SMT201700295T1 - Ceppo batterico ospite che esprime dsbc ricombinante ed avente attività tsp ridotta - Google Patents
Ceppo batterico ospite che esprime dsbc ricombinante ed avente attività tsp ridottaInfo
- Publication number
- SMT201700295T1 SMT201700295T1 SM20170295T SMT201700295T SMT201700295T1 SM T201700295 T1 SMT201700295 T1 SM T201700295T1 SM 20170295 T SM20170295 T SM 20170295T SM T201700295 T SMT201700295 T SM T201700295T SM T201700295 T1 SMT201700295 T1 SM T201700295T1
- Authority
- SM
- San Marino
- Prior art keywords
- bacterial host
- host strain
- expressing recombinant
- strain expressing
- recombinant dsbc
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
- C07K14/245—Escherichia (G)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/24—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
- C07K16/241—Tumor Necrosis Factors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/90—Isomerases (5.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y503/00—Intramolecular oxidoreductases (5.3)
- C12Y503/04—Intramolecular oxidoreductases (5.3) transposing S-S bonds (5.3.4)
- C12Y503/04001—Protein disulfide-isomerase (5.3.4.1), i.e. disufide bond-forming enzyme
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
- C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/55—Fab or Fab'
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Gastroenterology & Hepatology (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB1000591.6A GB201000591D0 (en) | 2010-01-14 | 2010-01-14 | Bacterial hoist strain |
| PCT/EP2011/050416 WO2011086139A1 (en) | 2010-01-14 | 2011-01-13 | Bacterial host strain expressing recombinant dsbc and having reduced tsp activity |
| EP11701045.4A EP2523969B1 (en) | 2010-01-14 | 2011-01-13 | Bacterial host strain expressing recombinant dsbc and having reduced tsp activity |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SMT201700295T1 true SMT201700295T1 (it) | 2017-07-18 |
Family
ID=42028361
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SM20170295T SMT201700295T1 (it) | 2010-01-14 | 2011-01-13 | Ceppo batterico ospite che esprime dsbc ricombinante ed avente attività tsp ridotta |
Country Status (23)
| Country | Link |
|---|---|
| US (2) | US8969038B2 (it) |
| EP (1) | EP2523969B1 (it) |
| JP (1) | JP5960608B2 (it) |
| KR (1) | KR101729361B1 (it) |
| CN (2) | CN102712679B (it) |
| AU (2) | AU2011206587A1 (it) |
| CA (1) | CA2786336C (it) |
| CY (1) | CY1118990T1 (it) |
| DK (1) | DK2523969T3 (it) |
| ES (1) | ES2627826T3 (it) |
| GB (1) | GB201000591D0 (it) |
| HK (1) | HK1206785A1 (it) |
| HR (1) | HRP20170852T1 (it) |
| HU (1) | HUE033555T2 (it) |
| LT (1) | LT2523969T (it) |
| ME (1) | ME02752B (it) |
| PL (1) | PL2523969T3 (it) |
| PT (1) | PT2523969T (it) |
| RS (1) | RS56159B1 (it) |
| SG (1) | SG182280A1 (it) |
| SI (1) | SI2523969T1 (it) |
| SM (1) | SMT201700295T1 (it) |
| WO (1) | WO2011086139A1 (it) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2774692C (en) | 2009-09-24 | 2017-12-19 | Ucb Pharma S.A. | Bacterial strain for recombinant protein expression, having protease deficient degp retaining chaperone activity, and knocked out tsp and ptr genes |
| GB201000590D0 (en) | 2010-01-14 | 2010-03-03 | Ucb Pharma Sa | Bacterial host strain |
| GB201000591D0 (en) * | 2010-01-14 | 2010-03-03 | Ucb Pharma Sa | Bacterial hoist strain |
| GB201000587D0 (en) | 2010-01-14 | 2010-03-03 | Ucb Pharma Sa | Bacterial hoist strain |
| GB201001791D0 (en) | 2010-02-03 | 2010-03-24 | Ucb Pharma Sa | Process for obtaining antibodies |
| GB201012599D0 (en) | 2010-07-27 | 2010-09-08 | Ucb Pharma Sa | Process for purifying proteins |
| SMT201800068T1 (it) * | 2011-07-13 | 2018-03-08 | Ucb Biopharma Sprl | Ceppo ospite batterico che esprime dsbc ricombinante |
| EP2546267A1 (en) * | 2011-07-13 | 2013-01-16 | UCB Pharma S.A. | Bacterial host strain expressing recombinant DsbC |
| GB201208367D0 (en) | 2012-05-14 | 2012-06-27 | Ucb Pharma Sa | Biological product |
| US11306328B2 (en) | 2013-07-26 | 2022-04-19 | President And Fellows Of Harvard College | Genome engineering |
| JP6807327B2 (ja) | 2014-12-22 | 2021-01-06 | ユーシービー バイオファルマ エスアールエル | タンパク質製造 |
| RU2018128784A (ru) | 2016-01-27 | 2020-02-27 | МЕДИММЬЮН, ЭлЭлСи | Способы получения антител с заданным профилем гликозилирования |
| EP3824906A1 (en) | 2016-12-21 | 2021-05-26 | Amgen Inc. | Anti-tnf alpha antibody formulations |
| EP3774926A1 (en) | 2018-04-05 | 2021-02-17 | Bio-Rad ABD Serotec GmbH | Display systems for proteins of interest |
| CN113260626B (zh) | 2018-11-05 | 2024-09-13 | 豪夫迈·罗氏有限公司 | 在原核宿主细胞中产生双链蛋白质的方法 |
| CN113874518B (zh) | 2019-03-18 | 2025-04-25 | 生物辐射Abd瑟罗泰克有限公司 | 保护含SpyTag的周质融合蛋白免于蛋白酶Tsp和OmpT降解 |
| ES3026707T3 (en) * | 2019-08-05 | 2025-06-12 | Wacker Chemie Ag | Bacterial strain for releasing a recombinant protein in a fermentation method |
| KR20240021234A (ko) | 2021-06-10 | 2024-02-16 | 펠리칸 테크놀로지 홀딩스, 인크. | 재조합 단백질 발현을 위한 박테리아 숙주 |
Family Cites Families (50)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5264365A (en) | 1990-11-09 | 1993-11-23 | Board Of Regents, The University Of Texas System | Protease-deficient bacterial strains for production of proteolytically sensitive polypeptides |
| US5508192A (en) | 1990-11-09 | 1996-04-16 | Board Of Regents, The University Of Texas System | Bacterial host strains for producing proteolytically sensitive polypeptides |
| GB9215540D0 (en) | 1992-07-22 | 1992-09-02 | Celltech Ltd | Protein expression system |
| US5304472A (en) | 1992-11-20 | 1994-04-19 | Genentech, Inc. | Method of controlling polypeptide production in bacterial cells |
| US5639635A (en) | 1994-11-03 | 1997-06-17 | Genentech, Inc. | Process for bacterial production of polypeptides |
| AU2660397A (en) | 1996-04-05 | 1997-10-29 | Board Of Regents, The University Of Texas System | Methods for producing soluble, biologically-active disulfide bond-containing eukaryotic proteins in bacterial cells |
| US6083715A (en) | 1997-06-09 | 2000-07-04 | Board Of Regents, The University Of Texas System | Methods for producing heterologous disulfide bond-containing polypeptides in bacterial cells |
| JP2000083670A (ja) | 1998-09-09 | 2000-03-28 | Hsp Kenkyusho:Kk | ＤｓｂＡ／ＤｓｂＢ／ＤｓｂＣ／ＤｓｂＤ発現プラスミド |
| JP2003503074A (ja) | 1999-06-29 | 2003-01-28 | シガ・テクノロジーズ，インコーポレイテッド | Ｄｅｇｐプロテアーゼインヒビター類の分析方法 |
| GB0006398D0 (en) | 2000-03-16 | 2000-05-03 | Novartis Ag | Organic compounds |
| GB0013810D0 (en) | 2000-06-06 | 2000-07-26 | Celltech Chiroscience Ltd | Biological products |
| EP1314037A2 (en) | 2000-09-01 | 2003-05-28 | Biogen, Inc. | Methods of designing and producing compounds having improved binding affinity for cd154 or other trimeric proteins |
| AU8867501A (en) | 2000-09-01 | 2002-03-13 | Biogen Inc | Co-crystal structure of monoclonal antibody 5c8 and cd154, and use thereof in drug design |
| US7041479B2 (en) | 2000-09-06 | 2006-05-09 | The Board Of Trustess Of The Leland Stanford Junior University | Enhanced in vitro synthesis of active proteins containing disulfide bonds |
| BR0116680A (pt) | 2000-12-14 | 2005-04-05 | Genentech Inc | Linhagem de e. coli e método de produção de um polipeptìdeo |
| EP1356052B1 (en) * | 2000-12-14 | 2008-08-20 | Genentech, Inc. | Production of full antibodies in procaryotic cells |
| TWI327597B (en) | 2001-08-01 | 2010-07-21 | Centocor Inc | Anti-tnf antibodies, compositions, methods and uses |
| EP1427744B1 (en) * | 2001-08-27 | 2007-12-26 | Genentech, Inc. | A system for antibody expression and assembly |
| GB0124317D0 (en) | 2001-10-10 | 2001-11-28 | Celltech R&D Ltd | Biological products |
| ATE420964T1 (de) | 2001-11-16 | 2009-01-15 | Biogen Idec Inc | Polycistronische expression von antikörpern in cho zellen |
| GB0129105D0 (en) | 2001-12-05 | 2002-01-23 | Celltech R&D Ltd | Expression control using variable intergenic sequences |
| CN1678634A (zh) | 2002-06-28 | 2005-10-05 | 多曼蒂斯有限公司 | 免疫球蛋白单个变体抗原结合区及其特异性构建体 |
| GB0224082D0 (en) | 2002-10-16 | 2002-11-27 | Celltech R&D Ltd | Biological products |
| MXPA05004677A (es) | 2002-10-31 | 2005-11-17 | Genentech Inc | Metodos y composiciones para aumentar la produccion de anticuerpos. |
| WO2004051268A1 (en) | 2002-12-03 | 2004-06-17 | Celltech R & D Limited | Assay for identifying antibody producing cells |
| GB0303337D0 (en) | 2003-02-13 | 2003-03-19 | Celltech R&D Ltd | Biological products |
| DK1639014T3 (da) | 2003-06-13 | 2011-01-17 | Biogen Idec Inc | Aglycosyl-anti-CD154 (CD40-ligand)-antistoffer og anvendelser deraf |
| JP5087274B2 (ja) | 2003-06-30 | 2012-12-05 | ドマンティス リミテッド | ポリペプチド |
| GB0315450D0 (en) | 2003-07-01 | 2003-08-06 | Celltech R&D Ltd | Biological products |
| GB0315457D0 (en) | 2003-07-01 | 2003-08-06 | Celltech R&D Ltd | Biological products |
| JP4944608B2 (ja) | 2003-07-26 | 2012-06-06 | バイオジェン・アイデック・エムエイ・インコーポレイテッド | 改良された抗原結合親和性を有する、変更された抗体 |
| AU2005287406B2 (en) | 2004-07-26 | 2011-08-18 | Biogen Ma Inc. | Anti-CD154 antibodies |
| WO2006028936A2 (en) | 2004-09-02 | 2006-03-16 | Genentech, Inc. | Heteromultimeric molecules |
| US7563443B2 (en) | 2004-09-17 | 2009-07-21 | Domantis Limited | Monovalent anti-CD40L antibody polypeptides and compositions thereof |
| GB0425534D0 (en) | 2004-11-19 | 2004-12-22 | Celltech R&D Ltd | Process for obtaining antibodies |
| GB0520169D0 (en) | 2005-10-04 | 2005-11-09 | Celltech R&D Ltd | Biological products |
| BRPI0809042B1 (pt) | 2007-03-22 | 2021-08-31 | Biogen Ma Inc. | Proteína de ligação a cd154 isolada, seu uso, e composição |
| US7662587B1 (en) | 2009-03-05 | 2010-02-16 | E. I. Du Pont De Nemours And Company | Gene knockout mutations that increase peptide production |
| CA2774692C (en) * | 2009-09-24 | 2017-12-19 | Ucb Pharma S.A. | Bacterial strain for recombinant protein expression, having protease deficient degp retaining chaperone activity, and knocked out tsp and ptr genes |
| US8470552B2 (en) | 2009-10-12 | 2013-06-25 | Keck Graduate Institute | Strategy to reduce lactic acid production and control PH in animal cell culture |
| KR101968766B1 (ko) | 2009-11-05 | 2019-04-12 | 제넨테크, 인크. | 이종 폴리펩티드의 분비를 위한 방법 및 조성물 |
| GB201000590D0 (en) | 2010-01-14 | 2010-03-03 | Ucb Pharma Sa | Bacterial host strain |
| GB201000588D0 (en) | 2010-01-14 | 2010-03-03 | Ucb Pharma Sa | Bacterial host strain |
| GB201000587D0 (en) | 2010-01-14 | 2010-03-03 | Ucb Pharma Sa | Bacterial hoist strain |
| GB201000591D0 (en) * | 2010-01-14 | 2010-03-03 | Ucb Pharma Sa | Bacterial hoist strain |
| GB201001791D0 (en) | 2010-02-03 | 2010-03-24 | Ucb Pharma Sa | Process for obtaining antibodies |
| GB201012599D0 (en) | 2010-07-27 | 2010-09-08 | Ucb Pharma Sa | Process for purifying proteins |
| EP2546267A1 (en) | 2011-07-13 | 2013-01-16 | UCB Pharma S.A. | Bacterial host strain expressing recombinant DsbC |
| SMT201800068T1 (it) | 2011-07-13 | 2018-03-08 | Ucb Biopharma Sprl | Ceppo ospite batterico che esprime dsbc ricombinante |
| GB201208367D0 (en) | 2012-05-14 | 2012-06-27 | Ucb Pharma Sa | Biological product |
-
2010
- 2010-01-14 GB GBGB1000591.6A patent/GB201000591D0/en not_active Ceased
-
2011
- 2011-01-13 EP EP11701045.4A patent/EP2523969B1/en active Active
- 2011-01-13 PL PL11701045T patent/PL2523969T3/pl unknown
- 2011-01-13 SG SG2012047676A patent/SG182280A1/en unknown
- 2011-01-13 US US13/522,083 patent/US8969038B2/en active Active
- 2011-01-13 JP JP2012548441A patent/JP5960608B2/ja active Active
- 2011-01-13 WO PCT/EP2011/050416 patent/WO2011086139A1/en not_active Ceased
- 2011-01-13 RS RS20170626A patent/RS56159B1/sr unknown
- 2011-01-13 KR KR1020127021021A patent/KR101729361B1/ko active Active
- 2011-01-13 SI SI201131205A patent/SI2523969T1/sl unknown
- 2011-01-13 HU HUE11701045A patent/HUE033555T2/hu unknown
- 2011-01-13 LT LTEP11701045.4T patent/LT2523969T/lt unknown
- 2011-01-13 PT PT117010454T patent/PT2523969T/pt unknown
- 2011-01-13 SM SM20170295T patent/SMT201700295T1/it unknown
- 2011-01-13 DK DK11701045.4T patent/DK2523969T3/en active
- 2011-01-13 HR HRP20170852TT patent/HRP20170852T1/hr unknown
- 2011-01-13 CA CA2786336A patent/CA2786336C/en active Active
- 2011-01-13 CN CN201180005978.1A patent/CN102712679B/zh active Active
- 2011-01-13 CN CN201410591520.4A patent/CN104328079A/zh active Pending
- 2011-01-13 ES ES11701045.4T patent/ES2627826T3/es active Active
- 2011-01-13 AU AU2011206587A patent/AU2011206587A1/en not_active Abandoned
- 2011-01-13 ME MEP-2017-127A patent/ME02752B/me unknown
-
2013
- 2013-03-25 HK HK15107468.1A patent/HK1206785A1/xx unknown
-
2015
- 2015-02-27 US US14/633,294 patent/US9493559B2/en active Active
-
2017
- 2017-04-12 AU AU2017202434A patent/AU2017202434B2/en active Active
- 2017-06-22 CY CY20171100667T patent/CY1118990T1/el unknown
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