RU2577143C2 - Детекция aad-1 объекта das-40278-9 - Google Patents

Детекция aad-1 объекта das-40278-9 Download PDF

Info

Publication number: RU2577143C2
Authority: RU; Russia
Prior art keywords: corn; seq; dna; sequence; specified
Prior art date: 2009-08-19

Application number

RU2012110253/10A

Other languages

English (en)

Russian (ru)

Other versions

RU2012110253A (ru

Inventor

Юньсин Кори ЦУЙ

Томас Уилльям ГРИН

Стефен НОВАК

Нин Чжоу

Original Assignee

ДАУ АГРОСАЙЕНСИЗ ЭлЭлСи

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2009-08-19

Filing date

2010-08-18

Publication date

2016-03-10

2010-08-18 Application filed by ДАУ АГРОСАЙЕНСИЗ ЭлЭлСи filed Critical ДАУ АГРОСАЙЕНСИЗ ЭлЭлСи

2013-09-27 Publication of RU2012110253A publication Critical patent/RU2012110253A/ru

2016-03-10 Application granted granted Critical

2016-03-10 Publication of RU2577143C2 publication Critical patent/RU2577143C2/ru

Links

238000001514 detection method Methods 0.000 title description 18
101100442689 Caenorhabditis elegans hdl-1 gene Proteins 0.000 title description 4
238000000034 method Methods 0.000 claims abstract description 99
239000000523 sample Substances 0.000 claims abstract description 86
230000009261 transgenic effect Effects 0.000 claims abstract description 37
241001057636 Dracaena deremensis Species 0.000 claims abstract description 22
239000007850 fluorescent dye Substances 0.000 claims abstract 5
240000008042 Zea mays Species 0.000 claims description 94
235000002017 Zea mays subsp mays Nutrition 0.000 claims description 90
108020004414 DNA Proteins 0.000 claims description 82
235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 45
235000005822 corn Nutrition 0.000 claims description 45
235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 claims description 44
235000009973 maize Nutrition 0.000 claims description 44
108091093088 Amplicon Proteins 0.000 claims description 40
230000000295 complement effect Effects 0.000 claims description 19
238000003753 real-time PCR Methods 0.000 claims description 12
108010028143 Dioxygenases Proteins 0.000 claims description 3
238000013461 design Methods 0.000 claims description 3
FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 2
238000004445 quantitative analysis Methods 0.000 claims 1
108090000623 proteins and genes Proteins 0.000 abstract description 41
230000000694 effects Effects 0.000 abstract description 2
238000010276 construction Methods 0.000 abstract 1
239000000126 substance Substances 0.000 abstract 1
239000013615 primer Substances 0.000 description 74
241000196324 Embryophyta Species 0.000 description 54
150000007523 nucleic acids Chemical class 0.000 description 38
108091028043 Nucleic acid sequence Proteins 0.000 description 34
238000004458 analytical method Methods 0.000 description 30
238000009396 hybridization Methods 0.000 description 28
238000003199 nucleic acid amplification method Methods 0.000 description 28
102000039446 nucleic acids Human genes 0.000 description 28
108020004707 nucleic acids Proteins 0.000 description 28
230000003321 amplification Effects 0.000 description 27
238000003752 polymerase chain reaction Methods 0.000 description 25
239000004009 herbicide Substances 0.000 description 22
239000002987 primer (paints) Substances 0.000 description 20
125000003729 nucleotide group Chemical group 0.000 description 17
239000002773 nucleotide Substances 0.000 description 16
238000006243 chemical reaction Methods 0.000 description 13
230000002363 herbicidal effect Effects 0.000 description 11
108700019146 Transgenes Proteins 0.000 description 10
108091092584 GDNA Proteins 0.000 description 8
108091034117 Oligonucleotide Proteins 0.000 description 8
238000002866 fluorescence resonance energy transfer Methods 0.000 description 8
239000012634 fragment Substances 0.000 description 8
238000004519 manufacturing process Methods 0.000 description 8
238000009395 breeding Methods 0.000 description 7
230000001488 breeding effect Effects 0.000 description 7
235000013305 food Nutrition 0.000 description 7
239000003112 inhibitor Substances 0.000 description 7
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
108010051210 beta-Fructofuranosidase Proteins 0.000 description 6
238000003780 insertion Methods 0.000 description 6
230000037431 insertion Effects 0.000 description 6
241000238631 Hexapoda Species 0.000 description 5
239000000203 mixture Substances 0.000 description 5
238000003976 plant breeding Methods 0.000 description 5
150000003839 salts Chemical class 0.000 description 5
102000016928 DNA-directed DNA polymerase Human genes 0.000 description 4
108010014303 DNA-directed DNA polymerase Proteins 0.000 description 4
238000012408 PCR amplification Methods 0.000 description 4
230000009418 agronomic effect Effects 0.000 description 4
238000003556 assay Methods 0.000 description 4
238000009402 cross-breeding Methods 0.000 description 4
238000011161 development Methods 0.000 description 4
230000018109 developmental process Effects 0.000 description 4
230000014509 gene expression Effects 0.000 description 4
230000002068 genetic effect Effects 0.000 description 4
239000003550 marker Substances 0.000 description 4
239000003921 oil Substances 0.000 description 4
108091033319 polynucleotide Proteins 0.000 description 4
102000040430 polynucleotide Human genes 0.000 description 4
239000002157 polynucleotide Substances 0.000 description 4
238000012545 processing Methods 0.000 description 4
230000010153 self-pollination Effects 0.000 description 4
238000012360 testing method Methods 0.000 description 4
238000005406 washing Methods 0.000 description 4
241000219194 Arabidopsis Species 0.000 description 3
102000053602 DNA Human genes 0.000 description 3
108020001019 DNA Primers Proteins 0.000 description 3
230000004544 DNA amplification Effects 0.000 description 3
239000003155 DNA primer Substances 0.000 description 3
KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 3
102000004190 Enzymes Human genes 0.000 description 3
108090000790 Enzymes Proteins 0.000 description 3
108020001991 Protoporphyrinogen Oxidase Proteins 0.000 description 3
239000012472 biological sample Substances 0.000 description 3
230000008859 change Effects 0.000 description 3
239000003153 chemical reaction reagent Substances 0.000 description 3
238000010367 cloning Methods 0.000 description 3
235000021306 genetically modified maize Nutrition 0.000 description 3
239000001573 invertase Substances 0.000 description 3
235000011073 invertase Nutrition 0.000 description 3
102000004169 proteins and genes Human genes 0.000 description 3
230000000306 recurrent effect Effects 0.000 description 3
230000001568 sexual effect Effects 0.000 description 3
239000011780 sodium chloride Substances 0.000 description 3
238000011895 specific detection Methods 0.000 description 3
210000001519 tissue Anatomy 0.000 description 3
IAJOBQBIJHVGMQ-UHFFFAOYSA-N 2-amino-4-[hydroxy(methyl)phosphoryl]butanoic acid Chemical compound CP(O)(=O)CCC(N)C(O)=O IAJOBQBIJHVGMQ-UHFFFAOYSA-N 0.000 description 2
CAAMSDWKXXPUJR-UHFFFAOYSA-N 3,5-dihydro-4H-imidazol-4-one Chemical compound O=C1CNC=N1 CAAMSDWKXXPUJR-UHFFFAOYSA-N 0.000 description 2
LZZYPRNAOMGNLH-UHFFFAOYSA-M Cetrimonium bromide Chemical compound [Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C LZZYPRNAOMGNLH-UHFFFAOYSA-M 0.000 description 2
102000016680 Dioxygenases Human genes 0.000 description 2
239000005561 Glufosinate Substances 0.000 description 2
235000010469 Glycine max Nutrition 0.000 description 2
239000005562 Glyphosate Substances 0.000 description 2
108020004711 Nucleic Acid Probes Proteins 0.000 description 2
108020005187 Oligonucleotide Probes Proteins 0.000 description 2
238000010222 PCR analysis Methods 0.000 description 2
108091093037 Peptide nucleic acid Proteins 0.000 description 2
102100029028 Protoporphyrinogen oxidase Human genes 0.000 description 2
DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 2
235000007244 Zea mays Nutrition 0.000 description 2
230000008901 benefit Effects 0.000 description 2
210000004027 cell Anatomy 0.000 description 2
235000013339 cereals Nutrition 0.000 description 2
150000001875 compounds Chemical class 0.000 description 2
238000004925 denaturation Methods 0.000 description 2
230000036425 denaturation Effects 0.000 description 2
235000003869 genetically modified organism Nutrition 0.000 description 2
XDDAORKBJWWYJS-UHFFFAOYSA-N glyphosate Chemical compound OC(=O)CNCP(O)(O)=O XDDAORKBJWWYJS-UHFFFAOYSA-N 0.000 description 2
229940097068 glyphosate Drugs 0.000 description 2
230000010354 integration Effects 0.000 description 2
239000000463 material Substances 0.000 description 2
239000011159 matrix material Substances 0.000 description 2
239000002853 nucleic acid probe Substances 0.000 description 2
239000002751 oligonucleotide probe Substances 0.000 description 2
238000009401 outcrossing Methods 0.000 description 2
239000013612 plasmid Substances 0.000 description 2
230000010287 polarization Effects 0.000 description 2
238000003908 quality control method Methods 0.000 description 2
238000011002 quantification Methods 0.000 description 2
230000002285 radioactive effect Effects 0.000 description 2
NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 2
239000001509 sodium citrate Substances 0.000 description 2
238000013518 transcription Methods 0.000 description 2
230000035897 transcription Effects 0.000 description 2
230000009466 transformation Effects 0.000 description 2
MPKRXENUURLWTO-UHFFFAOYSA-N 1-phenyl-7,9-dihydro-3h-purine-2,6,8-trione Chemical compound O=C1C=2NC(=O)NC=2NC(=O)N1C1=CC=CC=C1 MPKRXENUURLWTO-UHFFFAOYSA-N 0.000 description 1
239000005631 2,4-Dichlorophenoxyacetic acid Substances 0.000 description 1
HXKWSTRRCHTUEC-UHFFFAOYSA-N 2,4-Dichlorophenoxyaceticacid Chemical compound OC(=O)C(Cl)OC1=CC=C(Cl)C=C1 HXKWSTRRCHTUEC-UHFFFAOYSA-N 0.000 description 1
GQQIAHNFBAFBCS-UHFFFAOYSA-N 2-[2-chloro-5-(1,3-dioxo-4,5,6,7-tetrahydroisoindol-2-yl)-4-fluorophenoxy]acetic acid Chemical compound C1=C(Cl)C(OCC(=O)O)=CC(N2C(C3=C(CCCC3)C2=O)=O)=C1F GQQIAHNFBAFBCS-UHFFFAOYSA-N 0.000 description 1
OOLBCHYXZDXLDS-UHFFFAOYSA-N 2-[4-(2,4-dichlorophenoxy)phenoxy]propanoic acid Chemical compound C1=CC(OC(C)C(O)=O)=CC=C1OC1=CC=C(Cl)C=C1Cl OOLBCHYXZDXLDS-UHFFFAOYSA-N 0.000 description 1
OSBLTNPMIGYQGY-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;2-[2-[bis(carboxymethyl)amino]ethyl-(carboxymethyl)amino]acetic acid;boric acid Chemical compound OB(O)O.OCC(N)(CO)CO.OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O OSBLTNPMIGYQGY-UHFFFAOYSA-N 0.000 description 1
YHKBGVDUSSWOAB-UHFFFAOYSA-N 2-chloro-3-{2-chloro-5-[4-(difluoromethyl)-3-methyl-5-oxo-4,5-dihydro-1H-1,2,4-triazol-1-yl]-4-fluorophenyl}propanoic acid Chemical compound O=C1N(C(F)F)C(C)=NN1C1=CC(CC(Cl)C(O)=O)=C(Cl)C=C1F YHKBGVDUSSWOAB-UHFFFAOYSA-N 0.000 description 1
CDUVSERIDNVFDD-UHFFFAOYSA-N 2-pyrimidin-2-ylbenzenecarbothioic s-acid Chemical class OC(=S)C1=CC=CC=C1C1=NC=CC=N1 CDUVSERIDNVFDD-UHFFFAOYSA-N 0.000 description 1
UPMXNNIRAGDFEH-UHFFFAOYSA-N 3,5-dibromo-4-hydroxybenzonitrile Chemical compound OC1=C(Br)C=C(C#N)C=C1Br UPMXNNIRAGDFEH-UHFFFAOYSA-N 0.000 description 1
108010020183 3-phosphoshikimate 1-carboxyvinyltransferase Proteins 0.000 description 1
101150111419 ATH1 gene Proteins 0.000 description 1
108010000700 Acetolactate synthase Proteins 0.000 description 1
102000007698 Alcohol dehydrogenase Human genes 0.000 description 1
108010021809 Alcohol dehydrogenase Proteins 0.000 description 1
108010007730 Apyrase Proteins 0.000 description 1
102000007347 Apyrase Human genes 0.000 description 1
241000219195 Arabidopsis thaliana Species 0.000 description 1
101100433747 Arabidopsis thaliana ABCA2 gene Proteins 0.000 description 1
101100288148 Arabidopsis thaliana KNAT5 gene Proteins 0.000 description 1
101100135611 Arabidopsis thaliana PAP12 gene Proteins 0.000 description 1
101150002428 Atoh1 gene Proteins 0.000 description 1
239000005489 Bromoxynil Substances 0.000 description 1
101100057132 Candida albicans (strain SC5314 / ATCC MYA-2876) ATC1 gene Proteins 0.000 description 1
229920000742 Cotton Polymers 0.000 description 1
IGXWBGJHJZYPQS-SSDOTTSWSA-N D-Luciferin Chemical compound OC(=O)[C@H]1CSC(C=2SC3=CC=C(O)C=C3N=2)=N1 IGXWBGJHJZYPQS-SSDOTTSWSA-N 0.000 description 1
239000003298 DNA probe Substances 0.000 description 1
238000001712 DNA sequencing Methods 0.000 description 1
CYCGRDQQIOGCKX-UHFFFAOYSA-N Dehydro-luciferin Natural products OC(=O)C1=CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 CYCGRDQQIOGCKX-UHFFFAOYSA-N 0.000 description 1
239000005504 Dicamba Substances 0.000 description 1
239000005506 Diclofop Substances 0.000 description 1
QNXAVFXEJCPCJO-UHFFFAOYSA-N Diclosulam Chemical compound N=1N2C(OCC)=NC(F)=CC2=NC=1S(=O)(=O)NC1=C(Cl)C=CC=C1Cl QNXAVFXEJCPCJO-UHFFFAOYSA-N 0.000 description 1
208000035240 Disease Resistance Diseases 0.000 description 1
238000002965 ELISA Methods 0.000 description 1
BJGNCJDXODQBOB-UHFFFAOYSA-N Fivefly Luciferin Natural products OC(=O)C1CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 BJGNCJDXODQBOB-UHFFFAOYSA-N 0.000 description 1
RXCPQSJAVKGONC-UHFFFAOYSA-N Flumetsulam Chemical compound N1=C2N=C(C)C=CN2N=C1S(=O)(=O)NC1=C(F)C=CC=C1F RXCPQSJAVKGONC-UHFFFAOYSA-N 0.000 description 1
239000005532 Flumioxazine Substances 0.000 description 1
241000233866 Fungi Species 0.000 description 1
244000068988 Glycine max Species 0.000 description 1
238000009015 Human TaqMan MicroRNA Assay kit Methods 0.000 description 1
229930010555 Inosine Natural products 0.000 description 1
UGQMRVRMYYASKQ-KQYNXXCUSA-N Inosine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C2=NC=NC(O)=C2N=C1 UGQMRVRMYYASKQ-KQYNXXCUSA-N 0.000 description 1
101001091419 Ipomoea batatas Polyphenol oxidase I, chloroplastic Proteins 0.000 description 1
108090001090 Lectins Proteins 0.000 description 1
108060001084 Luciferase Proteins 0.000 description 1
239000005089 Luciferase Substances 0.000 description 1
DDWFXDSYGUXRAY-UHFFFAOYSA-N Luciferin Natural products CCc1c(C)c(CC2NC(=O)C(=C2C=C)C)[nH]c1Cc3[nH]c4C(=C5/NC(CC(=O)O)C(C)C5CC(=O)O)CC(=O)c4c3C DDWFXDSYGUXRAY-UHFFFAOYSA-N 0.000 description 1
239000005578 Mesotrione Substances 0.000 description 1
239000005582 Metosulam Substances 0.000 description 1
VGHPMIFEKOFHHQ-UHFFFAOYSA-N Metosulam Chemical compound N1=C2N=C(OC)C=C(OC)N2N=C1S(=O)(=O)NC1=C(Cl)C=CC(C)=C1Cl VGHPMIFEKOFHHQ-UHFFFAOYSA-N 0.000 description 1
101001123254 Nicotiana tabacum Protoporphyrinogen oxidase, chloroplastic Proteins 0.000 description 1
102000008297 Nuclear Matrix-Associated Proteins Human genes 0.000 description 1
108010035916 Nuclear Matrix-Associated Proteins Proteins 0.000 description 1
239000005590 Oxyfluorfen Substances 0.000 description 1
OQMBBFQZGJFLBU-UHFFFAOYSA-N Oxyfluorfen Chemical compound C1=C([N+]([O-])=O)C(OCC)=CC(OC=2C(=CC(=CC=2)C(F)(F)F)Cl)=C1 OQMBBFQZGJFLBU-UHFFFAOYSA-N 0.000 description 1
238000009004 PCR Kit Methods 0.000 description 1
238000002944 PCR assay Methods 0.000 description 1
108010081996 Photosystem I Protein Complex Proteins 0.000 description 1
108010060806 Photosystem II Protein Complex Proteins 0.000 description 1
108020005120 Plant DNA Proteins 0.000 description 1
239000004952 Polyamide Substances 0.000 description 1
102000005135 Protoporphyrinogen oxidase Human genes 0.000 description 1
108020004518 RNA Probes Proteins 0.000 description 1
239000003391 RNA probe Substances 0.000 description 1
238000011529 RT qPCR Methods 0.000 description 1
241001292337 Sphingobium herbicidovorans Species 0.000 description 1
239000005618 Sulcotrione Substances 0.000 description 1
102000004523 Sulfate Adenylyltransferase Human genes 0.000 description 1
108010022348 Sulfate adenylyltransferase Proteins 0.000 description 1
239000008051 TBE buffer Substances 0.000 description 1
102100029373 Transcription factor ATOH1 Human genes 0.000 description 1
239000007983 Tris buffer Substances 0.000 description 1
241000607479 Yersinia pestis Species 0.000 description 1
241000482268 Zea mays subsp. mays Species 0.000 description 1
108010055615 Zein Proteins 0.000 description 1
JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 1
NUFNQYOELLVIPL-UHFFFAOYSA-N acifluorfen Chemical compound C1=C([N+]([O-])=O)C(C(=O)O)=CC(OC=2C(=CC(=CC=2)C(F)(F)F)Cl)=C1 NUFNQYOELLVIPL-UHFFFAOYSA-N 0.000 description 1
208000005652 acute fatty liver of pregnancy Diseases 0.000 description 1
IRLPACMLTUPBCL-FCIPNVEPSA-N adenosine-5'-phosphosulfate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@@H](CO[P@](O)(=O)OS(O)(=O)=O)[C@H](O)[C@H]1O IRLPACMLTUPBCL-FCIPNVEPSA-N 0.000 description 1
238000000246 agarose gel electrophoresis Methods 0.000 description 1
238000003149 assay kit Methods 0.000 description 1
230000001580 bacterial effect Effects 0.000 description 1
KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 1
239000004327 boric acid Substances 0.000 description 1
239000000872 buffer Substances 0.000 description 1
239000007853 buffer solution Substances 0.000 description 1
JEDYYFXHPAIBGR-UHFFFAOYSA-N butafenacil Chemical compound O=C1N(C)C(C(F)(F)F)=CC(=O)N1C1=CC=C(Cl)C(C(=O)OC(C)(C)C(=O)OCC=C)=C1 JEDYYFXHPAIBGR-UHFFFAOYSA-N 0.000 description 1
238000004113 cell culture Methods 0.000 description 1
239000005081 chemiluminescent agent Substances 0.000 description 1
210000000349 chromosome Anatomy 0.000 description 1
238000003776 cleavage reaction Methods 0.000 description 1
BIKACRYIQSLICJ-UHFFFAOYSA-N cloransulam-methyl Chemical group N=1N2C(OCC)=NC(F)=CC2=NC=1S(=O)(=O)NC1=C(Cl)C=CC=C1C(=O)OC BIKACRYIQSLICJ-UHFFFAOYSA-N 0.000 description 1
238000004590 computer program Methods 0.000 description 1
238000013270 controlled release Methods 0.000 description 1
238000007796 conventional method Methods 0.000 description 1
108010027183 cytochrome P-450 CYP76B1 (Helianthus tuberosus) Proteins 0.000 description 1
238000007405 data analysis Methods 0.000 description 1
238000003745 diagnosis Methods 0.000 description 1
IWEDIXLBFLAXBO-UHFFFAOYSA-N dicamba Chemical compound COC1=C(Cl)C=CC(Cl)=C1C(O)=O IWEDIXLBFLAXBO-UHFFFAOYSA-N 0.000 description 1
239000000539 dimer Substances 0.000 description 1
USIUVYZYUHIAEV-UHFFFAOYSA-N diphenyl ether Chemical class C=1C=CC=CC=1OC1=CC=CC=C1 USIUVYZYUHIAEV-UHFFFAOYSA-N 0.000 description 1
238000007848 endpoint PCR Methods 0.000 description 1
238000005516 engineering process Methods 0.000 description 1
230000007613 environmental effect Effects 0.000 description 1
239000002532 enzyme inhibitor Substances 0.000 description 1
ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 1
229960005542 ethidium bromide Drugs 0.000 description 1
238000000605 extraction Methods 0.000 description 1
230000035558 fertility Effects 0.000 description 1
239000000835 fiber Substances 0.000 description 1
WFZSZAXUALBVNX-UHFFFAOYSA-N flufenpyr Chemical compound O=C1C(C)=C(C(F)(F)F)C=NN1C1=CC(OCC(O)=O)=C(Cl)C=C1F WFZSZAXUALBVNX-UHFFFAOYSA-N 0.000 description 1
FOUWCSDKDDHKQP-UHFFFAOYSA-N flumioxazin Chemical compound FC1=CC=2OCC(=O)N(CC#C)C=2C=C1N(C1=O)C(=O)C2=C1CCCC2 FOUWCSDKDDHKQP-UHFFFAOYSA-N 0.000 description 1
238000012921 fluorescence analysis Methods 0.000 description 1
238000002875 fluorescence polarization Methods 0.000 description 1
BGZZWXTVIYUUEY-UHFFFAOYSA-N fomesafen Chemical compound C1=C([N+]([O-])=O)C(C(=O)NS(=O)(=O)C)=CC(OC=2C(=CC(=CC=2)C(F)(F)F)Cl)=C1 BGZZWXTVIYUUEY-UHFFFAOYSA-N 0.000 description 1
230000007062 hydrolysis Effects 0.000 description 1
238000006460 hydrolysis reaction Methods 0.000 description 1
208000015181 infectious disease Diseases 0.000 description 1
230000002401 inhibitory effect Effects 0.000 description 1
229960003786 inosine Drugs 0.000 description 1
238000002955 isolation Methods 0.000 description 1
-1 isoxaflutol Chemical compound 0.000 description 1
CONWAEURSVPLRM-UHFFFAOYSA-N lactofen Chemical compound C1=C([N+]([O-])=O)C(C(=O)OC(C)C(=O)OCC)=CC(OC=2C(=CC(=CC=2)C(F)(F)F)Cl)=C1 CONWAEURSVPLRM-UHFFFAOYSA-N 0.000 description 1
239000003446 ligand Substances 0.000 description 1
238000007403 mPCR Methods 0.000 description 1
230000014759 maintenance of location Effects 0.000 description 1
230000013011 mating Effects 0.000 description 1
238000005259 measurement Methods 0.000 description 1
KPUREKXXPHOJQT-UHFFFAOYSA-N mesotrione Chemical compound [O-][N+](=O)C1=CC(S(=O)(=O)C)=CC=C1C(=O)C1C(=O)CCCC1=O KPUREKXXPHOJQT-UHFFFAOYSA-N 0.000 description 1
230000011987 methylation Effects 0.000 description 1
238000007069 methylation reaction Methods 0.000 description 1
238000010369 molecular cloning Methods 0.000 description 1
239000003068 molecular probe Substances 0.000 description 1
OKCDZCGCKMTKMK-UHFFFAOYSA-N n-phenyl-2h-triazolo[4,5-d]pyrimidine-5-sulfonamide Chemical compound N=1C=C2NN=NC2=NC=1S(=O)(=O)NC1=CC=CC=C1 OKCDZCGCKMTKMK-UHFFFAOYSA-N 0.000 description 1
238000010606 normalization Methods 0.000 description 1
210000000299 nuclear matrix Anatomy 0.000 description 1
238000007899 nucleic acid hybridization Methods 0.000 description 1
ONDBSQWZWRGVIL-UHFFFAOYSA-N o-pyrimidin-2-yl benzenecarbothioate Chemical class C=1C=CC=CC=1C(=S)OC1=NC=CC=N1 ONDBSQWZWRGVIL-UHFFFAOYSA-N 0.000 description 1
238000004806 packaging method and process Methods 0.000 description 1
LCCNCVORNKJIRZ-UHFFFAOYSA-N parathion Chemical compound CCOP(=S)(OCC)OC1=CC=C([N+]([O-])=O)C=C1 LCCNCVORNKJIRZ-UHFFFAOYSA-N 0.000 description 1
108010001545 phytoene dehydrogenase Proteins 0.000 description 1
229920002647 polyamide Polymers 0.000 description 1
230000008569 process Effects 0.000 description 1
101150075980 psbA gene Proteins 0.000 description 1
238000012175 pyrosequencing Methods 0.000 description 1
238000011158 quantitative evaluation Methods 0.000 description 1
238000010791 quenching Methods 0.000 description 1
230000000171 quenching effect Effects 0.000 description 1
239000011541 reaction mixture Substances 0.000 description 1
230000008929 regeneration Effects 0.000 description 1
238000011069 regeneration method Methods 0.000 description 1
230000001105 regulatory effect Effects 0.000 description 1
238000007894 restriction fragment length polymorphism technique Methods 0.000 description 1
238000007363 ring formation reaction Methods 0.000 description 1
229920002477 rna polymer Polymers 0.000 description 1
230000007017 scission Effects 0.000 description 1
238000000926 separation method Methods 0.000 description 1
238000012163 sequencing technique Methods 0.000 description 1
239000000243 solution Substances 0.000 description 1
239000002904 solvent Substances 0.000 description 1
230000010473 stable expression Effects 0.000 description 1
238000010186 staining Methods 0.000 description 1
PQTBTIFWAXVEPB-UHFFFAOYSA-N sulcotrione Chemical compound ClC1=CC(S(=O)(=O)C)=CC=C1C(=O)C1C(=O)CCCC1=O PQTBTIFWAXVEPB-UHFFFAOYSA-N 0.000 description 1
OORLZFUTLGXMEF-UHFFFAOYSA-N sulfentrazone Chemical compound O=C1N(C(F)F)C(C)=NN1C1=CC(NS(C)(=O)=O)=C(Cl)C=C1Cl OORLZFUTLGXMEF-UHFFFAOYSA-N 0.000 description 1
230000001629 suppression Effects 0.000 description 1
239000000725 suspension Substances 0.000 description 1
238000012546 transfer Methods 0.000 description 1
230000001131 transforming effect Effects 0.000 description 1
YWBFPKPWMSWWEA-UHFFFAOYSA-O triazolopyrimidine Chemical compound BrC1=CC=CC(C=2N=C3N=CN[N+]3=C(NCC=3C=CN=CC=3)C=2)=C1 YWBFPKPWMSWWEA-UHFFFAOYSA-O 0.000 description 1
230000035899 viability Effects 0.000 description 1
239000011534 wash buffer Substances 0.000 description 1
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1

Images

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Genetics & Genomics (AREA)
Chemical & Material Sciences (AREA)
Engineering & Computer Science (AREA)
Zoology (AREA)
Bioinformatics & Cheminformatics (AREA)
Wood Science & Technology (AREA)
Organic Chemistry (AREA)
General Engineering & Computer Science (AREA)
Biomedical Technology (AREA)
Biotechnology (AREA)
Molecular Biology (AREA)
Microbiology (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Medicinal Chemistry (AREA)
Cell Biology (AREA)
Physics & Mathematics (AREA)
Biophysics (AREA)
Plant Pathology (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Apparatus Associated With Microorganisms And Enzymes (AREA)
Pretreatment Of Seeds And Plants (AREA)
Cultivation Of Plants (AREA)

RU2012110253/10A 2009-08-19 2010-08-18 Детекция aad-1 объекта das-40278-9 RU2577143C2 (ru)

Applications Claiming Priority (5)

Application Number	Priority Date	Filing Date	Title
US23524809P	2009-08-19	2009-08-19
US61/235,248		2009-08-19
US23736610P	2010-04-23	2010-04-23
US61/237,366		2010-04-23
PCT/US2010/045871 WO2011022471A1 (fr)	2009-08-19	2010-08-18	Détection dévénement aad-1 das-40278-9

Publications (2)

Publication Number	Publication Date
RU2012110253A RU2012110253A (ru)	2013-09-27
RU2577143C2 true RU2577143C2 (ru)	2016-03-10

Family

ID=43607318

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
RU2012110253/10A RU2577143C2 (ru)	2009-08-19	2010-08-18	Детекция aad-1 объекта das-40278-9

Country Status (14)

Country	Link
JP (1)	JP5771208B2 (fr)
KR (1)	KR101772638B1 (fr)
CN (1)	CN102575299B (fr)
AU (1)	AU2010284286B2 (fr)
CA (1)	CA2771581C (fr)
CL (1)	CL2012000410A1 (fr)
CO (1)	CO6511214A2 (fr)
IL (1)	IL218175A (fr)
IN (1)	IN2012DN01800A (fr)
MX (1)	MX336072B (fr)
NZ (1)	NZ598598A (fr)
RU (1)	RU2577143C2 (fr)
WO (1)	WO2011022471A1 (fr)
ZA (1)	ZA201201210B (fr)

Families Citing this family (58)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US8598413B2 (en) *	2009-08-19	2013-12-03	Dow AgroSciecnes, LLC.	AAD-1 event DAS-40278-9, related transgenic corn lines, event-specific identification thereof, and methods of weed control involving AAD-1
MX338249B (es)	2009-08-19	2016-04-08	Dow Agrosciences Llc	Evento das-40278-9 de ariloaxialcanoato dioxigenasa-1 lineas transgenicas de maiz relacionadas e identificacion de evento-especifico de las mismas.
TWI667347B (zh)	2010-12-15	2019-08-01	瑞士商先正達合夥公司	大豆品種ｓｙｈｔ０ｈ２及偵測其之組合物及方法
MX365442B (es) *	2014-03-20	2019-06-03	Monsanto Technology Llc	Evento de maiz transgenico mon 87419 y metodos para su uso.
GB201622007D0 (en)	2016-12-22	2017-02-08	And See Cambridge Display Tech Ltd Syngenta Participations Ag	Polymorphs
PL3558961T3 (pl)	2016-12-22	2021-06-28	Syngenta Participations Ag	Polimorfy
UY37623A (es)	2017-03-03	2018-09-28	Syngenta Participations Ag	Derivados de oxadiazol tiofeno fungicidas
CA3055680A1 (fr)	2017-03-31	2018-10-04	Syngenta Participations Ag	Compositions fongicides
WO2018177880A1 (fr)	2017-03-31	2018-10-04	Syngenta Participations Ag	Compositions fongicides
WO2018185013A1 (fr)	2017-04-03	2018-10-11	Syngenta Participations Ag	Dérivés d'oxadiazole microbiocides
BR112019020735B1 (pt)	2017-04-05	2023-12-05	Syngenta Participations Ag	Compostos derivados de oxadiazol microbiocidas e seu uso, composição agroquímica e método para controlar ou prevenir a infestação de plantas úteis por microrganismos fitopatogênicos
BR112019021019B1 (pt)	2017-04-05	2023-12-05	Syngenta Participations Ag	Compostos derivados de oxadiazol microbiocidas, composição agrícola, método para controlar ou prevenir a infestação de plantas úteis por microrganismos fitopatogênicos e uso de um composto derivado de oxadiazol
BR112019020734B1 (pt)	2017-04-05	2023-12-05	Syngenta Participations Ag	Compostos derivados de oxadiazol, composição agroquímica, método para controlar ou prevenir a infestação de plantas úteis por microrganismos fitopatogênicos e uso dos referidos compostos
BR112019020739B1 (pt)	2017-04-05	2023-12-19	Syngenta Participations Ag	Compostos derivados de oxadiazol microbiocidas e seu uso, composição agroquímica, método para controlar ou prevenir a infestação de plantas úteis por microrganismos fitopatogênicos
BR112019020819B1 (pt)	2017-04-05	2023-12-05	Syngenta Participations Ag	Composto de fórmula (i), composição agroquímica, método para controlar ou prevenir a infestação de plantas úteis por micro-organismos fitopatogênicos e uso de um composto de fórmula (i)
WO2018184984A1 (fr)	2017-04-05	2018-10-11	Syngenta Participations Ag	Dérivés d'oxadiazole microbiocides
WO2018185211A1 (fr)	2017-04-06	2018-10-11	Syngenta Participations Ag	Dérivés d'oxadiazole microbiocides
EP3630753A1 (fr)	2017-06-02	2020-04-08	Syngenta Participations AG	Dérivés d'oxadiazole microbiocides
WO2018219773A1 (fr)	2017-06-02	2018-12-06	Syngenta Participations Ag	Compositions fongicides
WO2018228896A1 (fr)	2017-06-14	2018-12-20	Syngenta Participations Ag	Compositions fongicides
KR20200022452A (ko)	2017-06-28	2020-03-03	신젠타 파티서페이션즈 아게	살진균 조성물
WO2019011929A1 (fr)	2017-07-11	2019-01-17	Syngenta Participations Ag	Dérivés d'oxadiazole microbiocides
WO2019011928A1 (fr)	2017-07-11	2019-01-17	Syngenta Participations Ag	Dérivés d'oxadiazole microbiocides
BR112020000465B1 (pt)	2017-07-11	2024-02-20	Syngenta Participations Ag	Derivados oxadiazol microbiocidas
WO2019011926A1 (fr)	2017-07-11	2019-01-17	Syngenta Participations Ag	Dérivés d'oxadiazole microbiocides
WO2019012011A1 (fr)	2017-07-12	2019-01-17	Syngenta Participations Ag	Dérivés d'oxadiazole microbiocides
BR112020000414A2 (pt)	2017-07-12	2020-07-21	Syngenta Participations Ag	derivados de oxadiazol microbicidas
WO2019012003A1 (fr)	2017-07-13	2019-01-17	Syngenta Participations Ag	Dérivés d'oxadiazole microbiocides
UY37912A (es)	2017-10-05	2019-05-31	Syngenta Participations Ag	Derivados de picolinamida fungicidas que portan grupos terminales heteroarilo o heteroariloxi
UY37913A (es)	2017-10-05	2019-05-31	Syngenta Participations Ag	Derivados de picolinamida fungicidas que portan un grupo terminal cuaternario
BR112020009659A2 (pt)	2017-11-15	2020-11-10	Syngenta Participations Ag	derivados picolinamida microbiocidas
CN112020503A (zh)	2018-04-26	2020-12-01	先正达参股股份有限公司	杀微生物的噁二唑衍生物
WO2020002331A1 (fr)	2018-06-29	2020-01-02	Syngenta Crop Protection Ag	Dérivés d'oxadiazole microbiocides
US20210284633A1 (en)	2018-07-02	2021-09-16	Syngenta Crop Protection Ag	3-(2-thienyl)-5-(trifluoromethyl)-1,2,4-oxadiazole derivatives as agrochemical fungicides
EP3823966A1 (fr)	2018-07-16	2021-05-26	Syngenta Crop Protection AG	Dérivés d'oxadiazole microbiocides
CN113195462A (zh)	2018-10-17	2021-07-30	先正达农作物保护股份公司	杀微生物的噁二唑衍生物
AR116628A1 (es)	2018-10-18	2021-05-26	Syngenta Crop Protection Ag	Compuestos microbiocidas
WO2020165403A1 (fr)	2019-02-15	2020-08-20	Syngenta Crop Protection Ag	Dérivés de thiazole substitués par phényle en tant que composés microbiocides
EA202192238A1 (ru)	2019-02-20	2022-01-20	Сингента Кроп Протекшн Аг	Применение спиропидиона
GB201903942D0 (en)	2019-03-22	2019-05-08	Syngenta Crop Protection Ag	Microbiocidal compounds
WO2020208095A1 (fr)	2019-04-10	2020-10-15	Syngenta Crop Protection Ag	Dérivés de picolinamide microbiocides
WO2020208096A1 (fr)	2019-04-10	2020-10-15	Syngenta Crop Protection Ag	Compositions fongicides
US20220264877A1 (en)	2019-07-05	2022-08-25	Syngenta Crop Protection Ag	Microbiocidal picolinamide derivatives
GB201910037D0 (en)	2019-07-12	2019-08-28	Syngenta Crop Protection Ag	Microbiocidal compounds
UY39115A (es)	2020-03-05	2021-10-29	Syngenta Crop Protection Ag	Mezclas fungicidas de derivados de arilo metoxiacrilato
UY39114A (es)	2020-03-05	2021-10-29	Syngenta Crop Protection Ag	Mezclas fungicidas de derivados de arilo metoxiacrilato
GB202006386D0 (en)	2020-04-30	2020-06-17	Syngenta Crop Protection Ag	Microbiocidal Compounds
GB202006399D0 (en)	2020-04-30	2020-06-17	Syngenta Crop Protection Ag	Microbiocidal compounds
GB202006480D0 (en)	2020-05-01	2020-06-17	Syngenta Crop Protection Ag	Microbiocidal compounds
GB202006606D0 (en)	2020-05-05	2020-06-17	Syngenta Crop Protection Ag	Microbiocidal compounds
GB202014840D0 (en)	2020-09-21	2020-11-04	Syngenta Crop Protection Ag	Microbiocidal compounds
KR102383881B1 (ko) *	2020-10-16	2022-04-06	국립생태원	유전자변형 옥수수의 동시 검출 방법
TW202231187A (zh)	2020-11-27	2022-08-16	瑞士商先正達農作物保護公司	殺有害生物組成物
PY21103556A (es)	2020-12-02	2023-05-03	Syngenta Crop Protection Ag	Composiciones fungicidas
WO2022117650A1 (fr)	2020-12-02	2022-06-09	Syngenta Crop Protection Ag	Compositions fongicides
PY2219081A (es)	2021-03-19	2022-09-27	Syngenta Crop Protection Ag	Composiciones plaguicidas
ES3052085T3 (en)	2021-05-04	2025-12-30	Syngenta Crop Protection Ag	Use of clethodim for insect control
WO2023275116A1 (fr)	2021-07-02	2023-01-05	Syngenta Crop Protection Ag	Utilisation de fluazifop-p-butyle pour lutter contre des insectes

Citations (4)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
WO2005059103A2 (fr) *	2003-12-15	2005-06-30	Monsanto Technology Llc	Plant de mais mon88017, compositions et procedes de detection associes
WO2005107437A2 (fr) *	2004-04-30	2005-11-17	Dow Agrosciences Llc	Nouveaux genes de resistance aux herbicides
RU2270254C2 (ru) *	2004-04-30	2006-02-20	Институт Молекулярной Биологии Им. В.А. Энгельгардта Российской Академии Наук	Способ идентификации трансгенных последовательностей днк в растительном материале и продуктах на его основе, набор олигонуклеотидов и биочип для осуществления этого способа
WO2008143993A2 (fr) *	2007-05-17	2008-11-27	Monsanto Technology Llc	Polymorphismes de maïs et procédés de génotypage

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US6245974B1 (en) *	1997-08-06	2001-06-12	North Carolina State University	Matrix attachment regions
CL2003002461A1 (es) *	2002-11-27	2005-01-07	Dow Chemical Company Agroscien	Inmunoglobulina que comprende al menos un glicano afucosilado, composicion que la contiene, secuencia nucleotidica y vector que la comprende, procedimiento para producir dicha inmunoglobulina en plantas.
MX338249B (es) *	2009-08-19	2016-04-08	Dow Agrosciences Llc	Evento das-40278-9 de ariloaxialcanoato dioxigenasa-1 lineas transgenicas de maiz relacionadas e identificacion de evento-especifico de las mismas.
US8598413B2 (en) *	2009-08-19	2013-12-03	Dow AgroSciecnes, LLC.	AAD-1 event DAS-40278-9, related transgenic corn lines, event-specific identification thereof, and methods of weed control involving AAD-1

2010
- 2010-08-18 CN CN201080047115.6A patent/CN102575299B/zh active Active
- 2010-08-18 JP JP2012525662A patent/JP5771208B2/ja not_active Expired - Fee Related
- 2010-08-18 NZ NZ598598A patent/NZ598598A/en not_active IP Right Cessation
- 2010-08-18 MX MX2012002076A patent/MX336072B/es unknown
- 2010-08-18 WO PCT/US2010/045871 patent/WO2011022471A1/fr not_active Ceased
- 2010-08-18 AU AU2010284286A patent/AU2010284286B2/en not_active Ceased
- 2010-08-18 CA CA2771581A patent/CA2771581C/fr active Active
- 2010-08-18 IN IN1800DEN2012 patent/IN2012DN01800A/en unknown
- 2010-08-18 KR KR1020127006871A patent/KR101772638B1/ko not_active Expired - Fee Related
- 2010-08-18 RU RU2012110253/10A patent/RU2577143C2/ru not_active IP Right Cessation
2012
- 2012-02-16 IL IL218175A patent/IL218175A/en not_active IP Right Cessation
- 2012-02-16 CL CL2012000410A patent/CL2012000410A1/es unknown
- 2012-02-17 ZA ZA2012/01210A patent/ZA201201210B/en unknown
- 2012-03-06 CO CO12039002A patent/CO6511214A2/es not_active Application Discontinuation

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
WO2005059103A2 (fr) *	2003-12-15	2005-06-30	Monsanto Technology Llc	Plant de mais mon88017, compositions et procedes de detection associes
WO2005107437A2 (fr) *	2004-04-30	2005-11-17	Dow Agrosciences Llc	Nouveaux genes de resistance aux herbicides
RU2270254C2 (ru) *	2004-04-30	2006-02-20	Институт Молекулярной Биологии Им. В.А. Энгельгардта Российской Академии Наук	Способ идентификации трансгенных последовательностей днк в растительном материале и продуктах на его основе, набор олигонуклеотидов и биочип для осуществления этого способа
WO2008143993A2 (fr) *	2007-05-17	2008-11-27	Monsanto Technology Llc	Polymorphismes de maïs et procédés de génotypage

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
GERMAN M.A.et al., A rapid method for the analysis of zygosity in transgenic plants, Plant Science, 2003, Vol. 164, pp. 183-187. *

Also Published As

Publication number	Publication date
HK1173194A1 (zh)	2013-05-10
CO6511214A2 (es)	2012-08-31
CA2771581C (fr)	2018-05-01
CL2012000410A1 (es)	2012-10-19
CN102575299B (zh)	2015-09-09
WO2011022471A1 (fr)	2011-02-24
CA2771581A1 (fr)	2011-02-24
JP2013505706A (ja)	2013-02-21
RU2012110253A (ru)	2013-09-27
NZ598598A (en)	2014-05-30
IL218175A0 (en)	2012-07-31
IL218175A (en)	2017-04-30
MX2012002076A (es)	2012-03-21
MX336072B (es)	2016-01-06
JP5771208B2 (ja)	2015-08-26
KR101772638B1 (ko)	2017-08-31
CN102575299A (zh)	2012-07-11
ZA201201210B (en)	2012-10-31
KR20120053038A (ko)	2012-05-24
AU2010284286A1 (en)	2012-03-29
BR112012003873A2 (pt)	2016-11-22
AU2010284286B2 (en)	2015-08-20
IN2012DN01800A (fr)	2015-06-05

Legal Events

Date	Code	Title	Description
2019-07-12	MM4A	The patent is invalid due to non-payment of fees	Effective date: 20180819

Publication	Publication Date	Title
RU2577143C2 (ru)	2016-03-10	Детекция aad-1 объекта das-40278-9
US9353381B2 (en)	2016-05-31	Cotton event pDAB4468.19.10.3 detection method
WO2011066360A1 (fr)	2011-06-03	Détection de l'événement 416 du soja aad-12
RU2573898C2 (ru)	2016-01-27	Детектирование aad-12-события 416 у сои
US20110151441A1 (en)	2011-06-23	Endpoint taqman methods for determining zygosity of corn comprising tc1507 events
US9204599B2 (en)	2015-12-08	Detection of AAD1 event DAS-40278-9
AU2011311955B9 (en)	2015-08-06	Endpoint TaqMan methods for determining zygosity of cotton comprising Cry 1F event 281-24-236
US9534260B2 (en)	2017-01-03	Materials and methods for detecting the aryloxyalkanoate dioxygenase gene (AAD-12) containing event pDAB4472-1606 in plants
US9243287B2 (en)	2016-01-26	Endpoint TaqMan methods for determining zygosity of cotton comprising Cry1Ac event 3006-210-23
EP2467500B1 (fr)	2016-06-01	Détection d'événement aad-1 das-40278-9
EP2768981B1 (fr)	2018-02-14	Procédé permettant de déterminer la zygosité du gène fad2 dans le colza canola à l'aide d'une pcr en point final
HK1173194B (en)	2016-09-02	Detection of aad-1 event das-40278-9
BR112012003873B1 (pt)	2025-01-28	Método para determinar zigosidade de uma planta de milho compreendendo um transgene de ariloxialcanoato dioxigenase (aad-1) como encontrado no evento de milho 5 das-40278-9, e kit para realizar o referido método