SE8600011L - METHOD OF IDENTIFICATION OF NUCLEIC ACIDS - Google Patents

METHOD OF IDENTIFICATION OF NUCLEIC ACIDS

Info

Publication number
SE8600011L
SE8600011L SE8600011A SE8600011A SE8600011L SE 8600011 L SE8600011 L SE 8600011L SE 8600011 A SE8600011 A SE 8600011A SE 8600011 A SE8600011 A SE 8600011A SE 8600011 L SE8600011 L SE 8600011L
Authority
SE
Sweden
Prior art keywords
probe
identification
nucleic acids
hybridization
biotin
Prior art date
Application number
SE8600011A
Other languages
Unknown language ( )
Swedish (sv)
Other versions
SE463212B (en
SE8600011D0 (en
Inventor
H E Soderlund
Original Assignee
Orion Yhtymae Oy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Orion Yhtymae Oy filed Critical Orion Yhtymae Oy
Publication of SE8600011D0 publication Critical patent/SE8600011D0/en
Publication of SE8600011L publication Critical patent/SE8600011L/en
Publication of SE463212B publication Critical patent/SE463212B/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Saccharide Compounds (AREA)

Abstract

A hybridization method for the identification of nucleic acids in solution is presented. At least two probes, a detector probe and a capturing probe are used in the method. The detector probe is labelled with a detectable label and to the capturing probe a member of an affinity pair is attached. The capturing probe: target nucleic acid: detector probe hybrid formed in the hybridization reaction is isolated by the aid of the other member of the affinity pair. Suitable affinity pairs are biotin-avidin or biotin-streptavidin homopolynucleotides, antigens-antibodies etc. The method is faster to perform than the traditional sandwich hybridization.
SE8600011A 1985-01-02 1986-01-02 METHOD FOR IDENTIFYING NUCLEIC ACIDS SE463212B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FI850004A FI72146C (en) 1985-01-02 1985-01-02 Procedure for Identifying Nucleic Acids.

Publications (3)

Publication Number Publication Date
SE8600011D0 SE8600011D0 (en) 1986-01-02
SE8600011L true SE8600011L (en) 1986-07-03
SE463212B SE463212B (en) 1990-10-22

Family

ID=8520136

Family Applications (1)

Application Number Title Priority Date Filing Date
SE8600011A SE463212B (en) 1985-01-02 1986-01-02 METHOD FOR IDENTIFYING NUCLEIC ACIDS

Country Status (21)

Country Link
JP (1) JPH0669400B2 (en)
AT (1) AT397514B (en)
AU (1) AU561382B2 (en)
BE (1) BE903937A (en)
CA (1) CA1271705A (en)
CH (1) CH666696A5 (en)
DE (1) DE3546312A1 (en)
DK (1) DK164932C (en)
FI (1) FI72146C (en)
FR (1) FR2575493B1 (en)
GB (1) GB2169403B (en)
HU (1) HU196453B (en)
IE (1) IE58290B1 (en)
IL (1) IL77489A (en)
IT (1) IT1201514B (en)
LU (1) LU86238A1 (en)
NL (1) NL189427C (en)
NO (1) NO166743C (en)
RO (1) RO94651B (en)
SE (1) SE463212B (en)
ZA (1) ZA859895B (en)

Families Citing this family (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5288609A (en) * 1984-04-27 1994-02-22 Enzo Diagnostics, Inc. Capture sandwich hybridization method and composition
US6060237A (en) 1985-02-26 2000-05-09 Biostar, Inc. Devices and methods for optical detection of nucleic acid hybridization
IL79112A (en) * 1985-06-13 1992-01-15 Abbott Lab Method for the isolation of a nucleic acid sequence and kit for performing a hybridization assay
US4868105A (en) * 1985-12-11 1989-09-19 Chiron Corporation Solution phase nucleic acid sandwich assay
US4910300A (en) * 1985-12-11 1990-03-20 Chiron Corporation Method for making nucleic acid probes
US4882269A (en) * 1985-12-13 1989-11-21 Princeton University Amplified hybridization assay
FI76119C (en) * 1986-02-27 1988-09-09 Orion Yhtymae Oy QUANTITATIVE BEARING OF NUCLEIN SYRAMOLEKYLER AND REAGENTS FOERPACKNING SOM ANVAENDS VID FOERFARANDET.
AU622104B2 (en) * 1987-03-11 1992-04-02 Sangtec Molecular Diagnostics Ab Method of assaying of nucleic acids, a reagent combination and kit therefore
EP0365595A4 (en) * 1987-06-26 1990-06-05 Du Pont SEPARATION OF CONTAMINATED SEQUENCES FROM RECOMBINANT CLONED DNA USING AFFINITY PARTICLES.
DE3851810T2 (en) * 1987-07-31 1995-02-09 Gen Probe Inc Polynucleotide test using oligonucleotides to eliminate unwanted cross-reactions.
EP0305145A3 (en) * 1987-08-24 1990-05-02 Ortho Diagnostic Systems Inc. Methods and probes for detecting nucleic acids
EP0304845A3 (en) * 1987-08-28 1991-03-06 Profile Diagnostic Sciences Inc. Method and kit for assaying gene expressions
CA1325761C (en) * 1987-12-25 1994-01-04 Takanori Oka Method of detecting an intended nucleic acid in a sample
DE3800644A1 (en) * 1988-01-12 1989-07-20 Boehringer Mannheim Gmbh PROCESS FOR HIGHLY SPECIFIC DETECTION OF NUCLEIC ACIDS IN SOLUTION
US5104791A (en) * 1988-02-09 1992-04-14 E. I. Du Pont De Nemours And Company Particle counting nucleic acid hybridization assays
EP0436547B1 (en) * 1988-05-10 1994-11-30 E.I. Du Pont De Nemours And Company Process for rapid nucleic acid detection
US5185243A (en) * 1988-08-25 1993-02-09 Syntex (U.S.A.) Inc. Method for detection of specific nucleic acid sequences
AU629845B2 (en) * 1988-08-30 1992-10-15 Abbott Laboratories Detection and amplification of target nucleic acid sequences
US5858652A (en) * 1988-08-30 1999-01-12 Abbott Laboratories Detection and amplification of target nucleic acid sequences
GB2225112A (en) * 1988-11-22 1990-05-23 Ici Plc Hybridisation probes
US5082935A (en) * 1988-12-15 1992-01-21 Amoco Corporation Diagnostic reagents made by attaching cytidine containing nucleic acid probes to amino functionalized solid supports by bisulfite mediated transamination
EP0418346A1 (en) * 1989-02-06 1991-03-27 Gene-Trak Systems Probes and methods for the detection of listeria
WO1990010717A1 (en) * 1989-03-10 1990-09-20 Gene-Trak Systems Preventing interference with affinity capture schemes
ATE118824T1 (en) 1989-03-10 1995-03-15 Amoco Corp IMMOBILIZED OLIGONUCLEOTIDE PROBE AND USES THEREOF.
AU6075490A (en) * 1989-07-11 1991-02-06 Microprobe Corporation Quantification of bacteria using a nucleic acid hybridization assay
US5334501A (en) * 1989-07-11 1994-08-02 Microprobe Corporation Quantification of bacteria using a nucleic acid hybridization assay
GB8924989D0 (en) * 1989-11-06 1989-12-28 Scotgen Ltd Method and device for the detection of antibiotic resistance
US5580970A (en) * 1989-12-01 1996-12-03 Amoco Corporation Detection of HPV transcripts
AU7429591A (en) * 1990-04-18 1991-10-24 Gene-Trak Systems Nucleic acid probes for the detection of giardia lamblia
EP0529070A1 (en) * 1991-02-27 1993-03-03 Amoco Corporation Methods for improving the sensitivity of hybridization assays
WO1993020234A1 (en) * 1992-03-31 1993-10-14 E.I. Du Pont De Nemours And Company A rapid, high capacity nucleic acid based assay
US7713528B1 (en) 1993-02-18 2010-05-11 Enzo Therapeutics, Inc. Method for in vivo delivery of active compounds using reagent conjugate
RU2146707C1 (en) * 1996-04-11 2000-03-20 Куликова Валентина Филипповна Method of detection of analyzed sequence of nucleic acid
US5853993A (en) * 1996-10-21 1998-12-29 Hewlett-Packard Company Signal enhancement method and kit
GB0016833D0 (en) 2000-07-07 2000-08-30 Lee Helen Improved dipstick assays (2)
US7465540B2 (en) 2000-09-21 2008-12-16 Luminex Corporation Multiple reporter read-out for bioassays
CN1303221C (en) * 2003-01-27 2007-03-07 英科新创(厦门)科技有限公司 Method for detecting target nucleic acid using affinity amplifying integrated signal group

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI63596C (en) * 1981-10-16 1983-07-11 Orion Yhtymae Oy MICROBIA DIAGNOSIS FOERFARANDE SOM GRUNDAR SIG PAO SKIKTSHYBRIDISERING AV NUCLEINSYROR OCH VID FOERFARANDET ANVAENDA KOMBINATIONER AV REAGENSER
FR2518755B1 (en) * 1981-12-23 1986-04-11 Pasteur Institut PROBE CONTAINING MODIFIED NUCLEIC ACID RECOGNIZABLE BY SPECIFIC ANTIBODIES AND USE OF THE SAME TO DETECT AND CHARACTERIZE A HOMOLOGATED DNA SEQUENCE
GB8306426D0 (en) * 1983-03-09 1983-04-13 Malcolm A D B Detecting polynucleotide sequence
CA1228811A (en) * 1983-05-05 1987-11-03 Robert G. Pergolizzi Assay method utilizing polynucleotide sequences
NO843838L (en) * 1983-09-26 1985-03-27 Ortho Diagnostic Systems Inc PROCEDURE FOR DETECTING NUCLEIC ACID
ZA849595B (en) * 1983-12-12 1985-07-31 Miles Lab Hybridization assay employing labeled probe and anti-hybrid
FR2558172B1 (en) * 1984-01-16 1986-06-13 Inst Nat Sante Rech Med PROBE CONTAINING MODIFIED NUCLEIC ACID AND RECOGNITION BY SPECIFIC ANTIBODIES AND USE OF SUCH PROBE AND THESE SPECIFIC ANTIBODIES TO DETECT AND CHARACTERIZE A HOMOLOGATED DNA SEQUENCE
CA1223222A (en) * 1984-02-22 1987-06-23 Nanibhushan Dattagupta Immobilized nucleic acid-containing probes
FR2560298B1 (en) * 1984-02-28 1988-07-15 Mors METHOD AND DEVICE FOR ADJUSTING THE PRECISION OF THE PRESSURE DROP OF A FLUID INITIALLY AT HIGH PRESSURE, SUCH AS A HYDRAULIC FLUID FOR EXAMPLE SUPPLYING A HYDRAULIC GROUP, AND APPLICATION OF THE METHOD TO THE DEVICE IN A HYDRAULIC GROUP OR PLANT INTEGRATED OR NOT, ESPECIALLY IN A MACHINE TABLE OR ANY TOOL SUPPORT DEVICE
NZ211453A (en) * 1984-03-22 1989-01-06 Biotechnology Research Enterpr Aryl azides, their preparation and use in the detection, localisation and isolation of polynucleotides
US4766062A (en) * 1984-05-07 1988-08-23 Allied Corporation Displacement polynucleotide assay method and polynucleotide complex reagent therefor

Also Published As

Publication number Publication date
SE463212B (en) 1990-10-22
GB2169403A (en) 1986-07-09
DK164932C (en) 1993-01-25
AU561382B2 (en) 1987-05-07
SE8600011D0 (en) 1986-01-02
DE3546312C2 (en) 1992-08-06
NO166743B (en) 1991-05-21
IT8523408A0 (en) 1985-12-30
FI850004A0 (en) 1985-01-02
DK386D0 (en) 1986-01-02
JPS61185200A (en) 1986-08-18
BE903937A (en) 1986-04-16
CA1271705A (en) 1990-07-17
DK386A (en) 1986-07-03
ZA859895B (en) 1986-08-27
IL77489A (en) 1991-01-31
CH666696A5 (en) 1988-08-15
GB8531414D0 (en) 1986-02-05
IE853333L (en) 1986-07-02
AT397514B (en) 1994-04-25
NO855308L (en) 1986-07-03
HU196453B (en) 1988-11-28
FR2575493A1 (en) 1986-07-04
NL189427C (en) 1993-04-01
NL8503597A (en) 1986-08-01
AU5174885A (en) 1986-07-17
RO94651B (en) 1988-07-01
FI72146C (en) 1987-04-13
FI72146B (en) 1986-12-31
IT1201514B (en) 1989-02-02
IE58290B1 (en) 1993-08-25
RO94651A (en) 1988-06-30
FI850004L (en) 1986-07-03
FR2575493B1 (en) 1990-01-26
DE3546312A1 (en) 1986-07-10
LU86238A1 (en) 1986-04-14
NL189427B (en) 1992-11-02
ATA376785A (en) 1993-09-15
GB2169403B (en) 1988-06-08
JPH0669400B2 (en) 1994-09-07
HUT40166A (en) 1986-11-28
DK164932B (en) 1992-09-07
NO166743C (en) 1991-08-28

Similar Documents

Publication Publication Date Title
SE8600011D0 (en) METHOD OF IDENTIFICATION OF NUCLEIC ACIDS
DK0407789T3 (en) Method for detecting nucleic acids
FI900602A0 (en) BESTAEMNING.
ATE40572T1 (en) PHOTOCHEMICAL METHOD FOR LABELING NUCLEIC ACIDS FOR DETECTION IN HYBRIDISATION SAMPLES.
BR8901136A (en) PROCESS TO DETECT THE PRESENCE OR ABSENCE OF AT LEAST ONE VARIANT NUCLEOTIDE IN ONE OR MORE NUCLEIC ACIDS, SEQUENCE OF NUCLEOTIDEOS FOR USE IN THE PROCESS AND CASE TO CARRY OUT THAT DETECTION
RO86356A (en) MICROBIENT IDENTIFICATION METHOD
EP0153873A3 (en) Detecting polynucleotide sequences
DK0408918T3 (en) Method for Detecting Nucleic Acids
DK163383C (en) METHOD AND REAGENT SYSTEM FOR DETERMINING DETECTIVE DETECTION OF A PARTICULAR POLYNUCLEOTIDE SEQUENCE IN A TESTING MEDIUM CONTAINING SINGLE STRUCTURED NUCLEIC ACIDS
EP0305145A3 (en) Methods and probes for detecting nucleic acids
ES2052607T3 (en) METHODS AND KITS TO DETECT NUCLEIC ACID SEQUENCES.
EP0366813A4 (en) Method for diagnosis of infectious diseases and method of detecting and identifying microorganisms
DE59105402D1 (en) METHOD FOR THE SENSITIVE DETECTION OF NUCLEIC ACIDS.

Legal Events

Date Code Title Description
NAL Patent in force

Ref document number: 8600011-4

Format of ref document f/p: F

NUG Patent has lapsed