RO68328B1 - Process for preparing amylolytic enzyme - Google Patents
Process for preparing amylolytic enzymeInfo
- Publication number
- RO68328B1 RO68328B1 RO92546A RO9254677A RO68328B1 RO 68328 B1 RO68328 B1 RO 68328B1 RO 92546 A RO92546 A RO 92546A RO 9254677 A RO9254677 A RO 9254677A RO 68328 B1 RO68328 B1 RO 68328B1
- Authority
- RO
- Romania
- Prior art keywords
- subjected
- cacl2
- amylolytic enzyme
- preparing
- solution
- Prior art date
Links
- 102000004190 Enzymes Human genes 0.000 title abstract 2
- 108090000790 Enzymes Proteins 0.000 title abstract 2
- 230000003625 amylolytic effect Effects 0.000 title abstract 2
- 238000004519 manufacturing process Methods 0.000 title 1
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 abstract 6
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 abstract 4
- 239000001110 calcium chloride Substances 0.000 abstract 4
- 229910001628 calcium chloride Inorganic materials 0.000 abstract 4
- 235000011148 calcium chloride Nutrition 0.000 abstract 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 abstract 2
- 239000002244 precipitate Substances 0.000 abstract 2
- 239000006228 supernatant Substances 0.000 abstract 2
- 241000237852 Mollusca Species 0.000 abstract 1
- 241000238367 Mya arenaria Species 0.000 abstract 1
- 238000005119 centrifugation Methods 0.000 abstract 1
- 238000000502 dialysis Methods 0.000 abstract 1
- 238000000265 homogenisation Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 239000011780 sodium chloride Substances 0.000 abstract 1
Landscapes
- Enzymes And Modification Thereof (AREA)
Abstract
Procedeu de obtinere a unei enzime amilolitice, caracterizat prin aceea ca, molustele marine Mya arenaria sunt supuse omogenizarii cu solutie de CaCl2 0,4% timp de o ora, dupa care omogenizatul obtinut se supune centrifugarii, supernatantul obtinut este tratat cu solutie de metanol 30%, dup care precipitatul obtinut este reluat cu solutie de CaCl2 0,4% se supune precipitarii cu NaCl 90% saturatie, se îndeparteaza precipitaul, iar supernatantul este supus dializarii fata de CaCl2 0,4%, se supunepreciaitarii cu metanol 30...60%, iar fractiunea precipitata este reluata cu solutie de CaCl2.Process for obtaining an amylolytic enzyme, characterized in that the Mya arenaria marine molluscs are subjected to homogenization with 0.4% CaCl2 solution for one hour, after which the obtained homogenate is subjected to centrifugation, the obtained supernatant is treated with methanol solution 30 %, after which the precipitate obtained is resumed with 0.4% CaCl2 solution, is precipitated with 90% saturation of NaCl, the precipitate is removed and the supernatant is subjected to dialysis against CaCl2 0.4%, subjected to methanol 30 ... 60%, and the precipitated fraction is resumed with CaCl2 solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RO7792546A RO68328A2 (en) | 1977-12-20 | 1977-12-20 | PROCESS FOR OBTAINING AN AMILOLITIVE ENZYME |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RO7792546A RO68328A2 (en) | 1977-12-20 | 1977-12-20 | PROCESS FOR OBTAINING AN AMILOLITIVE ENZYME |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RO68328B1 true RO68328B1 (en) | 1983-08-30 |
| RO68328A2 RO68328A2 (en) | 1983-09-26 |
Family
ID=20099117
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RO7792546A RO68328A2 (en) | 1977-12-20 | 1977-12-20 | PROCESS FOR OBTAINING AN AMILOLITIVE ENZYME |
Country Status (1)
| Country | Link |
|---|---|
| RO (1) | RO68328A2 (en) |
-
1977
- 1977-12-20 RO RO7792546A patent/RO68328A2/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| RO68328A2 (en) | 1983-09-26 |
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