-02 EP EP88118182A patent/EP0316650B1/de not_active Expired - Lifetime
- 1988-11-02 DE DE88118182T patent/DE3887095D1/de not_active Expired - Lifetime
- 1988-11-10 PT PT88975A patent/PT88975B/pt not_active IP Right Cessation
- 1988-11-11 JP JP63284093A patent/JP2756279B2/ja not_active Expired - Lifetime
- 1988-11-11 DK DK198806306A patent/DK173366B1/da not_active IP Right Cessation
- 1988-11-11 IE IE339388A patent/IE62538B1/en not_active IP Right Cessation
1990
- 1990-09-27 US US07/590,581 patent/US5095092A/en not_active Expired - Lifetime

Also Published As

Publication number	Publication date
DK630688D0 (da)	1988-11-11
ES2049238T3 (es)	1994-04-16
EP0316650A2 (de)	1989-05-24
DE3738541A1 (de)	1989-05-24
ATE100109T1 (de)	1994-01-15
JP2756279B2 (ja)	1998-05-25
DK173366B1 (da)	2000-08-14
US5095092A (en)	1992-03-10
IE883393L (en)	1989-05-13
EP0316650B1 (de)	1994-01-12
IE62538B1 (en)	1995-02-08
JPH01165599A (ja)	1989-06-29
DK630688A (da)	1989-05-14
PT88975B (pt)	1994-11-30
EP0316650A3 (en)	1990-07-25
DE3887095D1 (de)	1994-02-24

Legal Events

Date

Code

Title

Description

1994-11-30

FG3A

Patent granted, date of granting

Effective date: 19940524

2003-01-31

PC3A

Transfer or assignment

Free format text: SCHERING AKTIENGESELLSCHAFT DE

Effective date: 20021128

2009-06-02

MM4A

Annulment/lapse due to non-payment of fees, searched and examined patent

Free format text: MAXIMUM VALIDITY LIMIT REACHED

Effective date: 20090524

Publication	Publication Date	Title
PT88975A (pt)	1989-11-30	Processo para o isolamento e purificacao de hirudina
BR8002149A (pt)	1981-03-10	Tecto-silicato poroso cristalino,composicao compreendendo o mesmo,e processo para conversao de compostos organicos
PT81190B (pt)	1987-09-30	Processo para o isolamento e purificacao de podofilotoxina
SE8307134D0 (sv)	1983-12-22	A process for purifying anthracyclinonic glycosides by selective adsorption on resins
DK530587D0 (da)	1987-10-09	Fremgangsmaade til oprensning af toxinkonjugater
DK575184D0 (da)	1984-12-04	Fremgangsmaade til oprensning af factor viii.c samt farmaceutisk praeparat indeholdende denne
DK0474213T3 (da)	1996-01-15	Fremgangsmåde til chromatografisk rensning af insuliner
DE3064133D1 (en)	1983-08-18	Purification of human growth hormone
ITFI920078A1 (it)	1993-09-27	Processo per l'isolamento di fattore ix, fattore x e fattore ii altamente purificati dal complesso protrombinico o dal plasma umano
NO941957L (no)	1994-11-29	Fremgangsmåte for fremstilling av eddiksyre ved kontrollert oksydasjon ev etan
IT1034702B (it)	1979-10-10	Processo per la carbossilazione di substrati organici con anidri de carbonica.
DE3270483D1 (en)	1986-05-15	Process for the purification of organic solutions
CH612160A5 (en)	1979-07-13	Process for the biological purification of effluent
BR8603221A (pt)	1987-02-24	Processo de cloracao seletiva de compostos fenolicos
FI941432L (fi)	1994-09-30	Menetelmä puolisynteettisten glydosaminoglykaanien valmistamiseksi, jotka sisältävät nukleofiilisillä radikaaleilla 3-asemassa substituoitua alfa-L-galakturonihappoa
SE7704110L (sv)	1977-10-09	Forfarande for framstellning och rening av sekretin
ES2046437T3 (es)	1994-02-01	Procedimiento para la separacion de cobre de unas soluciones acuosas de reaccion basica.
ZA784822B (en)	1979-08-29	Process for the purification of effluent which contains calcium and organic contaminants
DK165835C (da)	1993-06-21	Fremgangsmaade til isolering af antibiotika s541-forbindelser
BE846769A (fr)	1977-01-17	Procede de purification de solvants de la classe des hydrocarbures chlores
IT7831041A0 (it)	1978-12-20	Processo per la purificazione di olefine.
BR7900056A (pt)	1979-08-14	Processo para o isolamento de heparina pura
NO880449D0 (no)	1988-02-02	Fremgangsmaate ved hydrogenering av en umettet organisk forbindelse.
BR8406287A (pt)	1985-10-01	Processo para epoxidacao de pelo menos um composto poliol organico,contendo pelo menos um par de grupos hidroxi vicinais
JPS5398989A (en)	1978-08-29	Purification of cephalosporin compounds