SE449003B - Sett att in vitro avskilja spermier med egen rorelseformaga medelst penetrationsmedium i vilket ingar hyaluronsyrasalt - Google Patents

Sett att in vitro avskilja spermier med egen rorelseformaga medelst penetrationsmedium i vilket ingar hyaluronsyrasalt

Info

Publication number: SE449003B
Authority: SE; Sweden
Prior art keywords: sperm; medium; penetration; layer; hyaluronic acid
Prior art date: 1985-10-18

Application number

SE8504872A

Other languages

English (en)

Swedish (sv)

Other versions

SE8504872D0 (sv

Inventor

Per Bergman

Bjoern Gustav Adolf Ingelman

Yvonne Steen

Original Assignee

Pharmacia Ab

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1985-10-18

Filing date

1985-10-18

Publication date

1987-03-30

1985-10-18 Application filed by Pharmacia Ab filed Critical Pharmacia Ab

1985-10-18 Priority to SE8504872A priority Critical patent/SE449003B/sv

1985-10-18 Publication of SE8504872D0 publication Critical patent/SE8504872D0/xx

1986-10-17 Priority to JP61505485A priority patent/JPS63501056A/ja

1986-10-17 Priority to DE8686906029T priority patent/DE3684462D1/de

1986-10-17 Priority to PCT/SE1986/000484 priority patent/WO1987002382A1/en

1986-10-17 Priority to EP86906029A priority patent/EP0243397B1/de

1986-10-17 Priority to US07/060,360 priority patent/US4804537A/en

1986-10-17 Priority to AT86906029T priority patent/ATE73843T1/de

1986-10-17 Priority to AU64751/86A priority patent/AU589702B2/en

1987-03-30 Publication of SE449003B publication Critical patent/SE449003B/sv

Links

230000035515 penetration Effects 0.000 title claims abstract description 53
KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical class CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 title claims abstract description 13
238000000338 in vitro Methods 0.000 title claims abstract description 10
238000000034 method Methods 0.000 claims abstract description 22
230000004720 fertilization Effects 0.000 claims abstract description 13
229920002674 hyaluronan Polymers 0.000 claims abstract description 12
229960003160 hyaluronic acid Drugs 0.000 claims abstract description 12
150000003839 salts Chemical class 0.000 claims description 15
230000004899 motility Effects 0.000 claims description 11
101100366942 Mus musculus Ston1 gene Proteins 0.000 abstract 1
239000000126 substance Substances 0.000 description 12
WCDDVEOXEIYWFB-VXORFPGASA-N (2s,3s,4r,5r,6r)-3-[(2s,3r,5s,6r)-3-acetamido-5-hydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-4,5,6-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@@H]1C[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](C(O)=O)O[C@@H](O)[C@H](O)[C@H]1O WCDDVEOXEIYWFB-VXORFPGASA-N 0.000 description 8
229940014041 hyaluronate Drugs 0.000 description 8
239000000243 solution Substances 0.000 description 7
150000002500 ions Chemical class 0.000 description 6
210000002966 serum Anatomy 0.000 description 6
102000009027 Albumins Human genes 0.000 description 5
108010088751 Albumins Proteins 0.000 description 5
239000000654 additive Substances 0.000 description 5
239000007788 liquid Substances 0.000 description 5
238000012360 testing method Methods 0.000 description 5
229920002385 Sodium hyaluronate Polymers 0.000 description 4
229940010747 sodium hyaluronate Drugs 0.000 description 4
YWIVKILSMZOHHF-QJZPQSOGSA-N sodium;(2s,3s,4s,5r,6r)-6-[(2s,3r,4r,5s,6r)-3-acetamido-2-[(2s,3s,4r,5r,6r)-6-[(2r,3r,4r,5s,6r)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2- Chemical compound [Na+].CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 YWIVKILSMZOHHF-QJZPQSOGSA-N 0.000 description 4
239000000872 buffer Substances 0.000 description 3
238000005119 centrifugation Methods 0.000 description 3
230000002950 deficient Effects 0.000 description 3
230000002349 favourable effect Effects 0.000 description 3
230000035558 fertility Effects 0.000 description 3
238000013508 migration Methods 0.000 description 3
230000005012 migration Effects 0.000 description 3
210000002381 plasma Anatomy 0.000 description 3
230000028327 secretion Effects 0.000 description 3
159000000000 sodium salts Chemical class 0.000 description 3
DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 2
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238000002474 experimental method Methods 0.000 description 2
150000004676 glycans Chemical class 0.000 description 2
230000036512 infertility Effects 0.000 description 2
244000005700 microbiome Species 0.000 description 2
235000015097 nutrients Nutrition 0.000 description 2
230000003204 osmotic effect Effects 0.000 description 2
229920001282 polysaccharide Polymers 0.000 description 2
239000005017 polysaccharide Substances 0.000 description 2
229910001414 potassium ion Inorganic materials 0.000 description 2
238000002360 preparation method Methods 0.000 description 2
239000000047 product Substances 0.000 description 2
230000001105 regulatory effect Effects 0.000 description 2
210000000582 semen Anatomy 0.000 description 2
239000011734 sodium Substances 0.000 description 2
229910001415 sodium ion Inorganic materials 0.000 description 2
239000006228 supernatant Substances 0.000 description 2
101100378191 Caenorhabditis elegans aco-2 gene Proteins 0.000 description 1
OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
241000282461 Canis lupus Species 0.000 description 1
102000004190 Enzymes Human genes 0.000 description 1
108090000790 Enzymes Proteins 0.000 description 1
229930091371 Fructose Natural products 0.000 description 1
RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
239000005715 Fructose Substances 0.000 description 1
241000287828 Gallus gallus Species 0.000 description 1
WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
102000008100 Human Serum Albumin Human genes 0.000 description 1
108091006905 Human Serum Albumin Proteins 0.000 description 1
ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
OSWRVYBYIGOAEZ-UHFFFAOYSA-N acetic acid;2-hydroxypropanoic acid Chemical compound CC(O)=O.CC(O)C(O)=O OSWRVYBYIGOAEZ-UHFFFAOYSA-N 0.000 description 1
230000003213 activating effect Effects 0.000 description 1
150000001412 amines Chemical class 0.000 description 1
239000003242 anti bacterial agent Substances 0.000 description 1
229940088710 antibiotic agent Drugs 0.000 description 1
239000007864 aqueous solution Substances 0.000 description 1
WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
239000007853 buffer solution Substances 0.000 description 1
239000011575 calcium Substances 0.000 description 1
229910052791 calcium Inorganic materials 0.000 description 1
125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
210000004027 cell Anatomy 0.000 description 1
150000003841 chloride salts Chemical class 0.000 description 1
210000001520 comb Anatomy 0.000 description 1
238000012258 culturing Methods 0.000 description 1
238000005516 engineering process Methods 0.000 description 1
238000011067 equilibration Methods 0.000 description 1
238000005194 fractionation Methods 0.000 description 1
230000004927 fusion Effects 0.000 description 1
239000008103 glucose Substances 0.000 description 1
238000001727 in vivo Methods 0.000 description 1
238000010348 incorporation Methods 0.000 description 1
208000015181 infectious disease Diseases 0.000 description 1
230000033001 locomotion Effects 0.000 description 1
229920002521 macromolecule Polymers 0.000 description 1
238000004519 manufacturing process Methods 0.000 description 1
239000000463 material Substances 0.000 description 1
239000000203 mixture Substances 0.000 description 1
230000027758 ovulation cycle Effects 0.000 description 1
239000008363 phosphate buffer Substances 0.000 description 1
239000002504 physiological saline solution Substances 0.000 description 1
239000011591 potassium Substances 0.000 description 1
229910052700 potassium Inorganic materials 0.000 description 1
102000004169 proteins and genes Human genes 0.000 description 1
108090000623 proteins and genes Proteins 0.000 description 1
238000004451 qualitative analysis Methods 0.000 description 1
238000004445 quantitative analysis Methods 0.000 description 1
229910052708 sodium Inorganic materials 0.000 description 1
239000012064 sodium phosphate buffer Substances 0.000 description 1
239000007787 solid Substances 0.000 description 1
230000019100 sperm motility Effects 0.000 description 1
230000009469 supplementation Effects 0.000 description 1
230000004083 survival effect Effects 0.000 description 1
239000000725 suspension Substances 0.000 description 1
239000011573 trace mineral Substances 0.000 description 1
235000013619 trace mineral Nutrition 0.000 description 1
238000005406 washing Methods 0.000 description 1
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0608—Germ cells
- C12N5/0612—Germ cells sorting of gametes, e.g. according to sex or motility
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/48—Reproductive organs
- A61K35/52—Sperm; Prostate; Seminal fluid; Leydig cells of testes

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Engineering & Computer Science (AREA)
Biomedical Technology (AREA)
Zoology (AREA)
Cell Biology (AREA)
Biotechnology (AREA)
Chemical & Material Sciences (AREA)
Reproductive Health (AREA)
Wood Science & Technology (AREA)
Organic Chemistry (AREA)
Developmental Biology & Embryology (AREA)
Genetics & Genomics (AREA)
Bioinformatics & Cheminformatics (AREA)
General Health & Medical Sciences (AREA)
Epidemiology (AREA)
Virology (AREA)
Public Health (AREA)
Veterinary Medicine (AREA)
Pharmacology & Pharmacy (AREA)
Medicinal Chemistry (AREA)
Microbiology (AREA)
Animal Behavior & Ethology (AREA)
Biochemistry (AREA)
General Engineering & Computer Science (AREA)
Immunology (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Surgical Instruments (AREA)
Media Introduction/Drainage Providing Device (AREA)
Agricultural Chemicals And Associated Chemicals (AREA)
Investigating Or Analysing Biological Materials (AREA)

SE8504872A 1985-10-18 1985-10-18 Sett att in vitro avskilja spermier med egen rorelseformaga medelst penetrationsmedium i vilket ingar hyaluronsyrasalt SE449003B (sv)

Priority Applications (8)

Application Number	Priority Date	Filing Date	Title
SE8504872A SE449003B (sv)	1985-10-18	1985-10-18	Sett att in vitro avskilja spermier med egen rorelseformaga medelst penetrationsmedium i vilket ingar hyaluronsyrasalt
JP61505485A JPS63501056A (ja)	1985-10-18	1986-10-17	運動性精子の選択方法および手段
DE8686906029T DE3684462D1 (de)	1985-10-18	1986-10-17	Trennverfahren fuer motile saatzellen in vitro, befruchtungsverfahren und durchdringungsmedium fuer saatzellen.
PCT/SE1986/000484 WO1987002382A1 (en)	1985-10-18	1986-10-17	Process for separating motile sperm cells in vitro, fertilization method and penetration medium for sperm cells
EP86906029A EP0243397B1 (de)	1985-10-18	1986-10-17	Trennverfahren für motile saatzellen in vitro, befruchtungsverfahren und durchdringungsmedium für saatzellen
US07/060,360 US4804537A (en)	1985-10-18	1986-10-17	Sperm selection process using a salt of hyaluronic acid
AT86906029T ATE73843T1 (de)	1985-10-18	1986-10-17	Trennverfahren fuer motile saatzellen in vitro, befruchtungsverfahren und durchdringungsmedium fuer saatzellen.
AU64751/86A AU589702B2 (en)	1985-10-18	1986-10-17	Process for separating motile sperm cells in vitro, fertilization method and penetration medium for sperm cells

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
SE8504872A SE449003B (sv)	1985-10-18	1985-10-18	Sett att in vitro avskilja spermier med egen rorelseformaga medelst penetrationsmedium i vilket ingar hyaluronsyrasalt

Publications (2)

Publication Number	Publication Date
SE8504872D0 SE8504872D0 (sv)	1985-10-18
SE449003B true SE449003B (sv)	1987-03-30

Family

ID=20361798

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
SE8504872A SE449003B (sv)	1985-10-18	1985-10-18	Sett att in vitro avskilja spermier med egen rorelseformaga medelst penetrationsmedium i vilket ingar hyaluronsyrasalt

Country Status (8)

Country	Link
US (1)	US4804537A (de)
EP (1)	EP0243397B1 (de)
JP (1)	JPS63501056A (de)
AT (1)	ATE73843T1 (de)
AU (1)	AU589702B2 (de)
DE (1)	DE3684462D1 (de)
SE (1)	SE449003B (de)
WO (1)	WO1987002382A1 (de)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
SE8901127D0 (sv) *	1989-03-31	1989-03-31	Aa H L Pousette	Reagenssats och dess anvaending foer att bilda (spap-anti-spap) -immunkomplex samt diagnostiskt foerfarand vid vilket spap detekteras
US5102783A (en) *	1990-01-12	1992-04-07	Vetrepharm, Inc.	Composition and method for culturing and freezing cells and tissues
EP0446509A1 (de) *	1990-03-15	1991-09-18	Panayiotis M. Zavos	Sperma-Säulenfilter
CA2061703C (en) *	1992-02-20	2002-07-02	Rudolf E. Falk	Formulations containing hyaluronic acid
US5910489A (en) *	1990-09-18	1999-06-08	Hyal Pharmaceutical Corporation	Topical composition containing hyaluronic acid and NSAIDS
US5990096A (en) *	1990-09-18	1999-11-23	Hyal Pharmaceutical Corporation	Formulations containing hyaluronic acid
US5824658A (en) *	1990-09-18	1998-10-20	Hyal Pharmaceutical Corporation	Topical composition containing hyaluronic acid and NSAIDS
US5977088A (en) *	1991-07-03	1999-11-02	Hyal Pharmaceutical Corporation	Formulations containing hyaluronic acid
US5792753A (en) *	1991-07-03	1998-08-11	Hyal Pharmaceutical Corporation	Compositions comprising hyaluronic acid and prostaglandin-synthesis-inhibiting drugs
US6103704A (en) *	1991-07-03	2000-08-15	Hyal Pharmaceutical Corporation	Therapeutic methods using hyaluronic acid
US6218373B1 (en)	1992-02-20	2001-04-17	Hyal Pharmaceutical Corporation	Formulations containing hyaluronic acid
US5767106A (en) *	1992-02-21	1998-06-16	Hyal Pharmaceutical Corporation	Treatment of disease and conditions associated with macrophage infiltration
ES2044778B1 (es) *	1992-04-02	1994-09-01	Esteve Carmen Blanco	Metodo analitico para la determinacion de la capacidad fecundante de una muestra de semen humano.
US5744366A (en) *	1992-05-01	1998-04-28	Trustees Of The University Of Pennsylvania	Mesoscale devices and methods for analysis of motile cells
US5801058A (en) *	1994-06-20	1998-09-01	Blanco-Esteve; Carmen	Analytic method to determine the fertilizing capacity of a human semen sample
US5935800A (en) *	1994-10-31	1999-08-10	Beth Israel Deaconess Medical Center	Assays and kits for determining male fertility
US5895749A (en) *	1994-10-31	1999-04-20	Beth Israel Deaconess Medical Center	Male fertility and contraception home test kits
WO1996013225A2 (en) *	1994-10-31	1996-05-09	Beth Israel Hospital Association	Assays, devices and kits for determining male fertility
AU723170B2 (en) *	1995-10-19	2000-08-17	Bio-Origyn Llc	Methods and compositions to improve germ cell and embryo survival and function
US6129214A (en) *	1997-12-19	2000-10-10	Bar-Ami; Shalom	Sperm strainer system
US5897988A (en) *	1998-01-21	1999-04-27	Yale University	Process and system for selection of mature sperm by surface membrane determinants for assisted reproduction
GB9817795D0 (en) *	1998-08-14	1998-10-14	Genosis Inc	A method and device for separation and detection of spermatozoa in a sample
WO2000032140A1 (en) *	1998-11-30	2000-06-08	Ivf Sciences Colorado, Inc.	System and sequential culture media for in vitro fertilization
US6762053B2 (en) *	2000-06-09	2004-07-13	Vitrolife, Inc.	Mammalian gamete and embryo culture media and culture media supplements
ES2259566B1 (es) *	2005-03-29	2007-08-01	Instituto Nacional De Investigacion Y Tecnologia Agraria Y Alimentaria-Inia	Suplementacion para los medios de manipulacion embrionaria y/o celular.
CN101487841B (zh) *	2009-02-13	2014-06-04	深圳市人民医院	一种包被载体及其在检测精子成熟度和无创分离成熟精子方法中的应用
ES2387903B1 (es) *	2010-07-16	2013-08-13	María Albiñana Blanco	Procedimiento y kit para verificar, en una muestra de semen humano o animal, la existencia de espermatozoides fecundantes.
CN102323398B (zh) *	2011-05-19	2014-03-12	国家人口计生委科学技术研究所	一种用于精子检测的溶液
RU2662080C1 (ru) *	2017-09-18	2018-07-23	Общество с ограниченной ответственностью "Генетико-селекционный исследовательский центр "Генология-МГУ"	Способ отбора генетически полноценных сперматозоидов сельскохозяйственных животных

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US4327177A (en) *	1969-04-10	1982-04-27	Wallace Shrimpton	Method and means for controlling the sex of mammalian offspring and product therefor
US4009260A (en) *	1973-04-19	1977-02-22	Schering Aktiengesellschaft	Fractionation of sperm
US4007087A (en) *	1975-10-17	1977-02-08	Gametrics Limited	Sperm fractionation and storage
US4339434A (en) *	1981-08-17	1982-07-13	Gametrics Limited	Method of increasing the incidence of female offspring

1985
- 1985-10-18 SE SE8504872A patent/SE449003B/sv not_active IP Right Cessation
1986
- 1986-10-17 WO PCT/SE1986/000484 patent/WO1987002382A1/en not_active Ceased
- 1986-10-17 US US07/060,360 patent/US4804537A/en not_active Expired - Lifetime
- 1986-10-17 AU AU64751/86A patent/AU589702B2/en not_active Expired
- 1986-10-17 AT AT86906029T patent/ATE73843T1/de not_active IP Right Cessation
- 1986-10-17 JP JP61505485A patent/JPS63501056A/ja active Granted
- 1986-10-17 DE DE8686906029T patent/DE3684462D1/de not_active Expired - Lifetime
- 1986-10-17 EP EP86906029A patent/EP0243397B1/de not_active Expired

Also Published As

Publication number	Publication date
AU589702B2 (en)	1989-10-19
JPH0317492B2 (de)	1991-03-08
EP0243397A1 (de)	1987-11-04
EP0243397B1 (de)	1992-03-18
AU6475186A (en)	1987-05-05
JPS63501056A (ja)	1988-04-21
WO1987002382A1 (en)	1987-04-23
US4804537A (en)	1989-02-14
ATE73843T1 (de)	1992-04-15
SE8504872D0 (sv)	1985-10-18
DE3684462D1 (de)	1992-04-23

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