TH33568A - Mutanase-Containing Oral Compositions - Google Patents

Abstract

DC60 (08/07/40) is an oral mixture containing mutanase derived from cultured mutanase-inducing microorganisms of the genus Bacillus that are egg-negative. This oral mixture is effective in inhibiting oral plaque formation, and the mutanase exhibits commercially acceptable stability within the mixture.

Claims (3)

1. Oral mixtures containing mutanases prepared from cultures of mutanas-inducing microorganisms in the genus Bacillus that produce negative oocytes. 2. Oral mixtures containing mutanase exhibiting the following natural chemical properties (1) to 9): 1) Function: Ability to break down the (formula)-1,3-glucosidic chain of mutan. 2) Substrate characteristic: Ability to efficiently break down mutan. 3) Optimal pH: pH 4 to 4.5 when reacting on a mutan substrate at 35°C for 10 minutes. 4) pH range for stability: pH 4 to 10 when maintained at 25°C for 24 hours. 5) Optimal temperature: 50 to 65°C when reacting on a mutan substrate at pH 5. 6) Thermal stability: Enzyme activity remains stable. 7) Effects of metal ions: Mercury and silver showed inhibitory effects on the mutan substrate. 8) Inhibitory effect: p-chloromercurybenzonic acid showed inhibitory effect on the mutan substrate. 9) Molecular weight: Approximately 140,000 to approximately 160,000, as determined by translocation to the electrodes of the SDS-polyacrylamide gel. 3. A mixture for the mouth of claim 1 or claim 2 consisting of a deck of cards (3 claims, 1 page, 12 figures).