SK176397A3 - A method for immobilization of enzymes - Google Patents

A method for immobilization of enzymes Download PDF

Info

Publication number: SK176397A3
Authority: SK; Slovakia
Prior art keywords: enzyme; lipase; immobilized; carrier; aqueous phase
Prior art date: 1995-06-27

Application number

SK1763-97A

Other languages

English (en)

Slovak (sk)

Inventor

Martin R Grote

Johan P T Geurtsen

Putte Karel P A M Van

Original Assignee

Unilever Nv

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1995-06-27

Filing date

1996-06-14

Publication date

1998-07-08

1996-06-14 Application filed by Unilever Nv filed Critical Unilever Nv

1998-07-08 Publication of SK176397A3 publication Critical patent/SK176397A3/sk

Links

102000004190 Enzymes Human genes 0.000 title claims abstract description 82
108090000790 Enzymes Proteins 0.000 title claims abstract description 82
238000000034 method Methods 0.000 title claims abstract description 40
108090001060 Lipase Proteins 0.000 claims abstract description 58
102000004882 Lipase Human genes 0.000 claims abstract description 58
239000004367 Lipase Substances 0.000 claims abstract description 57
235000019421 lipase Nutrition 0.000 claims abstract description 57
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 26
239000008346 aqueous phase Substances 0.000 claims abstract description 24
108010093096 Immobilized Enzymes Proteins 0.000 claims abstract description 23
230000008569 process Effects 0.000 claims abstract description 22
238000000855 fermentation Methods 0.000 claims abstract description 20
230000004151 fermentation Effects 0.000 claims abstract description 20
239000012071 phase Substances 0.000 claims abstract description 19
UFTFJSFQGQCHQW-UHFFFAOYSA-N triformin Chemical compound O=COCC(OC=O)COC=O UFTFJSFQGQCHQW-UHFFFAOYSA-N 0.000 claims abstract description 19
239000007787 solid Substances 0.000 claims abstract description 18
230000002209 hydrophobic effect Effects 0.000 claims abstract description 16
108010064785 Phospholipases Proteins 0.000 claims abstract description 15
102000015439 Phospholipases Human genes 0.000 claims abstract description 15
238000009884 interesterification Methods 0.000 claims abstract description 14
239000000839 emulsion Substances 0.000 claims abstract description 12
239000002245 particle Substances 0.000 claims abstract description 9
PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 26
150000003904 phospholipids Chemical class 0.000 claims description 17
238000006243 chemical reaction Methods 0.000 claims description 16
230000007062 hydrolysis Effects 0.000 claims description 16
238000006460 hydrolysis reaction Methods 0.000 claims description 16
239000000126 substance Substances 0.000 claims description 11
ZIIUUSVHCHPIQD-UHFFFAOYSA-N 2,4,6-trimethyl-N-[3-(trifluoromethyl)phenyl]benzenesulfonamide Chemical compound CC1=CC(C)=CC(C)=C1S(=O)(=O)NC1=CC=CC(C(F)(F)F)=C1 ZIIUUSVHCHPIQD-UHFFFAOYSA-N 0.000 claims description 10
238000002360 preparation method Methods 0.000 claims description 10
150000003626 triacylglycerols Chemical class 0.000 claims description 9
235000014113 dietary fatty acids Nutrition 0.000 claims description 8
229930195729 fatty acid Natural products 0.000 claims description 8
239000000194 fatty acid Substances 0.000 claims description 8
150000004665 fatty acids Chemical class 0.000 claims description 8
OGBUMNBNEWYMNJ-UHFFFAOYSA-N batilol Chemical class CCCCCCCCCCCCCCCCCCOCC(O)CO OGBUMNBNEWYMNJ-UHFFFAOYSA-N 0.000 claims description 5
230000003197 catalytic effect Effects 0.000 claims description 4
238000002156 mixing Methods 0.000 claims description 4
241000223258 Thermomyces lanuginosus Species 0.000 claims description 3
-1 diglycerides Chemical class 0.000 claims description 3
230000003100 immobilizing effect Effects 0.000 claims description 3
235000000346 sugar Nutrition 0.000 claims description 3
150000008163 sugars Chemical group 0.000 claims description 3
239000006228 supernatant Substances 0.000 claims description 3
229920002307 Dextran Polymers 0.000 claims description 2
241000235403 Rhizomucor miehei Species 0.000 claims description 2
241000235545 Rhizopus niveus Species 0.000 claims description 2
229920002472 Starch Polymers 0.000 claims description 2
239000001913 cellulose Substances 0.000 claims description 2
229920002678 cellulose Polymers 0.000 claims description 2
230000002255 enzymatic effect Effects 0.000 claims description 2
125000005313 fatty acid group Chemical group 0.000 claims description 2
235000019698 starch Nutrition 0.000 claims description 2
239000008107 starch Substances 0.000 claims description 2
239000003921 oil Substances 0.000 abstract description 37
239000007788 liquid Substances 0.000 abstract description 3
239000002028 Biomass Substances 0.000 abstract description 2
239000000463 material Substances 0.000 abstract 1
235000019198 oils Nutrition 0.000 description 34
235000021588 free fatty acids Nutrition 0.000 description 16
230000000694 effects Effects 0.000 description 12
238000005886 esterification reaction Methods 0.000 description 12
238000011065 in-situ storage Methods 0.000 description 11
238000004519 manufacturing process Methods 0.000 description 9
229920002125 Sokalan® Polymers 0.000 description 8
239000000203 mixture Substances 0.000 description 8
108010048733 Lipozyme Proteins 0.000 description 7
239000012530 fluid Substances 0.000 description 7
FCCDDURTIIUXBY-UHFFFAOYSA-N lipoamide Chemical compound NC(=O)CCCCC1CCSS1 FCCDDURTIIUXBY-UHFFFAOYSA-N 0.000 description 7
HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
239000003054 catalyst Substances 0.000 description 6
235000019626 lipase activity Nutrition 0.000 description 6
229940057917 medium chain triglycerides Drugs 0.000 description 6
230000009467 reduction Effects 0.000 description 5
238000001035 drying Methods 0.000 description 4
WRIDQFICGBMAFQ-UHFFFAOYSA-N (E)-8-Octadecenoic acid Natural products CCCCCCCCCC=CCCCCCCC(O)=O WRIDQFICGBMAFQ-UHFFFAOYSA-N 0.000 description 3
LQJBNNIYVWPHFW-UHFFFAOYSA-N 20:1omega9c fatty acid Natural products CCCCCCCCCCC=CCCCCCCCC(O)=O LQJBNNIYVWPHFW-UHFFFAOYSA-N 0.000 description 3
QSBYPNXLFMSGKH-UHFFFAOYSA-N 9-Heptadecensaeure Natural products CCCCCCCC=CCCCCCCCC(O)=O QSBYPNXLFMSGKH-UHFFFAOYSA-N 0.000 description 3
IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 3
RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 3
239000005642 Oleic acid Substances 0.000 description 3
ZQPPMHVWECSIRJ-UHFFFAOYSA-N Oleic acid Natural products CCCCCCCCC=CCCCCCCCC(O)=O ZQPPMHVWECSIRJ-UHFFFAOYSA-N 0.000 description 3
235000019486 Sunflower oil Nutrition 0.000 description 3
239000000969 carrier Substances 0.000 description 3
238000005119 centrifugation Methods 0.000 description 3
230000000052 comparative effect Effects 0.000 description 3
239000006185 dispersion Substances 0.000 description 3
230000032050 esterification Effects 0.000 description 3
QXJSBBXBKPUZAA-UHFFFAOYSA-N isooleic acid Natural products CCCCCCCC=CCCCCCCCCC(O)=O QXJSBBXBKPUZAA-UHFFFAOYSA-N 0.000 description 3
239000002808 molecular sieve Substances 0.000 description 3
VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 3
ZQPPMHVWECSIRJ-KTKRTIGZSA-N oleic acid Chemical compound CCCCCCCC\C=C/CCCCCCCC(O)=O ZQPPMHVWECSIRJ-KTKRTIGZSA-N 0.000 description 3
230000007420 reactivation Effects 0.000 description 3
238000000926 separation method Methods 0.000 description 3
URGAHOPLAPQHLN-UHFFFAOYSA-N sodium aluminosilicate Chemical compound [Na+].[Al+3].[O-][Si]([O-])=O.[O-][Si]([O-])=O URGAHOPLAPQHLN-UHFFFAOYSA-N 0.000 description 3
239000000758 substrate Substances 0.000 description 3
239000002600 sunflower oil Substances 0.000 description 3
FERIUCNNQQJTOY-UHFFFAOYSA-N Butyric acid Chemical compound CCCC(O)=O FERIUCNNQQJTOY-UHFFFAOYSA-N 0.000 description 2
OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
UYXTWWCETRIEDR-UHFFFAOYSA-N Tributyrin Chemical compound CCCC(=O)OCC(OC(=O)CCC)COC(=O)CCC UYXTWWCETRIEDR-UHFFFAOYSA-N 0.000 description 2
230000015572 biosynthetic process Effects 0.000 description 2
229910052799 carbon Inorganic materials 0.000 description 2
229910003460 diamond Inorganic materials 0.000 description 2
239000010432 diamond Substances 0.000 description 2
238000005516 engineering process Methods 0.000 description 2
239000003925 fat Substances 0.000 description 2
229910052757 nitrogen Inorganic materials 0.000 description 2
KJFMBFZCATUALV-UHFFFAOYSA-N phenolphthalein Chemical compound C1=CC(O)=CC=C1C1(C=2C=CC(O)=CC=2)C2=CC=CC=C2C(=O)O1 KJFMBFZCATUALV-UHFFFAOYSA-N 0.000 description 2
238000000746 purification Methods 0.000 description 2
239000012429 reaction media Substances 0.000 description 2
238000003756 stirring Methods 0.000 description 2
101000968489 Rhizomucor miehei Lipase Proteins 0.000 description 1
241000209140 Triticum Species 0.000 description 1
235000021307 Triticum Nutrition 0.000 description 1
239000002253 acid Substances 0.000 description 1
150000007513 acids Chemical class 0.000 description 1
239000012736 aqueous medium Substances 0.000 description 1
238000010923 batch production Methods 0.000 description 1
230000008901 benefit Effects 0.000 description 1
238000010924 continuous production Methods 0.000 description 1
238000007872 degassing Methods 0.000 description 1
238000009826 distribution Methods 0.000 description 1
238000004945 emulsification Methods 0.000 description 1
238000001704 evaporation Methods 0.000 description 1
238000002474 experimental method Methods 0.000 description 1
238000001914 filtration Methods 0.000 description 1
235000013312 flour Nutrition 0.000 description 1
235000013305 food Nutrition 0.000 description 1
238000004817 gas chromatography Methods 0.000 description 1
239000011521 glass Substances 0.000 description 1
150000004676 glycans Chemical class 0.000 description 1
125000005456 glyceride group Chemical group 0.000 description 1
239000012510 hollow fiber Substances 0.000 description 1
239000003262 industrial enzyme Substances 0.000 description 1
210000003734 kidney Anatomy 0.000 description 1
239000002609 medium Substances 0.000 description 1
238000002844 melting Methods 0.000 description 1
230000008018 melting Effects 0.000 description 1
239000012528 membrane Substances 0.000 description 1
230000002906 microbiologic effect Effects 0.000 description 1
244000005700 microbiome Species 0.000 description 1
230000007935 neutral effect Effects 0.000 description 1
QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen(.) Chemical compound [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
235000014593 oils and fats Nutrition 0.000 description 1
238000005192 partition Methods 0.000 description 1
229920001282 polysaccharide Polymers 0.000 description 1
239000005017 polysaccharide Substances 0.000 description 1
239000000843 powder Substances 0.000 description 1
238000012545 processing Methods 0.000 description 1
239000002994 raw material Substances 0.000 description 1
238000011084 recovery Methods 0.000 description 1
230000008929 regeneration Effects 0.000 description 1
238000011069 regeneration method Methods 0.000 description 1
230000000717 retained effect Effects 0.000 description 1
150000003839 salts Chemical class 0.000 description 1
230000003381 solubilizing effect Effects 0.000 description 1
239000002904 solvent Substances 0.000 description 1
238000010561 standard procedure Methods 0.000 description 1
238000003786 synthesis reaction Methods 0.000 description 1
238000005809 transesterification reaction Methods 0.000 description 1
238000012546 transfer Methods 0.000 description 1
125000005457 triglyceride group Chemical group 0.000 description 1
239000002699 waste material Substances 0.000 description 1
239000012610 weak anion exchange resin Substances 0.000 description 1
230000003442 weekly effect Effects 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6436—Fatty acid esters
- C12P7/6445—Glycerides
- C12P7/6458—Glycerides by transesterification, e.g. interesterification, ester interchange, alcoholysis or acidolysis
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6436—Fatty acid esters
- C12P7/6445—Glycerides
- C12P7/6454—Glycerides by esterification

Landscapes

Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Life Sciences & Earth Sciences (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Engineering & Computer Science (AREA)
Health & Medical Sciences (AREA)
Bioinformatics & Cheminformatics (AREA)
Genetics & Genomics (AREA)
Biochemistry (AREA)
Microbiology (AREA)
Biotechnology (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Biomedical Technology (AREA)
Oil, Petroleum & Natural Gas (AREA)
Chemical Kinetics & Catalysis (AREA)
General Chemical & Material Sciences (AREA)
Molecular Biology (AREA)
Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Fats And Perfumes (AREA)

SK1763-97A 1995-06-27 1996-06-14 A method for immobilization of enzymes SK176397A3 (en)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
EP95201738		1995-06-27
PCT/EP1996/002587 WO1997001632A1 (en)	1995-06-27	1996-06-14	Immobilized enzyme and its use for the processing of triglyceride oils

Publications (1)

Publication Number	Publication Date
SK176397A3 true SK176397A3 (en)	1998-07-08

Family

ID=8220419

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
SK1763-97A SK176397A3 (en)	1995-06-27	1996-06-14	A method for immobilization of enzymes

Country Status (13)

Country	Link
US (1)	US6162623A (de)
EP (1)	EP0835304A1 (de)
JP (1)	JP3403202B2 (de)
AU (1)	AU720776B2 (de)
BR (1)	BR9609381A (de)
CA (1)	CA2225475A1 (de)
CZ (1)	CZ286747B6 (de)
MX (1)	MX9800035A (de)
PL (1)	PL324288A1 (de)
SK (1)	SK176397A3 (de)
TR (1)	TR199701705T1 (de)
WO (1)	WO1997001632A1 (de)
ZA (1)	ZA965375B (de)

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KR20020043349A (ko) *	2000-12-04	2002-06-10	김병기	고정화 포스포리파제 ａ-2 및 이를 이용한 인지질 또는그를 포함하는 레시틴의 가수분해방법
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ES2461857T3 (es)	2004-06-16	2014-05-21	Dsm Ip Assets B.V.	Composiciones y métodos para la decoloración enzimática de clorofila
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CA2663001A1 (en)	2006-09-21	2008-03-27	Verenium Corporation	Phospholipases, nucleic acids encoding them and methods for making and using them
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GB0700074D0 (en) *	2007-01-03	2007-02-07	Danisco	process
IL180598A0 (en) *	2007-01-08	2007-07-04	Basheer Sobhi	Immobilized interfacial enzymes of improved and stabilized activity
TWI438279B (zh)	2007-03-16	2014-05-21	Nisshin Oillio Group Ltd	脂肪酶粉末製劑，其製造方法及使用
EP2158627A2 (de) *	2007-05-04	2010-03-03	Akermin, Inc.	Immobilisierte enzyme und ihre verwendungen
US20100216209A1 (en) *	2007-07-31	2010-08-26	Eiji Iwaoka	Immobilized lipase and method for producing the same
US8076123B2 (en) *	2007-10-26	2011-12-13	Oilseeds Biorefinery Corporation	Emulsification-free degumming of oil
AR077042A1 (es)	2009-06-12	2011-07-27	Danisco	Metodo para tratar una composicion que contiene pirofiofitina
UA111708C2 (uk)	2009-10-16	2016-06-10	Бандж Ойлз, Інк.	Спосіб рафінування олії
UA109884C2 (uk)	2009-10-16	2015-10-26		Поліпептид, що має активність ферменту фосфатидилінозитол-специфічної фосфоліпази с, нуклеїнова кислота, що його кодує, та спосіб його виробництва і застосування
WO2011110967A1 (en)	2010-03-12	2011-09-15	Danisco A/S	Process
BR112012032176A2 (pt)	2010-06-17	2015-09-08	Dupont Nutrition Biosci Aps	processo de tratamento de sementes que contém óleo, método de obtenção de óleo de sementes vegetais, processo de produção de um óleo vegetal refinado e óleo vegetal bruto ou refinado que pode ser obtido por meio de um método ou processo
DE102010025764A1 (de) *	2010-07-01	2012-01-05	Süd-Chemie AG	Phospholipase-Träger-Komplex
WO2012114232A1 (en)	2011-02-23	2012-08-30	Dupont Nutrition Biosciences Aps	Process
ES2649912T3 (es)	2011-02-23	2018-01-16	Dupont Nutrition Biosciences Aps	Tratamiento enzimático de la clorofila en los aceites vegetales
WO2013104660A1 (en)	2012-01-13	2013-07-18	Dupont Nutrition Biosciences Aps	Process for treating a plant oil comprising hydrolysing chlorophyll or a chlorophyll derivative and involving partial caustic neutralisation
WO2013104659A2 (en)	2012-01-13	2013-07-18	Dupont Nutrition Biosciences Aps	Process
WO2013160374A1 (en)	2012-04-27	2013-10-31	Dupont Nutrition Biosciences Aps	Process for refining crude plant oil involving enzymatic hydrolysis and gum recycling
WO2013160372A1 (en)	2012-04-27	2013-10-31	Dupont Nutrition Biosciences Aps	Process for treating plant oil involving addition of serial doses of chlorophyll or chlorophyll derivative degrading enzyme
US20150353970A1 (en)	2012-12-31	2015-12-10	Trans Bio-Diesel Ltd.	Enzymatic transesterification/esterification processing systems and processes employing lipases immobilzed on hydrophobic resins
CA2912755C (en) *	2013-06-27	2021-09-07	Nestec S.A.	Compositions and nutritional products with improved emulsion stability
US10294463B2 (en)	2013-07-03	2019-05-21	Keclon Sa	Modified Bacillus cereus phospholipase C protein and method of processing vegetable oil
ES3062708T3 (en)	2014-03-19	2026-04-13	Novozymes As	Polypeptides having phospholipase c activity and polynucleotides encoding same
EP3143135B1 (de)	2014-05-15	2019-04-10	Novozymes A/S	Zusammensetzungen mit polypeptiden mit phospholipase c-aktivität und verwendung davon
CN109628211A (zh) *	2018-12-26	2019-04-16	广东省农业科学院蚕业与农产品加工研究所	一种去除油脂中游离脂肪酸的方法
CN113355369B (zh) *	2020-03-02	2022-11-15	大连医诺生物股份有限公司	一种采用流加方式的固定化脂肪酶催化制备共轭亚油酸的方法及应用
CN116286731B (zh) *	2023-04-19	2024-06-11	中国农业科学院农产品加工研究所	蛋白基固定化酶及其制备方法及高效制备甘油二酯的方法

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GB8915299D0 (en) *	1989-07-04	1989-08-23	Cerestar Holding Bv	Carbohydrate refining process and a supported enzyme for use therein
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DE4027290C2 (de) *	1990-08-29	1995-03-23	Forschungszentrum Juelich Gmbh	Verfahren zur Gewinnung von Enzymen aus enzymhaltigen Suspensionen
US5182201A (en) *	1990-10-26	1993-01-26	Uop	Lipase immobilization without covalent bonding on an amphiphilic support containing lipophilic alkyl chains
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1996
- 1996-06-14 BR BR9609381A patent/BR9609381A/pt not_active Application Discontinuation
- 1996-06-14 EP EP96921996A patent/EP0835304A1/de not_active Withdrawn
- 1996-06-14 AU AU63036/96A patent/AU720776B2/en not_active Ceased
- 1996-06-14 SK SK1763-97A patent/SK176397A3/sk unknown
- 1996-06-14 WO PCT/EP1996/002587 patent/WO1997001632A1/en not_active Ceased
- 1996-06-14 US US08/983,085 patent/US6162623A/en not_active Expired - Fee Related
- 1996-06-14 CA CA002225475A patent/CA2225475A1/en not_active Abandoned
- 1996-06-14 TR TR97/01705T patent/TR199701705T1/xx unknown
- 1996-06-14 PL PL96324288A patent/PL324288A1/xx unknown
- 1996-06-14 JP JP50413097A patent/JP3403202B2/ja not_active Expired - Fee Related
- 1996-06-14 CZ CZ19974187A patent/CZ286747B6/cs not_active IP Right Cessation
- 1996-06-25 ZA ZA9605375A patent/ZA965375B/xx unknown
1998
- 1998-01-07 MX MX9800035A patent/MX9800035A/es unknown

Also Published As

Publication number	Publication date
CA2225475A1 (en)	1997-01-16
EP0835304A1 (de)	1998-04-15
CZ286747B6 (en)	2000-06-14
MX9800035A (es)	1998-11-29
BR9609381A (pt)	1999-05-18
JPH11500319A (ja)	1999-01-12
US6162623A (en)	2000-12-19
CZ418797A3 (cs)	1998-06-17
TR199701705T1 (xx)	1998-04-21
PL324288A1 (en)	1998-05-11
AU720776B2 (en)	2000-06-08
ZA965375B (en)	1997-12-25
WO1997001632A1 (en)	1997-01-16
JP3403202B2 (ja)	2003-05-06
AU6303696A (en)	1997-01-30

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