TH290B - Process for producing L-glutamic acid By fermentation - Google Patents

Abstract

Method for producing L-Glutamic acid by fermentation process This method consists of Inoculation in an airborne condition by Using a mutated microorganism of Genus Grevibacterium and Corynebacterium which has the ability to resist Tolerant of didoginine and/or tubercidine antibiotics and can produce L-glutamic acid in glucose-containing culture medium. It is a source of carbon in the presence of excess biodine, i.e. more than 100 ug/l, with the addition of nonionic surface reagents, including the L-glutamic acid separation process. Mid, which is accumulated in agar

Claims (5)

1. A method for producing L-glutamic acid through fermentation. This process involves aerobic cultivation using mutated strains of Brevibacterium and Corynebacterium that are resistant to the antibiotics dodoinine and/or tubercidine and can produce L-glutamic acid in a culture medium containing glucose as a carbon source under an excess biodine concentration (more than 100 µg/L) and with the addition of a surface reagent. 2. The procedure under claim 1, which includes the isolation of L-glutamide accumulated in the culture medium solution. 3. The process under claim 1 or 2, in which the nonionic surface reactant is a fatty acid ester of polyoxyethylene sorbitame. 4. The procedure under claim 1 or 2 involves the use of mutated microorganisms of Erevibacterium lactofermentum, Frevibacterium flavum, Corynebacterium glutamicum, or Corynebacterium acetoacidoplilum. 5. According to claim 1 or 2, the mutated microorganisms used in the production of L-glutamic acid are: Brevibacterium lactofermentum. FERM BP-5812 (FERMGP-74), Brevibacterium lactofermentum. FERM P-5811 (FERM8P-73), or Corynebacterium glutamicum. FERM P-5819 (FERM8P-75). (5 claims, 1 page, 0 images)