RU2012103038A - Введение днк в растительные клетки - Google Patents
Введение днк в растительные клетки Download PDFInfo
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- RU2012103038A RU2012103038A RU2012103038/10A RU2012103038A RU2012103038A RU 2012103038 A RU2012103038 A RU 2012103038A RU 2012103038/10 A RU2012103038/10 A RU 2012103038/10A RU 2012103038 A RU2012103038 A RU 2012103038A RU 2012103038 A RU2012103038 A RU 2012103038A
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- Prior art keywords
- seed
- heterologous dna
- construct
- dna
- plant
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- 230000010354 integration Effects 0.000 title 1
- 108020004414 DNA Proteins 0.000 claims abstract 23
- 241000196324 Embryophyta Species 0.000 claims abstract 10
- 230000037452 priming Effects 0.000 claims abstract 8
- 108020005202 Viral DNA Proteins 0.000 claims abstract 7
- 210000004027 cell Anatomy 0.000 claims abstract 5
- 210000004602 germ cell Anatomy 0.000 claims abstract 5
- 150000007523 nucleic acids Chemical group 0.000 claims abstract 5
- 108091028043 Nucleic acid sequence Proteins 0.000 claims abstract 4
- 108010026228 mRNA guanylyltransferase Proteins 0.000 claims abstract 4
- 235000007688 Lycopersicon esculentum Nutrition 0.000 claims abstract 2
- 240000003768 Solanum lycopersicum Species 0.000 claims abstract 2
- 108700005077 Viral Genes Proteins 0.000 claims abstract 2
- 241000700605 Viruses Species 0.000 claims abstract 2
- 108090000623 proteins and genes Proteins 0.000 claims abstract 2
- 102000004169 proteins and genes Human genes 0.000 claims abstract 2
- 230000010076 replication Effects 0.000 claims abstract 2
- 230000007226 seed germination Effects 0.000 claims abstract 2
- 230000035040 seed growth Effects 0.000 claims abstract 2
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 1
- 239000002253 acid Substances 0.000 claims 1
- 239000007864 aqueous solution Substances 0.000 claims 1
- 230000035784 germination Effects 0.000 claims 1
- 239000011159 matrix material Substances 0.000 claims 1
- -1 nucleic acid sequence acids Chemical class 0.000 claims 1
- 239000007787 solid Substances 0.000 claims 1
- 230000008961 swelling Effects 0.000 claims 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
- C12N15/8203—Virus mediated transformation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
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- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Pharmacology & Pharmacy (AREA)
- Medicinal Chemistry (AREA)
- Virology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
1. Способ введения гетерологичной ДНК, по меньшей мере, в одну клетку зародыша растительного семени, включающий контактирование растительного семени со средой прайминга, содержащей вирусную ДНК конструкцию, содержащую гетерологичную ДНК, в условиях, позволяющих прайминг, с получением таким образом зародыша семени, содержащего гетерологичную ДНК.2. Способ по п.1, дополнительно включающий стадию обеспечения подходящих условий для дальнейшего прорастания семени и роста для получения растения или его части, содержащих гетерологичную ДНК.3. Способ по п.1, где вирусная ДНК конструкция, содержит вирусные гены или их части, обеспечивая репликацию и бессимптомное распределение конструкции в смежных растительных клетках.4. Способ по п.3, где ДНК конструкция представляет собой конструкцию на основе геминивируса.5. Способ по п.4, где ДНК конструкция содержит гетерологичную ДНК, фланкированную несмежными последовательностями нуклеиновых кислот, кодирующих репликазу геминивируса или репликаза-ассоциированные белки.6. Способ по любому из пп.4 и 5, где геминивирус представляет собой вирус желтой курчавости листьев томата (TYLCV).7. Способ по п.6, где ДНК конструкция представляет собой конструкцию на основе геминивируса, выбранную из группы, содержащей IL-60, имеющей последовательность нуклеиновых кислот, представленную в SEQ OD NO:1, и IL-60-BS, имеющей последовательность нуклеиновых кислот, представленную в SEQ ID NO:2.8. Способ по п.1, где гетерологичная ДНК кратковременно экспрессируется, по меньшей мере, в одной клетке зародыша семени и клетках, происходящих от нее.9. Способ по п.1, где гетерологичная ДНК вводится в геном, по меньшей мере, одной
Claims (14)
1. Способ введения гетерологичной ДНК, по меньшей мере, в одну клетку зародыша растительного семени, включающий контактирование растительного семени со средой прайминга, содержащей вирусную ДНК конструкцию, содержащую гетерологичную ДНК, в условиях, позволяющих прайминг, с получением таким образом зародыша семени, содержащего гетерологичную ДНК.
2. Способ по п.1, дополнительно включающий стадию обеспечения подходящих условий для дальнейшего прорастания семени и роста для получения растения или его части, содержащих гетерологичную ДНК.
3. Способ по п.1, где вирусная ДНК конструкция, содержит вирусные гены или их части, обеспечивая репликацию и бессимптомное распределение конструкции в смежных растительных клетках.
4. Способ по п.3, где ДНК конструкция представляет собой конструкцию на основе геминивируса.
5. Способ по п.4, где ДНК конструкция содержит гетерологичную ДНК, фланкированную несмежными последовательностями нуклеиновых кислот, кодирующих репликазу геминивируса или репликаза-ассоциированные белки.
6. Способ по любому из пп.4 и 5, где геминивирус представляет собой вирус желтой курчавости листьев томата (TYLCV).
7. Способ по п.6, где ДНК конструкция представляет собой конструкцию на основе геминивируса, выбранную из группы, содержащей IL-60, имеющей последовательность нуклеиновых кислот, представленную в SEQ OD NO:1, и IL-60-BS, имеющей последовательность нуклеиновых кислот, представленную в SEQ ID NO:2.
8. Способ по п.1, где гетерологичная ДНК кратковременно экспрессируется, по меньшей мере, в одной клетке зародыша семени и клетках, происходящих от нее.
9. Способ по п.1, где гетерологичная ДНК вводится в геном, по меньшей мере, одной клетки зародыша семени и клеток, происходящих от нее.
10. Способ по п.1, где среда прайминга имеет водный потенциал, обеспечивающий набухание семени, но не прорастание зародышевого корешка.
11. Способ по п.10, где среда прайминга представляет собой водный раствор.
12. Способ по п.11, где среда прайминга представляет собой твердую матрицу.
13. Семя, которое содержит вирусную ДНК конструкцию, где гетерологичная ДНК вводится в семя путем контактирования вирусной ДНК конструкции, содержащую гетерологичную ДНК, в условиях, позволяющих прайминг.
14. Растение или его часть, полученное от семени по п.13, где растение или его часть содержат вирусную ДНК конструкцию, содержащую гетерологичную ДНК.
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US22162609P | 2009-06-30 | 2009-06-30 | |
| US61/221,626 | 2009-06-30 | ||
| US28743509P | 2009-12-17 | 2009-12-17 | |
| US61/287,435 | 2009-12-17 | ||
| PCT/IL2010/000526 WO2011001434A1 (en) | 2009-06-30 | 2010-06-30 | Introducing dna into plant cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| RU2012103038A true RU2012103038A (ru) | 2013-08-10 |
Family
ID=42790525
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2012103038/10A RU2012103038A (ru) | 2009-06-30 | 2010-06-30 | Введение днк в растительные клетки |
Country Status (10)
| Country | Link |
|---|---|
| US (2) | US8975470B2 (ru) |
| EP (1) | EP2449110B1 (ru) |
| JP (1) | JP2012531924A (ru) |
| CN (1) | CN102575258A (ru) |
| AU (1) | AU2010267537B2 (ru) |
| CA (1) | CA2766918A1 (ru) |
| MX (1) | MX2012000271A (ru) |
| RU (1) | RU2012103038A (ru) |
| SG (1) | SG177412A1 (ru) |
| WO (1) | WO2011001434A1 (ru) |
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| US8962584B2 (en) | 2009-10-14 | 2015-02-24 | Yissum Research Development Company Of The Hebrew University Of Jerusalem, Ltd. | Compositions for controlling Varroa mites in bees |
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| US10683505B2 (en) | 2013-01-01 | 2020-06-16 | Monsanto Technology Llc | Methods of introducing dsRNA to plant seeds for modulating gene expression |
| UA118841C2 (uk) * | 2013-01-01 | 2019-03-25 | Ей.Бі. СІДС ЛТД. | Спосіб формування рослини, яка має підвищену стійкість до комахи-шкідника |
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-
2010
- 2010-06-30 WO PCT/IL2010/000526 patent/WO2011001434A1/en not_active Ceased
- 2010-06-30 US US13/381,103 patent/US8975470B2/en not_active Expired - Fee Related
- 2010-06-30 AU AU2010267537A patent/AU2010267537B2/en not_active Ceased
- 2010-06-30 EP EP10744736.9A patent/EP2449110B1/en not_active Not-in-force
- 2010-06-30 RU RU2012103038/10A patent/RU2012103038A/ru not_active Application Discontinuation
- 2010-06-30 JP JP2012519117A patent/JP2012531924A/ja active Pending
- 2010-06-30 CA CA2766918A patent/CA2766918A1/en not_active Abandoned
- 2010-06-30 CN CN2010800384590A patent/CN102575258A/zh active Pending
- 2010-06-30 SG SG2011097094A patent/SG177412A1/en unknown
- 2010-06-30 MX MX2012000271A patent/MX2012000271A/es not_active Application Discontinuation
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2015
- 2015-03-08 US US14/641,395 patent/US20150232875A1/en not_active Abandoned
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| Publication number | Publication date |
|---|---|
| WO2011001434A1 (en) | 2011-01-06 |
| EP2449110B1 (en) | 2017-05-31 |
| MX2012000271A (es) | 2012-09-07 |
| AU2010267537B2 (en) | 2014-05-22 |
| EP2449110A1 (en) | 2012-05-09 |
| US20120185967A1 (en) | 2012-07-19 |
| SG177412A1 (en) | 2012-02-28 |
| CN102575258A (zh) | 2012-07-11 |
| AU2010267537A1 (en) | 2012-02-09 |
| US20150232875A1 (en) | 2015-08-20 |
| JP2012531924A (ja) | 2012-12-13 |
| US8975470B2 (en) | 2015-03-10 |
| CA2766918A1 (en) | 2011-01-06 |
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