SMT202000537T1 - Espressione aumentata di una proteina ricombinante mediante l'uso di un promotore ibrido chef1 - Google Patents

Espressione aumentata di una proteina ricombinante mediante l'uso di un promotore ibrido chef1

Info

Publication number
SMT202000537T1
SMT202000537T1 SM20200537T SMT202000537T SMT202000537T1 SM T202000537 T1 SMT202000537 T1 SM T202000537T1 SM 20200537 T SM20200537 T SM 20200537T SM T202000537 T SMT202000537 T SM T202000537T SM T202000537 T1 SMT202000537 T1 SM T202000537T1
Authority
SM
San Marino
Prior art keywords
hybrid
protein expression
recombinant protein
improved recombinant
chef1 promoter
Prior art date
Application number
SM20200537T
Other languages
English (en)
Inventor
Howard R Clarke
Original Assignee
Agc Biologics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agc Biologics Inc filed Critical Agc Biologics Inc
Publication of SMT202000537T1 publication Critical patent/SMT202000537T1/it

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
SM20200537T 2013-03-12 2014-03-11 Espressione aumentata di una proteina ricombinante mediante l'uso di un promotore ibrido chef1 SMT202000537T1 (it)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361777603P 2013-03-12 2013-03-12
EP14727377.5A EP2971005B1 (en) 2013-03-12 2014-03-11 Improved recombinant protein expression using a hybrid chef1 promoter
PCT/US2014/023661 WO2014164869A1 (en) 2013-03-12 2014-03-11 Improved recombinant protein expression using a hybrid chef1 promoter

Publications (1)

Publication Number Publication Date
SMT202000537T1 true SMT202000537T1 (it) 2020-11-10

Family

ID=50846904

Family Applications (1)

Application Number Title Priority Date Filing Date
SM20200537T SMT202000537T1 (it) 2013-03-12 2014-03-11 Espressione aumentata di una proteina ricombinante mediante l'uso di un promotore ibrido chef1

Country Status (18)

Country Link
US (4) US9297024B2 (it)
EP (2) EP2971005B1 (it)
KR (1) KR102365484B1 (it)
CN (2) CN105209625B (it)
AU (1) AU2014248936B2 (it)
CA (1) CA2904125C (it)
CY (1) CY1123336T1 (it)
DK (1) DK2971005T3 (it)
ES (1) ES2811648T3 (it)
HR (1) HRP20201442T1 (it)
HU (1) HUE050362T2 (it)
LT (1) LT2971005T (it)
NZ (1) NZ711654A (it)
PL (1) PL2971005T3 (it)
PT (1) PT2971005T (it)
SI (1) SI2971005T1 (it)
SM (1) SMT202000537T1 (it)
WO (1) WO2014164869A1 (it)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102365484B1 (ko) * 2013-03-12 2022-02-18 에이쥐씨 바이올로직스, 인크. 하이브리드 chef1 프로모터를 이용한 향상된 재조합 단백질 발현
AR095196A1 (es) * 2013-03-15 2015-09-30 Regeneron Pharma Medio de cultivo celular libre de suero
US10745730B2 (en) 2015-09-28 2020-08-18 The Trustees Of Columbia University Of The City Of New York Modified bacterial protein expression system
GB201603577D0 (en) 2016-03-01 2016-04-13 Oxford Genetics Ltd Promoter
CN107043773B (zh) * 2017-02-08 2018-09-18 四川丰讯科技发展有限公司 一种提高哺乳动物细胞中外源基因表达水平的基因片段、重组载体及其用途
US11713468B2 (en) 2017-08-02 2023-08-01 Dna Twopointo Inc. DNA vectors and elements for sustained gene expression in eukaryotic cells
CA3088880A1 (en) * 2018-01-10 2019-07-18 Cmc Icos Biologics, Inc. Bidirectional chef1 vectors
US12297451B1 (en) 2019-10-25 2025-05-13 Regeneron Pharmaceuticals, Inc. Cell culture medium
CN120187857A (zh) * 2023-01-10 2025-06-20 南京传奇生物科技有限公司 调控序列及其用途

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5888809A (en) 1997-05-01 1999-03-30 Icos Corporation Hamster EF-1α transcriptional regulatory DNA
EP1492874B1 (en) * 2002-03-29 2011-01-19 XOMA Technology Ltd. Multigenic vector plasmids and methods for increasing expression of recombinant polypeptides
KR100533794B1 (ko) 2003-10-02 2005-12-07 주식회사 프로젠 인간 난포자극 호르몬을 대량으로 생산하는 방법
EP1819829A1 (en) * 2004-12-08 2007-08-22 ICOS Corporation Recombinant method for making multimeric proteins
EP1969127B8 (en) * 2005-12-21 2017-12-13 Aptevo BioTherapeutics LLC Method of producing biologically active vitamin k dependent proteins by recombinant methods
WO2007081336A1 (en) * 2006-01-13 2007-07-19 Five Prime Therapeutics, Inc. Mammalian vectors for high-level expression of recombinant proteins
CA2656558A1 (en) * 2006-06-30 2008-01-10 The Regents Of The University Of Michigan Method of producing factor viii proteins by recombinant methods
CA2771410C (en) * 2009-08-06 2020-10-06 Cmc Icos Biologics, Inc. Methods for improving recombinant protein expression comprising reduction in translation efficiency of a selectable marker protein
WO2012052878A1 (en) 2010-10-19 2012-04-26 Vascular Biogenics Ltd. Isolated polynucleotides and nucleic acid constructs for directing expression of a gene-of-interest in cells
KR102365484B1 (ko) * 2013-03-12 2022-02-18 에이쥐씨 바이올로직스, 인크. 하이브리드 chef1 프로모터를 이용한 향상된 재조합 단백질 발현

Also Published As

Publication number Publication date
KR102365484B1 (ko) 2022-02-18
US20140273208A1 (en) 2014-09-18
CN105209625B (zh) 2019-03-29
KR20150126399A (ko) 2015-11-11
AU2014248936B2 (en) 2020-07-09
DK2971005T3 (da) 2020-08-24
CN110042122A (zh) 2019-07-23
CY1123336T1 (el) 2021-12-31
EP2971005A1 (en) 2016-01-20
US20210340560A1 (en) 2021-11-04
US9957525B2 (en) 2018-05-01
CA2904125A1 (en) 2014-10-09
AU2014248936A1 (en) 2015-09-24
HUE050362T2 (hu) 2020-11-30
SI2971005T1 (sl) 2020-10-30
HK1220491A1 (en) 2017-05-05
US20190071691A1 (en) 2019-03-07
WO2014164869A1 (en) 2014-10-09
NZ711654A (en) 2021-07-30
US20160177335A1 (en) 2016-06-23
EP3770263A1 (en) 2021-01-27
EP2971005B1 (en) 2020-06-17
HRP20201442T1 (hr) 2020-12-11
CA2904125C (en) 2022-06-07
LT2971005T (lt) 2020-10-12
CN105209625A (zh) 2015-12-30
ES2811648T3 (es) 2021-03-12
PT2971005T (pt) 2020-09-01
US9297024B2 (en) 2016-03-29
PL2971005T3 (pl) 2020-12-14

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