SU891777A1 - Method of preparing asperase enzymic compound - Google Patents
Method of preparing asperase enzymic compound Download PDFInfo
- Publication number
- SU891777A1 SU891777A1 SU802878326A SU2878326A SU891777A1 SU 891777 A1 SU891777 A1 SU 891777A1 SU 802878326 A SU802878326 A SU 802878326A SU 2878326 A SU2878326 A SU 2878326A SU 891777 A1 SU891777 A1 SU 891777A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- asperase
- ethanol
- ratio
- water
- enzymic
- Prior art date
Links
- 108010044458 asperase Proteins 0.000 title claims description 7
- 238000000034 method Methods 0.000 title description 8
- 150000001875 compounds Chemical class 0.000 title 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 12
- 108090000790 Enzymes Proteins 0.000 claims description 6
- 102000004190 Enzymes Human genes 0.000 claims description 6
- 238000002360 preparation method Methods 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 239000002244 precipitate Substances 0.000 claims description 4
- 240000006439 Aspergillus oryzae Species 0.000 claims description 3
- 235000002247 Aspergillus oryzae Nutrition 0.000 claims description 3
- 238000000605 extraction Methods 0.000 claims description 2
- 238000004090 dissolution Methods 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 238000001226 reprecipitation Methods 0.000 claims 1
- 239000003814 drug Substances 0.000 description 5
- 108010044370 Oraza Proteins 0.000 description 4
- 229940079593 drug Drugs 0.000 description 4
- 229940088598 enzyme Drugs 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 238000001556 precipitation Methods 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 108090000317 Chymotrypsin Proteins 0.000 description 1
- 230000003625 amylolytic effect Effects 0.000 description 1
- 229960002376 chymotrypsin Drugs 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- DPBLXKKOBLCELK-UHFFFAOYSA-N pentan-1-amine Chemical compound CCCCCN DPBLXKKOBLCELK-UHFFFAOYSA-N 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 230000002797 proteolythic effect Effects 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
Landscapes
- Enzymes And Modification Thereof (AREA)
Description
(54) СПОСОБ ПОЛУЧЕНИЯ ФЕРМЕНТНОГО ПРЕПАРАТА АСПЕРАЗЫ(54) METHOD FOR OBTAINING AN ENZYME ASPERASE PRODUCT
Изобретение относитс к новому .способу получени нового ферментного препарата из поверхностной культуры Aspergillus oryzae, которьпй может найти применение в медицине и др. отрасл х. Близкими по специфическому действию на субстраты вл ютс химотрипсин и террилитин. Известен способ получени фермент ного препарата амилолитического действи - Оразы из поверхностной культуры Aspergillus oryzae, который включает экстракцию водой,осаждение этанолом при отношении объема 1:2, растворение полученного осадка в вод при отношении веса 1:3 и осаждение целевого продукта этанолом при отношении 1:(1-3) . Первый осадок в процессе получени Оразы, .полученный осаждением этанолом при отношении :2, содержит помимо целевого амилолнтического фермента и протес.пнти.ческин. Полученный продукт - препарат | 0раза обладаетвыраженным амилолитическим действием и используетс как лекарственное средство дл лечени желудочно-кишечных заболеваний l1 Однако известный способ не обеспечивает возможности получени нового ферментного препарата - асперазы, хот препарат Ораза, получаемый из этого же сырь зказанным способом содержит небольшие примеси продукта ферментативной природы, обладающего протеолитической активностью (4050 ед/г). Таким образом известный способ не обеспечивает возможности комплексного использовани сырь , тогда как в принципе возможно получение пре парата Оразы и препарата асперазы по единой технологической схеме. Цель изобретени - разработка нового способа получени нового ферментного препарата асперазы, расшир ющего арсенал лекарственных средствThe invention relates to a new method of obtaining a new enzyme preparation from a surface culture of Aspergillus oryzae, which can be used in medicine and other fields. Similar to the specific effect on substrates are chymotrypsin and terurilitin. A method of obtaining an enzyme preparation of amylolytic action — Oraza from Aspergillus oryzae surface culture, which involves extraction with water, precipitation with ethanol at a ratio of 1: 2, dissolving the precipitate obtained in water at a ratio of 1: 3, and precipitating the target product with ethanol at a ratio of 1: (1-3). The first precipitate in the process of obtaining Oraza, obtained by precipitation with ethanol with a ratio of: 2, contains, in addition to the target amylamine, enzyme and protes. The resulting product - the drug | However, the known method does not provide the possibility of obtaining an asperase enzyme preparation, although the Oraza preparation obtained from the same raw material contains in this manner small impurities of an enzymatic product with proteolytic activity. (4050 u / g). Thus, the known method does not provide the possibility of complex use of raw materials, whereas, in principle, it is possible to obtain the Oraza drug and the asperase drug according to a single technological scheme. The purpose of the invention is to develop a new method of obtaining a new enzyme preparation asperase, expanding the arsenal of drugs.
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU802878326A SU891777A1 (en) | 1980-01-22 | 1980-01-22 | Method of preparing asperase enzymic compound |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU802878326A SU891777A1 (en) | 1980-01-22 | 1980-01-22 | Method of preparing asperase enzymic compound |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU891777A1 true SU891777A1 (en) | 1981-12-23 |
Family
ID=20875859
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU802878326A SU891777A1 (en) | 1980-01-22 | 1980-01-22 | Method of preparing asperase enzymic compound |
Country Status (1)
| Country | Link |
|---|---|
| SU (1) | SU891777A1 (en) |
-
1980
- 1980-01-22 SU SU802878326A patent/SU891777A1/en active
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