SE441363B - Metod for tverbindning av agarprodukter i alkalisk miljo - Google Patents
Metod for tverbindning av agarprodukter i alkalisk miljoInfo
- Publication number
- SE441363B SE441363B SE8400305A SE8400305A SE441363B SE 441363 B SE441363 B SE 441363B SE 8400305 A SE8400305 A SE 8400305A SE 8400305 A SE8400305 A SE 8400305A SE 441363 B SE441363 B SE 441363B
- Authority
- SE
- Sweden
- Prior art keywords
- crosslinking
- carried out
- sodium borohydride
- agarose
- flow rate
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims description 11
- 229920001817 Agar Polymers 0.000 title claims description 5
- 239000008272 agar Substances 0.000 title claims description 5
- 238000004132 cross linking Methods 0.000 claims description 26
- 239000012279 sodium borohydride Substances 0.000 claims description 13
- 229910000033 sodium borohydride Inorganic materials 0.000 claims description 13
- 229920002307 Dextran Polymers 0.000 claims description 9
- 239000000126 substance Substances 0.000 claims description 9
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 claims description 6
- 125000002887 hydroxy group Chemical group [H]O* 0.000 claims description 6
- 235000010355 mannitol Nutrition 0.000 claims description 5
- 125000000391 vinyl group Chemical group [H]C([*])=C([H])[H] 0.000 claims description 5
- 229930195725 Mannitol Natural products 0.000 claims description 4
- 239000003638 chemical reducing agent Substances 0.000 claims description 4
- 229930182830 galactose Natural products 0.000 claims description 4
- 239000000594 mannitol Substances 0.000 claims description 4
- WQZGKKKJIJFFOK-PHYPRBDBSA-N alpha-D-galactose Chemical compound OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-PHYPRBDBSA-N 0.000 claims description 3
- 229920000856 Amylose Polymers 0.000 claims description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 2
- 229920002907 Guar gum Polymers 0.000 claims description 2
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 claims description 2
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 2
- 230000009849 deactivation Effects 0.000 claims description 2
- AFOSIXZFDONLBT-UHFFFAOYSA-N divinyl sulfone Chemical compound C=CS(=O)(=O)C=C AFOSIXZFDONLBT-UHFFFAOYSA-N 0.000 claims description 2
- 239000008103 glucose Substances 0.000 claims description 2
- 235000010417 guar gum Nutrition 0.000 claims description 2
- FZWBNHMXJMCXLU-BLAUPYHCSA-N isomaltotriose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@@H](OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O)O1 FZWBNHMXJMCXLU-BLAUPYHCSA-N 0.000 claims description 2
- 230000007935 neutral effect Effects 0.000 claims description 2
- 229920000642 polymer Polymers 0.000 claims description 2
- 239000004971 Cross linker Substances 0.000 claims 1
- 229920000936 Agarose Polymers 0.000 description 12
- 238000001179 sorption measurement Methods 0.000 description 8
- 239000011324 bead Substances 0.000 description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
- 239000000499 gel Substances 0.000 description 6
- 108090000623 proteins and genes Proteins 0.000 description 5
- 102000004169 proteins and genes Human genes 0.000 description 5
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 4
- 238000003756 stirring Methods 0.000 description 4
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 238000004587 chromatography analysis Methods 0.000 description 3
- 230000003247 decreasing effect Effects 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- 102000001554 Hemoglobins Human genes 0.000 description 2
- 108010054147 Hemoglobins Proteins 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000001257 hydrogen Substances 0.000 description 2
- 229910052739 hydrogen Inorganic materials 0.000 description 2
- 229910000029 sodium carbonate Inorganic materials 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 101100283604 Caenorhabditis elegans pigk-1 gene Proteins 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- VVQNEPGJFQJSBK-UHFFFAOYSA-N Methyl methacrylate Chemical compound COC(=O)C(C)=C VVQNEPGJFQJSBK-UHFFFAOYSA-N 0.000 description 1
- 229920005372 Plexiglas® Polymers 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- DNJIEGIFACGWOD-UHFFFAOYSA-N ethyl mercaptane Natural products CCS DNJIEGIFACGWOD-UHFFFAOYSA-N 0.000 description 1
- 239000012847 fine chemical Substances 0.000 description 1
- 150000002431 hydrogen Chemical class 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- -1 may be used Substances 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 239000012429 reaction media Substances 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 239000001488 sodium phosphate Substances 0.000 description 1
- 229910000162 sodium phosphate Inorganic materials 0.000 description 1
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0036—Galactans; Derivatives thereof
- C08B37/0039—Agar; Agarose, i.e. D-galactose, 3,6-anhydro-D-galactose, methylated, sulfated, e.g. from the red algae Gelidium and Gracilaria; Agaropectin; Derivatives thereof, e.g. Sepharose, i.e. crosslinked agarose
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Materials Engineering (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Graft Or Block Polymers (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
Priority Applications (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE8400305A SE441363B (sv) | 1984-01-23 | 1984-01-23 | Metod for tverbindning av agarprodukter i alkalisk miljo |
| DE8585850011T DE3562253D1 (en) | 1984-01-23 | 1985-01-09 | Method for cross-linking of agar products |
| EP85850011A EP0153910B1 (fr) | 1984-01-23 | 1985-01-09 | Procédé pour produire des produits réticulés d'agar |
| JP60008303A JPS60179401A (ja) | 1984-01-23 | 1985-01-19 | 寒天生成物の架橋方法 |
| US06/693,106 US4591640A (en) | 1984-01-23 | 1985-01-22 | Method for cross-linking of agar products |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE8400305A SE441363B (sv) | 1984-01-23 | 1984-01-23 | Metod for tverbindning av agarprodukter i alkalisk miljo |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| SE8400305D0 SE8400305D0 (sv) | 1984-01-23 |
| SE8400305L SE8400305L (sv) | 1985-07-24 |
| SE441363B true SE441363B (sv) | 1985-09-30 |
Family
ID=20354398
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SE8400305A SE441363B (sv) | 1984-01-23 | 1984-01-23 | Metod for tverbindning av agarprodukter i alkalisk miljo |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US4591640A (fr) |
| EP (1) | EP0153910B1 (fr) |
| JP (1) | JPS60179401A (fr) |
| DE (1) | DE3562253D1 (fr) |
| SE (1) | SE441363B (fr) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CS249368B1 (en) * | 1984-12-29 | 1987-03-12 | Jan Peska | Method of pearls detran materials production for gel chromatography |
| JP2620039B2 (ja) * | 1991-12-20 | 1997-06-11 | アライド−シグナル・インコーポレーテッド | 天然高分子材料の多孔性架橋体 |
| US7906183B2 (en) * | 2007-03-30 | 2011-03-15 | Helena Laboratories Corporation | Process for manufacturing a flexible support for an electrophoretic medium |
| RU2665442C2 (ru) * | 2013-10-10 | 2018-08-29 | ДжиИ Хелткер Биопроцесс АрЭндДи АБ | Способ получения хроматографического материала |
| KR20170141245A (ko) | 2015-08-17 | 2017-12-22 | 이엠디 밀리포어 코포레이션 | 사이즈 기반 분리를 위한 아가로스 초여과 멤브레인 복합체 |
| CN108452775B (zh) * | 2018-04-02 | 2020-09-29 | 大连理工大学 | 一种功能化的高密度层析基质、其制备方法及应用 |
| EP4720085A1 (fr) | 2023-05-30 | 2026-04-08 | Mabxience Research, S.L. | Procede de purification d'immunoglobulines |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3959251A (en) * | 1970-06-25 | 1976-05-25 | Exploaterings Aktiebolaget T.B.F. | Stabilized agar product and method for its stabilization |
| SE403116B (sv) * | 1970-06-25 | 1978-07-31 | Exploaterings Ab Tbf | Stabiliserad agarprodukt samt sett for dess stabilisering |
-
1984
- 1984-01-23 SE SE8400305A patent/SE441363B/sv not_active IP Right Cessation
-
1985
- 1985-01-09 EP EP85850011A patent/EP0153910B1/fr not_active Expired
- 1985-01-09 DE DE8585850011T patent/DE3562253D1/de not_active Expired
- 1985-01-19 JP JP60008303A patent/JPS60179401A/ja active Granted
- 1985-01-22 US US06/693,106 patent/US4591640A/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| US4591640A (en) | 1986-05-27 |
| JPS60179401A (ja) | 1985-09-13 |
| DE3562253D1 (en) | 1988-05-26 |
| SE8400305L (sv) | 1985-07-24 |
| JPH0586802B2 (fr) | 1993-12-14 |
| SE8400305D0 (sv) | 1984-01-23 |
| EP0153910A1 (fr) | 1985-09-04 |
| EP0153910B1 (fr) | 1988-04-20 |
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