SE537440C2 - Förfarande för enzymatisk hydrolysering av glukuronoxylan - Google Patents

Förfarande för enzymatisk hydrolysering av glukuronoxylan Download PDF

Info

Publication number: SE537440C2
Authority: SE; Sweden
Prior art keywords: xylan; polypeptide; seq; amino acid; homologous
Prior art date: 2009-03-31

Application number

SE1150989A

Other languages

English (en)

Swedish (sv)

Other versions

SE1150989A1 (sv

Inventor

Willem Heber Van Zyl

Peter Biely

Olena Ryabova

Annie Fabian Abel Chimphango

Johann Ferdinand Görgens

Original Assignee

Univ Stellenbosch

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2009-03-31

Filing date

2010-03-31

Publication date

2015-04-28

2010-03-31 Application filed by Univ Stellenbosch filed Critical Univ Stellenbosch

2011-12-21 Publication of SE1150989A1 publication Critical patent/SE1150989A1/sv

2015-04-28 Publication of SE537440C2 publication Critical patent/SE537440C2/sv

Links

238000000034 method Methods 0.000 title claims abstract description 50
230000008569 process Effects 0.000 title description 8
230000007071 enzymatic hydrolysis Effects 0.000 title description 3
238000006047 enzymatic hydrolysis reaction Methods 0.000 title description 3
108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 56
229920001184 polypeptide Polymers 0.000 claims abstract description 55
102000004196 processed proteins & peptides Human genes 0.000 claims abstract description 55
230000007062 hydrolysis Effects 0.000 claims abstract description 19
238000006460 hydrolysis reaction Methods 0.000 claims abstract description 19
-1 xylopyranosyl residue Chemical group 0.000 claims abstract description 19
241000235060 Scheffersomyces stipitis Species 0.000 claims abstract description 18
229920001706 Glucuronoxylan Polymers 0.000 claims abstract description 8
238000002360 preparation method Methods 0.000 claims abstract description 7
108091033319 polynucleotide Proteins 0.000 claims abstract description 5
102000040430 polynucleotide Human genes 0.000 claims abstract description 5
239000002157 polynucleotide Substances 0.000 claims abstract description 5
108090000790 Enzymes Proteins 0.000 claims description 60
102000004190 Enzymes Human genes 0.000 claims description 60
230000000694 effects Effects 0.000 claims description 41
230000006698 induction Effects 0.000 claims description 21
241000222481 Schizophyllum commune Species 0.000 claims description 14
238000005571 anion exchange chromatography Methods 0.000 claims description 14
241001633332 Scheffersomyces stipitis CBS 6054 Species 0.000 claims description 10
239000002773 nucleotide Substances 0.000 claims description 10
125000003729 nucleotide group Chemical group 0.000 claims description 10
241001225321 Aspergillus fumigatus Species 0.000 claims description 7
229940091771 aspergillus fumigatus Drugs 0.000 claims description 7
239000012634 fragment Substances 0.000 claims description 7
241001507865 Aspergillus fischeri Species 0.000 claims description 6
240000006439 Aspergillus oryzae Species 0.000 claims description 6
235000002247 Aspergillus oryzae Nutrition 0.000 claims description 6
241001465318 Aspergillus terreus Species 0.000 claims description 6
241001135228 Bacteroides ovatus Species 0.000 claims description 6
241000123365 Cryptococcus neoformans var. neoformans Species 0.000 claims description 6
241000223192 Fusarium sporotrichioides Species 0.000 claims description 6
241001541432 Opitutus terrae Species 0.000 claims description 6
241000736122 Parastagonospora nodorum Species 0.000 claims description 6
241001670248 Saccharophagus degradans Species 0.000 claims description 6
241001518258 Streptomyces pristinaespiralis Species 0.000 claims description 6
238000004587 chromatography analysis Methods 0.000 claims description 6
241000228193 Aspergillus clavatus Species 0.000 claims description 5
241000010977 Cellvibrio japonicus Species 0.000 claims description 5
235000001673 Coprinus macrorhizus Nutrition 0.000 claims description 5
241001344131 Magnaporthe grisea Species 0.000 claims description 5
241000123650 Botrytis cinerea Species 0.000 claims description 3
241000223195 Fusarium graminearum Species 0.000 claims description 3
241000190117 Pyrenophora tritici-repentis Species 0.000 claims description 3
238000012258 culturing Methods 0.000 claims description 2
230000002209 hydrophobic effect Effects 0.000 claims description 2
125000003275 alpha amino acid group Chemical group 0.000 claims 10
241000222512 Coprinopsis cinerea Species 0.000 claims 2
241000221961 Neurospora crassa Species 0.000 claims 2
241000221946 Podospora anserina Species 0.000 claims 2
241000221696 Sclerotinia sclerotiorum Species 0.000 claims 2
241000863387 Cellvibrio Species 0.000 claims 1
241000223218 Fusarium Species 0.000 claims 1
239000002028 Biomass Substances 0.000 abstract description 22
238000009629 microbiological culture Methods 0.000 abstract description 2
150000007523 nucleic acids Chemical group 0.000 abstract 1
125000002924 primary amino group Chemical group [H]N([H])* 0.000 abstract 1
150000004823 xylans Chemical group 0.000 description 146
229920001221 xylan Polymers 0.000 description 145
108010061261 alpha-glucuronidase Proteins 0.000 description 56
241000609240 Ambelania acida Species 0.000 description 41
239000010905 bagasse Substances 0.000 description 41
SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 description 40
SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 39
PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 38
238000001228 spectrum Methods 0.000 description 38
239000002253 acid Substances 0.000 description 34
235000017166 Bambusa arundinacea Nutrition 0.000 description 31
235000017491 Bambusa tulda Nutrition 0.000 description 31
241001330002 Bambuseae Species 0.000 description 31
235000015334 Phyllostachys viridis Nutrition 0.000 description 31
241001236214 Pinus patula Species 0.000 description 31
239000011425 bamboo Substances 0.000 description 31
238000004458 analytical method Methods 0.000 description 30
239000003480 eluent Substances 0.000 description 30
239000000203 mixture Substances 0.000 description 30
235000000346 sugar Nutrition 0.000 description 29
241001233195 Eucalyptus grandis Species 0.000 description 27
239000002609 medium Substances 0.000 description 27
238000000605 extraction Methods 0.000 description 25
108090000623 proteins and genes Proteins 0.000 description 25
235000018185 Betula X alpestris Nutrition 0.000 description 24
235000018212 Betula X uliginosa Nutrition 0.000 description 24
244000034068 Elaeocarpus grandis Species 0.000 description 22
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 21
235000018102 proteins Nutrition 0.000 description 21
102000004169 proteins and genes Human genes 0.000 description 21
229920005610 lignin Polymers 0.000 description 20
150000001413 amino acids Chemical group 0.000 description 19
239000000047 product Substances 0.000 description 19
239000000758 substrate Substances 0.000 description 19
229920002488 Hemicellulose Polymers 0.000 description 17
239000002994 raw material Substances 0.000 description 17
WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 16
IAJILQKETJEXLJ-UHFFFAOYSA-N Galacturonsaeure Natural products O=CC(O)C(O)C(O)C(O)C(O)=O IAJILQKETJEXLJ-UHFFFAOYSA-N 0.000 description 15
WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 15
229960001031 glucose Drugs 0.000 description 15
PYMYPHUHKUWMLA-WDCZJNDASA-N arabinose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)C=O PYMYPHUHKUWMLA-WDCZJNDASA-N 0.000 description 14
239000008103 glucose Substances 0.000 description 14
239000000126 substance Substances 0.000 description 14
OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 13
150000001720 carbohydrates Chemical class 0.000 description 13
235000014633 carbohydrates Nutrition 0.000 description 13
229910052799 carbon Inorganic materials 0.000 description 13
229920001282 polysaccharide Polymers 0.000 description 13
230000004044 response Effects 0.000 description 13
150000008163 sugars Chemical class 0.000 description 13
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 13
QGGOCWIJGWDKHC-FSIIMWSLSA-N (2s,3s,4r,5r)-2,4,5-trihydroxy-3-methoxy-6-oxohexanoic acid Chemical group OC(=O)[C@@H](O)[C@@H](OC)[C@H](O)[C@@H](O)C=O QGGOCWIJGWDKHC-FSIIMWSLSA-N 0.000 description 12
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 12
HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 12
229920002678 cellulose Polymers 0.000 description 12
239000001913 cellulose Substances 0.000 description 12
230000008021 deposition Effects 0.000 description 12
229940097043 glucuronic acid Drugs 0.000 description 12
HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 description 12
238000011160 research Methods 0.000 description 12
QGGOCWIJGWDKHC-UHFFFAOYSA-N O4-Methyl-D-glucuronsaeure Natural products OC(=O)C(O)C(OC)C(O)C(O)C=O QGGOCWIJGWDKHC-UHFFFAOYSA-N 0.000 description 11
239000000284 extract Substances 0.000 description 11
239000000463 material Substances 0.000 description 11
235000005106 Pinus patula Nutrition 0.000 description 10
IAJILQKETJEXLJ-QTBDOELSSA-N aldehydo-D-glucuronic acid Chemical compound O=C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)C(O)=O IAJILQKETJEXLJ-QTBDOELSSA-N 0.000 description 10
238000010828 elution Methods 0.000 description 10
230000014759 maintenance of location Effects 0.000 description 10
239000002023 wood Substances 0.000 description 10
238000001644 13C nuclear magnetic resonance spectroscopy Methods 0.000 description 9
238000005481 NMR spectroscopy Methods 0.000 description 9
240000004808 Saccharomyces cerevisiae Species 0.000 description 9
125000002777 acetyl group Chemical group [H]C([H])([H])C(*)=O 0.000 description 9
238000012512 characterization method Methods 0.000 description 9
238000010586 diagram Methods 0.000 description 9
239000007788 liquid Substances 0.000 description 9
238000000746 purification Methods 0.000 description 9
HNSDLXPSAYFUHK-UHFFFAOYSA-N 1,4-bis(2-ethylhexyl) sulfosuccinate Chemical compound CCCCC(CC)COC(=O)CC(S(O)(=O)=O)C(=O)OCC(CC)CCCC HNSDLXPSAYFUHK-UHFFFAOYSA-N 0.000 description 8
238000005160 1H NMR spectroscopy Methods 0.000 description 8
241000196324 Embryophyta Species 0.000 description 8
235000008331 Pinus X rigitaeda Nutrition 0.000 description 8
241000018646 Pinus brutia Species 0.000 description 8
235000011613 Pinus brutia Nutrition 0.000 description 8
238000006243 chemical reaction Methods 0.000 description 8
238000009472 formulation Methods 0.000 description 8
125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 8
238000009835 boiling Methods 0.000 description 7
150000001875 compounds Chemical class 0.000 description 7
239000012149 elution buffer Substances 0.000 description 7
230000006870 function Effects 0.000 description 7
229930182830 galactose Natural products 0.000 description 7
229930182470 glycoside Natural products 0.000 description 7
108010001817 Endo-1,4-beta Xylanases Proteins 0.000 description 6
OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
WQZGKKKJIJFFOK-PHYPRBDBSA-N alpha-D-galactose Chemical compound OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-PHYPRBDBSA-N 0.000 description 6
235000001014 amino acid Nutrition 0.000 description 6
229940024606 amino acid Drugs 0.000 description 6
239000000835 fiber Substances 0.000 description 6
230000003993 interaction Effects 0.000 description 6
238000000655 nuclear magnetic resonance spectrum Methods 0.000 description 6
150000004804 polysaccharides Chemical class 0.000 description 6
239000011780 sodium chloride Substances 0.000 description 6
239000000243 solution Substances 0.000 description 6
WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 description 5
241000219927 Eucalyptus Species 0.000 description 5
VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 5
TVXBFESIOXBWNM-UHFFFAOYSA-N Xylitol Natural products OCCC(O)C(O)C(O)CCO TVXBFESIOXBWNM-UHFFFAOYSA-N 0.000 description 5
239000008351 acetate buffer Substances 0.000 description 5
150000007513 acids Chemical class 0.000 description 5
125000000089 arabinosyl group Chemical group C1([C@@H](O)[C@H](O)[C@H](O)CO1)* 0.000 description 5
238000001514 detection method Methods 0.000 description 5
230000002255 enzymatic effect Effects 0.000 description 5
238000004191 hydrophobic interaction chromatography Methods 0.000 description 5
238000004519 manufacturing process Methods 0.000 description 5
239000011159 matrix material Substances 0.000 description 5
239000005017 polysaccharide Substances 0.000 description 5
238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 5
239000000811 xylitol Substances 0.000 description 5
HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 description 5
229960002675 xylitol Drugs 0.000 description 5
235000010447 xylitol Nutrition 0.000 description 5
125000000969 xylosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)CO1)* 0.000 description 5
FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 4
238000005033 Fourier transform infrared spectroscopy Methods 0.000 description 4
238000001157 Fourier transform infrared spectrum Methods 0.000 description 4
125000001429 N-terminal alpha-amino-acid group Chemical group 0.000 description 4
238000005903 acid hydrolysis reaction Methods 0.000 description 4
230000002378 acidificating effect Effects 0.000 description 4
AEMOLEFTQBMNLQ-WAXACMCWSA-N alpha-D-glucuronic acid Chemical compound O[C@H]1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]1O AEMOLEFTQBMNLQ-WAXACMCWSA-N 0.000 description 4
238000000540 analysis of variance Methods 0.000 description 4
239000003153 chemical reaction reagent Substances 0.000 description 4
230000000875 corresponding effect Effects 0.000 description 4
238000005384 cross polarization magic-angle spinning Methods 0.000 description 4
238000013461 design Methods 0.000 description 4
238000005194 fractionation Methods 0.000 description 4
239000000499 gel Substances 0.000 description 4
150000002338 glycosides Chemical class 0.000 description 4
229910052739 hydrogen Inorganic materials 0.000 description 4
230000009021 linear effect Effects 0.000 description 4
230000000813 microbial effect Effects 0.000 description 4
230000007935 neutral effect Effects 0.000 description 4
238000001556 precipitation Methods 0.000 description 4
239000000523 sample Substances 0.000 description 4
239000012064 sodium phosphate buffer Substances 0.000 description 4
239000007787 solid Substances 0.000 description 4
239000007790 solid phase Substances 0.000 description 4
238000004482 13C cross polarization magic angle spinning Methods 0.000 description 3
WGLLPAPKWFDHHV-UHFFFAOYSA-N 4-O-Methyl-L-idopyranuronsaeure Natural products COC1C(O)C(O)C(O)OC1C(O)=O WGLLPAPKWFDHHV-UHFFFAOYSA-N 0.000 description 3
229920003043 Cellulose fiber Polymers 0.000 description 3
244000251987 Coprinus macrorhizus Species 0.000 description 3
125000000333 D-xylopyranosyl group Chemical group [H]O[C@]1([H])C([H])([H])OC([H])(*)[C@]([H])(O[H])[C@@]1([H])O[H] 0.000 description 3
RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 3
240000000905 Nymphoides indica Species 0.000 description 3
235000017590 Nymphoides indica Nutrition 0.000 description 3
230000009471 action Effects 0.000 description 3
125000001931 aliphatic group Chemical group 0.000 description 3
239000003513 alkali Substances 0.000 description 3
125000003118 aryl group Chemical group 0.000 description 3
238000003556 assay Methods 0.000 description 3
125000000484 butyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 3
238000001460 carbon-13 nuclear magnetic resonance spectrum Methods 0.000 description 3
230000015556 catabolic process Effects 0.000 description 3
HEBKCHPVOIAQTA-NGQZWQHPSA-N d-xylitol Chemical compound OC[C@H](O)C(O)[C@H](O)CO HEBKCHPVOIAQTA-NGQZWQHPSA-N 0.000 description 3
230000003247 decreasing effect Effects 0.000 description 3
238000006731 degradation reaction Methods 0.000 description 3
238000005516 engineering process Methods 0.000 description 3
238000002474 experimental method Methods 0.000 description 3
235000013305 food Nutrition 0.000 description 3
239000001307 helium Substances 0.000 description 3
229910052734 helium Inorganic materials 0.000 description 3
SWQJXJOGLNCZEY-UHFFFAOYSA-N helium atom Chemical compound [He] SWQJXJOGLNCZEY-UHFFFAOYSA-N 0.000 description 3
238000011534 incubation Methods 0.000 description 3
239000012978 lignocellulosic material Substances 0.000 description 3
244000005700 microbiome Species 0.000 description 3
229920000642 polymer Polymers 0.000 description 3
238000006116 polymerization reaction Methods 0.000 description 3
BDERNNFJNOPAEC-UHFFFAOYSA-N propan-1-ol Chemical compound CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 description 3
230000000717 retained effect Effects 0.000 description 3
239000007974 sodium acetate buffer Substances 0.000 description 3
238000006467 substitution reaction Methods 0.000 description 3
238000010998 test method Methods 0.000 description 3
JNIQBPHJIAOMQU-FSIIMWSLSA-N (2s,3s,4s,5r)-2,3,4,5-tetrahydroxy-6-oxoheptanoic acid Chemical compound CC(=O)[C@H](O)[C@@H](O)[C@H](O)[C@H](O)C(O)=O JNIQBPHJIAOMQU-FSIIMWSLSA-N 0.000 description 2
KSEBMYQBYZTDHS-HWKANZROSA-M (E)-Ferulic acid Natural products COC1=CC(\C=C\C([O-])=O)=CC=C1O KSEBMYQBYZTDHS-HWKANZROSA-M 0.000 description 2
MYRTYDVEIRVNKP-UHFFFAOYSA-N 1,2-Divinylbenzene Chemical compound C=CC1=CC=CC=C1C=C MYRTYDVEIRVNKP-UHFFFAOYSA-N 0.000 description 2
241000228212 Aspergillus Species 0.000 description 2
241000218631 Coniferophyta Species 0.000 description 2
DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 2
241001471187 Patu Species 0.000 description 2
ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical group OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
241000221945 Podospora Species 0.000 description 2
240000000111 Saccharum officinarum Species 0.000 description 2
235000007201 Saccharum officinarum Nutrition 0.000 description 2
LEHOTFFKMJEONL-UHFFFAOYSA-N Uric Acid Chemical compound N1C(=O)NC(=O)C2=C1NC(=O)N2 LEHOTFFKMJEONL-UHFFFAOYSA-N 0.000 description 2
TVWHNULVHGKJHS-UHFFFAOYSA-N Uric acid Natural products N1C(=O)NC(=O)C2NC(=O)NC21 TVWHNULVHGKJHS-UHFFFAOYSA-N 0.000 description 2
MCMNRKCIXSYSNV-UHFFFAOYSA-N Zirconium dioxide Chemical compound O=[Zr]=O MCMNRKCIXSYSNV-UHFFFAOYSA-N 0.000 description 2
238000009825 accumulation Methods 0.000 description 2
YRKCREAYFQTBPV-UHFFFAOYSA-N acetylacetone Chemical compound CC(=O)CC(C)=O YRKCREAYFQTBPV-UHFFFAOYSA-N 0.000 description 2
108010093941 acetylxylan esterase Proteins 0.000 description 2
230000002776 aggregation Effects 0.000 description 2
238000004220 aggregation Methods 0.000 description 2
125000000328 arabinofuranosyl group Chemical group C1([C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 2
150000001491 aromatic compounds Chemical class 0.000 description 2
239000000872 buffer Substances 0.000 description 2
229940077731 carbohydrate nutrients Drugs 0.000 description 2
238000001875 carbon-13 cross-polarisation magic angle spinning nuclear magnetic resonance spectrum Methods 0.000 description 2
125000002915 carbonyl group Chemical group [*:2]C([*:1])=O 0.000 description 2
230000003197 catalytic effect Effects 0.000 description 2
239000011248 coating agent Substances 0.000 description 2
238000000576 coating method Methods 0.000 description 2
238000001160 cross-polarisation magic angle spinning nuclear magnetic resonance spectrum Methods 0.000 description 2
239000008367 deionised water Substances 0.000 description 2
229910021641 deionized water Inorganic materials 0.000 description 2
238000011161 development Methods 0.000 description 2
230000018109 developmental process Effects 0.000 description 2
229910003460 diamond Inorganic materials 0.000 description 2
239000010432 diamond Substances 0.000 description 2
230000008034 disappearance Effects 0.000 description 2
230000007515 enzymatic degradation Effects 0.000 description 2
238000006911 enzymatic reaction Methods 0.000 description 2
238000012869 ethanol precipitation Methods 0.000 description 2
229940114124 ferulic acid Drugs 0.000 description 2
KSEBMYQBYZTDHS-UHFFFAOYSA-N ferulic acid Natural products COC1=CC(C=CC(O)=O)=CC=C1O KSEBMYQBYZTDHS-UHFFFAOYSA-N 0.000 description 2
235000001785 ferulic acid Nutrition 0.000 description 2
238000001914 filtration Methods 0.000 description 2
239000011521 glass Substances 0.000 description 2
230000003301 hydrolyzing effect Effects 0.000 description 2
238000004255 ion exchange chromatography Methods 0.000 description 2
239000012528 membrane Substances 0.000 description 2
BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 2
239000000178 monomer Substances 0.000 description 2
229920001542 oligosaccharide Polymers 0.000 description 2
150000002482 oligosaccharides Chemical class 0.000 description 2
238000005457 optimization Methods 0.000 description 2
239000012071 phase Substances 0.000 description 2
230000037039 plant physiology Effects 0.000 description 2
229920000307 polymer substrate Polymers 0.000 description 2
239000011148 porous material Substances 0.000 description 2
238000004321 preservation Methods 0.000 description 2
238000000425 proton nuclear magnetic resonance spectrum Methods 0.000 description 2
239000002904 solvent Substances 0.000 description 2
238000004611 spectroscopical analysis Methods 0.000 description 2
238000007619 statistical method Methods 0.000 description 2
238000003860 storage Methods 0.000 description 2
238000012360 testing method Methods 0.000 description 2
QURCVMIEKCOAJU-UHFFFAOYSA-N trans-isoferulic acid Natural products COC1=CC=C(C=CC(O)=O)C=C1O QURCVMIEKCOAJU-UHFFFAOYSA-N 0.000 description 2
238000011282 treatment Methods 0.000 description 2
229940116269 uric acid Drugs 0.000 description 2
238000003828 vacuum filtration Methods 0.000 description 2
150000003741 xylose derivatives Chemical group 0.000 description 2
HGUFODBRKLSHSI-UHFFFAOYSA-N 2,3,7,8-tetrachloro-dibenzo-p-dioxin Chemical compound O1C2=CC(Cl)=C(Cl)C=C2OC2=C1C=C(Cl)C(Cl)=C2 HGUFODBRKLSHSI-UHFFFAOYSA-N 0.000 description 1
OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 description 1
101710114355 4-O-methyl-glucuronoyl methylesterase Proteins 0.000 description 1
FNEHAOQZWPHONV-UHFFFAOYSA-N 9h-carbazole;sulfuric acid Chemical compound OS(O)(=O)=O.C1=CC=C2C3=CC=CC=C3NC2=C1 FNEHAOQZWPHONV-UHFFFAOYSA-N 0.000 description 1
108010013043 Acetylesterase Proteins 0.000 description 1
HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 1
101710089396 Alpha-glucuronidase A Proteins 0.000 description 1
101001065065 Aspergillus awamori Feruloyl esterase A Proteins 0.000 description 1
241000962950 Bacteroides ovatus ATCC 8483 Species 0.000 description 1
241000855157 Bambusa balcooa Species 0.000 description 1
108091003079 Bovine Serum Albumin Proteins 0.000 description 1
244000025254 Cannabis sativa Species 0.000 description 1
241000646940 Cellvibrio japonicus Ueda107 Species 0.000 description 1
241000254173 Coleoptera Species 0.000 description 1
RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
241000960997 Coprinopsis cinerea okayama7#130 Species 0.000 description 1
235000018139 Coprinopsis cinerea okayama7130 Nutrition 0.000 description 1
241001275954 Cortinarius caperatus Species 0.000 description 1
241001362614 Crassa Species 0.000 description 1
XDTMQSROBMDMFD-UHFFFAOYSA-N Cyclohexane Chemical compound C1CCCCC1 XDTMQSROBMDMFD-UHFFFAOYSA-N 0.000 description 1
AEMOLEFTQBMNLQ-DTEWXJGMSA-N D-Galacturonic acid Natural products O[C@@H]1O[C@H](C(O)=O)[C@H](O)[C@H](O)[C@H]1O AEMOLEFTQBMNLQ-DTEWXJGMSA-N 0.000 description 1
101710121765 Endo-1,4-beta-xylanase Proteins 0.000 description 1
108090000371 Esterases Proteins 0.000 description 1
108050001049 Extracellular proteins Proteins 0.000 description 1
241000233866 Fungi Species 0.000 description 1
JVTAAEKCZFNVCJ-UHFFFAOYSA-M Lactate Chemical compound CC(O)C([O-])=O JVTAAEKCZFNVCJ-UHFFFAOYSA-M 0.000 description 1
238000005004 MAS NMR spectroscopy Methods 0.000 description 1
229920001410 Microfiber Polymers 0.000 description 1
239000012901 Milli-Q water Substances 0.000 description 1
241000221960 Neurospora Species 0.000 description 1
108091028043 Nucleic acid sequence Proteins 0.000 description 1
241000218657 Picea Species 0.000 description 1
239000004793 Polystyrene Substances 0.000 description 1
108010076504 Protein Sorting Signals Proteins 0.000 description 1
241000228453 Pyrenophora Species 0.000 description 1
241000221662 Sclerotinia Species 0.000 description 1
238000012300 Sequence Analysis Methods 0.000 description 1
VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
241000187747 Streptomyces Species 0.000 description 1
UGXQOOQUZRUVSS-ZZXKWVIFSA-N [5-[3,5-dihydroxy-2-(1,3,4-trihydroxy-5-oxopentan-2-yl)oxyoxan-4-yl]oxy-3,4-dihydroxyoxolan-2-yl]methyl (e)-3-(4-hydroxyphenyl)prop-2-enoate Chemical compound OC1C(OC(CO)C(O)C(O)C=O)OCC(O)C1OC1C(O)C(O)C(COC(=O)\C=C\C=2C=CC(O)=CC=2)O1 UGXQOOQUZRUVSS-ZZXKWVIFSA-N 0.000 description 1
238000002835 absorbance Methods 0.000 description 1
UGAPHEBNTGUMBB-UHFFFAOYSA-N acetic acid;ethyl acetate Chemical compound CC(O)=O.CCOC(C)=O UGAPHEBNTGUMBB-UHFFFAOYSA-N 0.000 description 1
IAJILQKETJEXLJ-RSJOWCBRSA-N aldehydo-D-galacturonic acid Chemical compound O=C[C@H](O)[C@@H](O)[C@@H](O)[C@H](O)C(O)=O IAJILQKETJEXLJ-RSJOWCBRSA-N 0.000 description 1
229910052783 alkali metal Inorganic materials 0.000 description 1
150000001340 alkali metals Chemical class 0.000 description 1
108010084650 alpha-N-arabinofuranosidase Proteins 0.000 description 1
229910052782 aluminium Inorganic materials 0.000 description 1
XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
238000003277 amino acid sequence analysis Methods 0.000 description 1
238000004873 anchoring Methods 0.000 description 1
229920000617 arabinoxylan Polymers 0.000 description 1
229960001230 asparagine Drugs 0.000 description 1
CKLJMWTZIZZHCS-REOHCLBHSA-N aspartic acid group Chemical group N[C@@H](CC(=O)O)C(=O)O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 1
QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical group [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
239000002585 base Substances 0.000 description 1
230000008901 benefit Effects 0.000 description 1
AEMOLEFTQBMNLQ-UHFFFAOYSA-N beta-D-galactopyranuronic acid Natural products OC1OC(C(O)=O)C(O)C(O)C1O AEMOLEFTQBMNLQ-UHFFFAOYSA-N 0.000 description 1
229920001222 biopolymer Polymers 0.000 description 1
230000015572 biosynthetic process Effects 0.000 description 1
229940098773 bovine serum albumin Drugs 0.000 description 1
108010059485 brain synaptic membrane glycoprotein gp 50 Proteins 0.000 description 1
239000006227 byproduct Substances 0.000 description 1
239000011575 calcium Substances 0.000 description 1
229910052791 calcium Inorganic materials 0.000 description 1
150000001669 calcium Chemical class 0.000 description 1
244000309466 calf Species 0.000 description 1
238000006555 catalytic reaction Methods 0.000 description 1
238000005119 centrifugation Methods 0.000 description 1
108010080434 cephalosporin-C deacetylase Proteins 0.000 description 1
230000008859 change Effects 0.000 description 1
238000004737 colorimetric analysis Methods 0.000 description 1
239000002131 composite material Substances 0.000 description 1
230000001143 conditioned effect Effects 0.000 description 1
238000010276 construction Methods 0.000 description 1
238000001816 cooling Methods 0.000 description 1
230000002079 cooperative effect Effects 0.000 description 1
229910052802 copper Inorganic materials 0.000 description 1
239000010949 copper Substances 0.000 description 1
238000012937 correction Methods 0.000 description 1
238000005388 cross polarization Methods 0.000 description 1
239000013078 crystal Substances 0.000 description 1
230000001461 cytolytic effect Effects 0.000 description 1
230000006196 deacetylation Effects 0.000 description 1
238000003381 deacetylation reaction Methods 0.000 description 1
230000001419 dependent effect Effects 0.000 description 1
IJKVHSBPTUYDLN-UHFFFAOYSA-N dihydroxy(oxo)silane Chemical compound O[Si](O)=O IJKVHSBPTUYDLN-UHFFFAOYSA-N 0.000 description 1
238000006073 displacement reaction Methods 0.000 description 1
239000012153 distilled water Substances 0.000 description 1
229940079593 drug Drugs 0.000 description 1
239000003814 drug Substances 0.000 description 1
150000002148 esters Chemical class 0.000 description 1
238000011156 evaluation Methods 0.000 description 1
KSEBMYQBYZTDHS-HWKANZROSA-N ferulic acid Chemical compound COC1=CC(\C=C\C(O)=O)=CC=C1O KSEBMYQBYZTDHS-HWKANZROSA-N 0.000 description 1
108010041969 feruloyl esterase Proteins 0.000 description 1
239000012065 filter cake Substances 0.000 description 1
239000012847 fine chemical Substances 0.000 description 1
235000019253 formic acid Nutrition 0.000 description 1
239000012458 free base Substances 0.000 description 1
229960003082 galactose Drugs 0.000 description 1
210000001035 gastrointestinal tract Anatomy 0.000 description 1
125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 1
229930182480 glucuronide Natural products 0.000 description 1
150000008134 glucuronides Chemical class 0.000 description 1
125000000291 glutamic acid group Chemical group N[C@@H](CCC(O)=O)C(=O)* 0.000 description 1
150000004676 glycans Polymers 0.000 description 1
239000001963 growth medium Substances 0.000 description 1
238000010438 heat treatment Methods 0.000 description 1
230000002573 hemicellulolytic effect Effects 0.000 description 1
150000002402 hexoses Chemical class 0.000 description 1
239000000017 hydrogel Substances 0.000 description 1
238000002329 infrared spectrum Methods 0.000 description 1
238000009434 installation Methods 0.000 description 1
238000001155 isoelectric focusing Methods 0.000 description 1
238000002955 isolation Methods 0.000 description 1
238000012417 linear regression Methods 0.000 description 1
238000011866 long-term treatment Methods 0.000 description 1
229940041290 mannose Drugs 0.000 description 1
238000011034 membrane dialysis Methods 0.000 description 1
238000005374 membrane filtration Methods 0.000 description 1
125000000956 methoxy group Chemical group [H]C([H])([H])O* 0.000 description 1
150000004702 methyl esters Chemical class 0.000 description 1
239000003658 microfiber Substances 0.000 description 1
230000004048 modification Effects 0.000 description 1
238000012986 modification Methods 0.000 description 1
235000015097 nutrients Nutrition 0.000 description 1
230000010494 opalescence Effects 0.000 description 1
239000002245 particle Substances 0.000 description 1
150000007965 phenolic acids Chemical class 0.000 description 1
235000009048 phenolic acids Nutrition 0.000 description 1
150000002989 phenols Chemical class 0.000 description 1
230000010287 polarization Effects 0.000 description 1
229920002223 polystyrene Polymers 0.000 description 1
229920002981 polyvinylidene fluoride Polymers 0.000 description 1
125000000075 primary alcohol group Chemical group 0.000 description 1
RYVMUASDIZQXAA-UHFFFAOYSA-N pyranoside Natural products O1C2(OCC(C)C(OC3C(C(O)C(O)C(CO)O3)O)C2)C(C)C(C2(CCC3C4(C)CC5O)C)C1CC2C3CC=C4CC5OC(C(C1O)O)OC(CO)C1OC(C1OC2C(C(OC3C(C(O)C(O)C(CO)O3)O)C(O)C(CO)O2)O)OC(CO)C(O)C1OC1OCC(O)C(O)C1O RYVMUASDIZQXAA-UHFFFAOYSA-N 0.000 description 1
238000011002 quantification Methods 0.000 description 1
238000012207 quantitative assay Methods 0.000 description 1
239000011541 reaction mixture Substances 0.000 description 1
230000009467 reduction Effects 0.000 description 1
239000012925 reference material Substances 0.000 description 1
238000001226 reprecipitation Methods 0.000 description 1
230000008684 selective degradation Effects 0.000 description 1
SBIBMFFZSBJNJF-UHFFFAOYSA-N selenium;zinc Chemical compound [Se]=[Zn] SBIBMFFZSBJNJF-UHFFFAOYSA-N 0.000 description 1
238000000926 separation method Methods 0.000 description 1
238000007873 sieving Methods 0.000 description 1
239000000741 silica gel Substances 0.000 description 1
229910002027 silica gel Inorganic materials 0.000 description 1
238000005549 size reduction Methods 0.000 description 1
239000001632 sodium acetate Substances 0.000 description 1
235000017281 sodium acetate Nutrition 0.000 description 1
241000894007 species Species 0.000 description 1
230000003595 spectral effect Effects 0.000 description 1
238000010561 standard procedure Methods 0.000 description 1
238000003756 stirring Methods 0.000 description 1
125000001424 substituent group Chemical group 0.000 description 1
229910052717 sulfur Inorganic materials 0.000 description 1
239000011593 sulfur Substances 0.000 description 1
239000006228 supernatant Substances 0.000 description 1
230000008961 swelling Effects 0.000 description 1
230000002195 synergetic effect Effects 0.000 description 1
ZZIZZTHXZRDOFM-XFULWGLBSA-N tamsulosin hydrochloride Chemical compound [H+].[Cl-].CCOC1=CC=CC=C1OCCN[C@H](C)CC1=CC=C(OC)C(S(N)(=O)=O)=C1 ZZIZZTHXZRDOFM-XFULWGLBSA-N 0.000 description 1
238000005809 transesterification reaction Methods 0.000 description 1
125000000430 tryptophan group Chemical group [H]N([H])C(C(=O)O*)C([H])([H])C1=C([H])N([H])C2=C([H])C([H])=C([H])C([H])=C12 0.000 description 1
UONOETXJSWQNOL-UHFFFAOYSA-N tungsten carbide Chemical group [W+]#[C-] UONOETXJSWQNOL-UHFFFAOYSA-N 0.000 description 1
235000002374 tyrosine Nutrition 0.000 description 1
125000001493 tyrosinyl group Chemical class [H]OC1=C([H])C([H])=C(C([H])=C1[H])C([H])([H])C([H])(N([H])[H])C(*)=O 0.000 description 1
238000000108 ultra-filtration Methods 0.000 description 1
238000011179 visual inspection Methods 0.000 description 1
238000005406 washing Methods 0.000 description 1
238000003809 water extraction Methods 0.000 description 1
210000000051 wattle Anatomy 0.000 description 1
238000005303 weighing Methods 0.000 description 1
239000007166 xylan medium Substances 0.000 description 1
229960003487 xylose Drugs 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01139—Alpha-glucuronidase (3.2.1.139)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)

Landscapes

Chemical & Material Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Health & Medical Sciences (AREA)
Organic Chemistry (AREA)
Engineering & Computer Science (AREA)
Bioinformatics & Cheminformatics (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Genetics & Genomics (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
General Engineering & Computer Science (AREA)
Medicinal Chemistry (AREA)
Molecular Biology (AREA)
Biomedical Technology (AREA)
Biotechnology (AREA)
Microbiology (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Enzymes And Modification Thereof (AREA)

SE1150989A 2009-03-31 2010-03-31 Förfarande för enzymatisk hydrolysering av glukuronoxylan SE537440C2 (sv)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
ZA200908321		2009-03-31
PCT/IB2010/000724 WO2010113020A1 (en)	2009-03-31	2010-03-31	An enzyme with alpha-glucuronidase activity

Publications (2)

Publication Number	Publication Date
SE1150989A1 SE1150989A1 (sv)	2011-12-21
SE537440C2 true SE537440C2 (sv)	2015-04-28

Family

ID=42827523

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
SE1150989A SE537440C2 (sv)	2009-03-31	2010-03-31	Förfarande för enzymatisk hydrolysering av glukuronoxylan

Country Status (5)

Country	Link
BR (1)	BRPI1006779A2 (pt)
FI (1)	FI126843B (pt)
SE (1)	SE537440C2 (pt)
WO (1)	WO2010113020A1 (pt)
ZA (1)	ZA201107541B (pt)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US20130089592A1 (en) *	2010-06-08	2013-04-11	Stellenbosch University	Method of producing a hydrogel
PL3686280T3 (pl)	2013-02-04	2022-09-19	Dsm Ip Assets B.V.	Polipeptyd degradujący węglowodany i jego zastosowania

2010
- 2010-03-31 WO PCT/IB2010/000724 patent/WO2010113020A1/en not_active Ceased
- 2010-03-31 SE SE1150989A patent/SE537440C2/sv not_active IP Right Cessation
- 2010-03-31 BR BRPI1006779A patent/BRPI1006779A2/pt not_active IP Right Cessation
2011
- 2011-10-14 ZA ZA2011/07541A patent/ZA201107541B/en unknown
- 2011-10-28 FI FI20116061A patent/FI126843B/fi not_active IP Right Cessation

Also Published As

Publication number	Publication date
BRPI1006779A2 (pt)	2019-09-24
FI126843B (fi)	2017-06-15
FI20116061L (fi)	2011-10-28
ZA201107541B (en)	2012-11-28
WO2010113020A1 (en)	2010-10-07
SE1150989A1 (sv)	2011-12-21

Legal Events

Date	Code	Title	Description
2018-12-04	NUG	Patent has lapsed

Publication	Publication Date	Title
EP2580249A1 (en)	2013-04-17	Modification of xylan
Akpinar et al.	2009	Production of xylooligosaccharides by controlled acid hydrolysis of lignocellulosic materials
Khaleghipour et al.	2021	Extraction of sugarcane bagasse arabinoxylan, integrated with enzymatic production of xylo-oligosaccharides and separation of cellulose
EP2796561B1 (en)	2016-07-20	Method for producing lignin degradation product
Qing et al.	2013	Xylooligosaccharides production, quantification, and characterization in context of lignocellulosic biomass pretreatment
CN109641973B (zh)	2022-03-08	Gh10木聚糖酶、gh62阿拉伯呋喃糖苷酶、研磨方法以及其他应用
AU2011325841B2 (en)	2016-01-07	Bagasse fractionation for cellulosic ethanol and chemical production
Gullón et al.	2010	Production, refining, structural characterization and fermentability of rice husk xylooligosaccharides
EA029050B1 (ru)	2018-02-28	Способ ферментативного гидролиза лигноцеллюлозного материала и ферментации сахаров
EP3438263A1 (en)	2019-02-06	Trichoderma fungus having mutant-type bxl1 gene and method for producing xylooligosaccharide and glucose by using same
Munk et al.	2020	Selective enzymatic release and gel formation by cross-linking of feruloylated glucurono-arabinoxylan from corn bran
Mallek-Fakhfakh et al.	2017	Enzymatic hydrolysis of pretreated Alfa fibers (Stipa tenacissima) using β-d-glucosidase and xylanase of Talaromyces thermophilus from solid-state fermentation
Chimphango et al.	2012	Isolation, characterization and enzymatic modification of water soluble xylans from Eucalyptus grandis wood and sugarcane bagasse
Makarova et al.	2014	Enzymatic hydrolysis of cellulose from oat husks at different substrate concentrations
Xue et al.	2023	Characterization of the recombinant GH10 xylanase from Trichoderma orientalis EU7-22 and its synergistic hydrolysis of bamboo hemicellulose with α-glucuronidase and α-L-arabinofuranosidase
Li et al.	2025	Synergy of GH67 and GH115 α-1, 2-glucuronidases with Penicillium subrubescens endoxylanases to stimulate xylooligosaccharide production
SE537440C2 (sv)	2015-04-28	Förfarande för enzymatisk hydrolysering av glukuronoxylan
EP2643357B1 (en)	2017-02-22	A method to increase the molecular weight of wood mannans and xylans comprising aromatic moieties
Zhou et al.	2010	Effect of hot water pretreatment severity on the degradation and enzymatic hydrolysis of corn stover
EP2307554B1 (en)	2013-06-26	Process for oxidizing lignocellulosic material employing a Marasmius scorodonius peroxidase
Akpinar et al.	2010	Enzymatic processing and antioxidant activity of agricultural waste autohydrolysis liquors.
Murano et al.	1993	Evaluation of steam explosion as pretreatment in agar extraction from Gracilaria dura (C. Agardh) J. Agardh (Gracilariaceae, Rhodophyta)
US9845365B2 (en)	2017-12-19	Method for making pentoses and pentose-based soluble oligo/polysaccharides from cereal grain involving debranning technology
Han et al.	2020	Effect of [EMIM] Ac recycling on Salix gracilistyla Miq. pretreatment for enzymatic saccharification
CN107699551B (zh)	2021-02-05	一种β-葡萄糖苷酶SPBGL5在水解木聚糖多糖类物质中的应用