SE8505568L - EXPRESSION VECTOR AND METHOD OF PRODUCING THEREOF - Google Patents

EXPRESSION VECTOR AND METHOD OF PRODUCING THEREOF

Info

Publication number
SE8505568L
SE8505568L SE8505568A SE8505568A SE8505568L SE 8505568 L SE8505568 L SE 8505568L SE 8505568 A SE8505568 A SE 8505568A SE 8505568 A SE8505568 A SE 8505568A SE 8505568 L SE8505568 L SE 8505568L
Authority
SE
Sweden
Prior art keywords
operon
hybrid
coding
rrna
expression vector
Prior art date
Application number
SE8505568A
Other languages
Unknown language ( )
Swedish (sv)
Other versions
SE8505568D0 (en
Inventor
I Boros
P Venetianer
T Lukacsovich
Original Assignee
Richter Gedeon Vegyeszet
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Richter Gedeon Vegyeszet filed Critical Richter Gedeon Vegyeszet
Publication of SE8505568D0 publication Critical patent/SE8505568D0/en
Publication of SE8505568L publication Critical patent/SE8505568L/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Toxicology (AREA)
  • Endocrinology (AREA)
  • Diabetes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

An expression vector comprising a hybrid operon which combines parts of an E.coli rRNA operon and a part of a protein coding operon, which may be constructed by removing most of the structural part of an E.coli rRNA operon carried by a suitable plasmid to obtain a shortened rRNA operon, combining said shortened rRNA operon with a part of a protein-coding operon to produce an effective hybrid expression control region and finally inserting a DNA fragment coding for a desired polypeptide into the combined operon to ensure gene expression under the control of said hybrid expression control region.
SE8505568A 1984-11-26 1985-11-25 EXPRESSION VECTOR AND METHOD OF PRODUCING THEREOF SE8505568L (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
HU844363A HU194309B (en) 1984-11-26 1984-11-26 Process for producing expressive vectors

Publications (2)

Publication Number Publication Date
SE8505568D0 SE8505568D0 (en) 1985-11-25
SE8505568L true SE8505568L (en) 1986-05-27

Family

ID=10967756

Family Applications (1)

Application Number Title Priority Date Filing Date
SE8505568A SE8505568L (en) 1984-11-26 1985-11-25 EXPRESSION VECTOR AND METHOD OF PRODUCING THEREOF

Country Status (15)

Country Link
JP (1) JPS61166395A (en)
AU (1) AU5034785A (en)
BE (1) BE903695A (en)
DE (1) DE3541783A1 (en)
DK (1) DK547285A (en)
FI (1) FI854655L (en)
FR (1) FR2573773A1 (en)
GB (1) GB2167760A (en)
HU (1) HU194309B (en)
IL (1) IL77152A0 (en)
IT (1) IT1191642B (en)
NL (1) NL8503257A (en)
PL (1) PL256453A1 (en)
SE (1) SE8505568L (en)
ZA (1) ZA859033B (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2592541Y2 (en) * 1991-02-15 1999-03-24 愛知機械工業株式会社 Lubrication structure of manual transmission

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5756495A (en) * 1980-09-24 1982-04-05 Kyowa Hakko Kogyo Co Ltd Novel dna-introduction vector and recombinant dna
IL65775A (en) * 1981-05-18 1985-05-31 Genentech Inc Microbial hybrid promotors and plasmids and e.coli comprising them
GB2135678B (en) * 1983-02-17 1986-08-28 Univ New York State Res Found Ribosomal rna operon and method of incorporating same in plasmids and bacteria

Also Published As

Publication number Publication date
ZA859033B (en) 1986-08-27
BE903695A (en) 1986-05-22
DE3541783A1 (en) 1986-06-12
FI854655A7 (en) 1986-05-27
HUT39475A (en) 1986-09-29
FI854655L (en) 1986-05-27
GB8529127D0 (en) 1986-01-02
DK547285A (en) 1986-05-27
FR2573773A1 (en) 1986-05-30
SE8505568D0 (en) 1985-11-25
AU5034785A (en) 1986-06-05
HU194309B (en) 1988-01-28
GB2167760A (en) 1986-06-04
IT8522982A0 (en) 1985-11-26
PL256453A1 (en) 1986-12-30
IL77152A0 (en) 1986-04-29
JPS61166395A (en) 1986-07-28
NL8503257A (en) 1986-06-16
FI854655A0 (en) 1985-11-26
IT1191642B (en) 1988-03-23
DK547285D0 (en) 1985-11-26

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Effective date: 19871030