SU128564A1 - The method of cleaning adrenocorticotropic hormone - Google Patents
The method of cleaning adrenocorticotropic hormoneInfo
- Publication number
- SU128564A1 SU128564A1 SU614022A SU614022A SU128564A1 SU 128564 A1 SU128564 A1 SU 128564A1 SU 614022 A SU614022 A SU 614022A SU 614022 A SU614022 A SU 614022A SU 128564 A1 SU128564 A1 SU 128564A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- cleaning
- ballast
- adrenocorticotropic hormone
- acetone
- added
- Prior art date
Links
- 238000000034 method Methods 0.000 title description 5
- 239000000275 Adrenocorticotropic Hormone Substances 0.000 title description 2
- 101800000414 Corticotropin Proteins 0.000 title description 2
- 102100027467 Pro-opiomelanocortin Human genes 0.000 title description 2
- IDLFZVILOHSSID-OVLDLUHVSA-N corticotropin Chemical compound C([C@@H](C(=O)N[C@@H](CO)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC=1NC=NC=1)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)NCC(=O)N[C@@H](CCCCN)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](C(C)C)C(=O)NCC(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CC(N)=O)C(=O)NCC(=O)N[C@@H](C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CO)C(=O)N[C@@H](C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC=1C=CC=CC=1)C(O)=O)NC(=O)[C@@H](N)CO)C1=CC=C(O)C=C1 IDLFZVILOHSSID-OVLDLUHVSA-N 0.000 title description 2
- 229960000258 corticotropin Drugs 0.000 title description 2
- 238000004140 cleaning Methods 0.000 title 1
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 10
- 102000004169 proteins and genes Human genes 0.000 claims description 4
- 108090000623 proteins and genes Proteins 0.000 claims description 4
- 238000004062 sedimentation Methods 0.000 claims description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 230000002378 acidificating effect Effects 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 229940088597 hormone Drugs 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
Landscapes
- Peptides Or Proteins (AREA)
Description
Изобретение относитс к сокращенному способу очистки кислого ацетонировэнного порошка адренокортикотропного гормона.This invention relates to an abbreviated method for the purification of acidic acetonated adrenocorticotropic hormone powder.
Известен способ очистки препарата путем отделени белкового балласта в изоэле трической его точке при рН 5,0-5,5.A known method for the purification of a preparation by separating protein ballast in its iso-electric point at pH 5.0-5.5.
Предлагаемый способ отличаетс от известного тем, что дл понижени поверхностного нат жени и отделени балласта перед осаждением прибавл ют 1,3 объема ацетона, а белковый балласт осаждают при рН 6-7, что увеличивает выход препарата и улучшает его качество.The proposed method differs from the known one in that 1.3 volumes of acetone are added before sedimentation to decrease surface tension and separate ballast, and protein ballast is precipitated at pH 6-7, which increases the yield of the preparation and improves its quality.
Способ осуществл етс следующим образом . Кислый ацетонирванный порошок раствор ют в двадцатикратном количестве дистиллированной воды, подкисленной ОД0 ,15%.ой сол ной кислотой, прибавл ют 0,1% хлористого натри , подогревают на вод нс бане до температуры 90-96 С и при этой темлературе выдерживают 6О мин, после чего быстро охлаждают до комнатной температуры , добавл ют 1,3 объема ацетона к раствору, подщелачивают до рН 1О,т.е. The method is carried out as follows. The acidic acetonated powder is dissolved in a twenty-fold amount of distilled water, acidified with OD0, 15% hydrochloric acid, 0.1% sodium chloride is added, heated to a temperature of 90–96 ° C in an aqueous bath and maintained at this temperature for 6O minutes, after which it is rapidly cooled to room temperature, 1.3 volumes of acetone are added to the solution, alkalinized to pH 1O, i.e.
до полного растворени осадка и подкисл ют солной кислотой до рН 6-7.until the precipitate is completely dissolved and acidified with hydrochloric acid to pH 6-7.
Раствор центрифугируют. Балласт подвергают первой обработке, раствор его в дес тикратном количестве дистиллированной воды по КОПу, затем добавл ют 1,3 объема ацетона и подщелачивают до рН 10 (до полного растворени осадка) подкисл ют сол ной кислотой до рН 6-7 и центрифугируют . Балласт, полученный при этом, подвергают вторичной обработке, аналогичной первой, но примен растворителей вдво меньше.The solution is centrifuged. The ballast is subjected to the first treatment, its solution in a tenfold amount of distilled water in KOP, then 1.3 volumes of acetone are added and alkalinized to pH 10 (until the precipitate is completely dissolved) is acidified with hydrochloric acid to pH 6-7 and centrifuged. The ballast obtained in this case, is subjected to secondary treatment, similar to the first, but the use of solvents is two times less.
Все три порции раствора гормона объедин ют , осаждают охлажденным ацетоном и дальше обрабатывают как обычно.All three portions of the hormone solution are combined, precipitated with chilled acetone and further processed as usual.
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU614022A SU128564A1 (en) | 1958-12-15 | 1958-12-15 | The method of cleaning adrenocorticotropic hormone |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU614022A SU128564A1 (en) | 1958-12-15 | 1958-12-15 | The method of cleaning adrenocorticotropic hormone |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU128564A1 true SU128564A1 (en) | 1977-03-25 |
Family
ID=20437014
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU614022A SU128564A1 (en) | 1958-12-15 | 1958-12-15 | The method of cleaning adrenocorticotropic hormone |
Country Status (1)
| Country | Link |
|---|---|
| SU (1) | SU128564A1 (en) |
-
1958
- 1958-12-15 SU SU614022A patent/SU128564A1/en active
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